WO2000047598A1 - Method and apparatus for compound library preparation using optical modulator - Google Patents

Method and apparatus for compound library preparation using optical modulator Download PDF

Info

Publication number
WO2000047598A1
WO2000047598A1 PCT/KR2000/000100 KR0000100W WO0047598A1 WO 2000047598 A1 WO2000047598 A1 WO 2000047598A1 KR 0000100 W KR0000100 W KR 0000100W WO 0047598 A1 WO0047598 A1 WO 0047598A1
Authority
WO
WIPO (PCT)
Prior art keywords
micro
compound
pluralities
wells
light
Prior art date
Application number
PCT/KR2000/000100
Other languages
English (en)
French (fr)
Inventor
Jeong-Sun Seo
Yoon-Sik Lee
Yong-Kweon Kim
Original Assignee
Macrogen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Macrogen Inc. filed Critical Macrogen Inc.
Priority to EP00904105A priority Critical patent/EP1180111A4/en
Priority to JP2000598516A priority patent/JP2002541433A/ja
Priority to AU25777/00A priority patent/AU2577700A/en
Publication of WO2000047598A1 publication Critical patent/WO2000047598A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00436—Maskless processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00436—Maskless processes
    • B01J2219/00439—Maskless processes using micromirror arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Definitions

  • the present invention relates to a providing of a compound library, more particularly to a providing of a compound library preparation method using an optical modulator and a compound library preparation apparatus. Furthermore, the present invention provides a compound analyzing method
  • desired compounds are synthesized are bonded to the functional groups, and then light is irradiated only on the necessary area thereof so that the functional groups are exposed.
  • a compound library is synthesized.
  • the present invention provides a method and apparatus for effectively preparing varieties of compounds in a simple process without
  • the present invention seeks to decrease error occurrence that is
  • the present invention easily provides various compound libraries by
  • the present invention provides a method for the preparation of a
  • optical modulator is of a construction in which pluralities of
  • optical switches are aligned, and each optical switch is a device
  • micro-well or moves or rotates an opaque masking plate to
  • step b) can be substituted with a step
  • the method of the present invention can be applied to a variety of
  • the present invention provides compound library chips
  • the present invention provides an apparatus for the preparation of
  • micro-wells that are not to be bonded; and e) a controller comprising at least one optical switch address, irradiating light on a micro-well according to the time, order of compounds to be injected, and information on final compounds produced on each micro-well, and having programs controlling the operating order of the optical irradiator, the compound injecting device, and the washing device.
  • Typical configurations of an optical modulator include the following
  • the first example is an optical modulator having a separate medium
  • a substrate plate which supports the micro-mirror, aligned on a plane, placed separately from the micro-mirror, and comprising
  • each micro-mirror, a landing electrode, and the micro-mirror is a micro-mirror, a landing electrode, and the micro-mirror
  • optical modulator comprising:
  • Another example is an optical modulator irradiating light only at the
  • desired micro-wells comprising:
  • the present invention provides a compound analyzing
  • the present invention provides a compound analyzing
  • FIG. 1 apparatus (FIG. 1 ) comprising:
  • Exemplary compounds of the present invention include varieties of
  • wall as used in the present invention not only refers to the meaning usually used in the field of the present invention but also as a
  • FIG. 2 consists of pluralities of micro-mirrors (a) on a substrate plate (b) (FIG. 2),
  • micro-mirrors are individually adjusted with electrical signals.
  • Each micro-mirror can irradiate light only on a particular micro-well of the substrate plate, which comprises corresponding pluralities of micro-wells of the same magnitude, so that functional groups are activated only at the desired position. Furthermore, other libraries can be obtained depending on the quantity of light irradiated since even the same method can control the reflecting time of a micro-mirror.
  • the present invention is based on an idea that various types of biochips can be effectively manufactured if the micro-mirror is used instead of a photomask as in the previous compound
  • An optical modulator can two-dimensionally align a micro-mirror (a)
  • Each micro-mirror has rotation angles of at least
  • FIG. 4 a illustrates a micro-well
  • micro-mirror c illustrates the incident light
  • d illustrates the reflected light
  • optical modulator The embodiment examples of an optical modulator are as follows.
  • micro-mirror a is connected to pillar c by torsion spring b, wherein pillar c is fixed to substrate plate d as shown in FIG.
  • Micro-mirror a is tilted by applying voltage to one side of electrode e,
  • micro-mirror b is manufactured
  • micro-mirror b can be driven by cladding piezoelectric material or bimetal over cantilever a, as shown in FIG. 6.
  • a is a micro-mirror
  • b is an address electrode
  • c is a landing electrode
  • d is a hinge supporting post
  • e is an electrode supporting post.
  • f1 and f2 illustrate the states of tilt when a voltage is applied to an electrode.
  • Korean Patent Application No. 96-20611 can be referred to as the fourth embodiment example.
