WO1999054490A3 - Plant-derived resistance gene - Google Patents

Plant-derived resistance gene Download PDF

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Publication number
WO1999054490A3
WO1999054490A3 PCT/GB1999/001182 GB9901182W WO9954490A3 WO 1999054490 A3 WO1999054490 A3 WO 1999054490A3 GB 9901182 W GB9901182 W GB 9901182W WO 9954490 A3 WO9954490 A3 WO 9954490A3
Authority
WO
WIPO (PCT)
Prior art keywords
resistance
plant
nucleic acids
resistance gene
disclosed
Prior art date
Application number
PCT/GB1999/001182
Other languages
French (fr)
Other versions
WO1999054490A2 (en
Inventor
Abdelhafid Bendahmane
David Charles Baulcombe
Konstantin Valerievich Kanyuka
Original Assignee
Plant Bioscience Ltd
Abdelhafid Bendahmane
David Charles Baulcombe
Konstantin Valerievich Kanyuka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd, Abdelhafid Bendahmane, David Charles Baulcombe, Konstantin Valerievich Kanyuka filed Critical Plant Bioscience Ltd
Priority to EP99917014A priority Critical patent/EP1078086A2/en
Priority to AU35296/99A priority patent/AU3529699A/en
Publication of WO1999054490A2 publication Critical patent/WO1999054490A2/en
Publication of WO1999054490A3 publication Critical patent/WO1999054490A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed are nucleic acids encoding polypeptides which are capable of conferring extreme resistance (ER) against, and being triggered by, plant pathogens such as viruses (e.g. PVX and related isolates). Preferred nucleic acids encode the Rx polynucleotide from Solanum tuberosum, or a variety of homologues (naturally occurring or derivatives) thereof, such as 111h1; 221h2; Ac15; Ac64; K39.hom. Particular methods of activating resistance by using combinations of resistance gene and elicitor are also disclosed, which in certain cases lead to a hypersensitive response. Further aspects of the invention include specific primers, vectors, host cells, polypeptides, antibodies and transgenic plants, plus methods of producing and employing these, in particular for influencing a resistance trait in a plant.
PCT/GB1999/001182 1998-04-16 1999-04-16 Plant-derived resistance gene WO1999054490A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99917014A EP1078086A2 (en) 1998-04-16 1999-04-16 Plant-derived resistance gene
AU35296/99A AU3529699A (en) 1998-04-16 1999-04-16 Plant-derived resistance gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9808083.1A GB9808083D0 (en) 1998-04-16 1998-04-16 Plant-derived resistance gene
GB9808083.1 1998-04-16

Publications (2)

Publication Number Publication Date
WO1999054490A2 WO1999054490A2 (en) 1999-10-28
WO1999054490A3 true WO1999054490A3 (en) 2000-01-06

Family

ID=10830437

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001182 WO1999054490A2 (en) 1998-04-16 1999-04-16 Plant-derived resistance gene

Country Status (4)

Country Link
EP (1) EP1078086A2 (en)
AU (1) AU3529699A (en)
GB (1) GB9808083D0 (en)
WO (1) WO1999054490A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8751898A (en) * 1998-07-31 2000-02-21 Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro - Dlo) Engineering nematode resistance in solanacae
GB9924483D0 (en) * 1999-10-15 1999-12-15 Plant Bioscience Ltd Modified resistance genes
CN1311078C (en) * 2002-03-27 2007-04-18 国立大学法人筑波大学 Novel meloidogyne-resistance gene and utilization thereof
EP1991681A1 (en) * 2006-02-28 2008-11-19 Keygene N.V. Disease resistant plants
US20150284796A1 (en) * 2014-04-03 2015-10-08 The Penn State Research Foundation Method to Identify Genes Relating to Improved Pathogen Resistance in Plants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995028478A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps2 gene and uses thereof
WO1998002545A2 (en) * 1996-07-11 1998-01-22 The Regents Of The University Of California Compositions and methods for plant pathogen resistance

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995028478A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps2 gene and uses thereof
WO1998002545A2 (en) * 1996-07-11 1998-01-22 The Regents Of The University Of California Compositions and methods for plant pathogen resistance

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BENDAHMANE ET AL: "high-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato", THEORETICAL AND APPLIED GENETICS, vol. 95, 1997, pages 153 - 162, XP002098294, ISSN: 0040-5752 *
DATABASE ENTREZ NCBI; 26 April 1996 (1996-04-26), GEOFFROY C ET AL.: "Paenibacillus alvei alveolysin (alv) gene", XP002120368 *
KANYUKA K ET AL.: "MAPPING OF INTRA LOCUS DUPLICATIONS AND INTROGRESSED DNA: AIDS TO MAP-BASED CLONING OF GENES FROM COMPLEX GENOMES ILLUSTRATED BY PHYSICAL ANALYSIS OF THE Rx LOCUS IN TETRAPLOID POTATO", THEORETICAL AND APPLIED GENETICS, vol. 98, no. 5, April 1999 (1999-04-01), pages 679 - 689, XP002120366 *
LONGSTAFF M ET AL: "EXTREME RESISTANCE TO POTATO VIRUS X INFECTION IN PLANTS EXPRESSING MODIFIED COMPONENT OF THE PUTATIVE VIRAL REPLICASE", EMBO JOURNAL, vol. 12, no. 2, 1 January 1993 (1993-01-01), pages 379 - 386, XP002006417, ISSN: 0261-4189 *
VANDERVOORT JR ET AL.: "TIGHT PHYSICAL LINKAGE OF THE NEMATODE RESISTANCE GENE GPA2 AND THE VIRUS RESISTANCE GENE Rx ON A SINGLE SEGMENT INTROGRESSED FROM THE WILD SPECIES Solanum tuberosum subsp andigena CPC1673 INTO CULTIVATED POTATO", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 12, no. 3, March 1999 (1999-03-01), pages 197 - 206, XP002120367 *

Also Published As

Publication number Publication date
GB9808083D0 (en) 1998-06-17
EP1078086A2 (en) 2001-02-28
AU3529699A (en) 1999-11-08
WO1999054490A2 (en) 1999-10-28

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