WO2000049031A3 - Procede d'isolement d'acides nucleiques - Google Patents

Procede d'isolement d'acides nucleiques Download PDF

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Publication number
WO2000049031A3
WO2000049031A3 PCT/EP2000/001028 EP0001028W WO0049031A3 WO 2000049031 A3 WO2000049031 A3 WO 2000049031A3 EP 0001028 W EP0001028 W EP 0001028W WO 0049031 A3 WO0049031 A3 WO 0049031A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
isolating nucleic
acids
liberated
acidic
Prior art date
Application number
PCT/EP2000/001028
Other languages
German (de)
English (en)
Other versions
WO2000049031A2 (fr
Inventor
Wolfgang Podszun
Rainer Neumann
Original Assignee
Bayer Ag
Wolfgang Podszun
Rainer Neumann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag, Wolfgang Podszun, Rainer Neumann filed Critical Bayer Ag
Priority to CA002362979A priority Critical patent/CA2362979A1/fr
Priority to AU25471/00A priority patent/AU2547100A/en
Priority to EP00903676A priority patent/EP1155026A2/fr
Priority to JP2000599768A priority patent/JP2002537306A/ja
Publication of WO2000049031A2 publication Critical patent/WO2000049031A2/fr
Publication of WO2000049031A3 publication Critical patent/WO2000049031A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Cette invention concerne des acides nucléiques pouvant être efficacement adsorbés dans un milieu neutre ou acide sur des polymères en perles spécifiques qui sont insolubles dans l'eau et dont la taille moyenne des particules se situe entre 3 et 100 νm. Dans un milieu basique, ces acides sont libérés avec un débit plus important.
PCT/EP2000/001028 1999-02-19 2000-02-09 Procede d'isolement d'acides nucleiques WO2000049031A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002362979A CA2362979A1 (fr) 1999-02-19 2000-02-09 Procede d'isolement d'acides nucleiques
AU25471/00A AU2547100A (en) 1999-02-19 2000-02-09 Method for isolating nucleic acids
EP00903676A EP1155026A2 (fr) 1999-02-19 2000-02-09 Procede d'isolement d'acides nucleiques
JP2000599768A JP2002537306A (ja) 1999-02-19 2000-02-09 核酸を単離する方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19907023A DE19907023A1 (de) 1999-02-19 1999-02-19 Verfahren zur Isolierung von Nucleinsäuren
DE19907023.7 1999-02-19

Publications (2)

Publication Number Publication Date
WO2000049031A2 WO2000049031A2 (fr) 2000-08-24
WO2000049031A3 true WO2000049031A3 (fr) 2001-04-12

Family

ID=7898058

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/001028 WO2000049031A2 (fr) 1999-02-19 2000-02-09 Procede d'isolement d'acides nucleiques

Country Status (6)

Country Link
EP (1) EP1155026A2 (fr)
JP (1) JP2002537306A (fr)
AU (1) AU2547100A (fr)
CA (1) CA2362979A1 (fr)
DE (1) DE19907023A1 (fr)
WO (1) WO2000049031A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE10261910A1 (de) * 2002-12-30 2004-07-15 Polymerics Gmbh Adsorbermaterial für Blut-, Blutplasma- und Albuminreinigungsverfahren
US7098253B2 (en) 2004-05-20 2006-08-29 3M Innovative Properties Company Macroporous ion exchange resins
US7683100B2 (en) 2005-12-21 2010-03-23 3M Innovative Properties Company Method of making macroporous cation exchange resins
US7674835B2 (en) 2005-12-21 2010-03-09 3M Innovative Properties Company Method of making macroporous anion exchange resins
US7674836B2 (en) 2006-07-28 2010-03-09 3M Innovative Properties Company Method of making macroporous cation exchange resins
DE102008063003A1 (de) 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
CN108431129B (zh) * 2015-12-25 2021-10-08 株式会社可乐丽 水性乳液和使用其得到的粘接剂

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4839231A (en) * 1985-01-10 1989-06-13 Plant Genetic Systems N.V. Agents and procedures for the transfer of proteins and/or nucleic acids onto a supported receptor surface
WO1991005606A1 (fr) * 1989-10-21 1991-05-02 Macherey-Nagel & Co. Materiau de support et son utilisation dans un procede de separation chromatographique d'acides nucleiques
EP0572115A2 (fr) * 1992-05-29 1993-12-01 Rohm And Haas Company Copolymères réticulés d'anhydride d'acide méthacrylique
WO1997008547A1 (fr) * 1995-08-24 1997-03-06 Theobald Smith Research Institute, Inc. Procede et appareil pour isoler un acide nucleique
WO1997034909A1 (fr) * 1996-03-20 1997-09-25 Bio Merieux Isolement de l'acide nucleique

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4839231A (en) * 1985-01-10 1989-06-13 Plant Genetic Systems N.V. Agents and procedures for the transfer of proteins and/or nucleic acids onto a supported receptor surface
WO1991005606A1 (fr) * 1989-10-21 1991-05-02 Macherey-Nagel & Co. Materiau de support et son utilisation dans un procede de separation chromatographique d'acides nucleiques
EP0572115A2 (fr) * 1992-05-29 1993-12-01 Rohm And Haas Company Copolymères réticulés d'anhydride d'acide méthacrylique
WO1997008547A1 (fr) * 1995-08-24 1997-03-06 Theobald Smith Research Institute, Inc. Procede et appareil pour isoler un acide nucleique
WO1997034909A1 (fr) * 1996-03-20 1997-09-25 Bio Merieux Isolement de l'acide nucleique

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691969B2 (en) 1994-12-12 2014-04-08 Life Technologies As Isolation of nucleic acid

Also Published As

Publication number Publication date
JP2002537306A (ja) 2002-11-05
AU2547100A (en) 2000-09-04
WO2000049031A2 (fr) 2000-08-24
DE19907023A1 (de) 2000-08-24
EP1155026A2 (fr) 2001-11-21
CA2362979A1 (fr) 2000-08-24

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