WO1999002646A1 - Self contained cell growth system - Google Patents
Self contained cell growth system Download PDFInfo
- Publication number
- WO1999002646A1 WO1999002646A1 PCT/US1998/014258 US9814258W WO9902646A1 WO 1999002646 A1 WO1999002646 A1 WO 1999002646A1 US 9814258 W US9814258 W US 9814258W WO 9902646 A1 WO9902646 A1 WO 9902646A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tank
- cell growth
- media
- plates
- reservoir tank
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Definitions
- FIG. 1 A first figure.
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present application relates generally to a system (1) for the practice of high density cell culture. The system (1) employs the use of two tanks; a cell growth tank (14) having multiple cell growth chambers, is preferably situated within a reservoir tank (12). A series of pumps (82) and outlets (80) provide an even and continuous circulation of media and air to the system and between tanks. The system is designed to operate with minimal supervision and constantly over long periods of time.
Description
Claims
1. A tissue culture device comprising: a reservoir tank capable of containing fluid; a cell growth tank situated within said reservoir tank having a plurality of cell growth chambers, the interior surfaces of said growth chamber.; adapted for the growth of cells; an inlet conduit for providing a source of fluid to the chambers; an outlet conduit adapted to be in fluid communication with fluid in said reservoir tank; and a circulation pump connecting said inlet conduit to said fluid in said reservoir tank.
2. A tissue culture device as claimed in claim 1 wherein the cell growth chambers are defined by the spacings between a plurality of stacked plates.
3. A tissue culture device as claimed in claim 1 further comprising an inlet through which fluid from an external source may be pumped into said reservoir tank.
4. The tissue culture device of claim 3 further comprising a sipper tube extending into said reservoir tank to a predetermined depth, said sipper tube connected to an external pump capable of drawing fluid from said reservoir.
5. The tissue culture device of claim 4 further comprising at least one air inlet in said reservoir tank capable of attachment to a pump through which said pump delivers air into said reservoir tank; and at least one air outlet in said reservoir tank capable of venting air from within said reservoir tank, whereby an air flow is created between said air inlet and said air outlet by differential pressure.
6. The tissue culture device of claim 5 further comprising a media spreading oxygenator nozzle having cylindrical side walls having at least one aperture therein, a
13
solid bottom, and an open top, said nozzle extending into said reservoir tank, whereby said outlet conduit is fluidly attached to said top of said oxygenator nozzle.
7. The tissue culture device of claim 1 wherein a removable lid forms a top surface of said cell growth tank.
8. The tissue culture device of claim 6 further comprising a curtain suspended within said reservoir tank and substantially extending the length of the tank.
9. The tissue culture device of claim 1 wherein said reservoir tank and said cell growth tank are formed of a transparent material.
10. The tissue culture device of claim 1 further comprising passages for providing fluid communication between said inlet conduit and each of said growth chambers.
11. The tissue culture device of claim 1 wherein said inlet conduit is fluidly attached to a trough running longitudinally along a bottom wall of said cell growth tank.
12. The tissue culture device of claim 1 wherein said reservoir tank and said cell growth tank are optically clear.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98935588A EP0994941B1 (en) | 1997-07-11 | 1998-07-07 | Self contained cell growth system |
JP2000502147A JP2001509374A (en) | 1997-07-11 | 1998-07-07 | Built-in cell growth system |
DE69831948T DE69831948T2 (en) | 1997-07-11 | 1998-07-07 | INDEPENDENT CELL GROWTH SYSTEM |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US5231197P | 1997-07-11 | 1997-07-11 | |
