WO1999011801A3 - Protease based gene switching system - Google Patents

Protease based gene switching system Download PDF

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Publication number
WO1999011801A3
WO1999011801A3 PCT/GB1998/002596 GB9802596W WO9911801A3 WO 1999011801 A3 WO1999011801 A3 WO 1999011801A3 GB 9802596 W GB9802596 W GB 9802596W WO 9911801 A3 WO9911801 A3 WO 9911801A3
Authority
WO
WIPO (PCT)
Prior art keywords
based gene
switching system
gene switching
protease based
protease
Prior art date
Application number
PCT/GB1998/002596
Other languages
French (fr)
Other versions
WO1999011801A2 (en
Inventor
Peter Michael Broad
Andrew David Charles
Melvyn Hollis
Linda Jean Maccallum
David John Scanlon
Original Assignee
Zeneca Ltd
Peter Michael Broad
Andrew David Charles
Melvyn Hollis
Linda Jean Maccallum
David John Scanlon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Peter Michael Broad, Andrew David Charles, Melvyn Hollis, Linda Jean Maccallum, David John Scanlon filed Critical Zeneca Ltd
Priority to EP98940421A priority Critical patent/EP1009835A2/en
Publication of WO1999011801A2 publication Critical patent/WO1999011801A2/en
Publication of WO1999011801A3 publication Critical patent/WO1999011801A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to materials and methods for protease-based gene switching systems, wherein a transcription factor is bound to a membrane via protease cleavage site. It also relates to the use of such materials and methods in the identification of substrates and inhibitors of proteases and in the design of altered specificity proteases.
PCT/GB1998/002596 1997-09-03 1998-08-28 Protease based gene switching system WO1999011801A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98940421A EP1009835A2 (en) 1997-09-03 1998-08-28 Protease based gene switching system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9718591.2 1997-09-03
GBGB9718591.2A GB9718591D0 (en) 1997-09-03 1997-09-03 Methods

Publications (2)

Publication Number Publication Date
WO1999011801A2 WO1999011801A2 (en) 1999-03-11
WO1999011801A3 true WO1999011801A3 (en) 1999-06-10

Family

ID=10818407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/002596 WO1999011801A2 (en) 1997-09-03 1998-08-28 Protease based gene switching system

Country Status (3)

Country Link
EP (1) EP1009835A2 (en)
GB (1) GB9718591D0 (en)
WO (1) WO1999011801A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10000161A1 (en) * 2000-01-06 2001-07-19 Boehringer Ingelheim Pharma Determining test substances that inhibit protease, involves incubating cells expressing fusion protein having substrate with cleavage site for protease and reporter, measuring cleaved reporter and comparing with standard
WO2002024899A2 (en) * 2000-09-25 2002-03-28 Valentis, Inc. Improved system for regulation of transgene expression
DE10211063A1 (en) 2002-03-13 2003-10-09 Axaron Bioscience Ag New methods for the detection and analysis of protein interactions in vivo
EP1361284A1 (en) * 2002-05-10 2003-11-12 Direvo Biotech AG Process for generating sequence-specific proteases by directed evolution and use thereof
FR2849853A1 (en) * 2003-01-15 2004-07-16 Millegen New fusion protein for inducing expression of a gene, useful particularly for regulating gene therapy, also in screening for active agents, comprises nucleic acid-binding, expression-activating and membrane-binding domains
US7935788B2 (en) 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
WO2006037215A1 (en) 2004-10-04 2006-04-13 National Research Council Of Canada Expression system, components thereof and methods of use
MX2011014040A (en) 2009-06-19 2012-06-01 Medimmune Llc Protease variants.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0355737A2 (en) * 1988-08-24 1990-02-28 BEHRINGWERKE Aktiengesellschaft Expression of functional homologous and heterologous proteins on the outer membrane of E.coli and other gram-negative bacteria

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0355737A2 (en) * 1988-08-24 1990-02-28 BEHRINGWERKE Aktiengesellschaft Expression of functional homologous and heterologous proteins on the outer membrane of E.coli and other gram-negative bacteria

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HIROWATARI Y ET AL: "A novel method for analysis of viral proteinase activity encoded by hepatitus C virus in cultured cells", ANALYTICAL BIOCHEMISTRY, vol. 225, 1995, pages 113 - 120, XP002096985 *
KUMAGAI H ET AL: "Molecular dissection of the role of the membrane domain in the regulated degradation of 3-hydroxy-3- methylglutaryl coenzyme A reductase.", JOURNAL OF BIOLOGICAL CHEMISTRY, (1995 AUG 11) 270 (32) 19107-13. JOURNAL CODE: HIV. ISSN: 0021-9258., United States, XP002096987 *
SMITH T A ET AL: "Direct selection for sequences encoding proteases of known specificity.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (1991 JUN 15) 88 (12) 5159-62. JOURNAL CODE: PV3. ISSN: 0027-8424., United States, XP002096986 *

Also Published As

Publication number Publication date
WO1999011801A2 (en) 1999-03-11
GB9718591D0 (en) 1997-11-05
EP1009835A2 (en) 2000-06-21

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