WO1999011763A3 - Method for purifying and concentrating aav-2 and antigen portions thereof - Google Patents

Method for purifying and concentrating aav-2 and antigen portions thereof Download PDF

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Publication number
WO1999011763A3
WO1999011763A3 PCT/DE1998/002569 DE9802569W WO9911763A3 WO 1999011763 A3 WO1999011763 A3 WO 1999011763A3 DE 9802569 W DE9802569 W DE 9802569W WO 9911763 A3 WO9911763 A3 WO 9911763A3
Authority
WO
WIPO (PCT)
Prior art keywords
aav
purifying
antigen portions
concentrating
antigen
Prior art date
Application number
PCT/DE1998/002569
Other languages
German (de)
French (fr)
Other versions
WO1999011763A9 (en
WO1999011763A2 (en
Inventor
Juergen Kleinschmidt
Andrea Kern
Original Assignee
Deutsches Krebsforsch
Juergen Kleinschmidt
Andrea Kern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforsch, Juergen Kleinschmidt, Andrea Kern filed Critical Deutsches Krebsforsch
Priority to EP98951251A priority Critical patent/EP1009809A2/en
Publication of WO1999011763A2 publication Critical patent/WO1999011763A2/en
Publication of WO1999011763A3 publication Critical patent/WO1999011763A3/en
Publication of WO1999011763A9 publication Critical patent/WO1999011763A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a method for purifying and concentrating AAV-2 and antigen portions thereof. AAV-2 or antigen portions thereof are bonded to an activated chromatographic material on which antibodies are linked, said antibodies being directed against AAV-2. Afterwards, elution using a solution containing 0.5 to 4.5 MgCl2 is carried out. The invention also relates to a kit for implementing said method.
PCT/DE1998/002569 1997-09-02 1998-09-01 Method for purifying and concentrating aav-2 and antigen portions thereof WO1999011763A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98951251A EP1009809A2 (en) 1997-09-02 1998-09-01 Method for purifying and concentrating aav-2 and antigen portions thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19738292.4 1997-09-02
DE1997138292 DE19738292C1 (en) 1997-09-02 1997-09-02 Process for the purification and concentration of AAV-2 and antigenic parts thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09508037 A-371-Of-International 2000-06-23
US09/886,144 Continuation US20010031463A1 (en) 1997-09-02 2001-06-20 Method for purifying and concentrating AAV-2 and antigen portions thereof

Publications (3)

Publication Number Publication Date
WO1999011763A2 WO1999011763A2 (en) 1999-03-11
WO1999011763A3 true WO1999011763A3 (en) 1999-05-14
WO1999011763A9 WO1999011763A9 (en) 1999-06-17

Family

ID=7840939

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/002569 WO1999011763A2 (en) 1997-09-02 1998-09-01 Method for purifying and concentrating aav-2 and antigen portions thereof

Country Status (3)

Country Link
EP (1) EP1009809A2 (en)
DE (1) DE19738292C1 (en)
WO (1) WO1999011763A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995011997A1 (en) * 1993-10-28 1995-05-04 Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts Adeno-associated virus - its diagnostic use with early abortion
WO1997008298A1 (en) * 1995-08-30 1997-03-06 Genzyme Corporation Chromatographic purification of adenovirus and aav

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995011997A1 (en) * 1993-10-28 1995-05-04 Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts Adeno-associated virus - its diagnostic use with early abortion
WO1997008298A1 (en) * 1995-08-30 1997-03-06 Genzyme Corporation Chromatographic purification of adenovirus and aav

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
D. GRIMM ET AL.: "Novel tools for production and purification of recombinant adenoassociated virus vectors.", HUMAN GENE THERAPY, vol. 9, no. 18, 10 December 1998 (1998-12-10), New York, NY, VSA, pages 2745 - 2760, XP002093963 *
K. DURKEE ET AL.: "Immunoaffinity chromatographic purification of Russell's viper venom Factor X activator using elution in high concentrations of magnesium chloride.", PROTEIN EXPRESSION AND PURIFICATION, vol. 4, no. 5, October 1993 (1993-10-01), pages 405 - 411, XP002093962 *
M. HESSING ET AL.: "Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells.", JOURNAL OF CHROMATOGRAPHY, vol. 599, no. 1-2, 22 May 1992 (1992-05-22), Amsterdam, Niederlande, pages 267 - 272, XP002093960 *
P. O'NEIL ET AL.: "Virus harvesting and affinity-based liquid chromatography.", BIO/TECHNOLOGY, vol. 11, no. 2, February 1993 (1993-02-01), Basingstoke, Grossbritannien, pages 173 - 178, XP000195205 *
T. SUGIURA ET AL.: "Purification of equine infectious anemia virus antigen by affinity chromatography.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 5, no. 6, June 1977 (1977-06-01), Washington, DC, VSA, pages 635 - 639, XP002093961 *

Also Published As

Publication number Publication date
WO1999011763A9 (en) 1999-06-17
EP1009809A2 (en) 2000-06-21
WO1999011763A2 (en) 1999-03-11
DE19738292C1 (en) 1999-05-12

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