US60/052,311 | 1997-07-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999002646A1 true WO1999002646A1 (en) | 1999-01-21 |
Family
ID=21976767
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/014258 WO1999002646A1 (en) | 1997-07-11 | 1998-07-07 | Self contained cell growth system |
Country Status (5)
Country | Link |
---|---|
US (1) | US6107085A (en) |
EP (1) | EP0994941B1 (en) |
JP (1) | JP2001509374A (en) |
DE (1) | DE69831948T2 (en) |
WO (1) | WO1999002646A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003064586A2 (en) * | 2002-01-28 | 2003-08-07 | Probiogen Ag | Method and device for cultivating cells in high densities and for obtaining products from said cells |
US9005550B2 (en) | 2012-10-29 | 2015-04-14 | Corning Incorporated | Multi-layered cell culture vessel with manifold grips |
CN106701542A (en) * | 2017-03-22 | 2017-05-24 | 洛阳师范学院 | Multifunctional culture device for bacteria biofilm |
US9976114B2 (en) | 2009-07-22 | 2018-05-22 | Corning Incorporated | Multilayer tissue cell culture vessel |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10016554A1 (en) * | 2000-04-03 | 2001-10-18 | Rootec Ges Fuer Bioaktive Wirk | Plant or animal tissue cultivation unit, comprises fermenter container, supply for liquid nutrients and gases, discharger for used nutrients, and carrier plates |
US6569675B2 (en) | 2000-06-16 | 2003-05-27 | Corning Incorporated | Cell cultivating flask and method for using the cell cultivating flask |
GB0121735D0 (en) * | 2001-09-10 | 2001-10-31 | Deblois Michel | Bioreactor for the treatment of organic and inorganic waste |
US6943009B2 (en) | 2002-05-15 | 2005-09-13 | Corning Incorporated | Multi-well assembly for growing cultures in-vitro |
US8323984B2 (en) * | 2002-12-19 | 2012-12-04 | Beckman Coulter, Inc. | Method and apparatus for mixing blood samples for cell analysis |
EP1691727B1 (en) | 2003-12-11 | 2011-07-06 | Isto Technologies Inc. | Particulate cartilage system |
US9354156B2 (en) | 2007-02-08 | 2016-05-31 | Emd Millipore Corporation | Microfluidic particle analysis method, device and system |
US8257964B2 (en) * | 2006-01-04 | 2012-09-04 | Cell ASIC | Microwell cell-culture device and fabrication method |
US9260688B2 (en) | 2005-07-07 | 2016-02-16 | The Regents Of The University Of California | Methods and apparatus for cell culture array |
US9388374B2 (en) | 2005-07-07 | 2016-07-12 | Emd Millipore Corporation | Microfluidic cell culture systems |
US9637715B2 (en) | 2005-07-07 | 2017-05-02 | Emd Millipore Corporation | Cell culture and invasion assay method and system |
US9376658B2 (en) | 2008-01-03 | 2016-06-28 | Emd Millipore Corporation | Cell culture array system for automated assays and methods of operation and manufacture thereof |
US7745209B2 (en) | 2005-07-26 | 2010-06-29 | Corning Incorporated | Multilayered cell culture apparatus |
US20070048848A1 (en) * | 2005-08-25 | 2007-03-01 | Sunsource Industries | Method, apparatus and system for biodiesel production from algae |
EP1916964A4 (en) | 2005-08-26 | 2015-11-04 | Zimmer Inc | Implants and methods for repair, replacement and treatment of joint disease |
EP1764117A1 (en) * | 2005-09-20 | 2007-03-21 | Zimmer GmbH | Implant for the repair of a cartilage defect and method for manufacturing the implant |
US8272255B2 (en) | 2006-05-22 | 2012-09-25 | 3M Innovative Properties Company | System and method for preparing samples |
US7745210B2 (en) * | 2006-06-30 | 2010-06-29 | Corning Incorporated | Fluid flow diverter for cell culture vessel |
US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
US20080206845A1 (en) * | 2007-02-23 | 2008-08-28 | Emilio Barbera-Guillem | Bioreactor analysis system |
US7897379B2 (en) * | 2007-02-26 | 2011-03-01 | Corning Incorporated | Device and method for reducing bubble formation in cell culture |
WO2008112845A2 (en) * | 2007-03-14 | 2008-09-18 | Caridianbct, Inc. | Cell expansion apparatus with plate bioreactor |
EP2134297B1 (en) | 2007-04-12 | 2017-03-08 | Zimmer, Inc. | Apparatus for tissue repair |
US9309491B2 (en) * | 2007-05-29 | 2016-04-12 | Corning Incorporated | Cell culture apparatus for co-culture of cells |