  • a prescribed patterned electrode (b) is formed
  • the electrode (b) has a strip shape
  • a post (f) is formed on the substrate
  • a micro-mirror is rotationally connected to the
  • a prescribed perforative slot (g) is formed on the top surface of a micro-mirror, and the post-integrated perforative slot is formed.
  • light can be irradiated through a
  • FIG. 9 and FIG. 10 is a state in which light of two windows is masked, and (b) is a state in which light of the right side windows is irradiated.
  • a protecting group of the present invention means a material that is
  • protecting groups include ⁇ -methyl-2-
  • Examples of an activator of the present invention preferably include
  • the controller program is an important component in the apparatus
  • This program should at least include: the types of compounds injected;
  • optical switch through a code which identifies each optical switch; the time of
  • additional apparatus includes a circuit that deciphers a controller code.
  • the prepared compound libraries are used to measure the activity of
  • the measuring example comprises the steps of i )
  • FIG. 1 illustrates a compound library preparation apparatus and a
  • FIG. 2 is an optical modulator in which pluralities of micro-mirrors
  • optical switch are configured by an optical switch
  • FIG. 3 illustrates a state in which light is reflected on a micro-well
  • FIG. 4 illustrates a state in which a micro-mirror does not reflect light
  • FIG. 5 to FIG. 10 are embodiment examples of an optical modulator
  • FIG. 1 1 (a) and FIG. 1 1 (b) are front and side scanning electron
  • FIG. 12 illustrates a direction in which a mirror array pixel bloc is
  • FIG. 13 is a microscope photograph in which a practically driven
  • FIG. 14 illustrates a procedure in which polynucleotide library is
  • FIG. 15 illustrates a procedure in which a polypeptide library is
  • the invention is capable of modification in
  • FIG. 12 illustrates a direction in which a mirror array pixel bloc is
  • FIG. 13 is a microscope photograph in which the practically
  • the floor of the micro-well was washed with the chloroform solution and dried.
  • reaction termination can be confirmed when the color is removed.
  • nucleotide used in the reaction can be easily combined with an amino group on a chip due to its carboxylic acid shape, and the 5' position has a
  • Ultraviolet rays of 360 nm were irradiated through a micro-mirror for 15 minutes, removing a protecting group at a desired position. After washing
  • nucleotide used in the reaction can be easily combined with the 5' position
  • nucleotide has a protecting group which is decomposed by light (Chemical Formula 4).
  • FIG. 14 represents a process in which
  • polynucleotide C-G-T-A and A-G-T-C are prepared through the above
  • a process for synthesizing a peptide library on a chip is similar to the foregoing.
  • a compound library chip employing a protecting group at a terminal end amine group was mounted on a system and washed with dried acetonitrile.
  • a protecting group was selectively removed and washed with a dried NMP solution. It was reacted for 60 minutes by injecting the already prepared 0.1 M amino acid/ NMP solution and 0.5 M BOP/ HOBt/NMP solution into a micro-well. After the coupling reaction, it was washed with the NMP solution for 10 minutes.
  • Each amino acid used in the coupling reaction has a protecting group of
  • FIG. 15 represents a process in which
  • polypeptide G-E-C-A and H-F-D-B are synthesized through the above
  • the present invention has the following merits because of the use of an optical modulator instead of conventionally used photomasks.
  • an optical switch can be individually operated.
  • an automation of the compound library preparation process can be more easily accomplished since the optical irradiation position and quantity of light can be simply controlled by an electrical signal of a controller. Furthermore, it is appropriate to compose only the program itself even though other configurations are required since the compound library
  • compound composition can be easily modified by controlling the quantity of
PCT/KR2000/000100 1999-02-10 2000-02-10 Method and apparatus for compound library preparation using optical modulator WO2000047598A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00904105A EP1180111A4 (en) 1999-02-10 2000-02-10 METHOD AND DEVICE FOR PRODUCING CONNECTION LIBRARIES BY MEANS OF OPTICAL MODULATOR
JP2000598516A JP2002541433A (ja) 1999-02-10 2000-02-10 光変調器を利用した化学物質ライブラリー製造方法及び製造装置
AU25777/00A AU2577700A (en) 1999-02-10 2000-02-10 Method and apparatus for compound library preparation using optical modulator

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR19990004639 1999-02-10
KR1999/4639 1999-02-10

Publications (1)

Publication Number Publication Date
WO2000047598A1 true WO2000047598A1 (en) 2000-08-17

Family

ID=19573988

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2000/000100 WO2000047598A1 (en) 1999-02-10 2000-02-10 Method and apparatus for compound library preparation using optical modulator

Country Status (4)

Country Link
EP (1) EP1180111A4 (ja)
JP (1) JP2002541433A (ja)
AU (1) AU2577700A (ja)
WO (1) WO2000047598A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0961174A2 (en) * 1998-05-29 1999-12-01 Affymetrix, Inc. (a California Corporation) Compositions and methods involving direct write optical lithography
WO2004065635A2 (en) * 2002-12-16 2004-08-05 Intel Corporation Laser exposure of photosensitive masks for dna microarray fabrication
US7544638B2 (en) 1998-02-11 2009-06-09 The Regents Of The University Of Michigan Device for chemical and biochemical reactions using photo-generated reagents

