WO2000046383A3 - Method of modulating metabolite biosynthesis in recombinant cells - Google Patents

Method of modulating metabolite biosynthesis in recombinant cells Download PDF

Info

Publication number
WO2000046383A3
WO2000046383A3 PCT/NL2000/000075 NL0000075W WO0046383A3 WO 2000046383 A3 WO2000046383 A3 WO 2000046383A3 NL 0000075 W NL0000075 W NL 0000075W WO 0046383 A3 WO0046383 A3 WO 0046383A3
Authority
WO
WIPO (PCT)
Prior art keywords
modulating
cell
biosynthesis
metabolite
recombinant cells
Prior art date
Application number
PCT/NL2000/000075
Other languages
French (fr)
Other versions
WO2000046383A2 (en
WO2000046383A8 (en
Inventor
Johan Memelink
Der Fits Cornelia Theodora Van
Frank Leonardus Hendriku Menke
Jan Willem Kijne
Original Assignee
Univ Leiden
Johan Memelink
Fits Cornelia Theodora Elisabe
Frank Leonardus Hendriku Menke
Jan Willem Kijne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leiden, Johan Memelink, Fits Cornelia Theodora Elisabe, Frank Leonardus Hendriku Menke, Jan Willem Kijne filed Critical Univ Leiden
Priority to US09/890,782 priority Critical patent/US7393946B1/en
Priority to NZ513356A priority patent/NZ513356A/en
Priority to JP2000597442A priority patent/JP2002535993A/en
Priority to EP00904136A priority patent/EP1157119A1/en
Priority to CA002361678A priority patent/CA2361678A1/en
Priority to AU25806/00A priority patent/AU779162B2/en
Publication of WO2000046383A2 publication Critical patent/WO2000046383A2/en
Publication of WO2000046383A3 publication Critical patent/WO2000046383A3/en
Publication of WO2000046383A8 publication Critical patent/WO2000046383A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A method for modulating in a cell the expression of one or more genes involved in the biosynthesis of a metabolite or a precursor for this metabolite by inserting into a cell a nucleotide sequence coding for a transcription factor comprising an AP2 DNA-binding domain, and/or by modifying the expression of a nucleotide sequence coding for such a transcription factor already present in the cell is provided. The method is useful for enhancing the biosynthesis of secondary metabolites in plants such as alkaloids including terpenoid indole alkaloids. Also provided is a method for enhancing stress tolerance in plants by the use of such transcription factors.
PCT/NL2000/000075 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells WO2000046383A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US09/890,782 US7393946B1 (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells
NZ513356A NZ513356A (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells using an AP2-domain transcription factor that is involved in the response of a plant cell to a jasmonate
JP2000597442A JP2002535993A (en) 1999-02-05 2000-02-07 Methods for modulating biosynthesis of metabolites in recombinant cells
EP00904136A EP1157119A1 (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells
CA002361678A CA2361678A1 (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells
AU25806/00A AU779162B2 (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA199900158 1999-02-05
DKPA199900158 1999-02-05
US11938899P 1999-02-10 1999-02-10
US60/119,388 1999-02-10

Publications (3)

Publication Number Publication Date
WO2000046383A2 WO2000046383A2 (en) 2000-08-10
WO2000046383A3 true WO2000046383A3 (en) 2001-09-07
WO2000046383A8 WO2000046383A8 (en) 2001-11-01

Family

ID=26063424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2000/000075 WO2000046383A2 (en) 1999-02-05 2000-02-07 Method of modulating metabolite biosynthesis in recombinant cells

Country Status (6)

