WO1999061894A1 - Dispositif pour la detection de substances en phase fluide - Google Patents

Dispositif pour la detection de substances en phase fluide Download PDF

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Publication number
WO1999061894A1
WO1999061894A1 PCT/EP1999/003513 EP9903513W WO9961894A1 WO 1999061894 A1 WO1999061894 A1 WO 1999061894A1 EP 9903513 W EP9903513 W EP 9903513W WO 9961894 A1 WO9961894 A1 WO 9961894A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
light guide
spectra
detector
detector cell
Prior art date
Application number
PCT/EP1999/003513
Other languages
German (de)
English (en)
Inventor
Ralf God
Lutz MÜLLER-KUHRT
Original Assignee
Analyticon Ag Biotechnologie Pharmazie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Analyticon Ag Biotechnologie Pharmazie filed Critical Analyticon Ag Biotechnologie Pharmazie
Priority to AU42657/99A priority Critical patent/AU4265799A/en
Publication of WO1999061894A1 publication Critical patent/WO1999061894A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors

Definitions

  • the invention relates to a device for the detection of substances in a fluid phase according to the preamble of claim 1.
  • DE 195 45 423 AI describes a multi-channel detector that works according to the light absorption principle of a UV / VIS detector.
  • the separate components are passed through individual detector cells.
  • the cells have windows made of transparent quartz glass, through which the sample flowing through the supply line is exposed to UV / VIS light. beam is irradiated.
  • the multi-channel detector described here it is not clear how the light is supplied to each detector cell and how the light passed through the cells is further processed in terms of signals by each detector cell, so that the result of each substance that was to be determined in the detector cells is an evaluable signal is obtained.
  • EP 0529541 AI describes a two-beam detector for high pressure liquid chromatography.
  • a light beam from a light source is imaged on two detector cells. Since a spectral decomposition of the light is carried out here before it enters the detector cell, this principle cannot be applied to several channels.
  • the object of the invention is to provide a device with which a parallel registration of a plurality of UV absorption or fluorescence emission spectra for the multi-channel detection of substances in liquid chromatographic separation processes or other analysis processes is possible.
  • the light from a UV and / or VIS light source is coupled into an optical waveguide.
  • the beam from the light source is divided into several beam paths, which corresponds to the number of detector cells present.
  • the attenuated light emerging from the detector cells is sent to a spectral ter fed.
  • the light beams supplied to the spectrophotometer via the light guides are spectrally broken down and placed on the sensor field of a flat light sensor, e.g. B. a CCD camera.
  • a CCD camera it is also possible to use any other area-like light sensor that allows separate reading out of several structures.
  • a darkening device is additionally installed in the device, which interrupts the light beam path to the light sensor briefly and if necessary rhythmically.
  • the location for this darkening device can be anywhere, but it has proven advantageous to attach it either between the light source and the light coupler or after a light guide and in front of the light sensor.
  • a motor-controlled iris diaphragm or a dark surface or mirror surface introduced into the beam path by means of a motor, for example, can be used as the darkening device.
  • 2a shows an arrangement for generating light and coupling the light into a light guide
  • 3a shows an arrangement for beam division into 24 channels and a reference channel
  • 5a shows an arrangement of the light guides for light -
  • 5b shows a detailed arrangement of 24 light guide channels in the form of a linear arrangement
  • FIG. 6 shows an image of the spectra on the CCD sensor field of the CCD camera
  • 7a shows a device according to the invention in combination with a liquid chromatography arrangement
  • Fig.l shows a schematic representation of the detection device according to the invention.
  • the light coming from a light source 1 is fed to a light guide bundle 4 in a light coupler 40.
  • This can e.g. B. can be achieved by means of a mirror or lens system.
  • An optical fiber controller 41 enables the mechanical separation of the individual optical fiber channels 42.
  • the divided optical fiber channels 42 are connected to a detector cell block 20 on a light input side of the sample transmission area 2.
  • the detector cell block 20 contains detector cells 21.
  • the arrangement of the detector cells 21 can also be carried out separately, ie not combined in a detector cell block 20.
  • Each individual light guide channel 42 is light-guiding with a detector cell 21, z. B. coupled via a standardized SMA connector.
  • the here with the detector cells 21, z. B. mechanically combined via a standardized SMA connector, coupled light guide channels 52 in a light guide assembly 50 to form a light guide bundle 5.
  • Each optical fiber channel 42 and 52 transmits its spectral information independently of the others.
  • the light guide bundle 5 is connected in a light guide feed 51 to a spectrophotometer 30 in a light-guiding manner. After the spectral decomposition of the individual channels 52 guided light rays in the spectrophotometer 30 of the light emerging from the light guide bundle 5 takes place in a flat light sensor 31, here designed as a CCD camera, the registration of the spectra.
