CA2342903A1 - Technique de representation de differentiels genetiques et vecteur - Google Patents

Technique de representation de differentiels genetiques et vecteur Download PDF

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Publication number
CA2342903A1
CA2342903A1 CA002342903A CA2342903A CA2342903A1 CA 2342903 A1 CA2342903 A1 CA 2342903A1 CA 002342903 A CA002342903 A CA 002342903A CA 2342903 A CA2342903 A CA 2342903A CA 2342903 A1 CA2342903 A1 CA 2342903A1
Authority
CA
Canada
Prior art keywords
length
mono
library
gene
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002342903A
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English (en)
Inventor
Abdel Majid Belouchi
Helene Fournier
Mark Gee
Denis Gauvreau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Signalgene Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2342903A1 publication Critical patent/CA2342903A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé permettant d'obtenir une représentation différentielle de l'expression de gènes par comparaison de bibliothèques d'ARNc mono-longueurs. Ces bibliothèques sont hybridées par des sondes en réseaux ordonnés accessibles permettant de reconnaître les sites présentant des hybridations parmi les bibliothèques de segments mono-longueurs, et de localiser les gènes présentant un différentiel d'expression. L'invention porte également sur une bibliothèque de mono-longueurs correspondant à des, ou complémentaires de, séquences d'ARNm exprimé, comportant les éléments d'ARNc de 23 nucléotides de long et de formule 5'-gcuggagaucggnnnnnnnnnnn-3', dans laquelle n représente l'un des 11 ribonucléotides de la séquence correspondant à la séquence complémentaire de 11 nucléotides de l'ARNm.
CA002342903A 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur Abandoned CA2342903A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14593698A 1998-09-03 1998-09-03
US09/145,936 1998-09-03
PCT/CA1999/000789 WO2000014273A2 (fr) 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur

Publications (1)

Publication Number Publication Date
CA2342903A1 true CA2342903A1 (fr) 2000-03-16

Family

ID=22515207

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002342903A Abandoned CA2342903A1 (fr) 1998-09-03 1999-08-26 Technique de representation de differentiels genetiques et vecteur

Country Status (2)

Country Link
CA (1) CA2342903A1 (fr)
WO (1) WO2000014273A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2808287B1 (fr) * 2000-04-26 2004-09-17 Ipsogen Methode de mesure quantitative de l'expression des genes
US20050014168A1 (en) * 2003-06-03 2005-01-20 Arcturus Bioscience, Inc. 3' biased microarrays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013877A1 (fr) * 1995-10-12 1997-04-17 Lynx Therapeutics, Inc. Mesure de profils d'expression genique pour evaluer la toxicite
US5658736A (en) * 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation
GB9620749D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Identifying antisense oligonucleotides

Also Published As

Publication number Publication date
WO2000014273A3 (fr) 2000-06-02
WO2000014273A2 (fr) 2000-03-16

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