WO2000014273A3 - Technique de representation de differentiels genetiques et vecteur - Google Patents

Technique de representation de differentiels genetiques et vecteur Download PDF

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Publication number
WO2000014273A3
WO2000014273A3 PCT/CA1999/000789 CA9900789W WO0014273A3 WO 2000014273 A3 WO2000014273 A3 WO 2000014273A3 CA 9900789 W CA9900789 W CA 9900789W WO 0014273 A3 WO0014273 A3 WO 0014273A3
Authority
WO
WIPO (PCT)
Prior art keywords
length
mono
libraries
differential
vector
Prior art date
Application number
PCT/CA1999/000789
Other languages
English (en)
Other versions
WO2000014273A2 (fr
Inventor
Abdel Majid Belouchi
Helene Fournier
Mark Gee
Denis Gauvreau
Original Assignee
Signalgene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Signalgene Inc filed Critical Signalgene Inc
Priority to CA002342903A priority Critical patent/CA2342903A1/fr
Publication of WO2000014273A2 publication Critical patent/WO2000014273A2/fr
Publication of WO2000014273A3 publication Critical patent/WO2000014273A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé permettant d'obtenir une représentation différentielle de l'expression de gènes par comparaison de bibliothèques d'ARNc mono-longueurs. Ces bibliothèques sont hybridées par des sondes en réseaux ordonnés accessibles permettant de reconnaître les sites présentant des hybridations parmi les bibliothèques de segments mono-longueurs, et de localiser les gènes présentant un différentiel d'expression. L'invention porte également sur une bibliothèque de mono-longueurs correspondant à des, ou complémentaires de, séquences d'ARNm exprimé, comportant les éléments d'ARNc de 23 nucléotides de long et de formule 5'-gcuggagaucggnnnnnnnnnnn-3', dans laquelle n représente l'un des 11 ribonucléotides de la séquence correspondant à la séquence complémentaire de 11 nucléotides de l'ARNm.
PCT/CA1999/000789 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur WO2000014273A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002342903A CA2342903A1 (fr) 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14593698A 1998-09-03 1998-09-03
US09/145,936 1998-09-03

Publications (2)

Publication Number Publication Date
WO2000014273A2 WO2000014273A2 (fr) 2000-03-16
WO2000014273A3 true WO2000014273A3 (fr) 2000-06-02

Family

ID=22515207

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000789 WO2000014273A2 (fr) 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur

Country Status (2)

Country Link
CA (1) CA2342903A1 (fr)
WO (1) WO2000014273A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2808287B1 (fr) * 2000-04-26 2004-09-17 Ipsogen Methode de mesure quantitative de l'expression des genes
US20050014168A1 (en) * 2003-06-03 2005-01-20 Arcturus Bioscience, Inc. 3' biased microarrays

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013877A1 (fr) * 1995-10-12 1997-04-17 Lynx Therapeutics, Inc. Mesure de profils d'expression genique pour evaluer la toxicite
US5658736A (en) * 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation
WO1998015651A1 (fr) * 1996-10-04 1998-04-16 Brax Genomics Limited Identification de la liaison d'un oligonucleotide non codant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013877A1 (fr) * 1995-10-12 1997-04-17 Lynx Therapeutics, Inc. Mesure de profils d'expression genique pour evaluer la toxicite
US5658736A (en) * 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation
WO1998015651A1 (fr) * 1996-10-04 1998-04-16 Brax Genomics Limited Identification de la liaison d'un oligonucleotide non codant

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIANG P ET AL: "DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER RNA BY MEANS OF THE POLYMERASE CHAIN REACTION", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 257, no. 5072, 14 August 1992 (1992-08-14), pages 967 - 971, XP000508268, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2000014273A2 (fr) 2000-03-16
CA2342903A1 (fr) 2000-03-16

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