WO1998053709A1 - Formulas de aminoacidos para personas de la tercera edad y procedimiento para el calculo de las mismas - Google Patents
Formulas de aminoacidos para personas de la tercera edad y procedimiento para el calculo de las mismas Download PDFInfo
- Publication number
- WO1998053709A1 WO1998053709A1 PCT/ES1998/000149 ES9800149W WO9853709A1 WO 1998053709 A1 WO1998053709 A1 WO 1998053709A1 ES 9800149 W ES9800149 W ES 9800149W WO 9853709 A1 WO9853709 A1 WO 9853709A1
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- WIPO (PCT)
- Prior art keywords
- amino acid
- amino acids
- diet
- day
- μmol
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/415—1,2-Diazoles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
Definitions
- the present invention relates to amino acid formulas in artificial diets aimed at the elderly. Similarly, the present invention relates to a method for calculating the concentration of each amino acid necessary to be administered in both parenteral and enteral nutrition, as well as to the therapeutic use of amino acid formulas in artificial diets in people of the seniors
- the present invention provides a new method for calculating the necessary concentration of each amino acid to be administered. in an artificial diet for the elderly according to the intracellular needs of each amino acid, likewise the object of the present invention is the amino acid formulas of the enteral and parenteral artificial diets for the elderly, understanding as the amino acid formula the proportion of the various amino acids in the diet
- amino acid formulas object of the invention, are calculated from the Transformation Factor and the size of the USEFUL POOL of each amino acid.
- the Transformation Factor is obtained by relating the amount of amino acid administered during a certain time and the arterial concentration of amino acids after that period
- the present invention provides new amino acid formulas for the elderly characterized by every 100 g of total amino acids contain 11 to 17 g of L-glutamic acid; from 6 to 8 g of L-serine; from 4 to 10 g of glycine; from 2 to 3.50 g of L-histidine; from 3.50 to 5 g of L-threonine; from 5 to 6.50 g of L-alanine; from 4.50 to 7 g of L-arginine; from 8.25 to 10 g of L-proline; from 1.50 to 4.50 g of L-tyrosine; from 5.50 to 7 g of L-valine; from 2 to 3.50 g of L-methionine; from 0.25 to 2.25 g of L-cysteine; from 5.75 to 7.25 g of L-isoleucine; from 9 to 10.75 g of L-leucine; from 4 to 6 g of L-phenylalanine; from 1 to 2.50 g of L-tryptophan; from 7
- the present invention also provides a new method for calculating amino acid formulas, that is, the necessary concentration of each amino acid to be administered in an artificial diet.
- Preferred amino acid formulas are those in which per 100 g of total amino acids contain: 11 to 12 g of L-glutamic acid; from 6.50 to 7.75 g of L-serine; from 8.75 to 9.75 g of glycine; from 2.50 to 3.50 g of L-histidine; from 3.75 to 4.80 g of L-threonine; 5 50 to 6 50 g of L-alanine, 5 75 to 7 g of L-arginine, 8 75 to 10 g of L-prohna, 1 50 to 2 75 g of L-tyrosine, 5 80 to 7 g of L-valine, 2 25 to 3 25 g of L-methionine, 0 25 to 1 50 g of L-cysteine, 6 to 7 g of L-isoleucine, 9 50 to 10 60 g of L -leucma of 4 to 5 g of L-phenylalanine, of 1 25 to 2 30 g of L-t-tryptophan,
- amino acid formulas in which per 100 g of total amino acids contain: 11.2 to 11.6 g of L-glutamic acid; from 7 to 7.4 g of L-serine; from 9 to 9.4 g of glycine; from 2.8 to 3.2 g of L-histidine; from 4J to 4.5 g of L-threonine; from 5.8 to 6.2 g of L-alanine; from 6J to 6.5 g of L-arginine; 9.2 to 9.6 g of L-proline; from 1.9 to 2.3 g of L-tyrosine; from 6.2 to 6.6 g of L-valine; 2.5 to 2.9 g of L-methionine; 0.6 to 1 g of L-cysteine; 6.4 to 6.8 g of L-isoleucine; from 9.9 to 10.3 g of L-leucine; from 4.3 to 4.7 g of L-phenylalanine; 1.6 to 2 g of L-tryptophan;
- a second object of the present invention is to provide a new method for calculating amino acid formulas
- the amino acid formulas that is, the necessary concentration of each amino acid to be administered in a Artificial diet for elderly people, are obtained through a procedure in which old animals are used, as described below
- AA means amino acid
- Factors the Transformation Factor and [Artenial AA or in tefidojes the size of the USEFUL POOL of each amino acid in arterial blood or tissue
- the amino acid formula in% (grams of free amino acids / 100 grams of total amino acids) can be obtained in the Parenteral or Enteral Base Diet From the Amino Acid Formula thus calculated, the final Amino Acid Formula can be established taking into account, for example, studies on the stability of amino acid solutions, or the solubility of amino acids if it is a Parenteral Nutrition, and the profile of amino acids of the proteins available in the market, in the case of Whole Nutrition