EP2214830B1 (en) * | 2007-11-20 | 2012-09-12 | 3M Innovative Properties Company | Sample preparation container and method |
WO2009067503A1 (en) * | 2007-11-20 | 2009-05-28 | 3M Innovative Properties Company | Sample preparation for environmental sampling |
WO2009067513A1 (en) | 2007-11-20 | 2009-05-28 | 3M Innovative Properties Company | Sample preparation container and method |
JP2011505550A (en) | 2007-11-20 | 2011-02-24 | スリーエム イノベイティブ プロパティズ カンパニー | System and method for preparing and collecting samples |
EP2933325B1 (en) * | 2008-01-25 | 2019-09-25 | Corning Incorporated | Manifold for limited access multi-layer cell culture system |
ES2733631T3 (en) * | 2008-07-16 | 2019-12-02 | Emd Millipore Corp | A multi-level cell culture system |
US9353342B2 (en) | 2010-01-21 | 2016-05-31 | Emd Millipore Corporation | Cell culture and gradient migration assay methods and devices |
US10526572B2 (en) | 2011-04-01 | 2020-01-07 | EMD Millipore Corporaticn | Cell culture and invasion assay method and system |
SG11201402558QA (en) | 2011-12-03 | 2014-06-27 | Emd Millipore Corp | Micro-incubation systems for microfluidic cell culture and methods |
JP5912734B2 (en) * | 2012-03-26 | 2016-04-27 | テルモ株式会社 | Cell culture equipment |
US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
WO2016022722A1 (en) | 2014-08-05 | 2016-02-11 | The Charles Stark Draper Laboratory, Inc. | Fluid circulation systems incorporating fluid leveling devices |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3853712A (en) * | 1971-02-09 | 1974-12-10 | Nat Res Dev | Cell culture systems |
US4225671A (en) * | 1977-06-10 | 1980-09-30 | Battelle-Institut E.V. | Process for the in-vitro biosynthesis of hormones, especially insulin |
US4228243A (en) * | 1978-07-13 | 1980-10-14 | Toray Industries, Inc. | Cell culture propagation apparatus |
US4683062A (en) * | 1984-11-30 | 1987-07-28 | Ceskoslovenska Akademie Ved | Method of circulation of a liquid phase through a solid phase particularly for biocatalytical reactions and a device for realization thereof |
JPS63287477A (en) * | 1987-05-20 | 1988-11-24 | Ngk Insulators Ltd | Fermentation by immobilized microorganism and apparatus therefor |
US5100801A (en) * | 1989-01-26 | 1992-03-31 | Biocontrol Systems, Inc. | Device for sequential microbial enrichment in a single apparatus |
US5240854A (en) * | 1989-06-05 | 1993-08-31 | Berry Eric S | Continuous high-density cell culture system |
US5658797A (en) * | 1992-03-03 | 1997-08-19 | Bader; Augustinus | Device for the treatment of cell cultures |
US5763267A (en) * | 1996-04-16 | 1998-06-09 | Advanced Tissue Sciences | Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures |
US5766949A (en) * | 1996-06-18 | 1998-06-16 | Ming-Yi Liau | Method and apparatus for cultivating anchorage dependent monolayer cells |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4668632A (en) * | 1985-02-06 | 1987-05-26 | Vxr, Inc. | Sparger and apparatus for and method of growing cells |
US5026649A (en) * | 1986-03-20 | 1991-06-25 | Costar Corporation | Apparatus for growing tissue cultures in vitro |
DE3612453A1 (en) * | 1986-04-14 | 1987-10-15 | Diessel Gmbh & Co | DEVICE FOR CULTIVATING CELL CULTURES |
US4734373A (en) * | 1986-06-24 | 1988-03-29 | Bartal Arie H | Apparatus for enhancing cell growth, preservation and transport |
US5262320A (en) * | 1990-06-18 | 1993-11-16 | Massachusetts Institute Of Technology | Cell-culturing apparatus and method employing a macroporous support |
JPH0728722B2 (en) * | 1990-06-29 | 1995-04-05 | 富士写真フイルム株式会社 | Bioreactor equipment |
US5316945A (en) * | 1991-12-14 | 1994-05-31 | Will Minuth | Cell carrier arrangement |
JPH0870844A (en) * | 1994-08-31 | 1996-03-19 | Akira Tokishita | Culture apparatus for useful microorganism |
US5585266A (en) * | 1995-10-05 | 1996-12-17 | Plitt; Cheryl A. | Immobilized cell bioreactor |
-
1998
- 1998-07-03 US US09/110,754 patent/US6107085A/en not_active Expired - Lifetime
- 1998-07-07 EP EP98935588A patent/EP0994941B1/en not_active Expired - Lifetime
- 1998-07-07 JP JP2000502147A patent/JP2001509374A/en not_active Withdrawn
- 1998-07-07 DE DE69831948T patent/DE69831948T2/en not_active Expired - Fee Related
