WO1999066325A1 - Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides - Google Patents

Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides Download PDF

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Publication number
WO1999066325A1
WO1999066325A1 PCT/SE1999/000828 SE9900828W WO9966325A1 WO 1999066325 A1 WO1999066325 A1 WO 1999066325A1 SE 9900828 W SE9900828 W SE 9900828W WO 9966325 A1 WO9966325 A1 WO 9966325A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
protein
solution
reagent
spa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/SE1999/000828
Other languages
English (en)
French (fr)
Inventor
Franz Steindl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech AB filed Critical Amersham Pharmacia Biotech AB
Priority to AT99928306T priority Critical patent/ATE452337T1/de
Priority to JP2000555093A priority patent/JP4399115B2/ja
Priority to EP99928306A priority patent/EP1088227B1/en
Priority to US09/719,512 priority patent/US6706486B1/en
Priority to DE69941815T priority patent/DE69941815D1/de
Publication of WO1999066325A1 publication Critical patent/WO1999066325A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Definitions

  • thermostable immunoligands The above mentioned IgG binding proteins, polypeptides and peptides (binding outside the paratope) are relatively resistant to heat treatment (in solution) and will therefore in the present document be referred to as thermostable immunoligands. 30 These proteins (particularly protein A) are used in a variety of applications and are employed on a large scale mainly in the purification of monoclonal and polyclonal antibodies. Both the native and recombinasnt forms are used as ligands in immunoaffinity chromatography . This refined 35 technology provides highly effective purification of antibodies from complex solutions.
  • a buffer and/or protein are added before the heating step in order to compensate for sample variations in pH and in protein content, respectively.
  • a chelator may be added before the heating step in order to bind heavy metal ions that may be present .
  • the chelating compound may be any chelator that efficiently chelates the various heavy metal ions that may be present in the samples contemplated or as contaminants in any of the added reagents. Examples are EDTA (ethylene diamine tetraacetic acid) and DETAPAC (diethylene triamine pentaacetic acid) .
  • the added chelator should be present in the sample before the heating step within the concentration range of 0.5 to 5 mM.
  • the dilution ratio can be reduced, maintaining an appropriate protein: SDS ratio.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
PCT/SE1999/000828 1998-06-19 1999-05-14 Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides Ceased WO1999066325A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT99928306T ATE452337T1 (de) 1998-06-19 1999-05-14 Verfahren für die quantitative freisetzung von natürlichen oder rekombinanten proteinen, polypeptiden oder peptiden
JP2000555093A JP4399115B2 (ja) 1998-06-19 1999-05-14 天然または組換えタンパク質、ポリペプチドまたはペプチドの定量的解離の方法
EP99928306A EP1088227B1 (en) 1998-06-19 1999-05-14 Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides
US09/719,512 US6706486B1 (en) 1998-06-19 1999-05-14 Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides
DE69941815T DE69941815D1 (de) 1998-06-19 1999-05-14 Verfahren für die quantitative freisetzung von natürlichen oder rekombinanten proteinen, polypeptiden oder peptiden

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT106898 1998-06-19
ATA1068/98 1998-06-19

Publications (1)

Publication Number Publication Date
WO1999066325A1 true WO1999066325A1 (en) 1999-12-23

Family

ID=3505919

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1999/000828 Ceased WO1999066325A1 (en) 1998-06-19 1999-05-14 Method for the quantitative release of natural or recombinant proteins, polypeptides or peptides

Country Status (7)

Country Link
US (1) US6706486B1 (https=)
EP (1) EP1088227B1 (https=)
JP (1) JP4399115B2 (https=)
AT (1) ATE452337T1 (https=)
DE (1) DE69941815D1 (https=)
ES (1) ES2337639T3 (https=)
WO (1) WO1999066325A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003058229A1 (fr) * 2001-12-28 2003-07-17 Japan Science And Technology Agency Procede d'electrophorese de proteines

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008078809A1 (ja) * 2006-12-27 2008-07-03 Japan Science And Technology Agency 鳥類抗体を使用する免疫学的検出方法
WO2011093745A1 (en) * 2010-01-26 2011-08-04 Obschestvo S Ogranichennoi Otvetstvennostyu "Nauchno- Proizvodstvennaya Firma "Materia Medika Kholding" Method of determination of autoantibody level by means of enzyme immunoassay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0213093A1 (en) * 1985-06-03 1987-03-04 Pharmacia Ab Immunoassay method for the determination of Fc-part binding bacterial polypeptides and/or corresponding antibodies
US5556745A (en) * 1994-06-03 1996-09-17 Sch+E,Uml U+Ee Pbach; J+E,Uml O+Ee Rg Method for the detection and quantitative determination of antigen in a test sample containing immune complexes of antigen bound to antibodies and to rheumatoid factors
AT403378B (de) * 1995-09-11 1998-01-26 Steindl Franz Dipl Ing Dr Immun- und proteinkomplex-dissoziierung

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4092114A (en) 1976-10-20 1978-05-30 Fisher Scientific Company Indirect latex test for determination of immunoglobulins
JP2791367B2 (ja) 1988-04-21 1998-08-27 マイクロプローブ・コーポレーション 核酸抽出方法
KR100230909B1 (ko) 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0213093A1 (en) * 1985-06-03 1987-03-04 Pharmacia Ab Immunoassay method for the determination of Fc-part binding bacterial polypeptides and/or corresponding antibodies
US5556745A (en) * 1994-06-03 1996-09-17 Sch+E,Uml U+Ee Pbach; J+E,Uml O+Ee Rg Method for the detection and quantitative determination of antigen in a test sample containing immune complexes of antigen bound to antibodies and to rheumatoid factors
AT403378B (de) * 1995-09-11 1998-01-26 Steindl Franz Dipl Ing Dr Immun- und proteinkomplex-dissoziierung

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003058229A1 (fr) * 2001-12-28 2003-07-17 Japan Science And Technology Agency Procede d'electrophorese de proteines
US7708870B2 (en) 2001-12-28 2010-05-04 Japan Science And Tecnology Agency Method of electrophoresing protein

Also Published As

Publication number Publication date
ES2337639T3 (es) 2010-04-27
JP2002518675A (ja) 2002-06-25
EP1088227B1 (en) 2009-12-16
EP1088227A1 (en) 2001-04-04
ATE452337T1 (de) 2010-01-15
JP4399115B2 (ja) 2010-01-13
US6706486B1 (en) 2004-03-16
DE69941815D1 (de) 2010-01-28

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