WO1999024621A3 - Methodes et compositions de detection de sequences nucleotidiques specifiques - Google Patents

Methodes et compositions de detection de sequences nucleotidiques specifiques Download PDF

Info

Publication number
WO1999024621A3
WO1999024621A3 PCT/US1998/024226 US9824226W WO9924621A3 WO 1999024621 A3 WO1999024621 A3 WO 1999024621A3 US 9824226 W US9824226 W US 9824226W WO 9924621 A3 WO9924621 A3 WO 9924621A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
detection
nucleic acid
invention comprises
Prior art date
Application number
PCT/US1998/024226
Other languages
English (en)
Other versions
WO1999024621A2 (fr
WO1999024621A9 (fr
Inventor
Elliot R Ramberg
Original Assignee
Cygene Inc
Elliot R Ramberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cygene Inc, Elliot R Ramberg filed Critical Cygene Inc
Priority to EP98959441A priority Critical patent/EP1030936A2/fr
Priority to CA002309861A priority patent/CA2309861A1/fr
Priority to KR1020007005156A priority patent/KR20010032036A/ko
Priority to MXPA00004675A priority patent/MXPA00004675A/es
Priority to AU15237/99A priority patent/AU1523799A/en
Priority to JP2000519613A priority patent/JP2001522588A/ja
Publication of WO1999024621A2 publication Critical patent/WO1999024621A2/fr
Publication of WO1999024621A3 publication Critical patent/WO1999024621A3/fr
Publication of WO1999024621A9 publication Critical patent/WO1999024621A9/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des méthodes et des compositions de détection de séquences nucléotidiques. Plus particulièrement, la présente invention concerne des méthodes et des compositions de détection de séquences génétiques spécifiques utilisant différentes stratégies de protection et de récupération de cibles nucléotidiques. De plus, la présente invention concerne de nouvelles méthodes de clivage d'acide nucléique. En outre, l'invention concerne des méthodes de détection de séquences nucléotidiques, lesquelles emploient des sondes de coupe non spécifiques de séquence, utilisent des formations triplex impliquant des structures en épingle à cheveux, et des méthodes d'amplification de signal.
PCT/US1998/024226 1997-11-12 1998-11-12 Methodes et compositions de detection de sequences nucleotidiques specifiques WO1999024621A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP98959441A EP1030936A2 (fr) 1997-11-12 1998-11-12 Methodes et compositions de detection de sequences nucleotidiques specifiques
CA002309861A CA2309861A1 (fr) 1997-11-12 1998-11-12 Methodes et compositions de detection de sequences nucleotidiques specifiques
KR1020007005156A KR20010032036A (ko) 1997-11-12 1998-11-12 특이적 뉴클레오타이드 서열의 검출방법 및 조성물
MXPA00004675A MXPA00004675A (es) 1997-11-12 1998-11-12 Metodos y composiciones para la deteccion de secuencias nucleotidicas especificas.
AU15237/99A AU1523799A (en) 1997-11-12 1998-11-12 Methods and compositions for detection of specific nucleotide sequences
JP2000519613A JP2001522588A (ja) 1997-11-12 1998-11-12 特異的ヌクレオチド配列を検出するための方法および組成物

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US6537897P 1997-11-12 1997-11-12
US7581298P 1998-02-24 1998-02-24
US7687298P 1998-03-05 1998-03-05
US60/075,812 1998-03-05
US60/076,872 1998-03-05
US60/065,378 1998-03-05

Publications (3)

Publication Number Publication Date
WO1999024621A2 WO1999024621A2 (fr) 1999-05-20
WO1999024621A3 true WO1999024621A3 (fr) 1999-08-19
WO1999024621A9 WO1999024621A9 (fr) 2001-05-31

Family

ID=27370777

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/024226 WO1999024621A2 (fr) 1997-11-12 1998-11-12 Methodes et compositions de detection de sequences nucleotidiques specifiques

Country Status (7)

Country Link
EP (1) EP1030936A2 (fr)
JP (1) JP2001522588A (fr)
KR (1) KR20010032036A (fr)
AU (1) AU1523799A (fr)
CA (1) CA2309861A1 (fr)
MX (1) MXPA00004675A (fr)
WO (1) WO1999024621A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100438174B1 (ko) * 2001-09-05 2004-07-01 엘지전자 주식회사 동기식 이동통신시스템에서의 데이터 전송속도 제어방법
GB2517700A (en) * 2013-08-27 2015-03-04 Lgc Ltd Oligonucleotides comprising a secondary structure and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015691A1 (fr) * 1995-10-27 1997-05-01 Ramberg Elliot R Procedes et compositions pour la detection de sequences specifiques de nucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015691A1 (fr) * 1995-10-27 1997-05-01 Ramberg Elliot R Procedes et compositions pour la detection de sequences specifiques de nucleotides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FRANCOIS J -C ET AL: "SEQUENCE-SPECIFIC RECOGNITION AND CLEAVAGE OF DUPLEX DNA VIA TRIPLE-HELIX FORMATION BY OLIGONUCLEOTIDES COVALENTLY LINKED TO A PHENANTHROLINE-COPPER CHELATE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 86, 1 December 1989 (1989-12-01), pages 9702 - 9706, XP000770860 *
MOSER H. & DERVAN P.: "Sequence-specific cleavage of double helical DNA by triple helix formation", SCIENCE, vol. 238, - 30 October 1987 (1987-10-30), pages 645 - 650, XP002104600 *
POVSIC T J ET AL: "TRIPLE HELIX FORMATION BY OLIGONUCLEOTIDES ON DNA EXTENDED TO THE PHYSIOLOGICAL PH RANGE", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 111, 1 January 1989 (1989-01-01), pages 3059 - 3061, XP000775708 *

