WO1998011228A2 - Genetic control of fruit ripening - Google Patents

Genetic control of fruit ripening Download PDF

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Publication number
WO1998011228A2
WO1998011228A2 PCT/GB1997/002424 GB9702424W WO9811228A2 WO 1998011228 A2 WO1998011228 A2 WO 1998011228A2 GB 9702424 W GB9702424 W GB 9702424W WO 9811228 A2 WO9811228 A2 WO 9811228A2
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WIPO (PCT)
Prior art keywords
seq
plants
sequence
ripening
plant
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PCT/GB1997/002424
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French (fr)
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WO1998011228A3 (en
Inventor
Graham Barron Seymour
Colin Roger Bird
Rosybel de Jesús MEDINA-SUAREZ
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Zeneca Limited
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Priority claimed from GBGB9618862.8A external-priority patent/GB9618862D0/en
Priority claimed from GBGB9708366.1A external-priority patent/GB9708366D0/en
Application filed by Zeneca Limited filed Critical Zeneca Limited
Priority to JP09533834A priority Critical patent/JP2000517164A/en
Priority to AU41291/97A priority patent/AU4129197A/en
Priority to EP97939069A priority patent/EP0931150A2/en
Priority to CA002262505A priority patent/CA2262505A1/en
Publication of WO1998011228A2 publication Critical patent/WO1998011228A2/en
Publication of WO1998011228A3 publication Critical patent/WO1998011228A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life

Definitions

  • This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression. More specifically it relates to the modulation of the ripening and/or tissue senescence characteristics and plants derived therefrom.
  • One suitable application of the present invention is the modulation of ripening and/or senescence processes in plants of the genus Musa (referred to herein as banana).
  • Overexpression may be achieved by insertion of one or more than one extra copies of the selected gene. It is, however, not unknown for plants or their progeny. originally transformed with one or more than one extra copy of a nucleotide sequence, to exhibit the effects of underexpression as well as overexpression.
  • Gene control by any of these methods requires the insertion of a selected gene or genes into plant material which can be regenerated into plants.
  • This transformation process can be performed via a number of methods, for example: the Agrobacterium-m ⁇ diatsd transformation method.
  • microparticles of dense material are fired at high velocity at the target cells where they penetrate the cells, opening an aperture in the cell wall through which DNA may enter.
  • the DNA may be coated on to the microparticles or may be added to the culture medium.
  • microinjection. the DNA is inserted by injection into individual cells via an ultrafine hollow needle.
  • Another method viz. fibre-mediated transformation, applicable to both monocots and dicots, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride "whiskers", .and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell.
  • Bananas are a globally important fruit crop. They are not only a popular dessert fruit, but represent a vital carbohydrate staple in the tropics with as many as 100 million people subsisting on bananas and plantains as their main energy source.
  • the cultivated dessert banana is commonly triploid, parthenocarpic and belongs to the Musa AAA genome group, eg. Cavendish subtypes.
  • Bananas are climacteric fruits and ripening is regulated by ethylene produced by the fruit and involves numerous biochemical changes including the conversion of starch to sugars, cell wall disassembly, synthesis of volatile compounds, changes in phenolic constituents and degradation of chlorophyll in the peel.
  • a method of modulating the ripening or tissue senescence process in pl.ants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID-Nos. 1 -57. regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
  • the said polynucleotide may be obtained from the cDNA library having the NCIMB Accession Number 40814.
  • a method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
  • the invention further provides a method as described above wherein the said polynucleotide is capable of modulating the production of pectate lyase and more specifically the polynucleotide comprises at least one of the sequences depicted in the sequence listings as SEQ-ID-Nos. 13-18.
  • a preferred method for inserting the said polynucleotides into plant material according to the method of the present invention may be selected from the group comprising the Agrobacte ⁇ um, microparticle bombardment, fibre mediated or direct insertion methods.
  • the invention further provides plants, their progeny and seed and material obtained from said plants, produced according to the method of the present invention. It is preferred that the said plants, their progeny and seed and material obtained from said plants are derived from plants of the genus Musa.
  • the present invention also provides a vector functional in plants comprising a promoter region which is operable in plant cells, at least one of the polynucleotide sequences described above and a transcription termination sequence.
  • the promoter may be constitutive, developmentally regulated or switchable.
  • the promoter may also be tissue specific or organ specific.