- the useful POOL of amino acids is determined on the basis of experiments that, although it would be best to perform them directly on human beings, in practice they are developed, for ethical reasons, on animals of the so-called laboratory, such as rats or similar. that the requirements and metabolism of amino acids among mammals do not show consistent differences, we can extrapolate the results obtained in experiments on animals to humans (see, for example, the article by HN Munro, "Evolution of protein metabolism m mammals", published in Mammahan protein metabolism, vol III, pp. 133-182, edited by HN Munro, Academic Press, New York, 1969)
- the USEFUL POOL of amino acids which is based on the identification of the amount of each amino acid (size of the amino acid POOL) that is not contaminated by the effect of the nutrition received during the previous days, can be calculated in turn from from the extrapolation at zero time of the average of the values of the amino acid concentrations obtained after subjecting old animals, for example male Wistar rats 18 to 30 months of age, to fasting between one day and the time in which they usually survive the kind of animal used, so when it comes to Wistar rats the maximum duration of fasting is set to 20 days
- blood samples can be obtained by puncture under anesthesia, for example from the carotid artery, or once the animal has been sacrificed, it can extract various tissues such as liver, heart, kidney or jejunum.
- arterial blood since it is readily available and remains invariably invariable by malnutrition (fasting) Fasting alters the flow of arterial amino acids in a particular tissue, but maintains the blood flow invariably after administration of nutrition amino acid flows of arterial blood is modify
- the USEFUL POOL of each amino acid is calculated from the average of the values of the amino acid concentrations extrapolated at zero time plus two standard deviations (2SD).
- the Transformation Factor necessary to calculate the Amino Acid Formula can be obtained in turn relating the amount of amino acid administered for one to 5 days, for example 24 hours in the case of a parenteral nutrition diet or 4 days when Try an enteral nutrition diet and the arterial concentration of amino acids after that period:
- Example 1 Amino Acid Formula in Parenteral Nutrition Solutions. Use of arterial blood for determination.
- Rats 48 male Wistar rats of 501-923 g and 24 months old, considered old rats according to international standards, are randomly distributed in 8 groups of 6 animals. Rats are fasting 1, 3, 5, 7, 11, 13 and 15 days respectively. The 2-year-old rats survive fasting for 15 to 20 days. However, the maximum duration of fasting is set to 15 days, so most rats survive until the day of sacrifice.
- HPLC high performance liquid chromatography
- PITC phenylisothiocyanate
- the amount of sample injected it is 10 ⁇ l, using an automatic injector (Waters 700)
- the characteristics of the column (PICO-TAG R Thousand for physiological amino acids) are 30 cm x 46 mm, C 18 (5 ⁇ m)
- the solvent system used consists of in a mixture of two diluents (A) an aqueous sodium acetate buffer pH 6 35, and (B) a mixture of acetonityl, methanol and water
- the internal standard used is methioninsulfone
- the calibration curve includes the following amino acid concentrations 25, 50, 100, 200, 400 and 600 ⁇ mol / l
- the analysis temperature is 46 ° C
- the duration of the chromatogram is 90 minutes. The system is then equilibrated until the next injection.
- the concentration of amino acids in the different tissues can correlate with the fasting period in a significant way or not. Although the lack of correlation may make it difficult to calculate the theoretical amino acid concentration on the zero day of fasting as a value to be used as a starting point for the initial formulation of the diet, the calculated "a" can be estimated - ordered at the origin - (or even the average of the values obtained on the 15th day of fasting)
- a group of amino acids decrease their value throughout the study period in a statistically significant way (for example, the amino acid L-typtophan in arterial blood, see figure 1)
- the coefficient "a" ( ⁇ M amino acid on day zero) is used to define the maximum expansion of the size of the USEFUL POOL for each amino acid in the particular organ studied, in this case the arterial blood and the values obtained on the 15 day fasting the minimum expansion of the USEFUL POOL
- the minimum expansion of the USEFUL POOL is calculated from the average of values obtained on the 15th day of fasting, whose value is 0 ⁇ mol / l
- the average of the values found on the 15th day of fasting is identified as the maximum expansion of the size of the USEFUL POOL for each amino acid in the studied organ, in this case arterial blood, and the coefficient "a" ( ⁇ M amino acid day zero) is defined as the minimum expansion of the USEFUL POOL
- the maximum expansion of the USEFUL POOL is calculated from the average of values obtained on the 15th day of fasting, whose value is 344 24 ⁇ 13 45 ⁇ mol / l
- the regression line runs parallel to the x-axis.
