WO2000050612A3 - Activateur de proteine c recombinant et ses utilisations - Google Patents

Activateur de proteine c recombinant et ses utilisations Download PDF

Info

Publication number
WO2000050612A3
WO2000050612A3 PCT/US2000/004406 US0004406W WO0050612A3 WO 2000050612 A3 WO2000050612 A3 WO 2000050612A3 US 0004406 W US0004406 W US 0004406W WO 0050612 A3 WO0050612 A3 WO 0050612A3
Authority
WO
WIPO (PCT)
Prior art keywords
pca
protein
preparations
activator
recombinant protein
Prior art date
Application number
PCT/US2000/004406
Other languages
English (en)
Other versions
WO2000050612A2 (fr
Inventor
Yune Zhang Kunes
Maria-Cruz Sanz
Irina A Tumanova
Cynthia A Birr
Philip Q Shi
Pau Bruguera
Juan A Ruiz
Demetrio Sanchez-Martinez
Original Assignee
Instrumentation Lab
Yune Zhang Kunes
Sanz Maria Cruz
Irina A Tumanova
Cynthia A Birr
Philip Q Shi
Pau Bruguera
Juan A Ruiz
Sanchez Martinez Demetrio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instrumentation Lab, Yune Zhang Kunes, Sanz Maria Cruz, Irina A Tumanova, Cynthia A Birr, Philip Q Shi, Pau Bruguera, Juan A Ruiz, Sanchez Martinez Demetrio filed Critical Instrumentation Lab
Priority to AU37032/00A priority Critical patent/AU3703200A/en
Publication of WO2000050612A2 publication Critical patent/WO2000050612A2/fr
Publication of WO2000050612A3 publication Critical patent/WO2000050612A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un activateur de protéine C (PCA) sous forme hautement glycosylée, produit par recombinaison dans de la levure, et présentant des propriétés qui le distinguent des PCA obtenus avec les techniques antérieures. En outre, cette invention concerne des acides nucléiques isolés comportant des séquences nucléotidiques codant des polypeptides qui contiennent des protéines PCA naturelles, ou des variants biosynthétiques de ceux-ci, et concerne également la production de molécules synthétiques codant une protéine PCA provenant du serpent Agkistrodon compartrix compartrix (ACC), ou ses variants biosynthétiques. Par ailleurs, cette invention concerne des préparations protéiques sensiblement pures contenant une protéine PCA, des procédés de production de ces préparations, ainsi que des kits de diagnostic de coagulation sanguine contenant ces préparations.
PCT/US2000/004406 1999-02-22 2000-02-22 Activateur de proteine c recombinant et ses utilisations WO2000050612A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU37032/00A AU3703200A (en) 1999-02-22 2000-02-22 Recombinant protein c activator and uses therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12112199P 1999-02-22 1999-02-22
US60/121,121 1999-02-22

Publications (2)

Publication Number Publication Date
WO2000050612A2 WO2000050612A2 (fr) 2000-08-31
WO2000050612A3 true WO2000050612A3 (fr) 2000-12-14

Family

ID=22394702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004406 WO2000050612A2 (fr) 1999-02-22 2000-02-22 Activateur de proteine c recombinant et ses utilisations

Country Status (2)

Country Link
AU (1) AU3703200A (fr)
WO (1) WO2000050612A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100920297B1 (ko) 2004-10-12 2009-10-08 에프. 호프만-라 로슈 아게 고상 펩티드 합성 방법
CN114814245A (zh) * 2022-06-30 2022-07-29 深圳市帝迈生物技术有限公司 一种基于发色底物法的蛋白c活性测定试剂盒

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0256421A2 (fr) * 1986-08-12 1988-02-24 Phillips Petroleum Company Sécrétion de protéines hétérologues à partir de la levure
WO1989000205A1 (fr) * 1987-07-02 1989-01-12 American National Red Cross Nouvel activateur de proteine c
EP0323722A1 (fr) * 1987-12-18 1989-07-12 Chiron Corporation Compositions et méthodes de production de la fibrolase de crotalidus venom par ADN recombinant
WO1998042850A1 (fr) * 1997-03-26 1998-10-01 Rpms Technology Limited Proteines hybrides anticoagulantes ancrees dans des membranes cellulaires