  • a banana produced via the preceding method having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described above.
  • the present invention also provides an improved method of controlling plant pathogens comp ⁇ sing the application of an anti-pathogenic agent to plants, characterised in that plants to which the said agent is applied, are plants according to the present invention.
  • the gene sequences of the present invention may be synthesised ab initio, using the sequence data in the sequence listing provided herewith, or isolated from a library using the standard techniques know within the art.
  • sequences depicted in the sequence listing or parts thereof may also be used to create oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins.
  • oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins.
  • To assist the isolation of these polynucleotides we have deposited with the National Collection of Industrial & Marine Bacte ⁇ a, St. Machar Drive, Aberdeen, UK, a cDNA library of the banana peel ripening related genes. The library was deposited on 9th July 1996 and has the NCIMB Accession Number 40814.
  • this invention is based on the identification of genes which encode proteins involved in banana ripening-related processes, specifically within banana pulp.
  • DNA sequences according to the sequence listing or those sequences obtainable from the deposited library may be used in the process of modifying the plant ripening characte ⁇ stics of plants and/or fruit.
  • banana plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characte ⁇ stics: improved resistance to damage du ⁇ ng harvest, packaging and transportation due to slowing of the ⁇ pening and over- ⁇ penmg processes; longer shelf life and better storage characte ⁇ stics due to reduced activity of degradative pathways (e.g.
  • improved processing characte ⁇ stics due to changed activity of proteins/enzymes contributing to factors such as: viscosity, solids, pH, elasticity; improved flavour and aroma at the point of sale due to modification of the sugar/acid balance and other flavour and aroma components responsible for characteristics of the ⁇ pe fruit; modified colour due to changes m activity of enzymes involved in the pathways of pigment biosynthesis (e.g. lycopene, ⁇ -carotene. chalcones and anthocyamns), increased resistance to post-harvest pathogens such as fungi.
  • the activity of the ⁇ pemng-related proteins may be either increased or reduced depending on the characte ⁇ stics desired for the modified plant part (fruit, leaf, flower, etc).
  • the levels of protein may be increased: for example, by incorporation of additional genes.
  • the additional genes may be designed to give either the same or different spatial and temporal patterns of expression m the fruit "Antisense” or "partial sense” or other techniques may be used to reduce the expression of ripening-related protein.
  • the activity of each ripening-related protein or enzyme may be modified either individually or in combination with modification of the activity of one or more other ripening- related proteins/enzymes.
  • the activities of the ripening-related proteins/enzymes may be modified in combination with modification of the activity of other enzymes involved in fruit ripening or related processes.
  • DNA constructs according to the invention for gene silencing may comp ⁇ se a base sequence at least 10 bases (preferably at least 35 bases) in length for transc ⁇ ption into RNA. There is no theoretical upper limit to the base sequence, it may be as long as the relevant mRNA produced by the cell but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. When using genomic DNA as the source of a base sequence for transc ⁇ ption it is possible to use either intron or exon regions or a combination of both.
  • a suitable cDNA or genomic DNA or synthetic polynucleotide may be used as a source of the DNA base sequence for transcription.
  • the isolation of suitable npening-related sequences is desc ⁇ bed above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCIMB in Aberdeen. Sequences coding for the whole. or substantially the whole, of the approp ⁇ ate npening-related protein may thus be obtained Suitable lengths of this DNA sequence may be cut out for use by means of rest ⁇ ction enzymes.
  • Constructs suitable for expression of the approp ⁇ ate ripening-related sequence in banana cells may be produced using a cDNA sequence selected from the deposited library having the NCIMB Accession Number 40814 or the gene sequence as found in the chromosome of the banana plant. Recombinant DNA constructs may be made using standard techniques.
  • modulation means an increase or decrease of the desired effect More specifically ''modulation of the npening or tissue senescence process in plants of the genus Musa " means an increase or decrease in production of a ⁇ pemng related protein resulting from the method as desc ⁇ bed above For example, where an increased ⁇ pemng related protein is desired, plants may be transformed according to the method as desc ⁇ bed above and those plants exhibiting the desired effect may be selected from the population of trans formants. Furthermore, it may be desirable to provide a plant with modulated ripening or tissue senescence characteristics by increasing the production of one protein and decreasing the production of another protein in the same plant.
  • a banana fruit, modified using the present method, and having decreased levels of the enzyme pectate lyase would be beneficial because pulp softening would require a substantially longer time when compared with a control.
  • another ripening related protein such as an antifungal protein in the same fruit using the present method, would complement the extended life of the banana pulp with increased resistance to disease.
  • Increase in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing a protein and thereby increasing protein levels when compared with a control plant.
  • Decrease in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing an mR A which is capable of interfering with endogenous mRNA to such a degree that the levels of translated protein are reduced when compared with a control plant.
  • Ripening process of plants means the process of maturing or developing .
  • Senescence means the progressive deterioration in function of cells, tissues, organs etc.. related to the period of time since that function commenced..
  • Control plant means a comparable plant used for the purposes of determining modulation of the ⁇ pening or tissue senescence process effect in plants.
  • the control pl ⁇ int is usually of the same species and variety as the material used before the transformation process and is grown in the same conditions, (usually with the transformant selection step modified in some way on the part of the control plant), as the transformed plants.
  • the control plant may comprise an untransformed control plant or a transformed control plant providing it has not already been transformed with the same polynucleotide sequence as the plant material to be transformed.
  • Plant material includes plant ceils and any other type of plant regenerable material. The following examples further illustrate the invention but are not to be construed to limit the scope thereof:
  • FIGURE 1 Plant transformation vector pUN. containing the UBI polyubiquitin promoter.
  • FIGURE 2 Plant transformation vector pSHYN, containing hygromycin resistance gene for selection of transformed plants.
  • FIGURE 3 Plant transformation vector pAN. containing the banana ACC oxidase promoter.
  • the first and second strands of the cDNAs were svnthesised from the messenger RNAs using a commercial cDNA synthesis kit (Catalog No. 200450, ZAP ExpressTM Gold Cloning kit. Stratagene Ltd. Cambridge. Ca bs, UK). Double stranded cDNAs were cloned into the ZAP ExpressTM vector, packaged, mixed with plating bacteria to determine titre and for library screening, following instructions of the suppliers protocol. 1.3 Screening of the cDNA library from banana pulp.
  • the unamplified cDNA library from ripening banana pulp was differentially screened using cDNA from unripe and ripening banana peel tissue.
  • a proportion of the library was plated individually at low density and duplicate plaque lifts made onto Hybond N nylon filters (Amersham) according to t e manufacturer's instructions.
  • One filter was hybridised to dCTP radiolabelled cDNA from green fruit and the duplicate filter hybridised to dCTP radiolabelled cDNA from ripening fruit. Hybridization's were at high stringency. Plaques hybridising preferentially with ripening or green radiolabelled cDNA were picked and re- plated for a second round of selection by differential screening. These clones were numbered as ripening up- or down-regulated peel clones. The clones were in-vivo excised from the ZAP expressTM vector into the pBK-CMV phagemid vector using the E.xAssistTM interference-resistant helper phage, following instructions from manufacturers protocol.
  • the ripening cDNA library from pulp tissue were prepared with an efficiency of 3.2 x 10 5 plaque-forming units per microgram of cDNA.
  • the sizes of the inserts in the peel library was 0.4 - 6.7 Kb with a mean size insert of 1.47 Kb.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is svnthesised by polymerase chain reaction using synthetic p ⁇ mers incorporating BamHI rest ⁇ ction sites suitable for clomng between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology,
  • the truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3'end termination sequences in the vector pAN.
  • the truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants.
  • Transformed Musa plants containing the vectors are produced by the method described in Sagi et al. (1995) Biotechnology. Vol. 13 pp 481-485. Regenerated transformed plants are identified by their ability to grow on hygromycin and grown to maturity. Ripening fruit are analysed for a modulation in their ripening related or senescence characteristics. Other suitable transformation methods for banana are described in Sagi et al. ( 1994) Plant Cell Reports. Vol. 13. pp 262-266. and May et al. (1995) Biotechnology. Vol. 13 pp 486- 492.