- the maximum and minimum expansion POOL can be calculated as described above (see A and B) When there is statistical significance, there are differences in the maximum expansion of the size of the POOL, which is obtained on day zero if the slope decreases or on day 15 when the slope increases At the same time the minimum expansion is reached on day zero if the slope increases and on day 15 when the slope decreases
- the minimum expansion of the USEFUL POOL is calculated from the average of the values obtained on the 15th day of fasting, whose value is 238 09 ⁇ 35 00 ⁇ mol / l
- the USEFUL POOL is considered as the average of the values of the concentrations obtained at zero time plus two standard deviations (table 1)
- USEFUL POOL is what must be achieved after the administration of nutrition With nutrition, the amino acids that increase with fasting return to their normal values and vice versa, those that decrease reach normal values after eating. Finally, the amino acids that do not vary remain significantly unchanged after nutrition
- Table 1 shows the calculation of USEFUL POOL, the average of the concentrations of amino acids extrapolated at zero time plus two standard deviations (2SD) Table 1. Calculation of USEFUL POOL
- the transformation factor is obtained by relating the amount of amino acid (AA) administered ( ⁇ mol / day) and the arterial concentration obtained from amino acids ( ⁇ mol / l) in male Wistar rats 24 months old.
- parenteral nutrition is provided for 24 hours and the arterial concentration of amino acids is determined after that period.
- Factor [AA administered] / [arterial AA]
- Factor [ ⁇ mol / day administered] / [ ⁇ mol / l]
- Table 2 shows the Final Transformation Factor for each amino acid in parenteral nutrition
- the amount of each amino acid ( ⁇ mol / day) that the rat should receive is calculated from the formula in section 1 B (see table 3), that is, based on the Transformation Factor and the size of the USEFUL POOL of each amino acid.
- the Parenteral Nutrition Diet contains the Amino Acid Formula of Table 5 (NPT Diet (%)). Table 5. Amino acid formula in the Parenteral Diet
- Example 2 Amino Acid Formula in Intestinal Nutrition solutions. Use of arterial blood for determination.
- Table 6 shows the Final Transformation Factor for each amino acid in enteral nutrition.
- Table 8 shows the concentrations of amino acids in percentage form obtaining the amino acid formula in% (g of amino acids free / 100 g of total amino acids) in the Enteral Base Diet.
- the Enteral Nutrition Diet contains the Amino acid formula of table 9 (Proposed diet N. enteral (%)), taking into account that the amino acids in this case will be administered as proteins, the proteins being partially hydrolyzed.
- the enteral diet may contain other amino acids (for example L-anserine and L-aspartic acid) depending on the proteins administered.
- Example 3 Amino Acid Formula in Parenteral Nutrition solutions. Use of jejunum for determination.
- jejunum is used instead of arterial blood.
- the jejunum sample is obtained by discarding the first 30 cm from the pylorus and sectioning a 20 cm fragment with a tension of 3.5 g.
- the serosa is separated by longitudinally sectioning the intestine and scraping the luminal face with a slide.
- the material obtained by scraping (muscular and mucous) is deposited in a pre-weighed test tube.
- the tissue sample is weighed, frozen with liquid nitrogen and stored at -80 ° C until processing.
- Tissue treatment is performed according to the method of Adibi S.A .. Am. J. Phvsio 221. 3, 829-838 (1971). An aliquot from the tissue studied is weighed and homogenized with 6% sulfosalicylic acid (1 ml / 100 mg of tissue) at 4 ° C. The supernatant obtained is ultrafiltered for 30 minutes (cut 10000) and frozen until the moment of analysis.
- tissue proteins The measurement of tissue proteins is carried out by the method of Lowry O.H., J. Biol. Chem .. 193. 265-275 (1951).
- Example 4 Amino Acid Formula in Enteral Nutrition solutions. Use of jejunum for determination.
- jejunum is used instead of arterial blood.
- Tissue treatment is performed as described in example 3.
