WO2000017365A8 - Binary viral expression system in plants - Google Patents

Binary viral expression system in plants

Info

Publication number
WO2000017365A8
WO2000017365A8 PCT/US1999/021989 US9921989W WO0017365A8 WO 2000017365 A8 WO2000017365 A8 WO 2000017365A8 US 9921989 W US9921989 W US 9921989W WO 0017365 A8 WO0017365 A8 WO 0017365A8
Authority
WO
WIPO (PCT)
Prior art keywords
plants
expression system
viral expression
binary
binary viral
Prior art date
Application number
PCT/US1999/021989
Other languages
French (fr)
Other versions
WO2000017365A3 (en
WO2000017365A2 (en
Inventor
Narendra S Yadav
Original Assignee
Du Pont
Narendra S Yadav
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Narendra S Yadav filed Critical Du Pont
Priority to NZ510594A priority Critical patent/NZ510594A/en
Priority to AU14410/00A priority patent/AU1441000A/en
Priority to EP99969445A priority patent/EP1115870A2/en
Priority to CA002339595A priority patent/CA2339595A1/en
Priority to HU0104697A priority patent/HUP0104697A3/en
Publication of WO2000017365A2 publication Critical patent/WO2000017365A2/en
Publication of WO2000017365A3 publication Critical patent/WO2000017365A3/en
Publication of WO2000017365A8 publication Critical patent/WO2000017365A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to plant transgene expression systems comprising chromosomally-integrated components that are individually heritable. The systems are useful for the regulated expression of foreign genes through gene amplification in plant tissue.
PCT/US1999/021989 1998-09-23 1999-09-22 Binary viral expression system in plants WO2000017365A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
NZ510594A NZ510594A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
AU14410/00A AU1441000A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
EP99969445A EP1115870A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
CA002339595A CA2339595A1 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
HU0104697A HUP0104697A3 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10155898P 1998-09-23 1998-09-23
US60/101,558 1998-09-23
US13008699P 1999-04-20 1999-04-20
US69/130,086 1999-04-20
US15025599P 1999-08-23 1999-08-23
US60/150,255 1999-08-23

Publications (3)

Publication Number Publication Date
WO2000017365A2 WO2000017365A2 (en) 2000-03-30
WO2000017365A3 WO2000017365A3 (en) 2000-08-24
WO2000017365A8 true WO2000017365A8 (en) 2000-10-12

Family

ID=27379204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021989 WO2000017365A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Country Status (5)

Country Link
EP (1) EP1115870A2 (en)
CA (1) CA2339595A1 (en)
HU (1) HUP0104697A3 (en)
NZ (1) NZ510594A (en)
WO (1) WO2000017365A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
AU2001243939B2 (en) * 2000-03-28 2006-06-15 Queensland University Of Technology A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences
NZ521488A (en) 2000-03-28 2004-07-30 Univ Queensland A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
DE10049587A1 (en) 2000-10-06 2002-05-02 Icon Genetics Ag Vector system for plants
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
DE10061150A1 (en) 2000-12-08 2002-06-13 Icon Genetics Ag Methods and vectors for the production of transgenic plants
DE10102389A1 (en) 2001-01-19 2002-08-01 Icon Genetics Ag Methods and vectors for plastid transformation of higher plants
DE10109354A1 (en) * 2001-02-27 2002-09-05 Icon Genetics Ag Recombinant viral switch systems
DE10114209A1 (en) 2001-03-23 2002-12-05 Icon Genetics Ag Site-directed transformation using amplification vectors
DE10115507A1 (en) 2001-03-29 2002-10-10 Icon Genetics Ag Method for coding information in nucleic acids of a genetically modified organism
DE10121283B4 (en) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Methods and vectors for amplification or expression of desired nucleic acid sequences in plants
DE10132780A1 (en) 2001-07-06 2003-01-16 Icon Genetics Ag Plastid gene expression via autonomously replicating vectors
DE10143238A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Identification of eukaryotic internal ribosome entry sites (IRES) elements
DE10143237A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Manufacture of artificial internal ribosomal entry point elements (Ires elements)
US7238854B2 (en) 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
WO2004003180A1 (en) 2002-07-01 2004-01-08 E.I. Du Pont De Nemours And Company Method of controlling gene silencing using site-specific recombination
AU2007302306A1 (en) 2006-09-25 2008-04-03 Freie Universitat Berlin Transcriptional repressors of cytokinin signaling and their use
ES2385451T3 (en) 2009-07-10 2012-07-25 Freie Universität Berlin Rock2 and Rock3, two new variants of function gain of cytokinin receptors AHK2 and AHK3
EP2418283A1 (en) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
EP2584042A1 (en) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, storage and use of cell wall-degrading enzymes
EP2647715A1 (en) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
EP3097783B1 (en) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicin m for the control of ehec
EP3372091A1 (en) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Method of reducing contamination of an object with clostridium
EP3378485A1 (en) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bacteriocins for control of salmonella enterica
US11633467B2 (en) 2018-02-15 2023-04-25 Icon Genetics Gmbh Immunogenic composition and vaccine for generating an immune response to norovirus
ES2953232T3 (en) 2019-03-12 2023-11-08 Icon Genetics Gmbh Norovirus-like particles with improved stability
AU2020287918A1 (en) 2019-06-06 2021-12-16 Nomad Bioscience Gmbh Klebicins for the control of Klebsiella
WO2022012926A1 (en) 2020-07-16 2022-01-20 Nomad Bioscience Gmbh Products for oral consumption with reduced sugar content
EP4248987A1 (en) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Chimeric bacteriocins and method for the control of pseudomonas