  • GCGAGAAGAG CAAAATCCTG ATCATCGGGG GCACCGGGTA CATCGGCAAG TTCATCGTGT 120
  • NTTTCCTCCC GGTATTANGA CAGGCAGGAG CCACTGGTCC TCCCACGGAC AAGGTTGTCA 600
  • TCTTANGTGA CGGGAACACA AAAGCGATCT TTCTCAATGA ANACACATCC GGACNTNCAC 660
  • TANGACAGGC AGGACCACTG GTCCTCCCCA CNGACAAGGT TGTCNTCTTA GGTGACNGGA 600
  • CAAGCCCTCA GGAACTCCAA CATCCAAGTC CTGTTGGATG TCCCCCGATC CGACGTGCAG 300
  • CCTGCAACAT CTTCTCCACC CAGGACCACG CCGCTGCCGC CATTGCCCGC GACTCCGCCG 360 CCGTCTTCGC CTGGAAGGGA GAGACCCTCG CCGAGTACTG GTGGTGCACC GAGCGATGCC 420 TCGACTGGGG CCCCAACGGT GGCCCCGACC TCATCGTCGA CAAAGGGGGT GACGCCACTC 480
  • CTCCAGGTCN AACCCCNAAA ATTACCGCCC AGATNAAAGA ACCCTCCTCC GCTTTTTGAA 660
  • GAAAACANCN CTGGCGTTCA NCGAATCTAC CANATGCCAG GGCCANCNGG GGCCCCTTGC 720
  • CTCCGGACTT CGGCAACTTT TTCAAGGACC ATGGCGGCTC CTGGGANACA CCCTACGGGC 540
  • ANCATGATTC ATCCCGGGCN TGGACTTCTC NGAAGGGGAA CANTCCTCAG GGTGTTCCGG 840
  • NTTANATTNC ACCGTCCTCC AATTNTCCCC ANTTTCTTTN GCCGCNGGNN NCGGGCCCCN 720
  • TGGTCATCCA GCTGAAGGAA GGAGCTCATC ATGAAACTCC CACANAGACC ATCGACGGCC 480 GGGGCGCCAG CGTCCACATC TCCGGGGGGC CGTGCATCAC CACCAGTNCG TCNCCAACAT 540
  • GCCTCCACAT CCACGACTGC AAGCCCACCG
  • TCTCCTTCTC 120 ACTATGGATG GAGAACCATG GCTGATGGGG ATGCCGTTTC CATTTTCGGC TCCAGCCACA 180
  • AATCNCC 727 (2) INFORMATION FOR SEQ ID NO: 17:
  • CCGGCAGCGG CTCGCTGACT GCGCCATCGG GTTCGGGAAG AACGCGATTT GGGGCAGGGA 300 CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACGAC CCCGTCAATC CGAAAACGGG 360
  • CTTCCNTCCC AACCCCCATT TCCTTGGGAT TNCCCCCCGC CAATTCCCCC ATTTTCCAAC 780
  • NAACTTNCTT TGAACCCNCC CNTGAANAAG GTCCTTGCTG CTGGGTCCCA CCAACAATTT 840
  • NA 842
  • TGGGGAGCCT CACGTGGTGG TACAACGAGG AGGAGGACTC CGACTTCTCC ATCGCCTTCT 360 TAGGCGATAC CGCGACAGCT GTGCGACCGG GCGACATCGC CTACTTCTTC TTGGCAGGAT 420
  • TGANCGCGTA NCGGCATATA TCAATGTGAA AAGTGGTGGC TGTTCTGCCT CGGTGACCCT 660 TNATNAACTG GCNGCNCTGG GAAGATCAGG TTCTCCGTCN ACCTCCAAAA ATCCANCCTA 720
  • TCCACTCTCN CTGGGANAAT TTTGAAAAAT GTTNAGCTTC TAAATGATCT CCNGGACCNA 720
  • TGGTGGTCGG TTGCAGGGCG AAGGGATGGG GATGGGGAGG CCGAGCGGAG CTTGGTCGAC 120 CCCGTGGTTG GTGGTGGTTC TTGTGCATTG GCTCCTCTGG GCCACCGAGA GGAGGCGAGG 180
  • CCANCANAAA CAACTGCANA ACNTTNCNTN CCCAAGGATC TGTCCAACCT TACTCCCCNA 600
  • CNCNAACCCN AATACGGNTT GGCGCNGTCC CNGCACCCNC TGGAAACCCC TNGTNGGGAA 720 CNANTTGAAN CNGAAGGGGA AAGGCNTTCT TTNCCGAACA GGGAT 765
  • NANATCNCCC NCTGNTGATC TGGTGGNAAT CCCCCCCTTG TCCTGTGATT CTTGCTCTTG 360 ACNTTTTTCT CCCNTGTCNN ANCTCTCTNC CTNCTCTGGG TNTTGGGTCT TCCCCTGTTG 420
  • NCAGGGGAAG ACCATTACCT TGGAGGTGGA NANCTCNGAC ACGATTGATA ATGTCNAGGC 480 AAAGATCCAN GACAAGGANG GGATTCCACC GGATCAGCAN ANGCTGATCT TTGCTGGGAA 540 ACACTGGANG AAGGGCGCAC CCTGGCAGAT TNCNACATTC CNAAAGAATC CACCTTCACC 600
  • AAAGGAAATN AANCCNNTTG GGTTTTCCCC TTTGGCCTTT TTTTTGTTTG AAAATCCTNT 780
  • GGCACNANCC CGCTTCGGTA GCCATTATTG GTGCCCGTAG AGCGAGTGCT GAGATGGCTT 60
  • CCGTACCTCC TGCTCACAAG CCCCGAACTC CGCCGCCGGT TTACAAGTCT CCACCACCGA ⁇ 0 NCCACCGTCC TTACCCTCCA TCGACGCCAC CCCACCATCC GAAGCACCCG CCTTCTCAGC 540
  • GGGGTTTTTN TTAANATTTN
  • AANAAANCNA ANTNTTTTCC CCCCCNTNTT GGTTNATGGC 780 CTATGGGTTT GTTTCTCTTC TTGGCTTTTC CCGG 814