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0256421A2 (fr) * 1986-08-12 1988-02-24 Phillips Petroleum Company Sécrétion de protéines hétérologues à partir de la levure
WO1989000205A1 (fr) * 1987-07-02 1989-01-12 American National Red Cross Nouvel activateur de proteine c
EP0323722A1 (fr) * 1987-12-18 1989-07-12 Chiron Corporation Compositions et méthodes de production de la fibrolase de crotalidus venom par ADN recombinant
WO1998042850A1 (fr) * 1997-03-26 1998-10-01 Rpms Technology Limited Proteines hybrides anticoagulantes ancrees dans des membranes cellulaires

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CANADIAN JOURNAL OF BOTANY, vol. 73, no. SUPPL. 1 SECT. E-H, 1995, pages S891 - S897, ISSN: 0008-4026 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, CREGG JAMES M ET AL: "Production of foreign proteins in the yeast Pichia pastoris.", XP002145444, Database accession no. PREV199699198650 *
MCMULLEN B A ET AL: "PRIMARY STRUCTURE OF A PROTEIN C ACTIVATOR FROM AGKISTRODON-CONTORTRIX-CONTORTRIX VENOM", BIOCHEMISTRY, vol. 28, no. 2, 1989, pages 674 - 679, XP002145442, ISSN: 0006-2960 *
NAKAGAKI T ET AL: "ISOLATION AND CHARACTERIZATION OF A PROTEIN C ACTIVATOR FROM TROPICAL MOCCASIN VENOM", THROMBOSIS RESEARCH, vol. 58, no. 6, 1990, pages 593 - 602, XP000093850, ISSN: 0049-3848 *
ZHANG YUN ET AL: "A Novel Plasminogen Activator from Snake Venom: Purification, characterization and molecular cloning.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 17, 1995, pages 10246 - 10255, XP002145443, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU3703200A (en) 2000-09-14
WO2000050612A2 (fr) 2000-08-31

Similar Documents

Publication Publication Date Title
EP2189475A3 (fr) Polypeptides de type facteur de croissance des fibroblastes
WO1999053061A3 (fr) Acides nucleiques associes a des tumeurs et leur emploi
WO2001072957A3 (fr) Molecules de type facteur de croissance des fibroblastes et leurs utilisations
WO2003066842A3 (fr) Procede pour produire des proteines de recombinaison dans des micro-organismes
WO2002008277A3 (fr) Acides nucleiques, vecteurs, cellules hotes, polypeptides et leurs utilisations
WO2000006726A3 (fr) Polypeptides relatifs a delta
WO2002012899A3 (fr) Peptides presents par cellules
WO2003008449A8 (fr) Ntb-a, une molecule de surface impliquee dans l'activite des cellules tueuses naturelles
WO2002077241A3 (fr) Proteines metalloproteases humaines isolees, molecules d'acide nucleique codant ces proteines proteases et utilisations de ces dernieres
WO2000050612A3 (fr) Activateur de proteine c recombinant et ses utilisations
DE602004009292D1 (de) Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
WO2004065554A3 (fr) Molecule d'acide nucleique humaine saa3, proteine, et procedes d'utilisation
WO2002008424A1 (fr) Genes abca2 humain et de rat
WO2004035733A3 (fr) Proteines hepsine/46 humaines isolees, molecules d'acide nucleique codant les proteines hepsine/46, et utilisations associees
WO2002046408A3 (fr) Nouvelles proteines humaines, polynucleotides les codant et procedes d'utilisation
WO1999031256A3 (fr) Proteines cathepsine dc, adn codant pour ces proteines et utilisation de ces proteines dans l'etablissement de pronostics pour les cancers chez l'homme
WO1999055885A3 (fr) Nouvelle serine protease apte au clivage selectif de la proteine de liaison du facteur de croissance insuline-like
WO2002018536A3 (fr) Proteine bard1 tronquee, et ses applications diagnostiques et therapeutiques
WO2005049818A3 (fr) Gene
WO2003012120A3 (fr) Proteine secretee de mammifere lp354
WO2002081629A3 (fr) Nouvelles proteines humaines, polynucleotides codant celles-ci et procede d'utilisation de ceux-ci
WO2002063009A3 (fr) Proteines de mammiferes lp et reactifs associes
WO2001012788A3 (fr) Polypeptide ztryp1 de type tryptase
WO2001062786A3 (fr) Peptides tlp et sequences d'adn codant pour eux

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase