WO1999055152A1 - Conservation de materiel vegetal - Google Patents

Conservation de materiel vegetal Download PDF

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Publication number
WO1999055152A1
WO1999055152A1 PCT/GB1999/001242 GB9901242W WO9955152A1 WO 1999055152 A1 WO1999055152 A1 WO 1999055152A1 GB 9901242 W GB9901242 W GB 9901242W WO 9955152 A1 WO9955152 A1 WO 9955152A1
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Prior art keywords
petals
volume
medium
vol
preservation medium
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PCT/GB1999/001242
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English (en)
Inventor
Frank Gordon Riddell
Jennifer Martha Mcdonald Elliott
Margaret Louise Carstairs
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The University Court Of The University Of St. Andrews
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Priority to AU36183/99A priority Critical patent/AU3618399A/en
Publication of WO1999055152A1 publication Critical patent/WO1999055152A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax

Definitions

  • the present invention relates to a method of preserving plant material, and in particular to a method of preserving flowers.
  • O-A-95/85828 describes a method of preserving plant material.
  • the method employed therein consists of immersing the plant material in an aqueous solution containing between 40% and 95% by weight of one or more C 3 -C 6 dihydric alcohols at a temperature of between 60 and 95°C.
  • the addition of transition metal salts to the preservation medium, in particular copper salts, assists in the retention of chlorophyll in the stems and leaves.
  • the preserved material is indicated to have a life of over three months.
  • the pH of the aqueous solution is kept in the range from 3 to 9, preferably about pH 7.
  • O-A-95/85828 also describes the use of a dip-coating step where the plant is immersed in an aqueous solution or suspension of one or more polymers followed by drying to provide a polymer coating on the plant.
  • O-A-95/85828 appears to be effective in preserving the colour and texture of a variety of plant materials. However, it has been found that it is not entirely effective in preserving the colour and texture of plant materials coloured with red, orange and/or yellow pigments and/or dyes, and in particular, but not exclusively, plant materials containing anthocyanins .
  • the present invention provides a method for the preservation of plant material, said method comprising exposing said plant material to a preservation medium comprising 65-98% of one or more alcohols, and having a pH below 3.
  • the preservation medium comprises one or more dihydric or polyhydric alcohols.
  • the preservation medium may further comprise one or more monohydric alcohols, for example propan-2-ol.
  • the alcohols are preferably selected from ethylene glycol , propylene glycol , 1, 2-propanediol , 1 , 4-butanediol , 1, 5-pentanediol , 1 , 6-hexanediol , glycerol, polyethylene glycols, propan-2-ol, and mixtures thereof.
  • the preservation media of the present invention may include mixtures of any of the following alcohols in the quantities stated: % by volume 1,2-propanediol 25-90% 1,4-butanediol 0-75% 1, 5-pentanediol 0-50% 1,6-hexanediol 0-50% propan-2-ol 0-10% water 0-35% polyethyleneglycol 0-25% glycerol 0-10%
  • the alcohols present in the preservation media are a mixture of 1, 2-propanediol , 1 , 6-hexanediol and 1, 4-butanediol .
  • Suitable quantities include 25-90% by volume 1 , 2-propanediol ; 0-75% by volume 1,4- butanediol; and 0-50% by volume 1, 6-hexanediol .
  • the preservation medium comprises 50-90% by volume 1, 2-propandiol , 10-40% by volume 1 , 4-butanediol and 0-25% by volume 1, 6-hexanediol .
  • the pH of the preservation medium is lowered by the addition of an acid to the medium.
  • the acid is non-volatile.
  • the method is directed towards the preservation of flowers.
  • the method provides for the preservation of red, orange, yellow, purple, pink, mauve and peach flowers.
  • the method provides for the preservation of plant material containing pH sensitive pigments and/or dyes.
  • the plant material preserved will be non-chlorophyll -containing, i.e will be the non-green portions of a plant such as bracts or flowers. Petals are of special interest.
  • the method preferably comprises the following steps:
  • the preservation medium may comprise 25-75 vol% 1, 2-propanediol and 25-75 vol% 1 , 4-butanediol and 5-25 vol% 1, 6-hexanediol, water, 1, 5-pentanediol , propan-2- ol, PEG 400, PEG 600 and/or glycerol, and 0.5 to 15 g/litre acid preservation medium.
  • the acid may be any suitable acid capable of reducing the pH of the preservation medium to below 3, preferably to a pH of 2 or lower.
  • strong acids are preferred.
  • Mention may be made of benzenesulphonic acid, citric acid, hydrochloric acid, trifluoroacetic acid, toluenesulphonic acids, naphthalene- 1-sulphonic acid and naphthalene-2 -sulphonic acid.
  • Other acids also that work include acetic acid (especially glacial acetic acid added in amounts of 100 ml or more per litre medium) , formic acid (especially 97% formic acid, added in amounts of 100 ml or more per litre medium) , phosphoric acid and sulphuric acid.
  • the preservation media may be acidified by the addition of metal salts as described further below.
  • the acid is hydrochloric acid (20-50 ml/litre medium) , para-toluenesulphonic acid (20 g/litre medium) or citric acid (100-200 g/litre medium) .
  • the pH of the preservation medium is 2 or less.
  • the preservation medium may contain up to 35 vol% water.
  • the plant material is treated with the preservation medium for 5-45 minutes, most preferably 20-40 minutes, however a treatment time of around 30 minutes is most preferred.
  • the preservation medium temperature is 60- 90°C, for example around 80°C.
  • the water and/or propan-2-ol used to rinse the excess preservation medium from the plant material is preferably of ambient temperature. Desirably the treated plant material is washed with water, with propan-2-ol, or with water followed by propan-2-ol.
  • Light stabilisation of the petals (or other plant matter) containing anthocyanin pigments when preserved with an acid medium can be enhanced by including one or more salts comprising any of the following metal ions in the preservation medium:
  • Red pigments Cr 3+ , Cu 2+ , Ca 2+ , Fe 2+ , Fe 3+ , Sn 2+ , Mg 2+ , Zn 2+ , Ni 2+ , Al 3+ , Ba 2+ Yellow pigments: Sn 2+ , Cr 3+ , Cu 2+ , Al 3+ , Zn 2+ , Fe 2+ , Fe 3+
  • the metal ions may be added in the form of any salt which dissolves or is adequately dispersed in the medium. Combinations of metal salts providing one or more metal ions may be used. Desirably, the metal salts are present at a concentration of 5 to 30 g/litre in the preservation medium, preferably 8 to 15 g/litre, for example at 10 g/litre preservation medium.
  • Red pigments are preferably light stabilised using salts containing Cr 3+ , Cu 2+ , Ca 2+ , Fe 2+ , Fe 3+ or Sn 2+ .
  • Yellow pigments are preferably light stabilised using salts containing Al 3+ , Cr 3+ , Cu + , Fe 2+ , Fe 3+ or Sn 2+ .
  • the salts may themselves acidify the medium to the pH required and hence separate addition of acid is not required.
  • Examples of such salts and the pH obtained when the salt is added at a concentration of 10 g/litre preservation medium is set out below: p ⁇ Stannous chloride 1.05 Ferric chloride 0.80 Ferrous sulphate 2.29 Ammonium ferric sulphate 1.72
  • the plant material may be treated with a preservation medium as defined hereinabove which has not been acidified.
  • the thus preserved plant material may then be acidified to a pH of less than 3 thus restoring the colour of the preserved plant material.
  • the best survival post treatment was from petals treated with mixtures of 1, 2-propanediol (25-75 vol%) with 1,4-butanediol (5-75 vol%) and 1 , 6-hexanediol (0- 25 vol%) , with 20 g/litre to 50 g/litre para- toluenesulphonic acid and CuS0 4 .5H 2 0 at 10 g/litre.
  • Petals treated in this way showed excellent colour preservation and petal texture after several weeks whilst during that period similar petal or rose heads not so treated shrivelled, dehydrated and lost their lustrous red colours.
  • Petals treated with the medium described in Example 1 (which contains water) showed signs of drying out.
  • our preferred method for the preservation of red rose heads is to use a mixture of 1,2-propanediol (25-75 vol%) , 1, 4-butanediol (5-75 vol%) and 1, 6-hexanediol (0-25 vol%) , 20 g/litre of para-toluene sulphonic acid and CuS0 4 .5H 2 0 (lOg/litre) and to treat the rose heads for approximately 30 minutes at around 80 °C.
  • Dipping the treated plant material into an aqueous emulsion of a vinylidene chloride acrylate polymer gives the petals a flexible coating, reduces solvent less, moisture absorption and reduces the severity of formation of crystals of 1 , 6-hexanediol on the surface of the plant material .
  • Other polymer coatings which exhibit flexibility and are at least partially impermeable to air, water and/or solvents could be used instead.
  • Comparative Example 1 A preservation medium comprising 1 , 2-propanediol (60 vol% by volume), 1 , 4-butanediol (10 vol%) , propan-2-ol (5 vol%) and water (25 vol%) containing cupric sulphate pentahydrate (lOg per litre of total solution) was prepared. Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief washing in cold water caused the red colour to disappear although the texture of the petals was preserved. The petals were colourless after the treatment. The pH of this medium is above 3.
  • a preservation medium comprising 1, 2-propanediol (60 vol%) , 1,4-butanediol (10 vol%) , propan-2-ol (5 vol%) and water (25 vol%) was prepared.
  • the petals were colourless after the treatment.
  • the pH of this medium is around 4-5.
  • a preservation medium comprising 1, 2-propanediol (60 vol%) and 1, 4-butanediol (40 vol%) with no water was prepared.
  • a preservation medium comprising 1, 2-propanediol (50 vol%) and 1 , 4-butanediol (50 vol%) with no water was prepared.
  • a preservation medium comprising 1 , 2-propanediol (25 vol%) and 1, 4-butanediol (75 vol%) with no water was prepared.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared.
  • Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in off white coloured petals.
  • Preservation media comprising 1, 2-propanediol (60 vol%) , 1,4-butanediol (10 vol%) , propan-2-ol (5 vol%) and water (25 vol%) were produced and varying amounts of concentrated hydrochloric acid (125 ml/litre; 50 ml/litre; 5 ml/litre) were added to the media. 10 Treatment of red rose petals with these media at 80°C for periods between 5 and 30 minutes followed by brief washing in cold water resulted in preservation of the red colour and the texture of the petals, except in long exposures at 125 ml/litre hydrochloric acid when the texture of the petal tissue was destroyed. The pH of these media are 2 or lower.
  • Preservation media comprising 1, 2-propanediol (60 vol%) and 1, 4-butanediol (40 vol%) were prepared. Varying amounts of concentrated hydrochloric acid (50 ml/litre; 25 ml/litre; 5 ml/litre) was then added. Treatment of red rose petals with these media at 80°C for periods between 5 and 30 minutes followed by brief washing in cold water resulted in preservation of the red colour in the petals and the texture of the petals. The pH of these media are 2 or lower.
  • Preservation media comprising 1 , 2-propanediol (50 vol%) and 1, 4-butanediol (50 vol%) were prepared. Varying 11 amounts of concentrated hydrochloric acid (50 ml/litre; 25 ml/litre; 5 ml/litre) . Treatment of red rose petals with these media at 80°C for periods between 5 and 30 minutes followed by brief washing in cold water resulted in preservation of the red colour in the petals and the texture of the petals. The pH of these media are 1 or lower.
  • Preservation media comprising 1 , 2-propanediol (25 vol%) and 1 , 4-butanediol (75 vol%) were prepared. Varying amounts of concentrated hydrochloric acid (50 ml/litre; 25 ml/litre; 5 ml/litre) were then added. Treatment of red rose petals with these media at 80 °C for periods between 5 and 30 minutes followed by brief washing in cold water resulted in preservation of the red colour in the petals and the texture of the petals. The pH of these media are 1 or lower.
  • a preservation media comprising 1, 2-propanediol (25 vol%) and 1 , 4-butanediol (75 vol%) were prepared. Concentrated hydrochloric acid was then added up to 20 ml per litre of preservation medium. Treatment of a red rose head with this medium at 80 °C for a period of 25 minutes followed by brief washing in cold water resulted in preservation of the red colour in all the petals and preserved the texture of the flower head. The pH of this medium is 2 or lower. 12
  • a pre-preservation medium comprising 1, 2-propanediol (60 vol%) , 1,4-butanediol (10 vol%) , propan-2-ol (5 vol%) and water (25 vol%) was prepared.