Country Link
EP (1) EP1157119A1 (en)
JP (1) JP2002535993A (en)
AU (1) AU779162B2 (en)
CA (1) CA2361678A1 (en)
NZ (1) NZ513356A (en)
WO (1) WO2000046383A2 (en)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7888558B2 (en) 1999-11-17 2011-02-15 Mendel Biotechnology, Inc. Conferring biotic and abiotic stress tolerance in plants
US8912394B2 (en) 2001-04-18 2014-12-16 Mendel Biotechnology Inc. Transcriptional regulation of plant disease tolerance
US7663025B2 (en) 1999-03-23 2010-02-16 Mendel Biotechnology, Inc. Plant Transcriptional Regulators
US8030546B2 (en) 1998-09-22 2011-10-04 Mendel Biotechnology, Inc. Biotic and abiotic stress tolerance in plants
US7858848B2 (en) 1999-11-17 2010-12-28 Mendel Biotechnology Inc. Transcription factors for increasing yield
US6835540B2 (en) * 2001-03-16 2004-12-28 Mendel Biotechnology, Inc. Biosynthetic pathway transcription factors
WO2000070059A2 (en) * 1999-05-14 2000-11-23 Pioneer Hi-Bred International, Inc. Signal transduction genes and methods of use
WO2002015675A1 (en) * 2000-08-22 2002-02-28 Mendel Biotechnology, Inc. Genes for modifying plant traits iv
CA2444482A1 (en) 2001-04-18 2002-10-24 Alain Goossens The use of genes encoding abc transporters to stimulate the production of secondary metabolites in biological cells
EP1293572A1 (en) * 2001-09-17 2003-03-19 Syngenta Participations AG Method of altering plant secondary metobolite biosynthesis in plant cells
US7176027B2 (en) 2001-12-20 2007-02-13 Pioneer Hi-Bred International, Inc. Genes and regulatory DNA sequences associated with stress-related gene expression in plants and methods of using the same
WO2003059928A1 (en) * 2002-01-15 2003-07-24 Avestha Gengraine Technologies Pvt. Ltd. Polynucleotide encoding an ap-2 domain transcription factor
CN104383542B (en) 2003-04-08 2017-09-26 普罗热尼奇制药公司 Pharmaceutical formulation comprising methyl naltrexone
EP1844073A1 (en) 2005-01-31 2007-10-17 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
WO2006115575A1 (en) 2005-04-20 2006-11-02 Ceres Inc. Regulatory regions from papaveraceae
ATE537188T1 (en) 2005-05-18 2011-12-15 Ablynx Nv SERUM ALBUM BINDING PROTEINS
SI2444424T1 (en) 2005-05-20 2018-10-30 Ablynx N.V. Improved nanobodies tm for the treatment of aggregation-mediated disorders
WO2008074840A2 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against a metalloproteinase from the adam family and polypeptides comprising the same for the treatment of adam-related diseases and disorders
WO2008074839A2 (en) 2006-12-19 2008-06-26 Ablynx N.V. Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders
MX351611B (en) 2007-03-29 2017-10-20 Wyeth Llc Crystal forms of (r) -n-methylnaltrexone bromide and uses thereof.
AU2008328785A1 (en) 2007-11-27 2009-06-04 Ablynx N.V. Method for obtaining polypeptide constructs comprising two or more single domain antibodies
EP2730578A1 (en) 2008-02-06 2014-05-14 Progenics Pharmaceuticals, Inc. Preparation and use of (r),(r)-2,2'-bis-methylnal trexone
CN101965362A (en) 2008-03-05 2011-02-02 埃博灵克斯股份有限公司 Novel antigens is in conjunction with dimer-mixture and its production and application
JP2011516520A (en) 2008-04-07 2011-05-26 アブリンクス エン．ヴェー． Amino acid sequence having directivity in Notch pathway and use thereof
WO2009127691A1 (en) 2008-04-17 2009-10-22 Ablynx N.V. Peptides capable of binding to serum proteins and compounds, constructs and polypeptides comprising the same
AU2009255796B2 (en) * 2008-06-06 2014-09-25 Grasslanz Technology Limited Novel genes involved in biosynthesis
EP2403873A1 (en) 2009-03-05 2012-01-11 Ablynx N.V. Novel antigen binding dimer-complexes, methods of making/avoiding and uses thereof
EP2417163B1 (en) 2009-04-10 2019-02-27 Ablynx N.V. Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders
HUE035773T2 (en) 2009-06-05 2018-05-28 Ablynx Nv Trivalent anti human respiratory syncytial virus (hrsv) nanobody constructs for the prevention and/or treatment of respiratory tract infections
WO2011040978A2 (en) 2009-10-02 2011-04-07 Ludwig Institute For Cancer Research Ltd. Immunodominant mhc dr52b restricted ny-eso-1 epitopes, mhc class ii monomers and multimers, and uses thereof
WO2011064382A1 (en) 2009-11-30 2011-06-03 Ablynx N.V. Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
CA2788993A1 (en) 2010-02-05 2011-08-11 Ablynx N.V. Peptides capable of binding to serum albumin and compounds, constructs and polypeptides comprising the same
TWI619811B (en) 2010-11-08 2018-04-01 諾華公司 Chemokine receptor binding polypeptides
EP2718309B1 (en) 2011-06-10 2018-04-04 Keygene N.V. Transcription factor modulating terpene biosynthesis
AU2012273928A1 (en) 2011-06-23 2014-02-06 Ablynx Nv Immunoglobulin single variable domains directed against IgE
US9328174B2 (en) 2012-05-09 2016-05-03 Novartis Ag Chemokine receptor binding polypeptides
WO2014087010A1 (en) 2012-12-07 2014-06-12 Ablynx N.V. IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE
NL1040254C2 (en) 2013-05-17 2014-11-24 Ablynx Nv Stable formulations of immunoglobulin single variable domains and uses thereof.
NL2013007B1 (en) 2014-06-16 2016-07-05 Ablynx Nv Methods of treating TTP with immunoglobulin single variable domains and uses thereof.
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
KR20230132604A (en) 2015-01-27 2023-09-15 라바 테라퓨틱스 엔.브이. Single domain antibodies targeting cd1d
AU2016360987A1 (en) 2015-11-27 2018-06-07 Ablynx Nv Polypeptides inhibiting CD40L
EP3512880A1 (en) 2016-09-15 2019-07-24 Ablynx NV Immunoglobulin single variable domains directed against macrophage migration inhibitory factor
BR112019010061A2 (en) 2016-11-16 2019-08-13 Ablynx Nv t-cell recruitment polypeptides capable of binding to cd123 and tcr alpha / beta
KR20200012935A (en) 2017-06-02 2020-02-05 메르크 파텐트 게엠베하 ADAMTS binding immunoglobulins
TW202417517A (en) 2017-06-02 2024-05-01 德商麥克專利有限公司 Mmp13 binding immunoglobulins
WO2018220225A1 (en) 2017-06-02 2018-12-06 Ablynx Nv Aggrecan binding immunoglobulins
EP3630817A1 (en) 2017-06-02 2020-04-08 Merck Patent GmbH Polypeptides binding adamts5, mmp13 and aggrecan
MX2020008294A (en) 2018-02-06 2020-11-18 Ablynx Nv Methods of treating initial episode of ttp with immunoglobulin single variable domains.
EP4378485A3 (en) 2019-03-08 2024-08-28 LinXis B.V. Internalizing binding molecules targeting receptors involved in cell proliferation or cell differentiation
WO2020245663A1 (en) 2019-06-01 2020-12-10 Institut Pasteur Nanobody-based ns1 assay for the specific diagnosis of acute zika virus infection
MX2022001491A (en) * 2019-08-05 2022-04-20 Univ Virginia Patent Foundation TRANSCRIPTION FACTOR NtERF221 AND METHODS OF USING THE SAME.
CN110526961B (en) * 2019-09-09 2021-02-19 四川大学 Application of transcription factor participating in regulation and control of synthesis of tomato steroid alkaloid
CN113265388B (en) * 2020-02-14 2023-02-03 中国科学院分子植物科学卓越创新中心 Tobacco system for producing ajmaline
EP4114942A4 (en) * 2020-03-04 2024-08-21 Pioneer Hi Bred Int Cis-acting regulatory elements
CN111690661A (en) * 2020-06-01 2020-09-22 云南省烟草农业科学研究院 Tobacco NtbHLH13 gene mutant and molecular identification method and application
JP2023541934A (en) 2020-09-16 2023-10-04 リンクシス ベスローテン フェンノートシャップ internalized binding molecules
EP4019548A1 (en) 2020-12-23 2022-06-29 Vrije Universiteit Brussel Anti lag3 vhhs and their use
CN115433733A (en) 2021-06-04 2022-12-06 生物岛实验室 Cyclic RNA (ribonucleic acid) Circ-ACE2 translated polypeptide and application thereof
WO2023068382A2 (en) 2021-10-20 2023-04-27 Takeda Pharmaceutical Company Limited Compositions targeting bcma and methods of use thereof
US20240132624A1 (en) 2022-07-27 2024-04-25 Ablynx N.V. Polypeptides binding to a specific epitope of the neonatal fc receptor
WO2024133935A1 (en) 2022-12-23 2024-06-27 Ablynx Nv Protein-based conjugation carriers
WO2024170756A1 (en) 2023-02-17 2024-08-22 Ablynx N.V. Polypeptides binding to the neonatal fc receptor

