WO2000052162A3 - Eukaryotic peptide uptake system for transportation of enkephalins - Google Patents

Eukaryotic peptide uptake system for transportation of enkephalins Download PDF

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Publication number
WO2000052162A3
WO2000052162A3 PCT/US2000/005158 US0005158W WO0052162A3 WO 2000052162 A3 WO2000052162 A3 WO 2000052162A3 US 0005158 W US0005158 W US 0005158W WO 0052162 A3 WO0052162 A3 WO 0052162A3
Authority
WO
WIPO (PCT)
Prior art keywords
gly
enkephalins
transportation
opt1
pentapeptides
Prior art date
Application number
PCT/US2000/005158
Other languages
French (fr)
Other versions
WO2000052162A2 (en
Inventor
Jeffrey M Becker
Melinda Hauser
Amy Donhardt
David Barnes
Original Assignee
Univ Tennessee Res Corp
Jeffrey M Becker
Melinda Hauser
Amy Donhardt
David Barnes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Tennessee Res Corp, Jeffrey M Becker, Melinda Hauser, Amy Donhardt, David Barnes filed Critical Univ Tennessee Res Corp
Priority to AU33858/00A priority Critical patent/AU3385800A/en
Priority to CA002364584A priority patent/CA2364584A1/en
Publication of WO2000052162A2 publication Critical patent/WO2000052162A2/en
Publication of WO2000052162A3 publication Critical patent/WO2000052162A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

An oligopeptide transporter in the yeast Saccharomyces cerevisiae mediates the uptake of tetra- and pentapeptides, including the endogenous opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly-Phe-Met). The transporter is encoded by the gene OPT1. The system is specific for tetra- and pentapeptides and can be inhibited by the opioid receptor antagonists naloxone and naltrexone. Vectors allowing expression of OPT1 and methods of use are disclosed. Treatment of OPT1p with toxic enkephalins as an antifungal method is also disclosed.
PCT/US2000/005158 1999-03-01 2000-03-01 Eukaryotic peptide uptake system for transportation of enkephalins WO2000052162A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU33858/00A AU3385800A (en) 1999-03-01 2000-03-01 Eukaryotic peptide uptake system for transportation of enkephalins
CA002364584A CA2364584A1 (en) 1999-03-01 2000-03-01 Eukaryotic peptide uptake system for transportation of enkephalins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12231299P 1999-03-01 1999-03-01
US60/122,312 1999-03-01

Publications (2)

Publication Number Publication Date
WO2000052162A2 WO2000052162A2 (en) 2000-09-08
WO2000052162A3 true WO2000052162A3 (en) 2001-01-04

Family

ID=22401973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/005158 WO2000052162A2 (en) 1999-03-01 2000-03-01 Eukaryotic peptide uptake system for transportation of enkephalins

Country Status (3)

Country Link
AU (1) AU3385800A (en)
CA (1) CA2364584A1 (en)
WO (1) WO2000052162A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012013505A2 (en) * 2010-07-28 2012-02-02 Unilever Plc Composition

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034950A1 (en) * 1997-02-07 1998-08-13 The University Of Tennessee Research Corporation Isolated candida albicans oligopeptide transporter gene

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998034950A1 (en) * 1997-02-07 1998-08-13 The University Of Tennessee Research Corporation Isolated candida albicans oligopeptide transporter gene

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HAUSER M ET AL: "Enkephalins are transported by a novel eukaryotic peptide uptake system.", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 275, no. 5, 4 February 2000 (2000-02-04), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 3037 - 3041, XP002144948, ISSN: 0021-9258 *
LUBKOWITZ MARK A ET AL: "An oligopeptide transport gene from Candida albicans.", MICROBIOLOGY (READING), vol. 143, no. 2, 1997, pages 387 - 396, XP000929716, ISSN: 1350-0872 *
LUBKOWITZ MARK A ET AL: "Schizosaccharomyces pombe isp4 encodes a transporter representing a novel family of oligopeptide transporters.", MOLECULAR MICROBIOLOGY, vol. 28, no. 4, May 1998 (1998-05-01), pages 729 - 741, XP000929697, ISSN: 0950-382X *
TYNKKYNEN S ET AL: "Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis.", JOURNAL OF BACTERIOLOGY 1993 RES. & DEV. CENT., VALIO LTD., PO BOX 390, SF-00101 HELSINKI, FINLAND, vol. 175, no. 23, pages 7523 - 7532, XP000929851 *

Also Published As

Publication number Publication date
AU3385800A (en) 2000-09-21
WO2000052162A2 (en) 2000-09-08
CA2364584A1 (en) 2000-09-08

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