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007254542A (ja) * 2006-03-22 2007-10-04 Oki Electric Ind Co Ltd 有機化合物合成装置および有機化合物合成用基板

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5258506A (en) * 1984-10-16 1993-11-02 Chiron Corporation Photolabile reagents for incorporation into oligonucleotide chains
JPH07306368A (ja) * 1994-05-13 1995-11-21 Sony Corp デジタルマイクロミラーデバイス
JPH09159937A (ja) * 1995-12-01 1997-06-20 Seiko Epson Corp 光変調装置及びその製造方法並びにその光変調装置を用いた電子機器
JPH09236762A (ja) * 1996-02-29 1997-09-09 Seiko Epson Corp 空間光変調器およびその製造方法、並びにその空間光変調器を用いた電子機器

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424186A (en) * 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US6219015B1 (en) * 1992-04-28 2001-04-17 The Board Of Directors Of The Leland Stanford, Junior University Method and apparatus for using an array of grating light valves to produce multicolor optical images
US5545531A (en) * 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
US6426184B1 (en) * 1998-02-11 2002-07-30 The Regents Of The University Of Michigan Method and apparatus for chemical and biochemical reactions using photo-generated reagents
JP4698023B2 (ja) * 1998-02-23 2011-06-08 ウイスコンシン アラムニ リサーチ ファンデーション Ｄｎａプローブのアレイの合成方法及び合成装置
US6271957B1 (en) * 1998-05-29 2001-08-07 Affymetrix, Inc. Methods involving direct write optical lithography
WO1999063385A1 (en) * 1998-06-04 1999-12-09 Board Of Regents, The University Of Texas System Digital optical chemistry micromirror imager

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258506A (en) * 1984-10-16 1993-11-02 Chiron Corporation Photolabile reagents for incorporation into oligonucleotide chains
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH07306368A (ja) * 1994-05-13 1995-11-21 Sony Corp デジタルマイクロミラーデバイス
JPH09159937A (ja) * 1995-12-01 1997-06-20 Seiko Epson Corp 光変調装置及びその製造方法並びにその光変調装置を用いた電子機器
JPH09236762A (ja) * 1996-02-29 1997-09-09 Seiko Epson Corp 空間光変調器およびその製造方法、並びにその空間光変調器を用いた電子機器

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1180111A4 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7544638B2 (en) 1998-02-11 2009-06-09 The Regents Of The University Of Michigan Device for chemical and biochemical reactions using photo-generated reagents
US7838466B2 (en) 1998-02-11 2010-11-23 The Regents Of The University Of Michigan Device for chemical and biochemical reactions using photo-generated reagents
EP0961174A2 (en) * 1998-05-29 1999-12-01 Affymetrix, Inc. (a California Corporation) Compositions and methods involving direct write optical lithography
EP0961174A3 (en) * 1998-05-29 2001-01-17 Affymetrix, Inc. (a California Corporation) Compositions and methods involving direct write optical lithography
US6480324B2 (en) 1998-05-29 2002-11-12 Affymetrix, Inc. Methods involving direct write optical lithography
WO2004065635A2 (en) * 2002-12-16 2004-08-05 Intel Corporation Laser exposure of photosensitive masks for dna microarray fabrication
WO2004065635A3 (en) * 2002-12-16 2005-02-03 Intel Corp Laser exposure of photosensitive masks for dna microarray fabrication

Also Published As

Publication number Publication date
EP1180111A4 (en) 2003-03-26
JP2002541433A (ja) 2002-12-03
AU2577700A (en) 2000-08-29
EP1180111A1 (en) 2002-02-20

Similar Documents

Publication Publication Date Title
US6261776B1 (en) Nucleic acid arrays
US6406844B1 (en) Very large scale immobilized polymer synthesis
US6506558B1 (en) Very large scale immobilized polymer synthesis
US5424186A (en) Very large scale immobilized polymer synthesis
US6936472B2 (en) Method for synthesizing a specific, surface-bound polymer uniformly over an element of a molecular array
JP4740020B2 (ja) 物質の電気化学的配置方法
DE69233331T3 (de) Kombinatorische Strategien zur Polymersynthese
US20020137096A1 (en) Apparatus comprising polymers
EP1180111A1 (en) Method and apparatus for compound library preparation using optical modulator
US20070224629A1 (en) Laser exposure of photosensitive masks for dna microarray fabrication
KR100497673B1 (ko) 광 변조기를 이용한 화학물질 라이브러리 제조 방법 및제조 장치

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AU BA BB BG BR CA CN CU CZ EE GD GE HR HU ID IL IN IS JP KP LC LK LR LT LV MG MK MN MX NO NZ PL RO SG SI SK SL TR TT UA US UZ VN YU

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 598516

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000904105

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000904105

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2000904105

Country of ref document: EP