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09224672A (en) * 1996-02-21 1997-09-02 Mitsui Gyosai Shokubutsu Bio Kenkyusho:Kk Dna coding new dna-connected protein
WO1998007842A1 (en) * 1996-08-20 1998-02-26 The Regents Of The University Of California Methods for improving seeds
WO1998009521A1 (en) * 1996-09-04 1998-03-12 Michigan State University Dna and encoded protein which regulates cold and dehydration regulated genes
WO1999037794A1 (en) * 1998-01-26 1999-07-29 Unilever Plc Methods and composition for modulating flavonoid content
WO1999038977A2 (en) * 1998-02-03 1999-08-05 Mendel Biotechnology, Inc. Plant having altered environmental stress tolerance
WO2000009712A1 (en) * 1998-08-11 2000-02-24 Japan As Represented By Director General Of National Institute Of Agrobiological Resources, Ministry Of Agricalture, Forestry And Fisheries Transcription factors regulating the expression of ethylene-inducible genes
JP2000060558A (en) * 1998-08-12 2000-02-29 Norin Suisansyo Kokusai Norin Suisangyo Kenkyu Center Shocho Gene encoding transcription factor of plant
JP2000116260A (en) * 1998-10-14 2000-04-25 Japan International Res Center For Agricultural Sciences Plant resistant to environmental stress