  • TCNTACCCAA CCCACCTAAG CTGCCTCCCG GTGGCCCGCC CGTGTTTCCC CTCCTTCAAN 540
  • TCCAGTGCCC TTGAGCTCCC CCTGCGGATC ACATCNTCGA TCCTCTTTGC CCGTGGTGAA 540 TTTTMTTGAG CATTCCTTTG TGTCTTTACT TCCCTTTGTT TTTGGCTTTT TGATGACGAA 600
  • CTATTCCGAC TCATCGAAAG TCGGCTATGT TCTTGAAGGA AGAGCGGTGG TGGGGCTAAC 240 ACTCTATGGA GAGACCGAGC AGAGGATACT GCTGCTTGAG AAGGGAGATG TGGTAGCGGT 300
  • TTCTCATCNA NATANCCGTA ATTAANGACT TCCGGTCATG CNANAATNCC CNACCNANGA 720
  • GTAACACCGT CAAGTACGAT TTCCCATCCT TCGGGTTGTG GCGTTCTGCT ACTTGGCATT 480
  • AACTCCCCCA TCTCCTGGGG CGTTGGCGAT GGACTCCGGC GAATGGTCCT CCTACCTCCC 540
  • CAAGTNACTA ACAGCAGTGG CTACTTGGTG CTTCNTTGGG GTGCAATTCA TAATANAAGG 540
  • TCTCANGANA CCTTTTAAAA AATCTGGTTC CCCATCNTCC GTNAAAATGG AGATTGATGA 660 TCCTGAAATA CCAGCAGTNG AATTTCTCCT TANTTTTGNA ACAAATNCTC AAAAAAACCN 720
  • GGCTCATGCG CTGGCTATGG GGGTGTCGTC GACTCGGAGC CAAGGACCTT CCTAAACAGA 180 AGGGTTCTAC ACTTGAGGAA CCAAACCACC GCTTACGAAG GATTGAGATC TCGAAATGTG 240
  • CTNTCCCCGG AACTGATTTT NAAAAACANC CCCTAAANTT TANCCTTTTN TNCCCNGCCN 720
  • NCCNAATACT TTTCCTGGAA CCATTTNGGG GANAANNTTG TTNTTTGTTG CNAATTAATT 780 GGCNCCCTGG TCCTCCTNCC TGCTACTTAA AAATTTTNTT TCNTNT 826
  • TTTTGTNCCN GCNNCTTTGG AACCNCCCNG GATCTNGATN TTANCACCCC CAANTCTTAT 780 CCTGGAACTT TTGGGGNAAA NTTTGTNTTT GTT 813
  • AAGAATTGAC AGGGTTTTCA TTGATCATCC TTTGTTTCTT GAAAAAGTGT GGGGAAAAAC 600 TGGGAAGAAT GATATNTGGT CCTGTCCCNG GAACTGATTA TAAAACACCA GCTAANATTA 660
  • TTTGTACATC ATCTTTATCG ACAACATCAC AATCAGGATT GAAGGGACCC ATATGAAACC 360
  • CTGTGATGCC TTCTATGACA GTGTCAGCAA GTCCACCAGT TGTTGCGCAC ATGGGGGGAA 420 TTCCATATCG CATGCCCTGA AAGCTGGATA AGGCCACAAG GTTCGAATCT GCTGGTAAGA 480
  • TCCCCTCAAA AGAACACCCG TGCGGCCCAA AATCCGGNCC CCCGGTGGTT CCCCCTTTCN 720 CTTTAANGGT CCCCAATGCC CCTGNTTTAA CTGNCTCCCC CCCANGCNCG GCCCCGGCCC 780
  • CTTAGCAGAT GCTTGTGCCA CAGGCAACTA CGAATACGTG AACATCGCCA CCCTTTTCAA 240
  • GATAGCCTCC TCATTCACTA TGGCGATCCG ATCGCCAGCT TCGCTGCTGT TATTTGCGTT 120
  • CTCCACAAAC CTTCNTGCAT TCTCCCCAAA CCTATCAATC TTGCNAAATG CGTTCAACAA 780 TTNGGGTCTT TTCATCCCCG CCCAAAACT 809

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Abstract

A method of modulating the ripening and/or senescence characteristics in plants of the genus Musa comprises transforming plants with one or more sequences obtainable from the deposited cDNA library having the accession number 40184, regenerating said plants and selecting from the population of transformants those plants having modulated and/or tissue senescence characteristics.

Description

GENETIC CONTROL OF FRUIT RIPENING
This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression. More specifically it relates to the modulation of the ripening and/or tissue senescence characteristics and plants derived therefrom. One suitable application of the present invention is the modulation of ripening and/or senescence processes in plants of the genus Musa (referred to herein as banana).
Two principal methods for the control of expression are known, viz.: overexpression and underexpression. Overexpression may be achieved by insertion of one or more than one extra copies of the selected gene. It is, however, not unknown for plants or their progeny. originally transformed with one or more than one extra copy of a nucleotide sequence, to exhibit the effects of underexpression as well as overexpression.