  • a preservation media comprising 1, 2-propanediol (60 vol%) and 1, 4-butanediol (40 vol%) were prepared. Concentrated hydrochloric acid was then added up to 2.5 g per litre of preservation medium. Treatment of red tulip petals with this medium at 80 °C for 5 minutes followed by brief washing in cold water preserved the colour of the petals as well as their texture and resilience. The pH of this medium is 2 or lower.
  • a preservation medium comprising 1, 4-butanediol (75% by volume) and 1 , 2-propanediol (25% by volume) was prepared. To this was added trifluoroacetic acid (20g per litre of total solution) was prepared. Treatment of red rose petals with this medium at 80 'C for periods 13 of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in crimson coloured petals.
  • the example was repeated five times but replacing the trifluoroacetic acid with one of benzensulphonic acid, sulphuric, phosphoric, naphthalene- 1-sulphuric and naphthalene-2 -sulphuric acid (each at 20 g/litre) . Good results were obtained with each acid.
  • a preservation medium comprising 1 , 4-butanediol (75% by volume) and 1, 2 -propanediol (25% by volume), was prepared. To this was added glacial acetic acid (100ml per litre) . Treatment of red rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep pink coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (50% by volume) and 1, 2 -propanediol (50% by volume) was prepared. To this was added 97% formic acid (125 ml per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in bright pink coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (50% by volume) and 1 , 2 -propanediol (50% by volume) was prepared. To this was added p-toluenesulphonic acid (25g per litre) . Treatment of red rose petals with 14 this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep red coloured petals close to the colour of the original petals.
  • a preservation medium comprising 1, 4-butanediol (5% by volume), 1 , 2-propanediol (70% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added citric acid (lOOg per litre) . Treatment of crimson rose petals with this medium at 80 ⁇ C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale crimson coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (5% by volume), 1, 2 -propanediol (70% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added citric acid (200g per litre) . Treatment of crimson rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep crimson coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume) and 1 , 2 -propanediol (90% by volume) was prepared. To this was added naphthalene- 1-sulphonic acid (20g per litre) and cupric sulphate pentahydrate (10g per litre) . Treatment of red rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient 15 temperature resulted in deep red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume) and 1, 2 -propanediol (90% by volume) was prepared. To this was added naphthalene-2-sulphonic acid (20g per litre) and cupric sulphate pentahydrate (10g per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume) and 1 , 2 -propanediol (90% by volume) was prepared. To this was added concentrated sulphuric acid (20 ml per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume) and 1, 2 -propanediol (90% by volume) was prepared. To this was added concentrated phosphoric acid (20 ml per litre) and cupric sulphate pentahydrate (10g per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep red coloured petals.
  • Example 18 16 A preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and aluminium potassium sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and aluminium sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and barium sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at 17 ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and calcium sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and chromium (III) sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in red coloured petals with a purple edge.
  • a preservation medium comprising 1 , 4-butanediol (10% by 18 volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ammonium ferric sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals with purple streaks. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals with purple streaks.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ferrous sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured 19 petals with purple streaks. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in deep purple coloured petals with pink streaks.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ferric chloride (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep purple coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in deep purple coloured petals.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and magnesium sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals.
  • a preservation medium comprising 1, 4-butanediol (10% by 20 volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and nickel sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pink coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and stannous chloride (lOg per litre) . Treatment of red rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in deep purple coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in deep purple coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and zinc sulphate (lOg per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with 21 continuous exposure to 20,000 LUX for 7 days resulted in pale red coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) . Treatment of red rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in deep pink coloured petals. Thus the treated plant material exhibited some fading following exposure to light. The light fastness of the material may be improved by inclusion of metal salts in the preservation medium (see for example, Examples 29 and 30) .
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and aluminium potassium sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale yellow coloured petals. 22 Example 33
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and aluminium sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale yellow coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and barium sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals. Addition of the barium salt in media to treat yellow petals did not give a lightfast product, although preservation was good.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- 23 hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and calcium sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80°C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals. Addition of the calcium salt in media to treat yellow petals did not yield a lightfast product, although preservation was good.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and chromium (III) sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in lemon yellow coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 'C for periods 24 of between 5 and 30 minutes followed by brief dipping in water at room temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale yellow coloured petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ammonium ferric sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale purple coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in pale yellow coloured petals with blue black streaks.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ferrous sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in lilac coloured petals with blue streaks. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in yellowy green coloured petals with blue streaks. 25 Example 40
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and ferric chloride (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in blue black coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in blue black coloured petals.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and magnesium sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals. Thus the addition of a magnesium salt in media to treat yellow petals did not produce a lightfast preserved product.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was 26 added p-toluenesulphonic acid (20g per litre) and nickel sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale pink coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals. Thus the addition of a nickel salt in media to treat yellow petals did not produce a lightfast preserved product.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and stannous chloride (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in golden yellow coloured petals. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in bright yellow coloured petals.
