WO2000003012A3 - Expression of eukaryotic peptides in plant plastids - Google Patents

Expression of eukaryotic peptides in plant plastids Download PDF

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Publication number
WO2000003012A3
WO2000003012A3 PCT/US1999/015473 US9915473W WO0003012A3 WO 2000003012 A3 WO2000003012 A3 WO 2000003012A3 US 9915473 W US9915473 W US 9915473W WO 0003012 A3 WO0003012 A3 WO 0003012A3
Authority
WO
WIPO (PCT)
Prior art keywords
plastid
eukaryotic
plant
constructs
peptides
Prior art date
Application number
PCT/US1999/015473
Other languages
French (fr)
Other versions
WO2000003012A9 (en
WO2000003012A2 (en
Inventor
Kevin E Mcbride
Narender Nehra
Douglas A Russell
David M Stalker
Jeffrey M Staub
Original Assignee
Calgene Llc
Kevin E Mcbride
Narender Nehra
Douglas A Russell
David M Stalker
Jeffrey M Staub
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/113,244 external-priority patent/US6512162B2/en
Application filed by Calgene Llc, Kevin E Mcbride, Narender Nehra, Douglas A Russell, David M Stalker, Jeffrey M Staub filed Critical Calgene Llc
Priority to EP99933798A priority Critical patent/EP1097211B1/en
Priority to BR9912019-4A priority patent/BR9912019A/en
Priority to CA002333148A priority patent/CA2333148A1/en
Priority to DK99933798T priority patent/DK1097211T3/en
Priority to JP2000559233A priority patent/JP2002520019A/en
Priority to DE69935857T priority patent/DE69935857T2/en
Publication of WO2000003012A2 publication Critical patent/WO2000003012A2/en
Publication of WO2000003012A3 publication Critical patent/WO2000003012A3/en
Publication of WO2000003012A9 publication Critical patent/WO2000003012A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
PCT/US1999/015473 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids WO2000003012A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP99933798A EP1097211B1 (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids
BR9912019-4A BR9912019A (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids
CA002333148A CA2333148A1 (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids
DK99933798T DK1097211T3 (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids
JP2000559233A JP2002520019A (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids
DE69935857T DE69935857T2 (en) 1998-07-10 1999-07-10 EXPRESSION OF EUCAROTIC PEPTIDES IN PLANT PLASTIDES

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/113,244 1998-07-10
US09/113,244 US6512162B2 (en) 1998-07-10 1998-07-10 Expression of eukaryotic peptides in plant plastids
US31684799A 1999-05-21 1999-05-21
US09/316,847 1999-05-21

Publications (3)

Publication Number Publication Date
WO2000003012A2 WO2000003012A2 (en) 2000-01-20
WO2000003012A3 true WO2000003012A3 (en) 2000-06-08
WO2000003012A9 WO2000003012A9 (en) 2000-07-20

Family

ID=26810845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/015473 WO2000003012A2 (en) 1998-07-10 1999-07-10 Expression of eukaryotic peptides in plant plastids

Country Status (11)

Country Link
US (2) US6812379B2 (en)
EP (1) EP1097211B1 (en)
JP (1) JP2002520019A (en)
AT (1) ATE360072T1 (en)
BR (1) BR9912019A (en)
CA (1) CA2333148A1 (en)
DE (1) DE69935857T2 (en)
DK (1) DK1097211T3 (en)
ES (1) ES2286886T3 (en)
PT (1) PT1097211E (en)
WO (1) WO2000003012A2 (en)

