WO1998039476A1 - Methode d'identification d'un gene codant une proteine membranaire de type ii - Google Patents

Methode d'identification d'un gene codant une proteine membranaire de type ii Download PDF

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Publication number
WO1998039476A1
WO1998039476A1 PCT/JP1998/000830 JP9800830W WO9839476A1 WO 1998039476 A1 WO1998039476 A1 WO 1998039476A1 JP 9800830 W JP9800830 W JP 9800830W WO 9839476 A1 WO9839476 A1 WO 9839476A1
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WO
WIPO (PCT)
Prior art keywords
protein
gene encoding
membrane
membrane protein
type
Prior art date
Application number
PCT/JP1998/000830
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English (en)
Japanese (ja)
Inventor
Seishi Kato
Midori Kobayashi
Original Assignee
Sagami Chemical Research Center
Protegene Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sagami Chemical Research Center, Protegene Inc. filed Critical Sagami Chemical Research Center
Publication of WO1998039476A1 publication Critical patent/WO1998039476A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Definitions

  • FIG. 2 shows the hydrophobicity / hydrophilicity profile of the protein encoded by HP10085 determined by the Kyte-Doolittle method.
  • FIG. 4 shows the results of the fibrin plate assay.
  • a culture supernatant is prepared. Whether or not the reporter protein remains on the membrane surface is examined using the reporter protein detection method used. In the case of enzymes, use the corresponding enzyme activity detection method.
  • a fibrin sheet was placed so as to be in close contact with the surface of the cells, and the cells were incubated at 37 ° C overnight. As a result, lysis spots were observed on the cell surface expressing the pSSD cells HP10085 and pKAl-UPA (FIG. 4B).
  • the N-terminal hydrophobic region of HP10085 functions not as a secretory signal sequence but as a transmembrane domain, and the N-terminal side is in the cytoplasm and the C-terminal side is extracellular. It has been experimentally proved to be a transmembrane domain of membrane proteins.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On décrit une méthode qui permet d'identifier un gène codant une protéine membranaire de type II. Pour ce faire, on transfère dans une cellule animale un gène hybride d'un fragment d'ADN codant la protéine à identifier et un gène codant une protéine marqueur. On exprime ensuite ledit gène hybride dans ladite cellule animale, puis on identifie le gène codant la protéine membranaire de type II en utilisant comme indice l'expression de la protéine marqueur sur la surface de la membrane.
PCT/JP1998/000830 1997-03-03 1998-02-27 Methode d'identification d'un gene codant une proteine membranaire de type ii WO1998039476A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP9/47775 1997-03-03
JP9047775A JPH10243784A (ja) 1997-03-03 1997-03-03 Ｉｉ型膜蛋白質をコードする遺伝子の同定方法

Publications (1)

Publication Number Publication Date
WO1998039476A1 true WO1998039476A1 (fr) 1998-09-11

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PCT/JP1998/000830 WO1998039476A1 (fr) 1997-03-03 1998-02-27 Methode d'identification d'un gene codant une proteine membranaire de type ii

Country Status (2)

Country Link
JP (1) JPH10243784A (fr)
WO (1) WO1998039476A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032894A1 (fr) * 1999-11-04 2001-05-10 Zymogenetics, Inc. Presentation de surface cellulaire de proteines par des cellules hotes recombinantes
US6686168B1 (en) * 1999-11-04 2004-02-03 Zymogenetics, Inc. Cell surface display of proteins by recombinant host cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AOKI D., ET AL.: "GOLGI RETENTION OF A TRANS-GOLGI MEMBRANE PROTEIN, GALACTOSYL- TRANSFERASE, REQUIRES CYSTEINE AND HISTIDINE RESIDUES WITHIN THE MEMBRANE-ANCHORING DOMAIN.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 89., 1 January 1992 (1992-01-01), US, pages 4319 - 4323., XP002911161, ISSN: 0027-8424, DOI: 10.1073/pnas.89.10.4319 *
HAMANN J., ET AL.: "AICL: A NEW ACTIVATION-INDUCED ANTIGEN ENCODED BY THE HUMAN NK GENE COMPLEX.", IMMUNOGENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 45., 1 January 1997 (1997-01-01), DE, pages 295 - 300., XP002911166, ISSN: 0093-7711, DOI: 10.1007/s002510050208 *
JACOBS K. A., ET AL.: "A GENETIC SELECTION FOR ISOLATING CDNAS ENCODING SECRETED PROTEINS.", GENE., ELSEVIER, AMSTERDAM., NL, vol. 198., 1 January 1997 (1997-01-01), NL, pages 289 - 296., XP002911165, ISSN: 0378-1119, DOI: 10.1016/S0378-1119(97)00330-2 *
KLEIN R. D., ET AL.: "SELECTION FOR GENES ENCODING SECRETED PROTEINS AND RECEPTORS.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 93., 1 January 1996 (1996-01-01), US, pages 7108 - 7113., XP002911164, ISSN: 0027-8424, DOI: 10.1073/pnas.93.14.7108 *
TANG B. L., ET AL.: "THE TRANSMEMBRANE DOMAIN OF N-GLUCOSAMINYLTRANSFERASE I CONTAINS A GOLGI RETENTION SIGNAL.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 267., no. 14., 1 January 1992 (1992-01-01), US, pages 10122 - 10126., XP002911162, ISSN: 0021-9258 *
TASHIRO K., ET AL.: "SIGNAL SEQUENCE TRAP: A CLONING STRATEGY FOR SECRETED PROTEINS AND TYPE I MEMBRANE PROTEINS.", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 261., 1 January 1993 (1993-01-01), US, pages 600 - 603., XP002911163, ISSN: 0036-8075, DOI: 10.1126/science.8342023 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032894A1 (fr) * 1999-11-04 2001-05-10 Zymogenetics, Inc. Presentation de surface cellulaire de proteines par des cellules hotes recombinantes
US6686168B1 (en) * 1999-11-04 2004-02-03 Zymogenetics, Inc. Cell surface display of proteins by recombinant host cells
US7125973B2 (en) * 1999-11-04 2006-10-24 Zymogenetics, Inc. Cell surface display of proteins by recombinant host cells

Also Published As

Publication number Publication date
JPH10243784A (ja) 1998-09-14

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