  • a preservation medium comprising 1, 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and zinc sulphate (lOg per litre) . Treatment of pale yellow rose petals with this medium at 80 "C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow 27 coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in off white petals.
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) . Treatment of pale yellow rose petals with this medium at 80 * C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pale yellow coloured petals with pink edges. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals. Thus the preserved product did not exhibit the additional advantage of lightfastness .
  • a preservation medium comprising 1 , 4-butanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,6- hexanediol (25% by volume) was prepared.
  • Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in white petals.
  • the preserved product did not exhibit the additional advantage of lightfastness .
  • Example 47 28 A preservation medium comprising 1 , 5-pentanediol (50% by volume) and 1, 2 -propanediol (50% by volume) was prepared. To this was added p-toluenesulphonic acid (25g per litre) . Treatment of bright pink rose petals with this medium at 80 'C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in pink coloured petals close to the colour of the original petals. The petals had a slightly oily appearance.
  • a preservation medium comprising 1 , 6-hexanediol (50% by volume) and 1, 2 -propanediol (50% by volume) was prepared. To this was added p-toluenesulphonic acid (25g per litre) . Treatment of red rose petals with this medium at 80 °C for periods of between 5 and 30 minutes followed by brief dipping in water at ambient temperature resulted in red coloured petals close to the colour of the original petals. The petals were found to be firmer than those obtained from media containing only diols of lower molecular weight than hexanediol. The surface appearance of the petals was good. Over a period of approximately four weeks crystals of 1, 6 -hexanediol appeared on the surface of the petals.
  • a preservation medium comprising 1, 6-hexanediol (25% by volume), 1, 2 -propanediol (65% by volume) and 1,4- butanediol (10% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate (lOg per litre) . Treatment of red rose heads with this medium at 80 'C for periods of between 30 and 60 minutes followed by brief dipping in water or 29 isopropanol at ambient temperature resulted in red coloured rose heads close to the colour of the originals. The heads were found to be firmer than those obtained from media containing only diols of lower molecular weight than hexanediol . The surface appearance of the petals was good. Over a period of approximately four weeks crystals of 1, 6-hexanediol appeared on the surface of the petals.
  • a preservation medium comprising 1, 6-hexanediol (10% by volume), 1, 2 -propanediol (65% by volume) and 1,4- butanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 "C for periods of between 30 and 60 minutes followed by brief dipping in water or isopropanol at ambient temperature resulted in red coloured rose heads close to the colour of the originals. The heads were found to be firm. The surface appearance of the petals was good. Over a period of three months no crystals of 1, 6-hexanediol appeared on the surface of the petals.
  • a preservation medium comprising 1, 6-hexanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,4- butanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 °C for periods of between 30 and 60 minutes followed by brief dipping in water or isopropanol at ambient temperature resulted in red coloured rose heads close to the colour of the originals. Subsequent treatment 30 of the heads in a light box with continuous exposure to 20,000 LUX for 5 days resulted in no loss of colour.
  • a preservation medium comprising 1, 6-hexanediol (25% by volume), 1 , 2-propanediol (65% by volume) and 1,4- butanediol (10% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 * C for periods of between 30 and 60 minutes followed by brief dipping in water or isopropanol at ambient temperature resulted in red coloured rose heads close to the colour of the originals. The rose heads were then dipped in a solution of polyvinylidene chloride.
  • Polidene 33-004 a vinylidene chloride-acrylate copolymer emulsion, supplied by Scott Bader Company Limited. Polidene 33-004 is a 50% solid-containing aqueous emulsion. It was used as a dilute emulsion after being diluted 10 times with water. The resulting rose heads had a similar colour and texture to the original heads. The polymer coating greatly reduced emergence of hexanediol on the surface of the heads.
