WO2000050644A3 - Procedes de purification d'adn a l'aide de sondes de capture immobilisees - Google Patents

Procedes de purification d'adn a l'aide de sondes de capture immobilisees Download PDF

Info

Publication number
WO2000050644A3
WO2000050644A3 PCT/US2000/004939 US0004939W WO0050644A3 WO 2000050644 A3 WO2000050644 A3 WO 2000050644A3 US 0004939 W US0004939 W US 0004939W WO 0050644 A3 WO0050644 A3 WO 0050644A3
Authority
WO
WIPO (PCT)
Prior art keywords
capture probes
methods
immobilized capture
purifying dna
dna
Prior art date
Application number
PCT/US2000/004939
Other languages
English (en)
Other versions
WO2000050644A2 (fr
Inventor
Christopher P Adams
T Christian Boles
Lawrence Weir
Rahul K Dhanda
Nevin M Summers
Original Assignee
Mosaic Technologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies filed Critical Mosaic Technologies
Priority to JP2000601207A priority Critical patent/JP2002537778A/ja
Priority to AU37083/00A priority patent/AU3708300A/en
Priority to CA002369002A priority patent/CA2369002A1/fr
Priority to EP00915885A priority patent/EP1163371A2/fr
Publication of WO2000050644A2 publication Critical patent/WO2000050644A2/fr
Publication of WO2000050644A3 publication Critical patent/WO2000050644A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés utilisant des dispositifs de purification comprenant un milieu électrophorétique contenant des sondes de capture immobilisées pour la purification de l'ADN.
PCT/US2000/004939 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees WO2000050644A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000601207A JP2002537778A (ja) 1999-02-26 2000-02-25 固定化捕捉プローブを用いたｄｎａの精製方法
AU37083/00A AU3708300A (en) 1999-02-26 2000-02-25 Methods for purifying dna using immobilized capture probes
CA002369002A CA2369002A1 (fr) 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees
EP00915885A EP1163371A2 (fr) 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25946799A 1999-02-26 1999-02-26
US09/259,467 1999-02-26

Publications (2)

Publication Number Publication Date
WO2000050644A2 WO2000050644A2 (fr) 2000-08-31
WO2000050644A3 true WO2000050644A3 (fr) 2001-04-05

Family

ID=22985081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004939 WO2000050644A2 (fr) 1999-02-26 2000-02-25 Procedes de purification d'adn a l'aide de sondes de capture immobilisees

Country Status (5)

Country Link
EP (1) EP1163371A2 (fr)
JP (1) JP2002537778A (fr)
AU (1) AU3708300A (fr)
CA (1) CA2369002A1 (fr)
WO (1) WO2000050644A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4276301B2 (ja) 1997-05-16 2009-06-10 エグザクト サイエンシーズ コーポレイション 固定化プローブを用いる、分子の電気泳動分析
JP2001521169A (ja) * 1997-10-24 2001-11-06 ノースイースタン・ユニバーシティ 広範囲の採集と分析とで高スループットの調整分離のためのマルチチャンネルの微小規模システム
EP1444500B1 (fr) * 2001-10-15 2008-06-11 Biocept, Inc. Biopuce a micropuits
JP2005110503A (ja) * 2003-10-02 2005-04-28 Arkray Inc 核酸の精製方法及びデバイス
EP1685395A2 (fr) 2003-11-05 2006-08-02 EXACT Sciences Corporation Techniques de separation repetitive par affinite et leurs utilisations

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020831A1 (fr) * 1993-03-08 1994-09-15 Norman Wainwright Chromatographie a fibres alignees a usage diagnostique
US5556598A (en) * 1992-09-18 1996-09-17 Amersham International Plc. Device and method for affinity separation
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5556598A (en) * 1992-09-18 1996-09-17 Amersham International Plc. Device and method for affinity separation
WO1994020831A1 (fr) * 1993-03-08 1994-09-15 Norman Wainwright Chromatographie a fibres alignees a usage diagnostique
US5560811A (en) * 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method

Also Published As

Publication number Publication date
EP1163371A2 (fr) 2001-12-19
WO2000050644A2 (fr) 2000-08-31
AU3708300A (en) 2000-09-14
JP2002537778A (ja) 2002-11-12
CA2369002A1 (fr) 2000-08-31

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