WO1998049286A3 - Evolution orientee d'enzymes et d'anticorps - Google Patents

Evolution orientee d'enzymes et d'anticorps Download PDF

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Publication number
WO1998049286A3
WO1998049286A3 PCT/US1998/008714 US9808714W WO9849286A3 WO 1998049286 A3 WO1998049286 A3 WO 1998049286A3 US 9808714 W US9808714 W US 9808714W WO 9849286 A3 WO9849286 A3 WO 9849286A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
antibodies
enzymes
directed evolution
selecting
Prior art date
Application number
PCT/US1998/008714
Other languages
English (en)
Other versions
WO1998049286A9 (fr
WO1998049286A2 (fr
Inventor
Brent Iverson
George Georgiou
Gang Chen
Mark J Olsen
Patrick S Daugherty
Original Assignee
Univ Texas
Brent Iverson
George Georgiou
Gang Chen
Mark J Olsen
Patrick S Daugherty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas, Brent Iverson, George Georgiou, Gang Chen, Mark J Olsen, Patrick S Daugherty filed Critical Univ Texas
Priority to AU71710/98A priority Critical patent/AU7171098A/en
Publication of WO1998049286A2 publication Critical patent/WO1998049286A2/fr
Publication of WO1998049286A3 publication Critical patent/WO1998049286A3/fr
Publication of WO1998049286A9 publication Critical patent/WO1998049286A9/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés de sélection de protéines, dans de grandes bibliothèques présentant des caractéristiques désirables. Des procédés d'expression d'enzymes et d'anticorps sur la surface de cellules hôtes et de sélection d'activités désirées sont donnés à titre d'exemple. Ces procédés comparés aux procédés actuels présentent l'avantage d'une utilisation rapide et facile et sont une source de quantités importantes de la protéine considérée, sans clonage supplémentaire.
PCT/US1998/008714 1997-05-01 1998-04-30 Evolution orientee d'enzymes et d'anticorps WO1998049286A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU71710/98A AU7171098A (en) 1997-05-01 1998-04-30 Directed evolution of enzymes and antibodies

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84706397A 1997-05-01 1997-05-01
US08/847,063 1997-05-01

Publications (3)

Publication Number Publication Date
WO1998049286A2 WO1998049286A2 (fr) 1998-11-05
WO1998049286A3 true WO1998049286A3 (fr) 1999-03-25
WO1998049286A9 WO1998049286A9 (fr) 1999-04-29

Family

ID=25299655

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/008714 WO1998049286A2 (fr) 1997-05-01 1998-04-30 Evolution orientee d'enzymes et d'anticorps

Country Status (2)

Country Link
AU (1) AU7171098A (fr)
WO (1) WO1998049286A2 (fr)