- 1998-07-07 WO PCT/US1998/014258 patent/WO1999002646A1/en active IP Right Grant
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3853712A (en) * | 1971-02-09 | 1974-12-10 | Nat Res Dev | Cell culture systems |
US4225671A (en) * | 1977-06-10 | 1980-09-30 | Battelle-Institut E.V. | Process for the in-vitro biosynthesis of hormones, especially insulin |
US4228243A (en) * | 1978-07-13 | 1980-10-14 | Toray Industries, Inc. | Cell culture propagation apparatus |
US4683062A (en) * | 1984-11-30 | 1987-07-28 | Ceskoslovenska Akademie Ved | Method of circulation of a liquid phase through a solid phase particularly for biocatalytical reactions and a device for realization thereof |
JPS63287477A (en) * | 1987-05-20 | 1988-11-24 | Ngk Insulators Ltd | Fermentation by immobilized microorganism and apparatus therefor |
US5100801A (en) * | 1989-01-26 | 1992-03-31 | Biocontrol Systems, Inc. | Device for sequential microbial enrichment in a single apparatus |
US5240854A (en) * | 1989-06-05 | 1993-08-31 | Berry Eric S | Continuous high-density cell culture system |
US5658797A (en) * | 1992-03-03 | 1997-08-19 | Bader; Augustinus | Device for the treatment of cell cultures |
US5763267A (en) * | 1996-04-16 | 1998-06-09 | Advanced Tissue Sciences | Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures |
US5766949A (en) * | 1996-06-18 | 1998-06-16 | Ming-Yi Liau | Method and apparatus for cultivating anchorage dependent monolayer cells |
Non-Patent Citations (1)
Title |
---|
See also references of EP0994941A4 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003064586A2 (en) * | 2002-01-28 | 2003-08-07 | Probiogen Ag | Method and device for cultivating cells in high densities and for obtaining products from said cells |
WO2003064586A3 (en) * | 2002-01-28 | 2004-03-25 | Probiogen Ag | Method and device for cultivating cells in high densities and for obtaining products from said cells |
US9976114B2 (en) | 2009-07-22 | 2018-05-22 | Corning Incorporated | Multilayer tissue cell culture vessel |
US10053661B2 (en) | 2009-07-22 | 2018-08-21 | Corning Incorporated | Multilayer tissue culture vessel |
US10155924B2 (en) | 2009-07-22 | 2018-12-18 | Corning Incorporated | Multilayer tissue culture vessel |
US9005550B2 (en) | 2012-10-29 | 2015-04-14 | Corning Incorporated | Multi-layered cell culture vessel with manifold grips |
CN106701542A (en) * | 2017-03-22 | 2017-05-24 | 洛阳师范学院 | Multifunctional culture device for bacteria biofilm |
Also Published As
Publication number | Publication date |
---|---|
DE69831948D1 (en) | 2005-11-24 |
DE69831948T2 (en) | 2006-07-27 |
EP0994941B1 (en) | 2005-10-19 |
JP2001509374A (en) | 2001-07-24 |
EP0994941A4 (en) | 2004-10-20 |
EP0994941A1 (en) | 2000-04-26 |
US6107085A (en) | 2000-08-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6107085A (en) | Self contained cell growth system | |
US20230265383A1 (en) | Highly efficient gas permeable devices and methods for culturing cells | |
US4783258A (en) | Filter cartridge with reuseable framework | |
US5310676A (en) | Cell cultivating device | |
US5139951A (en) | Culture device having a detachable cell or tissue growth surface | |
US5053125A (en) | Filter cartridge | |
JPS59175877A (en) | Culturing method and system | |
CN109689853A (en) | Bioreactor | |
WO2015008949A1 (en) | Simple animal cell incubator and method for incubating animal cells using same | |
KR20180117174A (en) | Cell culture vessel and method of use thereof | |
EP0667894B1 (en) | A cell cultivating device | |
EP0500549B1 (en) | Continuous high-density cell culture system | |
JPH04262775A (en) | Continuous cell cultivating device | |
JP2585866B2 (en) | Continuous high density cell culture system | |
KR101634710B1 (en) | Plankton cultureing apparatus | |
CN210987729U (en) | Novel three-dimensional cultivation box | |
CA1220389A (en) | Apparatus for incubating fish eggs | |
AU2013200127A2 (en) | Highly efficient gas permeable devices and methods for culturing cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 1998935588 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1998935588 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 1998935588 Country of ref document: EP |