WO1999049024A3 - Methods and compositions for production of multimeric proteins in transgenic plants - Google Patents

Methods and compositions for production of multimeric proteins in transgenic plants Download PDF

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Publication number
WO1999049024A3
WO1999049024A3 PCT/US1999/006506 US9906506W WO9949024A3 WO 1999049024 A3 WO1999049024 A3 WO 1999049024A3 US 9906506 W US9906506 W US 9906506W WO 9949024 A3 WO9949024 A3 WO 9949024A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
production
methods
transgenic plants
plant cell
Prior art date
Application number
PCT/US1999/006506
Other languages
French (fr)
Other versions
WO1999049024A2 (en
WO1999049024A8 (en
WO1999049024A9 (en
Inventor
Keith L Wycoff
Sudhir K Jaiswal
Original Assignee
Planet Biotechnology Inc
Keith L Wycoff
Sudhir K Jaiswal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Planet Biotechnology Inc, Keith L Wycoff, Sudhir K Jaiswal filed Critical Planet Biotechnology Inc
Priority to AU31152/99A priority Critical patent/AU3115299A/en
Publication of WO1999049024A2 publication Critical patent/WO1999049024A2/en
Publication of WO1999049024A3 publication Critical patent/WO1999049024A3/en
Publication of WO1999049024A8 publication Critical patent/WO1999049024A8/en
Publication of WO1999049024A9 publication Critical patent/WO1999049024A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention is related to the field of methods and compositions for production of multimeric proteins in transgenic plants. The invention provides a method for producing a heterologous multimeric protein in a transgenic plant cell by transforming a plant cell with a plurality of naked plasmids. Each plasmid encodes less than all of the polypeptide components of the multimeric protein, and the plurality of transformed plasmids encodes all of the polypeptide components of the multimeric protein. The invention also provides a transgenic plant or plant cell expressing a multimeric protein that is heterologous to the plant cell.
PCT/US1999/006506 1998-03-25 1999-03-24 Methods and compositions for production of multimeric proteins in transgenic plants WO1999049024A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU31152/99A AU3115299A (en) 1998-03-25 1999-03-24 Methods and compositions for production of multimeric proteins in transgenic plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7924998P 1998-03-25 1998-03-25
US60/079,249 1998-03-25

Publications (4)

Publication Number Publication Date
WO1999049024A2 WO1999049024A2 (en) 1999-09-30
WO1999049024A3 true WO1999049024A3 (en) 1999-11-25
WO1999049024A8 WO1999049024A8 (en) 2000-03-02
WO1999049024A9 WO1999049024A9 (en) 2000-04-27

Family

ID=22149351

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/006506 WO1999049024A2 (en) 1998-03-25 1999-03-24 Methods and compositions for production of multimeric proteins in transgenic plants

Country Status (2)

Country Link
AU (1) AU3115299A (en)
WO (1) WO1999049024A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020168367A1 (en) * 2000-04-28 2002-11-14 Planet Biotechnology Incorporated Novel immunoadhesins for treating and preventing viral and bacterial diseases
DE60139864D1 (en) 2000-04-28 2009-10-22 Planet Biotechnology Inc IMMUNOADHESIN FOR VACCINATING RHINOVIRUS
BRPI0820042B1 (en) 2007-11-12 2020-05-19 North Carolina State University method of obtaining a tobacco plant, or cell or part thereof, having reduced levels of nornicotine, tobacco product, method of making a tobacco product, isolated polynucleotide, expression cassette, isolated polypeptide and method of obtaining a plant, or part of this plant, of the genus nicotiana
EP2791169B1 (en) 2011-12-16 2017-07-19 Synthon Biopharmaceuticals B.V. Compounds and methods for treating inflammatory diseases

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021012A1 (en) * 1994-12-30 1996-07-11 Planet Biotechnology, Inc. Methods for producing immunoglobulins containing protection proteins in plants and their use
WO1997042313A1 (en) * 1996-05-03 1997-11-13 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
WO1999007210A1 (en) * 1997-08-12 1999-02-18 North Carolina State University Genetically engineered duckweed

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021012A1 (en) * 1994-12-30 1996-07-11 Planet Biotechnology, Inc. Methods for producing immunoglobulins containing protection proteins in plants and their use
WO1997042313A1 (en) * 1996-05-03 1997-11-13 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
WO1999007210A1 (en) * 1997-08-12 1999-02-18 North Carolina State University Genetically engineered duckweed

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DE NEVE, M., ET AL.: "assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis", TRANSGENIC RESEARCH, vol. 2, 1993, pages 227 - 237, XP002116212 *
MA, J.K-C. AND HEIN, M.B.: "plant antibodies for immunotherapy", PLANT PHYSIOLOGY, vol. 109, 1995, pages 341 - 346, XP002116213 *

Also Published As

Publication number Publication date
WO1999049024A2 (en) 1999-09-30
AU3115299A (en) 1999-10-18
WO1999049024A8 (en) 2000-03-02
WO1999049024A9 (en) 2000-04-27

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