  • a preservation medium comprising 1, 6-hexanediol (10% by volume), 1 , 2-propanediol (65% by volume) and 1,4- butanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 "C for periods of between 30 and 60 minutes followed by brief dipping in water or isopropanol at ambient temperature resulted in red coloured rose heads close 31 to the colour of the originals. The rose heads were then dipped in a solution of polyvinylidene chloride.
  • Polidene 33-004 a vinylidene chloride-acrylate copolymer emulsion, supplied by Scott Bader Company Limited. Polidene 33-004 is a 50% solid-containing aqueous emulsion. It was used as a dilute emulsion after being diluted 10 times with water. The resulting rose heads had a similar colour and texture to the original heads. No crystals of hexanediol had appeared on the surface of the heads after 3 months.
  • a preservation medium comprising 1 , 6-hexanediol (25% by volume), 1 , 2-propanediol (65% by volume), 1,4- butanediol (5% by volume) and glycerol (5% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (10g per litre) . Treatment of red rose heads with this medium at 80°C for periods of between 30 and 60 minutes followed by brief dipping in isopropanol at ambient temperature resulted in red coloured heads close to the colour of the originals. Emergence of crystals of hexanediol was greatly reduced compared to a control in which the there was no glycerol and there was 10% by volume 1 , 4 -butanediol .
  • a preservation medium comprising 1 , 6-hexanediol (20% by volume), 1, 2 -propanediol (65% by volume), 1,4- butanediol (10% by volume) and polyethylene glycol 400 (PEG 400) (5% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 "C 32 for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in red coloured heads close to the colour of the originals.
  • a preservation medium comprising 1 , 2 -propanediol (65% by volume), 1 , 4-butanediol (10% by volume) and polyethylene glycol 400 (PEG 400) (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red rose heads with this medium at 80 * C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in red coloured heads close to the colour of the originals. The heads had a slightly oily appearance.
  • a preservation medium comprising 1, 2 -propanediol (65% by volume), 1 , 4-butanediol (25% by volume) and 1,6- hexanediol (10% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red carnation heads with this medium at 80 "C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in red coloured heads close to the colour of the originals.
  • Example 58 33 A preservation medium comprising 1 , 2-propanediol (65% by volume), 1, 4 -butanediol (25% by volume) and 1,6- hexanediol (10% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of pink carnation heads with this medium at 80 °C for a period of 20 to 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in pink coloured heads with an orange tinge.
  • a preservation medium comprising 1, 2 -propanediol (65% by volume), 1 , 4-butanediol (25% by volume) and 1,6- hexanediol (10% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of purple irises with white and yellow centres to the flowers with this medium at 80 ⁇ C for a period of 25 minutes followed by brief dipping in isopropanol at ambient temperature resulted in coloured heads with a lighter purple colour and retaining the characteristic white and yellow markings.
  • a preservation medium comprising 1, 2 -propanediol (65% by volume), 1 , 4-butanediol (10% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of red tulip heads with this medium at 80 'C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in red coloured heads close to the colour of the originals. 34
  • p-toluenesulphonic acid 20g per litre
  • cupric sulphate pentahydrate lOg per litre
  • a preservation medium comprising 1 , 2 -propanediol (65% by volume), 1, 4 -butanediol (10% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added p-toluenesulphonic acid (20g per litre) and cupric sulphate pentahydrate (lOg per litre) . Treatment of yellow tulip heads with this medium at 80 °C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in yellow coloured heads close to the colour of the originals .