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US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
AU9395398A (en) * 1997-09-19 1999-04-12 Chiron Corporation Subtractive protein screening for gene identification
KR100313135B1 (ko) 1999-02-22 2001-11-05 윤덕용 세포 표면 발현 모체로서의 대장균 유래 세포외막 단백질 ｃ(ｏ
WO2000052193A1 (fr) * 1999-03-04 2000-09-08 Suntory Limited Procede de controle de clivage par la protease de l'ompt
KR100312070B1 (ko) * 1999-03-15 2001-11-03 박호군 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 제조방법
AU7646100A (en) * 1999-11-05 2001-05-14 Novozymes A/S A high throughput screening (hts) method
EP1297142B1 (fr) 2000-06-29 2009-01-14 Abbott Laboratories Anticorps a double specificite, procedes de fabrication et d'utilisation
US7083945B1 (en) 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US7094571B2 (en) * 2000-10-27 2006-08-22 The Board Of Regents Of The University Of Texas System Combinatorial protein library screening by periplasmic expression
US7611866B2 (en) 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
DE10303974A1 (de) 2003-01-31 2004-08-05 Abbott Gmbh & Co. Kg Amyloid-β(1-42)-Oligomere, Verfahren zu deren Herstellung und deren Verwendung
JP2009510002A (ja) 2005-09-30 2009-03-12 アボット ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 反発誘導分子（ｒｇｍ）タンパク質ファミリーのタンパク質の結合ドメイン、及びその機能的断片、及びそれらの使用
DK1976877T4 (en) 2005-11-30 2017-01-16 Abbvie Inc Monoclonal antibodies to amyloid beta protein and uses thereof
PT1954718E (pt) 2005-11-30 2014-12-16 Abbvie Inc Anticorpos anti-globulómeros aβ, suas porções de ligação ao antigénio, correspondentes hibridomas, ácidos nucleicos, vectores, células hospedeiras, métodos de produção dos ditos anticorpos, composições compreendendo os ditos anticorpos, usos dos ditos anticorpos e métodos para uso dos ditos anticorpos
JP5259423B2 (ja) 2006-02-01 2013-08-07 セファロン・オーストラリア・ピーティーワイ・リミテッド ドメイン抗体構築物
US8455626B2 (en) 2006-11-30 2013-06-04 Abbott Laboratories Aβ conformer selective anti-aβ globulomer monoclonal antibodies
US8324350B2 (en) 2006-12-29 2012-12-04 Abbott Laboratories Dual-specific IL-1α/IL-1β antibodies
WO2008104386A2 (fr) 2007-02-27 2008-09-04 Abbott Gmbh & Co. Kg Méthode de traitement d'amyloïdoses
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
MX2011007049A (es) * 2009-02-24 2011-08-03 Esbatech Alcon Biomed Res Unit Metodos para identificar inmunoligadores de los antegenos de la superficie celular.
SG176922A1 (en) 2009-06-19 2012-01-30 Medimmune Llc Protease variants
JP5951498B2 (ja) 2009-12-08 2016-07-13 アッヴィ・ドイチュラント・ゲー・エム・ベー・ハー・ウント・コー・カー・ゲー 網膜神経線維層変性の治療に使用するためのｒｇｍａタンパク質に対するモノクローナル抗体
MX360403B (es) 2010-04-15 2018-10-31 Abbvie Inc Proteinas de union a amiloide beta.
EP2603524A1 (fr) 2010-08-14 2013-06-19 AbbVie Inc. Protéines de liaison bêta-amyloïdes
JP2014204669A (ja) * 2011-08-18 2014-10-30 公立大学法人名古屋市立大学 抗体とその利用
NZ625403A (en) 2012-01-27 2016-03-31 Abbvie Inc Composition and method for diagnosis and treatment of diseases associated with neurite degeneration
CN111999235A (zh) * 2020-07-03 2020-11-27 青岛农业大学 一种利用流式细胞术快速检测有活力的食用菌原生质体数量的方法

Citations (2)

* Cited by examiner, † Cited by third party
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EP0095361A1 (fr) * 1982-05-25 1983-11-30 Eli Lilly And Company Vecteurs de clonage pour l'expression de protéine exogène
WO1994026787A1 (fr) * 1993-05-07 1994-11-24 The Board Of Trustees Of The Leland Stanford Junior University Procede pour generer des bibliotheques d'anticorps de phages specifiques de types cellulaires

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0095361A1 (fr) * 1982-05-25 1983-11-30 Eli Lilly And Company Vecteurs de clonage pour l'expression de protéine exogène
WO1994026787A1 (fr) * 1993-05-07 1994-11-24 The Board Of Trustees Of The Leland Stanford Junior University Procede pour generer des bibliotheques d'anticorps de phages specifiques de types cellulaires

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
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LITTLE M ET AL: "Human antibody libraries in Escherichia coli", JOURNAL OF BIOTECHNOLOGY, vol. 41, no. 2, 31 July 1995 (1995-07-31), pages 187-195, XP004036935 *

Also Published As

Publication number Publication date
WO1998049286A9 (fr) 1999-04-29
AU7171098A (en) 1998-11-24
WO1998049286A2 (fr) 1998-11-05

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