EP1268832A4 - Production of antibodies in transgenic plastids - Google Patents

Production of antibodies in transgenic plastids

Info

Publication number
EP1268832A4
EP1268832A4 EP01918262A EP01918262A EP1268832A4 EP 1268832 A4 EP1268832 A4 EP 1268832A4 EP 01918262 A EP01918262 A EP 01918262A EP 01918262 A EP01918262 A EP 01918262A EP 1268832 A4 EP1268832 A4 EP 1268832A4
Authority
EP
European Patent Office
Prior art keywords
antibodies
production
transgenic plastids
plastids
transgenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01918262A
Other languages
German (de)
French (fr)
Other versions
EP1268832A1 (en
Inventor
Henry Daniell
Keith Wycoff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Auburn University
University of Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auburn University, University of Central Florida filed Critical Auburn University
Publication of EP1268832A1 publication Critical patent/EP1268832A1/en
Publication of EP1268832A4 publication Critical patent/EP1268832A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1275—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
EP01918262A 2000-02-29 2001-02-28 Production of antibodies in transgenic plastids Withdrawn EP1268832A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18566100P 2000-02-29 2000-02-29
US185661P 2000-02-29
PCT/US2001/006274 WO2001064929A1 (en) 2000-02-29 2001-02-28 Production of antibodies in transgenic plastids

Publications (2)

Publication Number Publication Date
EP1268832A1 EP1268832A1 (en) 2003-01-02
EP1268832A4 true EP1268832A4 (en) 2003-04-02

Family

ID=22681926

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01918262A Withdrawn EP1268832A4 (en) 2000-02-29 2001-02-28 Production of antibodies in transgenic plastids

Country Status (4)

Country Link
EP (1) EP1268832A4 (en)
AU (1) AU2001245358A1 (en)
CA (1) CA2401965A1 (en)
WO (1) WO2001064929A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44266E1 (en) 1997-01-17 2013-06-04 The Scripps Research Institute Expression of eukaryotic polypeptides in chloroplasts
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
EP2080803B1 (en) * 2000-03-01 2011-05-25 Auburn University Plastid transformation vectors for expressing proinsulin fused to the cholera toxin B-subunit in plants
WO2001083529A2 (en) 2000-04-28 2001-11-08 Planet Biotechnology, Inc. Immunoadhesin for the prevention of rhinovirus infection
US20020168367A1 (en) * 2000-04-28 2002-11-14 Planet Biotechnology Incorporated Novel immunoadhesins for treating and preventing viral and bacterial diseases
CN1886512B (en) * 2002-04-23 2015-11-25 斯克里普斯研究所 The expression of polypeptide in chloroplast(id) and for the composition of express polypeptide and method
AU2008202053B2 (en) * 2002-04-23 2012-11-01 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
US20070124838A1 (en) 2002-07-03 2007-05-31 Henry Daniell Expression of the human igf-1 in transgenic plastids
SI1523496T1 (en) 2002-07-18 2011-11-30 Merus B V Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
DK1737971T3 (en) 2004-01-20 2017-11-13 Merus Nv MIXTURES OF BINDING PROTEINS
WO2006066340A1 (en) * 2004-12-21 2006-06-29 Grain Biotech Australia Pty Ltd A method for the high expression of immunoglobulin in plants
EP1885173A4 (en) 2005-05-27 2009-02-18 Univ Central Florida Chloroplasts engineered to express pharmaceutical proteins
AU2008232543B2 (en) 2007-03-30 2013-01-17 University Of Central Florida Research Foundation, Inc. Chloroplasts engineered to express pharmaceutical proteins in edible plants
US8268553B2 (en) 2007-06-01 2012-09-18 Sapphire Energy, Inc. High throughput screening of genetically modified photosynthetic organisms
GB2463337B (en) 2007-09-11 2012-11-21 Sapphire Energy Inc Methods of producing organic products with photosynthetic organisms
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
WO2011057243A2 (en) 2009-11-09 2011-05-12 University Of Central Florida Research Foundation, Inc. Administration of plant expressed oral tolerance agents
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
WO2011097603A1 (en) 2010-02-08 2011-08-11 Regeneron Pharmaceuticals, Inc. Common light chain mouse
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
US20140056897A1 (en) 2011-03-10 2014-02-27 Hco Antibody, Inc. Bispecific three-chain antibody-like molecules
WO2012122512A1 (en) 2011-03-10 2012-09-13 Hco Antibody, Inc. Recombinant production of mixtures of single chain antibodies
IL273982B2 (en) 2011-08-05 2023-03-01 Regeneron Pharma Humanized universal light chain mice
US10865419B2 (en) 2011-10-24 2020-12-15 The Trustees Of The University Of Pennsylvania Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes
EA035344B1 (en) 2012-04-20 2020-05-29 Мерюс Н.В. Method for producing two antibodies from a single host cell
RU2016141307A (en) 2014-03-21 2018-04-24 Регенерон Фармасьютикалз, Инк. EXCELLENT HUMAN ANIMALS THAT MAKE SINGLE-DOMAIN BINDING PROTEINS
AU2016232715A1 (en) 2015-03-19 2017-09-28 Regeneron Pharmaceuticals, Inc. Non-human animals that select for light chain variable regions that bind antigen
WO2021178388A1 (en) * 2020-03-02 2021-09-10 Ventria Bioscience Inc. Enhanced expression yield of immunoglobulin a in eukaryotes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5925806A (en) * 1995-06-06 1999-07-20 Mcbride; Kevin E. Controlled expression of transgenic constructs in plant plastids
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
USRE44266E1 (en) * 1997-01-17 2013-06-04 The Scripps Research Institute Expression of eukaryotic polypeptides in chloroplasts
AR020090A1 (en) * 1998-06-15 2002-04-10 John Innes Ct MONOCOTILEDONE VEGETABLE CELL OR SEED OF HER, PLANT OR PART OF A PLANT, METHOD FOR MAKING A MONOCOTILEDONE VEGETABLE CELL, METHOD FOR MAKING AN APPLIANCE, USE OF AN EXPRESSION CONSTRUCTION TO PRODUCE A PLANT CELL AND SED OF CONSTRUCTION EXPRESSION
WO2000003012A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of eukaryotic peptides in plant plastids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5925806A (en) * 1995-06-06 1999-07-20 Mcbride; Kevin E. Controlled expression of transgenic constructs in plant plastids
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO2000020612A2 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of WO0164929A1 *
SVAB ET AL: "high-frequency plastid transformation in tobacco by selection for a chimeric aadA gene", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 90, February 1993 (1993-02-01), pages 913 - 917, XP002106110, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
CA2401965A1 (en) 2001-09-07
WO2001064929A1 (en) 2001-09-07
EP1268832A1 (en) 2003-01-02
AU2001245358A1 (en) 2001-09-12

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