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US7741536B2 (en) 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
CN1330718A (en) * 1998-10-07 2002-01-09 辛根塔参与股份公司 Therapeutically active proteins in plants
US6815580B1 (en) 1999-05-13 2004-11-09 Monsanto Technology Llc Expression of the Chlorella sorokiniana sedoheptulose 1,7-bisphosphatase in transgenic plants
CN1360635A (en) * 1999-05-13 2002-07-24 孟山都技术有限公司 Expression of sedoheptulose 1,7-bisphosphatase in transgenic plants
EP1268832A4 (en) * 2000-02-29 2003-04-02 Univ Auburn Production of antibodies in transgenic plastids
US20030041353A1 (en) 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
CA2401958A1 (en) * 2000-02-29 2001-09-07 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
EP2080803B1 (en) * 2000-03-01 2011-05-25 Auburn University Plastid transformation vectors for expressing proinsulin fused to the cholera toxin B-subunit in plants
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GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
JP4838434B2 (en) * 2001-03-22 2011-12-14 財団法人地球環境産業技術研究機構 Protein expression system using plant body
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WO2005069986A2 (en) 2004-01-20 2005-08-04 Monsanto Technology Llc Chimeric promoters for use in plants
CA2613697A1 (en) * 2005-06-28 2007-01-04 Ventria Bioscience Components of cell culture media produced from plant cells
CN101263229A (en) * 2005-09-16 2008-09-10 拜尔农科股份有限公司 Transplastomic plants expressing lumen-targeted protein
CN101490266B (en) 2006-05-16 2012-06-27 孟山都技术有限公司 Use of non-agrobacterium bacterial species for plant transformation
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
EP2126096B1 (en) 2007-03-09 2013-12-18 Monsanto Technology, LLC Methods for plant transformation using spectinomycin selection
WO2008118394A1 (en) 2007-03-23 2008-10-02 New York University Methods of affecting nitrogen assimilation in plants
EP2463370B1 (en) 2007-06-01 2013-07-31 Sapphire Energy, Inc. Use of genetically modified organisms to generate biomass degrading enzymes
GB2463543A (en) * 2007-09-11 2010-03-24 Sapphire Energy Inc Molecule production by photosynthetic organisms
EP2203542A1 (en) * 2007-09-11 2010-07-07 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
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Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009829A2 (en) * 1988-04-07 1989-10-19 Massachusetts Institute Of Technology Methods of generating desired amino-terminal residues in proteins
WO1991002800A1 (en) * 1989-08-14 1991-03-07 Advanced Technologies (Cambridge) Ltd. Thylakoid targeting sequence
EP0437320A1 (en) * 1990-01-10 1991-07-17 Korea Institute Of Science And Technology Recombinant DNA and its use in producing human insulin
WO1991013993A1 (en) * 1990-03-05 1991-09-19 The Upjohn Company Protein expression via seed specific regulatory sequences
WO1995016783A1 (en) * 1993-12-14 1995-06-22 Calgene Inc. Controlled expression of transgenic constructs in plant plastids
WO1995024492A1 (en) * 1994-03-11 1995-09-14 Calgene Inc. Expression of bacillus thuringiensis cry proteins in plant plastids
WO1997017453A1 (en) * 1995-11-06 1997-05-15 Prodigene Commercial production of aprotinin in plants
WO1997042313A1 (en) * 1996-05-03 1997-11-13 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1998006860A1 (en) * 1996-08-14 1998-02-19 Novartis Ag Peptide with inhibitory activity towards plant pathogenic fungi
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors
WO1998011235A2 (en) * 1996-09-12 1998-03-19 Novartis Ag Transgenic plants expressing cellulolytic enzymes
CA2188220A1 (en) * 1996-10-18 1998-04-18 Kimberley Kenward Non-food crop plant bioreactor
WO1998026083A1 (en) * 1996-12-13 1998-06-18 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US5773705A (en) * 1992-12-31 1998-06-30 Wisconsin Alumni Research Foundation Ubiquitin fusion protein system for protein production in plants
WO1998031823A1 (en) * 1997-01-17 1998-07-23 The Scripps Research Institute Rna binding protein and binding site useful for expression of recombinant molecules
WO1998058069A1 (en) * 1997-06-17 1998-12-23 Monsanto Company Expression of fructose 1,6 bisphosphate aldolase in transgenic plants
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2612874B2 (en) * 1986-10-02 1997-05-21 マサチユセツツ・インスチチユート・オブ・テクノロジー Methods of regulating protein metabolic stability
JP2753274B2 (en) * 1988-08-24 1998-05-18 株式会社三和化学研究所 Method for producing motilin-like polypeptide, recombinant DNA therefor and plasmid for expression
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein

Patent Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009829A2 (en) * 1988-04-07 1989-10-19 Massachusetts Institute Of Technology Methods of generating desired amino-terminal residues in proteins
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1991002800A1 (en) * 1989-08-14 1991-03-07 Advanced Technologies (Cambridge) Ltd. Thylakoid targeting sequence
EP0437320A1 (en) * 1990-01-10 1991-07-17 Korea Institute Of Science And Technology Recombinant DNA and its use in producing human insulin
WO1991013993A1 (en) * 1990-03-05 1991-09-19 The Upjohn Company Protein expression via seed specific regulatory sequences
US5773705A (en) * 1992-12-31 1998-06-30 Wisconsin Alumni Research Foundation Ubiquitin fusion protein system for protein production in plants
WO1995016783A1 (en) * 1993-12-14 1995-06-22 Calgene Inc. Controlled expression of transgenic constructs in plant plastids
WO1995024492A1 (en) * 1994-03-11 1995-09-14 Calgene Inc. Expression of bacillus thuringiensis cry proteins in plant plastids
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors
WO1997017453A1 (en) * 1995-11-06 1997-05-15 Prodigene Commercial production of aprotinin in plants
WO1997042313A1 (en) * 1996-05-03 1997-11-13 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
WO1998006860A1 (en) * 1996-08-14 1998-02-19 Novartis Ag Peptide with inhibitory activity towards plant pathogenic fungi
WO1998011235A2 (en) * 1996-09-12 1998-03-19 Novartis Ag Transgenic plants expressing cellulolytic enzymes
CA2188220A1 (en) * 1996-10-18 1998-04-18 Kimberley Kenward Non-food crop plant bioreactor
WO1998026083A1 (en) * 1996-12-13 1998-06-18 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
WO1998031823A1 (en) * 1997-01-17 1998-07-23 The Scripps Research Institute Rna binding protein and binding site useful for expression of recombinant molecules
WO1998058069A1 (en) * 1997-06-17 1998-12-23 Monsanto Company Expression of fructose 1,6 bisphosphate aldolase in transgenic plants
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BAKER R.: "Protein expression using ubiquitin fusion and cleavage", CURRENT OPINION IN BIOTECHNOLOGY, vol. 7, 1996, pages 541 - 546, XP002132162 *
DANIELL ET AL: "Containment of herbicide resistance through genetic engineering of the chloroplast genome", NATURE BIOTECHNOLOGY, vol. 16, 1 April 1998 (1998-04-01), pages 345 - 348, XP002090409, ISSN: 1087-0156 *
KWON S Y ET AL: "EXPRESSION OF ACTIVE HUMAN INTERLEUKIN -6 IN TRANSGENIC TOBACCO", MOLECULES AND CELLS, (31 OCT 1995) VOL. 5, NO. 5, PP. 486-492. ISSN: 1016-8478., XP000884550 *
LEE J S ET AL: "Establishment of a transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor.", MOLECULES AND CELLS, (1997 DEC 31) 7 (6) 783-7., XP000884527 *
MAREK, LAURA FREDRICK ET AL: "Chlamydomonas chloroplast transformation using human carbonic anhydrase.", PLANT PHYSIOLOGY (ROCKVILLE), (1994) VOL. 105, NO. 1 SUPPL., PP. 84. MEETING INFO.: ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS PORTLAND, OREGON, USA JULY 30-AUGUST 3, 1994, XP002118154 *
MATSUMOTO S ET AL: "CHARACTERIZATION OF A HUMAN GLYCOPROTEIN ( ERYTHROPOIETIN ) PRODUCED IN CULTURED TOBACCO CELLS", PLANT MOLECULAR BIOLOGY, (MAR 1995) VOL. 27, NO. 6, PP. 1163-1172. ISSN: 0167-4412., XP002132161 *
MCBRIDE ET AL: "Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticial protein in tobacco", BIO/TECHNOLOGY, vol. 13, 13 April 1995 (1995-04-13), pages 362 - 365, XP002090404, ISSN: 0733-222X *
SAFFORD R. ET AL.: "Regulated expression of the rat medium chain hydrolase gene in transgenic rape seed", TRANSGENIC RESEARCH, vol. 2, 1993, pages 191 - 198, XP000884365 *

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Publication number Publication date
WO2000003012A9 (en) 2000-07-20
EP1097211B1 (en) 2007-04-18
JP2002520019A (en) 2002-07-09
US6812379B2 (en) 2004-11-02
BR9912019A (en) 2002-02-19
DE69935857T2 (en) 2008-01-10
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CA2333148A1 (en) 2000-01-20
DK1097211T3 (en) 2007-06-04
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US20050283848A1 (en) 2005-12-22
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