  • a control unit 32 and a computer 33 make it possible for the spectra recorded in parallel but locally separated by the light guide arrangement to be read out and processed separately. In this way, simultaneous UV or fluorescence detection of substances in liquid chromatographic processes is advantageously possible with only one light source and one sensor unit.
  • the light source 1 is explained in more detail in FIGS. 2a and 2b.
  • the light for the UV and / or VIS range is generated with the aid of a deuterium lamp 10 and / or a tungsten lamp 11.
  • the arrangement is such that the light 12 emitted by the tungsten lamp 11 shines through the hollow cathode of a deuterium lamp 10 and then the entire lamp spectrum onto a light guide bundle 4, for. B. is mapped by means of mirrors or lenses.
  • an optical fiber Y coupling can alternatively be used for superimposing the light 12 of the tungsten lamp 11 and the light 14 of the deuterium lamp 10.
  • the light enters the light guide bundle 4.
  • the light can already be superimposed at the Y coupling or can be guided in separate light guides 15 and 16 to the detector cells 21 and only superimposed on the detector cells 21.
  • Such a lamp combination is not required if only the UV or only the VIS range is required. If only a certain extinction wavelength is required in fluorescence spectrometry, this is generated, for example, by a corresponding grid arrangement between the light source 1 and the light guide bundle 4.
  • the light source for example, by a corresponding grid arrangement between the light source 1 and the light guide bundle 4.
  • a light guide splitter 41 is described by way of example in FIGS. 3a and 3b.
  • the light guide bundle 4 exposed to light is mechanically divided into twenty-five light guide channels 42 via the light guide splitter 41, twenty-four light guide channels 42 leading light to the detector cells 21 and one light guide channel as a light guide reference channel 44 being fed directly to a spectrophotometer 30.
  • the detector cell block 20 shows a detector cell block 20 with individual detector cells 21.
  • the detector cell block 20 consists of a black quartz glass body 23.
  • the quartz glass body 23 has bores 24 which are designed as detector cells 21.
  • the bores 24 are provided on the light input side and light output side with a window 25 made of transparent quartz glass, so that the detector cells 21 thus created can be irradiated with light.
  • the eluent stream of a liquid chromatographic system can be fed to the detector cells 21, on the one hand, and can be derived again after flowing through the detector cells 21.
  • Incident light 28 passes through the detector cells 21 and is continued as a weakened emerging light 29.
  • the 4b shows detector cells 21 for measuring fluorescence spectra.
  • the detector cells 21 contain a further window 25 for the detection of fluorescence light 22.
  • This window 25 is arranged parallel to the light path so that the fluorescent light 22 can be detected at an angle of approximately 90 ° to the light path.
  • the light guide channels 52 are mechanically combined in a light guide bundle 50 in a light guide bundle 5 such that each individual light guide channel 52 independently transmits spectral information.
  • the light guide bundle 5 is then connected to the spectrophotometer 30 via the light guide feed 51.
  • the light guide channels 52 are separated here again and arranged linearly in the form of a slit arrangement 54 such that, after spectral decomposition, the respective absorption or fluorescence spectra can be imaged in parallel onto the sensor field 34 of the CCD camera 31 .
  • Spacers 55 are placed in the gap arrangement 54 between the linearly arranged light guide channels 52.
  • FIG. 6 shows the sensor field 34 of the CCD camera 31, on which several UV or fluorescence spectra are imaged in parallel.
  • the control unit 32 for the CCD camera 31 and the computer 33 ensure that the spectra can be registered and read out and processed further within short time intervals.
  • the device according to the invention is used as follows in a method for the multiparallel registration of the UV or fluorescence spectra of substances in liquid chromatography.
  • the eluent currents 60 become one of the detector cell block 20 fed.
  • the chromatographic system here consists of a pump 61, an injector 62 for sample application and a chromatography column 63.
  • An outlet 64 of the chromatic system is provided on the light output side on the detector cells 21 of the detector cell block 20. If a substance dissolved in the eluent stream flows through a detector cell 21, this absorbs a certain part of the light passing through the detector cell 21 or emits characteristic fluorescent light 22 over time in accordance with its electronic properties.
  • the light 29 emerging from the detector cells 21 then delivers spectral decomposition in the spectrophotometer 30 over time either the characteristic UV absorption spectra or the fluorescence spectra of the substance which flows through the detector cell 21.
  • This process takes place in parallel in all detector cells 21, so that the UV absorption spectra 38 and the UV absorption spectra 39 or the fluorescence emission spectra are registered in a temporally resolved manner by all detector cells 21.
  • This method is shown schematically for the UV adsorption measurement in FIG.
  • Light guide 3 spectra registration device light guide