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09224672A (en) * 1996-02-21 1997-09-02 Mitsui Gyosai Shokubutsu Bio Kenkyusho:Kk Dna coding new dna-connected protein
WO1998007842A1 (en) * 1996-08-20 1998-02-26 The Regents Of The University Of California Methods for improving seeds
WO1998009521A1 (en) * 1996-09-04 1998-03-12 Michigan State University Dna and encoded protein which regulates cold and dehydration regulated genes
WO1999037794A1 (en) * 1998-01-26 1999-07-29 Unilever Plc Methods and composition for modulating flavonoid content
WO1999038977A2 (en) * 1998-02-03 1999-08-05 Mendel Biotechnology, Inc. Plant having altered environmental stress tolerance
WO2000009712A1 (en) * 1998-08-11 2000-02-24 Japan As Represented By Director General Of National Institute Of Agrobiological Resources, Ministry Of Agricalture, Forestry And Fisheries Transcription factors regulating the expression of ethylene-inducible genes
JP2000060558A (en) * 1998-08-12 2000-02-29 Norin Suisansyo Kokusai Norin Suisangyo Kenkyu Center Shocho Gene encoding transcription factor of plant
JP2000116260A (en) * 1998-10-14 2000-04-25 Japan International Res Center For Agricultural Sciences Plant resistant to environmental stress

Non-Patent Citations (18)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; July 1998 (1998-07-01), CANEL CAMILO ET AL: "Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus.", XP002145083, Database accession no. PREV199800351362 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; November 1997 (1997-11-01), HALLARD D ET AL: "Suspension cultured transgenic cells of Nicotiana tabacum expressing tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus produce strictosidine upon secologanin feeding.", XP002145094, Database accession no. PREV199800047837 *
DATABASE WPI Section Ch Week 199745, Derwent World Patents Index; Class B04, AN 1997-484101, XP002145098 *
DATABASE WPI Section Ch Week 200027, Derwent World Patents Index; Class C06, AN 2000-306709, XP002145084 *
DATABASE WPI Section Ch Week 200034, Derwent World Patents Index; Class C06, AN 2000-389819, XP002145085 *
GILMOUR S J ET AL: "Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold -induced COR gene expression", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 16, no. 4, November 1998 (1998-11-01), pages 433 - 442, XP002108959, ISSN: 0960-7412 *
GROTEWALD, E., ET AL.: "Engineering secondary metabolism in maize cells by ectopic expression of transcription factors", THE PLANT CELL, vol. 10, May 1998 (1998-05-01), pages 721 - 740, XP002145082 *
JAGLO-OTTOSEN KIRSTEN R ET AL: "Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance.", SCIENCE (WASHINGTON D C), vol. 280, no. 5360, 3 April 1998 (1998-04-03), pages 104 - 106, XP002145076, ISSN: 0036-8075 *
LIN, X., ET AL.: "Arabidopsis thaliana chromosome II section 240 of 255 of the complete sequence. Sequence from clones F16B22, T13E15.", EMBL ACCESSION NO:AC002388, 4 August 1997 (1997-08-04), XP002145135 *
LIU QIANG ET AL: "Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively, in Arabidopsis.", PLANT CELL, vol. 10, no. 8, August 1998 (1998-08-01), pages 1391 - 1406, XP002145075, ISSN: 1040-4651 *
MENKE FRANK L H ET AL: "A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 18, no. 16, 16 August 1999 (1999-08-16), pages 4455 - 4463, XP002145079, ISSN: 0261-4189 *
OHME-TAKAGI M.: "Nicotiana tabacum mRNA for ERF1, complete cds", EMBL ACCESSION NO:D38123, 1 May 1995 (1995-05-01), XP002145077 *
PASQUALI G ET AL: "COORDINATED REGULATION OF TWO INDOLE ALKALOID BIOSYNTHETIC GENES FROM CATHARANTHUS-ROSEUS BY AUXIN AND ELICITORS", PLANT MOLECULAR BIOLOGY, vol. 18, no. 6, 1992, pages 1121 - 1131, XP002145080, ISSN: 0167-4412 *
PASQUALI G.: "C.roseus str1 gene", EMBL ACCESSION NO:Y10182, 23 December 1996 (1996-12-23), XP002145078 *
PLANT CELL REPORTS, vol. 17, no. 1, November 1997 (1997-11-01), pages 50 - 54, ISSN: 0721-7714 *
PLANTA (BERLIN), vol. 205, no. 3, July 1998 (1998-07-01), pages 414 - 419, ISSN: 0032-0935 *
See also references of EP1157119A1 *
SHANKS JACQUELINE V ET AL: "Quantification of metabolites in the indole alkaloid pathways of Catharanthus roseus: Implications for metabolic engineering.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 58, no. 2-3, 20 April 1998 (1998-04-20), pages 333 - 338, XP002145081, ISSN: 0006-3592 *