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE80182T1 (en) * 1985-10-25 1992-09-15 Monsanto Co PLANT VECTORS.
NL9001711A (en) * 1989-10-03 1991-05-01 Clovis Matton N V GENETIC MANIPULATIONS WITH RECOMBINANT DNA INCLUDING SEQUENCES DERIVED FROM RNA VIRUS.
WO1994019477A1 (en) * 1993-02-26 1994-09-01 Calgene Inc. Geminivirus-based gene expression system
GB9405768D0 (en) * 1994-03-23 1994-05-11 Univ Leicester Viral replicon
GB9626858D0 (en) * 1996-12-24 1997-02-12 Gatsby Plant Science Innovatio Transcriptional regulation in plants
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
CA2306053C (en) * 1997-11-18 2003-01-21 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems

Also Published As

Publication number Publication date
CA2339595A1 (en) 2000-03-30
WO2000017365A3 (en) 2000-08-24
HUP0104697A3 (en) 2003-12-29
NZ510594A (en) 2002-09-27
EP1115870A2 (en) 2001-07-18
WO2000017365A2 (en) 2000-03-30
HUP0104697A2 (en) 2002-03-28

Similar Documents

Publication Publication Date Title
WO2000017365A8 (en) Binary viral expression system in plants
ES2180641T3 (en) CYTOPLASMIC INHIBITION OF GENETIC EXPRESSION.
WO2000007430A3 (en) Plant fatty acid desaturase promoters
WO1998044097A3 (en) Method for enhancing expression of a foreign or endogenous gene product in plants
HK1034999A1 (en) Dsrna-mediated regulation of gene expression in plants.
WO1995031564A3 (en) Method of introducing pathogen resistance in plants
WO2000053724A3 (en) Compositions and methods for the modification of gene transcription
UA40661C2 (en) vector for incorporation of desired gene into the plant (variants), a method for obtaining transgenic plant, a method for incorporation of at least two DESIRED genes into the plant
HUP0002578A2 (en) Male tissue-preferred regulatory region and method of using same
WO2000031283A3 (en) Regulatory sequences useful for gene expression in plant embryo tissue
FR2772787B1 (en) H3C4 PROMOTER OF BUT ASSOCIATED WITH THE FIRST INTRON OF RICE ACTINE, CHIMERIC GENE INCLUDING IT AND TRANSFORMED PLANT
DE60042178D1 (en) Genes encoding flavone synthases
WO2000026389A3 (en) Dna comprising rice anther-specific gene and transgenic plant transformed therewith
DE69731292D1 (en) PROMOTOR ELEMENTS THAT GIVE ROOT-PREFERRED GENE EXPRESSION
WO2000063397A3 (en) Methods for delivering inhibitory rna to plants and applications thereof
AU7532998A (en) Recombinant construct for enhancement of gene expression in plants
AU5559301A (en) Nucleic acids that control plant development
UA70915C2 (en) A method for inducing the viral resistance in plants
AU5539500A (en) Promoter enabling transgene expression in the whole plant except in the seed
WO1999055891A8 (en) Plant gene that regulates dna methylation
WO2001012799A3 (en) Regulatory sequences for pollen specific or pollen abundant gene expression in plants
MXPA01009366A (en) Plants having modified reproductive capacity.
WO1999051758A8 (en) Enzymes responsible for the metabolism of zeatin
WO2001005986A3 (en) Mites-like element and transcriptional activation element
HUP0202805A2 (en) Targeted gene removal

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref country code: AU

Ref document number: 2000 14410

Kind code of ref document: A

Format of ref document f/p: F

AK Designated states

Kind code of ref document: A2

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: C1

Designated state(s): AU BR CA HU IL JP KR MX NZ PL US

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 13/2000 UNDER (30) REPLACE "NOT FURNISHED" BY "60/150255"

ENP Entry into the national phase

Ref document number: 2339595

Country of ref document: CA

Ref country code: CA

Ref document number: 2339595

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1999969445

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 14410/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PA/a/2001/002374

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 09786676

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 510594

Country of ref document: NZ

WWP Wipo information: published in national office

Ref document number: 1999969445

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999969445

Country of ref document: EP