WO1999055851A3 - Transformation de plantes dirigee sur un site - Google Patents

Transformation de plantes dirigee sur un site Download PDF

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Publication number
WO1999055851A3
WO1999055851A3 PCT/EP1999/002813 EP9902813W WO9955851A3 WO 1999055851 A3 WO1999055851 A3 WO 1999055851A3 EP 9902813 W EP9902813 W EP 9902813W WO 9955851 A3 WO9955851 A3 WO 9955851A3
Authority
WO
WIPO (PCT)
Prior art keywords
site
plants
recognition sites
present
directed transformation
Prior art date
Application number
PCT/EP1999/002813
Other languages
English (en)
Other versions
WO1999055851A2 (fr
Inventor
Annmarie Bloom Tuttle
Erica Judith Pascal
Janet Louise Suttie
Mary-Dell Chilton
Original Assignee
Novartis Ag
Novartis Erfind Verwalt Gmbh
Annmarie Bloom Tuttle
Erica Judith Pascal
Janet Louise Suttie
Chilton Mary Dell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Novartis Erfind Verwalt Gmbh, Annmarie Bloom Tuttle, Erica Judith Pascal, Janet Louise Suttie, Chilton Mary Dell filed Critical Novartis Ag
Priority to AU39288/99A priority Critical patent/AU3928899A/en
Publication of WO1999055851A2 publication Critical patent/WO1999055851A2/fr
Publication of WO1999055851A3 publication Critical patent/WO1999055851A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention décrit des procédés pour l'intégration dirigée sur un site d'un transgène dans un génome de plante, à l'aide de systèmes de recombinase et de recombinaison dirigés sur un site. L'invention décrit l'utilisation de deux sites de reconnaissance de recombinase différents, en particulier, deux sites de reconnaissance de recombinase qui différent dans leurs segments espaceurs. L'invention concerne également des plantes transgéniques obtenues par le procédé selon l'invention, et leur descendance.
PCT/EP1999/002813 1998-04-28 1999-04-26 Transformation de plantes dirigee sur un site WO1999055851A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39288/99A AU3928899A (en) 1998-04-28 1999-04-26 Site-directed transformation of plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6755298A 1998-04-28 1998-04-28
US09/067,552 1998-04-28

Publications (2)

Publication Number Publication Date
WO1999055851A2 WO1999055851A2 (fr) 1999-11-04
WO1999055851A3 true WO1999055851A3 (fr) 2000-01-20

Family

ID=22076767

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/002813 WO1999055851A2 (fr) 1998-04-28 1999-04-26 Transformation de plantes dirigee sur un site

Country Status (2)

Country Link
AU (1) AU3928899A (fr)
WO (1) WO1999055851A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0937098B1 (fr) 1995-06-07 2002-08-14 Invitrogen Corporation Clonage de recombinaison in vitro au moyen de sites recombinaison obtenus par genie genetique
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
CA2307016A1 (fr) 1997-10-24 1999-05-06 Life Technologies, Inc. Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison
ES2256972T3 (es) 1997-11-18 2006-07-16 Pioneer Hi-Bred International, Inc. Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas.
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
ATE454459T1 (de) 1997-11-18 2010-01-15 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
PT1034262E (pt) 1997-11-18 2005-10-31 Pioneer Hi Bred Int Composicoes e metodos para a modificacao genetica de plantas
WO2001011058A1 (fr) * 1999-08-09 2001-02-15 Monsanto Technology Llc Nouveaux procedes et vecteurs de clonage
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
DE10303937A1 (de) * 2003-01-31 2004-08-12 Icon Genetics Ag Pflanzentransformation mit Assemblierung einer gewünschten Sequenz in Vivo
AU2004207177B2 (en) 2003-01-31 2008-02-07 Bayer Cropscience N.V. Plant transformation with in vivo assembly of a trait
WO2005059148A1 (fr) * 2003-12-17 2005-06-30 Pioneer Hi-Bred International, Inc. Systemes de piegeage de genes a mediation par recombinase
US20070015195A1 (en) 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Modified FRT recombination site libraries and methods of use
WO2007137114A2 (fr) 2006-05-17 2007-11-29 Pioneer Hi-Bred International, Inc. Minichromosomes de plantes artificielles
CA2691440A1 (fr) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Procedes de modification du genome d'une cellule de plante monocotyledone
WO2009114321A2 (fr) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Méganucléases rationnellement conçues pour modification par génie génétique du génome du maïs

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025821A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Compositions et procedes de modification genetique de plantes
WO1999025840A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Nouveau procede d'integration d'adn etranger dans des genomes .

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999025821A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Compositions et procedes de modification genetique de plantes
WO1999025840A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Nouveau procede d'integration d'adn etranger dans des genomes .
WO1999025854A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Procede de transformation stable et dirigee de cellules eucaryotes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BETHKE B, SAUER B: "SEGMENTAL GENOMIC REPLACEMENT BY Cre-MEDIATED RECOMBINATION: GENOTOXIC STRESS ACTIVATION OF THE p53PROMOTER IN SINGLE-COPY TRANFORMANTS", NUCLEIC ACIDS RESEARCH, vol. 25, no. 14, 1997, pages 2828 - 2834, XP002122405 *
LYZNIK ET AL: "FLP-mediated recombination of FRT sites in the maize genome", NUCLEIC ACIDS RESEARCH, vol. 24, no. 19, 1 January 1996 (1996-01-01), pages 3784 - 3789, XP002096357, ISSN: 0305-1048 *
LYZNIK L A ET AL: "Activity of yeast FLP recombinase in maize and rice protoplasts", NUCLEIC ACIDS RESEARCH, vol. 21, no. 4, 25 February 1993 (1993-02-25), pages 969 - 975, XP002089984, ISSN: 0305-1048 *
SCHLAKE T ET AL: "USE OF MUTATED FLP RECOGNITION TARGET (FRT) SITES FOR THE EXCHANGE OF EXPRESSION CASSETTES AT DEFINED CHROMOSOMAL LOCI", BIOCHEMISTRY, vol. 33, no. 43, 1 November 1994 (1994-11-01), pages 12746 - 12751, XP000616165, ISSN: 0006-2960 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US9309520B2 (en) 2000-08-21 2016-04-12 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Also Published As

Publication number Publication date
AU3928899A (en) 1999-11-16
WO1999055851A2 (fr) 1999-11-04

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