  • a preservation medium comprising 1 , 2 -propanediol (65% by volume), 1 , 4-butanediol (10% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added 10 g/litre ferric chloride to yield a medium having a pH of approximately 0.8. Treatment of red rose petals with this medium at 80°C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature resulted in purple petals which were light stable. (No fading after treatment in a light box with continuous exposure to 20,000 LUX for 7 days . )
  • a preservation medium comprising 1, 2 -propanediol (65% by volume), 1 , 4-butanediol (10% by volume) and 1,6- hexanediol (25% by volume) was prepared. To this was added 10 g/litre stannous chloride to give a pH of approximately 1.05. Treatment of red rose petals with this medium at 80°C for a period of 30 minutes followed by brief dipping in isopropanol at ambient temperature 35 resulted in pale crimson petals with purple streaks. Subsequent treatment of the petals in a light box with continuous exposure to 20,000 LUX for 7 days resulted in deep pink petals with purple streaks.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé permettant de traiter un matériel végétal afin de le conserver dans sa forme colorée. Le procédé comprend les étapes suivantes: a) on traite le matériel végétal avec un milieu de conservation ayant un pH inférieur à 3, pendant 1 à 90 minutes entre 60 et 90 °C; et b) on le lave avec de l'eau et/ou du propane-2-ol afin d'éliminer le milieu de conservation en excès. Une composition préférée du milieu de conservation comprend 50 à 90 % en volume de 1,2-propanediol, 10 à 40 % en volume de 1,4-butanediol et 0 à 25 % en volume de 1,6-hexanediol. On peut obtenir le pH bas nécessaire par addition d'acide ; on préfère pour cela l'acide p-toluène-sulfonique. Ce procédé convient pour traiter les pétales de fleur. On peut éventuellement ajouter des sels métalliques (tels que le CuSO4) pour améliorer la stabilisation à la lumière du matériel végétal, par exemple les pétales de rose rouges ou jaunes.
PCT/GB1999/001242 1998-04-24 1999-04-23 Conservation de materiel vegetal WO1999055152A1 (fr)

Priority Applications (1)

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AU36183/99A AU3618399A (en) 1998-04-24 1999-04-23 Preservation of plant material

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GB9808637.4 1998-04-24
GBGB9808637.4A GB9808637D0 (en) 1998-04-24 1998-04-24 Preservative

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1016343A2 (fr) * 1998-12-28 2000-07-05 Nikken Rentacom Company Limited Procédé de traitement pour la conservation de fleurs coupées
CN102885030A (zh) * 2012-10-29 2013-01-23 广州栋方日化有限公司 一种维持玫瑰花瓣在弱酸性或中性化妆品体系中稳定性的方法
CN103263372A (zh) * 2013-05-24 2013-08-28 苏州工业园区黎姿化妆品有限公司 一种天然花瓣和采用该天然花瓣的化妆品
US11998006B2 (en) 2017-07-07 2024-06-04 Embla Productions Hf. Method of tissue preservation

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GB2040669A (en) * 1979-01-30 1980-09-03 Univ Kingston Preservation of douglas fir needles
FR2589324A2 (fr) * 1985-08-02 1987-05-07 Sellegaard Laars Procede de conservation des vegetaux
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EP0279108A2 (fr) * 1986-10-23 1988-08-24 Marion A. Baker Procédé de conservation de végétaux ou de parties de ceux-ci
JPH0558801A (ja) * 1991-08-31 1993-03-09 Toshiyuki Sugino 乾燥押花の表面処理法及び表面処理液
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GB2040669A (en) * 1979-01-30 1980-09-03 Univ Kingston Preservation of douglas fir needles
FR2589324A2 (fr) * 1985-08-02 1987-05-07 Sellegaard Laars Procede de conservation des vegetaux
JPS62226901A (ja) * 1986-03-28 1987-10-05 Matsumura Sangyo Kk 化学的処理による植物措葉の作り方
EP0279108A2 (fr) * 1986-10-23 1988-08-24 Marion A. Baker Procédé de conservation de végétaux ou de parties de ceux-ci
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1016343A2 (fr) * 1998-12-28 2000-07-05 Nikken Rentacom Company Limited Procédé de traitement pour la conservation de fleurs coupées
EP1016343A3 (fr) * 1998-12-28 2000-11-15 Nikken Rentacom Company Limited Procédé de traitement pour la conservation de fleurs coupées
EP1290940A2 (fr) * 1998-12-28 2003-03-12 Nikken Rentacom Company Limited Procédé de traitement pour la conservation de fleurs coupées
EP1290940A3 (fr) * 1998-12-28 2003-08-20 Nikken Rentacom Company Limited Procédé de traitement pour la conservation de fleurs coupées
CN102885030A (zh) * 2012-10-29 2013-01-23 广州栋方日化有限公司 一种维持玫瑰花瓣在弱酸性或中性化妆品体系中稳定性的方法
CN103263372A (zh) * 2013-05-24 2013-08-28 苏州工业园区黎姿化妆品有限公司 一种天然花瓣和采用该天然花瓣的化妆品
US11998006B2 (en) 2017-07-07 2024-06-04 Embla Productions Hf. Method of tissue preservation

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