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne un dispositif pour la détection de substances en phase fluide au moyen de spectres UV ou de spectres de fluorescence, comportant une source de lumière (1), une zone d'irradiation de l'échantillon (2) et un système d'enregistrement de spectres (3). L'invention a pour objet un dispositif permettant un enregistrement parallèle de plusieurs spectres d'absorption d'UV ou d'émission de fluorescence en vue de la détection multicanal de substances dans des procédés de séparation par chromatographie en phase liquide ou d'autres procédés d'analyse. Dans le dispositif décrit, la lumière d'une source de lumière UV et/ou visible est injectée dans une optique à guide de lumière. Dans cette dernière, le faisceau de la source de lumière est divisé en plusieurs trajets correspondant au nombre des cellules détectrices existantes.
PCT/EP1999/003513 1998-05-25 1999-05-22 Dispositif pour la detection de substances en phase fluide WO1999061894A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU42657/99A AU4265799A (en) 1998-05-25 1999-05-22 Device for detecting substances in fluid phase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998124652 DE19824652A1 (de) 1998-05-25 1998-05-25 Vorrichtung zur Detektion von flüssigchromatographisch getrennten Substanzen mittels UV- oder Fluoreszenzspektren
DE19824652.8 1998-05-25

Publications (1)

Publication Number Publication Date
WO1999061894A1 true WO1999061894A1 (fr) 1999-12-02

Family

ID=7869683

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/003513 WO1999061894A1 (fr) 1998-05-25 1999-05-22 Dispositif pour la detection de substances en phase fluide

Country Status (3)

Country Link
AU (1) AU4265799A (fr)
DE (1) DE19824652A1 (fr)
WO (1) WO1999061894A1 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012051529A1 (fr) * 2010-10-15 2012-04-19 Lockheed Martin Corporation Conception optique microfluidique
US9067207B2 (en) 2009-06-04 2015-06-30 University Of Virginia Patent Foundation Optical approach for microfluidic DNA electrophoresis detection
CN104865331A (zh) * 2015-03-09 2015-08-26 俞嘉德 高效液相色谱兼用紫外可见和荧光双分光双检测仪
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
CN106018403A (zh) * 2016-05-12 2016-10-12 刘马禾 阵列毛细管电泳仪的光吸收检测器及检测方法
CN107643261A (zh) * 2017-09-25 2018-01-30 珠海广睿汇利发展有限公司 一种长光程怀特池doas法测量污染气体浓度的监测仪
WO2020190969A1 (fr) * 2019-03-18 2020-09-24 Life Technologies Corporation Système de détection optique multi-capillaire
CN112304915A (zh) * 2020-10-29 2021-02-02 苏州雅睿生物技术有限公司 一种实时荧光检测光学系统、实时荧光定量pcr仪
WO2021071572A3 (fr) * 2019-08-07 2021-06-10 Life Technologies Corporation Système de détection optique multi-capillaire
WO2022122577A3 (fr) * 2020-12-09 2022-07-21 Ams-Osram Ag Spectromètre d'absorbance optique, dispositif optique et procédé de spectrométrie d'absorbance optique

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Publication number Priority date Publication date Assignee Title
DE29901464U1 (de) * 1999-01-28 2000-07-06 J & M Analytische Mess & Regeltechnik Gmbh Kombinationslichtquelle und Analysesystem unter Verwendung derselben
DE10054426B4 (de) * 2000-10-27 2006-03-09 Iom Innovative Optische Messtechnik Gmbh Verfahren zur Multi-Fluoreszenz-Detektion
DE10222822A1 (de) * 2002-05-21 2003-12-04 Conducta Endress & Hauser Online-Analysator
US9182336B2 (en) 2012-03-02 2015-11-10 Laxco, Inc. Multichannel analytical instruments for use with specimen holders
RU207285U1 (ru) * 2021-03-03 2021-10-21 Общество с ограниченной ответственностью "Радиоэлектронные механико-оптические системы" Устройство для регистрации искр, возгораний и радиационных загрязнений