Also Published As

Publication number Publication date
EP1157119A1 (en) 2001-11-28
WO2000046383A2 (en) 2000-08-10
AU2580600A (en) 2000-08-25
NZ513356A (en) 2004-01-30
CA2361678A1 (en) 2000-08-10
WO2000046383A8 (en) 2001-11-01
AU779162B2 (en) 2005-01-06
JP2002535993A (en) 2002-10-29

Similar Documents

Publication Publication Date Title
WO2000046383A3 (en) Method of modulating metabolite biosynthesis in recombinant cells
AU2831000A (en) Electrolytic membrane for fuel cell and its manufacturing method, and fuel cell and its manufacturing method
HK1063528A1 (en) Proton conducting membrane, method for producing the same, and fuel cell using the same
AU4500500A (en) Membranes, membrane electrode assemblies and fuel cells employing same, and process for preparing
AU7951400A (en) Method for producing electrode for lithium secondary cell
AU2001282569A1 (en) Negative electrode for lithium secondary cell and method for producing the same
AU5731400A (en) Methods of preparing electrochemical cells
AU1796601A (en) Methods of preparing electrochemical cells
WO2001090345A3 (en) Methods and compositions for enhancing the delivery of a nucleic acid to a cell
AU2001292539A1 (en) Fuel cell assembly and method for making the same
AU2001287683A1 (en) Bipolar plate for pem fuel cells
AU3171399A (en) Solid electrolyte fuel cell and method of producing the same
AU6878100A (en) Carbonaceous material for hydrogen storage and method for preparing the same, and cell and fuel cell
AU2003272877A1 (en) Electrode for fuel cell, fuel cell including the electrode and process for producing the same
AU2001261805A1 (en) Method for improving fuel cell performance
AU2002239439A1 (en) Catalyst inks and method of application for direct methanol fuel cells
AU3782101A (en) Production of pem fuel cell stacks
AU2003255035A1 (en) Method for testing precursor of secondary cell, its testing instrument, and method for manufacturing secondary cell using the method
AU2001290322A1 (en) Fuel cell and production method therefor
ZA200007458B (en) Method of preparing a fuel cell.
AU2003299661A1 (en) Fuel cell assemblies and methods of making the same
AU2001284481A1 (en) Separator for low-temperature type fuel cell and production method therefor
AU2001292318A1 (en) Fuel cell and production method therefor
AU1796501A (en) Methods of preparing electrochemical cells
WO2001072970A3 (en) Methods to prepare and use epidermal stem cells

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000904136

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 25806/00

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2361678

Country of ref document: CA

Ref country code: CA

Ref document number: 2361678

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 513356

Country of ref document: NZ

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 597442

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 09890776

Country of ref document: US

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: C1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i
WWP Wipo information: published in national office

Ref document number: 2000904136

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 09890782

Country of ref document: US

WWG Wipo information: grant in national office

Ref document number: 25806/00

Country of ref document: AU