Also Published As

Publication number Publication date
WO1999024621A2 (fr) 1999-05-20
KR20010032036A (ko) 2001-04-16
EP1030936A2 (fr) 2000-08-30
WO1999024621A9 (fr) 2001-05-31
MXPA00004675A (es) 2004-03-10
CA2309861A1 (fr) 1999-05-20
JP2001522588A (ja) 2001-11-20
AU1523799A (en) 1999-05-31

Similar Documents

Publication Publication Date Title
MX9803308A (es) Metodos y composiciones para la deteccion de secuencias especificas de nucleotidos.
WO2002000926A3 (fr) Diagnostic de maladies associees a une transduction de signal
WO2001081622A3 (fr) Diagnostic de maladies associees a la reparation de l'adn
WO1999049082A3 (fr) Nucleotides modifies et methodes utiles pour le sequençage d'acides nucleiques
WO2002057491A3 (fr) Caracterisation multiplex directe d'adn genomique
WO2002048402A3 (fr) Methodes et compositions de generation de copies multiples de sequences d'acides nucleiques et methodes de detection correspondantes
WO1999036516A3 (fr) Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote
AU2001245945A1 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
WO2002038806A3 (fr) Identification de polymorphismes d'acide nucleique
WO2002061133A3 (fr) Detection d'acide nucleique avec des sondes structurees
WO2001013086A3 (fr) Detection d'acides nucleiques
WO2004013357A3 (fr) Sequences nucleotidiques specifiques a francisella tularensis et methodes de detection de francisella tularensis
WO2002090581A3 (fr) Etiquette moleculaire pour surveiller un produit et procede l'utilisant
WO2001029222A3 (fr) Polypeptides ayant une activite phospholipase b et acides nucleiques codant ces derniers
WO2001047944A3 (fr) Acides nucleiques contenant des polymorphismes mononucleotidiques et procedes d'utilisation correspondants
WO1998026093A3 (fr) Formation en epingle a cheveux d'analogue de nucleotides fluorescents pour la detection de l'hybridation d'acides nucleiques
DK0805876T3 (da) Nukleinsyreamplifikationsoligonukleotider og prober til Lyme-sygdom-associeret Borrelia
WO2000029623A3 (fr) Acides nucleiques contenant des polymorphismes d'un seul nucleotide et utilisations de ces acides nucleiques
WO2002046454A3 (fr) Diagnostic de pathologies associees a l'angiogenese
HK1044965A1 (zh) T細胞受體Vβ-DβVβ序列和檢測它的方法
WO2000060113A3 (fr) Nouveau type de marqueur genetique a base de transposon
WO1999007896A3 (fr) Detection et confirmation de sequences nucleotidiques a l'aide d'oligonucleotides comprenant une sous-sequence s'hybridant exactement avec une sequence terminale connue et une sous-sequence s'hybridant avec une sequence non identifiee
WO1999024621A3 (fr) Methodes et compositions de detection de sequences nucleotidiques specifiques
WO1990009457A3 (fr) Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
AU2038500A (en) Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2309861

Country of ref document: CA

Kind code of ref document: A

Ref document number: 2309861

ENP Entry into the national phase

Ref document number: 2000 519613

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: PA/a/2000/004675

Country of ref document: MX

Ref document number: 1998959441

Country of ref document: EP

Ref document number: 1020007005156

Country of ref document: KR

Ref document number: 15237/99

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 1998959441

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1020007005156

Country of ref document: KR

AK Designated states

Kind code of ref document: C2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 7, 8, 10, 12, 17, 18, 20, 21, 26 AND 28-56, DESCRIPTION, REPLACED BY NEW PAGES 7, 8, 10, 12, 17, 18, 20, 21, 26 AND 28-57; PAGES 57 AND 58, CLAIMS, REPLACED BY NEW PAGES 58 AND 59; PAGES 1/16-16/16, DRAWINGS, REPLACED BY NEW PAGES 1/17-17/17; AFTER RECTIFICATION OF OBVIOUS ERRORS AS AUTHORIZED BY THE INTERNATIONAL SEARCHING AUTHORITY

WWW Wipo information: withdrawn in national office

Ref document number: 1998959441

Country of ref document: EP

WWR Wipo information: refused in national office

Ref document number: 1020007005156

Country of ref document: KR