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- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
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Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98921495A EP0917826A1 (en) | 1997-05-28 | 1998-05-26 | Aminoacid formulations for third age persons and process for calculating such formulations |
AU74324/98A AU7432498A (en) | 1997-05-28 | 1998-05-26 | Aminoacid formulations for third age persons and process for calculating such formulations |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ESP9701157 | 1997-05-28 | ||
ES009701157A ES2132021B1 (es) | 1997-05-28 | 1997-05-28 | Formulas de aminoacidos para personas de la tercera edad y procedimiento para el calculo de las mismas. |
Publications (1)
Publication Number | Publication Date |
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WO1998053709A1 true WO1998053709A1 (es) | 1998-12-03 |
Family
ID=8299477
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/ES1998/000149 WO1998053709A1 (es) | 1997-05-28 | 1998-05-26 | Formulas de aminoacidos para personas de la tercera edad y procedimiento para el calculo de las mismas |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0917826A1 (es) |
AU (1) | AU7432498A (es) |
ES (1) | ES2132021B1 (es) |
WO (1) | WO1998053709A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0009056D0 (en) * | 2000-04-12 | 2000-05-31 | Nestle Sa | Composition comprising free amino acids |
AU2001235976A1 (en) * | 2000-12-19 | 2002-07-01 | Malladi Surya Prakasa Sastry | An antidiabetic composition of amino acids |
US20040235923A1 (en) * | 2001-06-08 | 2004-11-25 | Takashi Abe | Body temperature-raising agent of amino acids for eating or drinking and for medical use |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0399656A1 (en) * | 1989-04-24 | 1990-11-28 | Kyowa Hakko Kogyo Kabushiki Kaisha | Nutrient compositions for amino acid supplementation in mammals |
US5221668A (en) * | 1992-02-26 | 1993-06-22 | Abbott Laboratories | Nutritional product for trauma and surgery patients |
WO1993013767A1 (en) * | 1992-01-17 | 1993-07-22 | New England Deaconess Hospital Corporation | Method of preparing total parenteral nutrition solutions |
EP0614616A2 (en) * | 1993-03-09 | 1994-09-14 | Clintec Nutrition Company, An Illinois Partnership | Composition for treating patients with hepatic disease |
FR2710244A1 (fr) * | 1993-07-12 | 1995-03-31 | Urso Michel | Composition diététique à base d'acides aminés essentiels. |
US5504072A (en) * | 1993-10-08 | 1996-04-02 | Sandoz Nutrition Ltd. | Enteral nutritional composition having balanced amino acid profile |
EP0721742A1 (en) * | 1995-01-13 | 1996-07-17 | Clintec Nutrition Company, An Illinois Partnership | Nutrition for elderly patients |
-
1997
- 1997-05-28 ES ES009701157A patent/ES2132021B1/es not_active Expired - Lifetime
-
1998
- 1998-05-26 AU AU74324/98A patent/AU7432498A/en not_active Abandoned
- 1998-05-26 EP EP98921495A patent/EP0917826A1/en not_active Withdrawn
- 1998-05-26 WO PCT/ES1998/000149 patent/WO1998053709A1/es not_active Application Discontinuation
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0399656A1 (en) * | 1989-04-24 | 1990-11-28 | Kyowa Hakko Kogyo Kabushiki Kaisha | Nutrient compositions for amino acid supplementation in mammals |
WO1993013767A1 (en) * | 1992-01-17 | 1993-07-22 | New England Deaconess Hospital Corporation | Method of preparing total parenteral nutrition solutions |
US5221668A (en) * | 1992-02-26 | 1993-06-22 | Abbott Laboratories | Nutritional product for trauma and surgery patients |
EP0614616A2 (en) * | 1993-03-09 | 1994-09-14 | Clintec Nutrition Company, An Illinois Partnership | Composition for treating patients with hepatic disease |
FR2710244A1 (fr) * | 1993-07-12 | 1995-03-31 | Urso Michel | Composition diététique à base d'acides aminés essentiels. |
US5504072A (en) * | 1993-10-08 | 1996-04-02 | Sandoz Nutrition Ltd. | Enteral nutritional composition having balanced amino acid profile |
US5504072B1 (en) * | 1993-10-08 | 1997-08-26 | Sandoz Nutrition Ltd | Enteral nutritional composition having balanced amino acid profile |
EP0721742A1 (en) * | 1995-01-13 | 1996-07-17 | Clintec Nutrition Company, An Illinois Partnership | Nutrition for elderly patients |
Also Published As
Publication number | Publication date |
---|---|
ES2132021B1 (es) | 2000-02-16 |
AU7432498A (en) | 1998-12-30 |
ES2132021A1 (es) | 1999-08-01 |
EP0917826A1 (en) | 1999-05-26 |
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