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EP0109536A1 (fr) * 1982-10-15 1984-05-30 Kabushiki Kaisha Toshiba Dispositif pour l'analyse d'absorption
DE3247355A1 (de) * 1982-12-22 1984-06-28 Merck Patent Gmbh, 6100 Darmstadt Geraet zur quantitativen auswertung von duennschichtchromatogrammen
JPS6073343A (ja) * 1983-09-30 1985-04-25 Shimadzu Corp 分光光度計
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EP0616211B1 (fr) * 1993-03-18 1999-01-13 Novartis AG Dispositif de détection optique pour l'analyse chimique de petits volumes d'échantillons fluides

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Publication number Priority date Publication date Assignee Title
EP0109536A1 (fr) * 1982-10-15 1984-05-30 Kabushiki Kaisha Toshiba Dispositif pour l'analyse d'absorption
DE3247355A1 (de) * 1982-12-22 1984-06-28 Merck Patent Gmbh, 6100 Darmstadt Geraet zur quantitativen auswertung von duennschichtchromatogrammen
JPS6073343A (ja) * 1983-09-30 1985-04-25 Shimadzu Corp 分光光度計
US5210590A (en) * 1992-02-18 1993-05-11 L. T. Industries, Inc. Rapid scanning spectrographic analyzer
DE19528855A1 (de) * 1995-08-05 1997-02-06 Leybold Ag Verfahren und Vorrichtung zur spektralen Remissions- und Transmissionsmessung

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Cited By (20)

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US9649631B2 (en) 2009-06-04 2017-05-16 Leidos Innovations Technology, Inc. Multiple-sample microfluidic chip for DNA analysis
US9067207B2 (en) 2009-06-04 2015-06-30 University Of Virginia Patent Foundation Optical approach for microfluidic DNA electrophoresis detection
US9656261B2 (en) 2009-06-04 2017-05-23 Leidos Innovations Technology, Inc. DNA analyzer
GB2497501A (en) * 2010-10-15 2013-06-12 Lockheed Corp Micro fluidic optic design
US8961764B2 (en) 2010-10-15 2015-02-24 Lockheed Martin Corporation Micro fluidic optic design
WO2012051529A1 (fr) * 2010-10-15 2012-04-19 Lockheed Martin Corporation Conception optique microfluidique
US9988676B2 (en) 2012-02-22 2018-06-05 Leidos Innovations Technology, Inc. Microfluidic cartridge
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
CN104865331A (zh) * 2015-03-09 2015-08-26 俞嘉德 高效液相色谱兼用紫外可见和荧光双分光双检测仪
CN106018403A (zh) * 2016-05-12 2016-10-12 刘马禾 阵列毛细管电泳仪的光吸收检测器及检测方法
CN106018403B (zh) * 2016-05-12 2019-05-21 南京擎科生物科技有限公司 阵列毛细管电泳仪的光吸收检测器及检测方法
CN107643261A (zh) * 2017-09-25 2018-01-30 珠海广睿汇利发展有限公司 一种长光程怀特池doas法测量污染气体浓度的监测仪
US20220155225A1 (en) * 2019-03-18 2022-05-19 Life Technologies Corporation Multi-capillary optical detection system
WO2020190969A1 (fr) * 2019-03-18 2020-09-24 Life Technologies Corporation Système de détection optique multi-capillaire
US11982620B2 (en) 2019-03-18 2024-05-14 Life Technologies Corporation Multi-capillary optical detection system
WO2021071572A3 (fr) * 2019-08-07 2021-06-10 Life Technologies Corporation Système de détection optique multi-capillaire
CN112304915A (zh) * 2020-10-29 2021-02-02 苏州雅睿生物技术有限公司 一种实时荧光检测光学系统、实时荧光定量pcr仪
CN112304915B (zh) * 2020-10-29 2021-05-04 苏州雅睿生物技术有限公司 一种实时荧光检测光学系统、实时荧光定量pcr仪
WO2022122577A3 (fr) * 2020-12-09 2022-07-21 Ams-Osram Ag Spectromètre d'absorbance optique, dispositif optique et procédé de spectrométrie d'absorbance optique
TWI815235B (zh) * 2020-12-09 2023-09-11 奧地利商Ａｍｓ有限公司 光學吸光度光譜儀、光學裝置及光學吸光度光譜測定之方法

Also Published As

Publication number Publication date
AU4265799A (en) 1999-12-13
DE19824652A1 (de) 2000-01-20

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