For underexpression, often referred to as "gene silencing", there are two principle methods which are commonly referred to in the art as "antisense downregulation" and "sense downregulation (also referred to as "cosuppression"). Both of these methods lead to an inhibition of expression of the target gene. Other lesser used methods involve modification of the genetic control elements, the promoter and control sequences, to achieve greater or lesser expression of an inserted gene. There is no reason to doubt the operability of these methods: they are well- established, used routinely in laboratories around the world and products in which they have been used are on the market.
Gene control by any of these methods requires the insertion of a selected gene or genes into plant material which can be regenerated into plants. This transformation process can be performed via a number of methods, for example: the Agrobacterium-mεdiatsd transformation method.
In the microparticle bombardment method, microparticles of dense material, usually gold or tungsten, are fired at high velocity at the target cells where they penetrate the cells, opening an aperture in the cell wall through which DNA may enter. The DNA may be coated on to the microparticles or may be added to the culture medium. In microinjection. the DNA is inserted by injection into individual cells via an ultrafine hollow needle. Another method, viz. fibre-mediated transformation, applicable to both monocots and dicots, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride "whiskers", .and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell. In summary, then, the requirements for both sense and antisense technology are known and the methods by which the required sequences may be introduced are known. What remains, then is to identify genes whose regulation will be expected to have a desired effect, isolate them or isolate a fragment of sufficiently effective length, construct a chimeric gene in which the effective fragment is inserted between promoter and termination signals, and insert the construct into cells of the target plant species by transformation. Whole plants may then be regenerated from the transformed cells.
Bananas are a globally important fruit crop. They are not only a popular dessert fruit, but represent a vital carbohydrate staple in the tropics with as many as 100 million people subsisting on bananas and plantains as their main energy source. The cultivated dessert banana is commonly triploid, parthenocarpic and belongs to the Musa AAA genome group, eg. Cavendish subtypes. Bananas are climacteric fruits and ripening is regulated by ethylene produced by the fruit and involves numerous biochemical changes including the conversion of starch to sugars, cell wall disassembly, synthesis of volatile compounds, changes in phenolic constituents and degradation of chlorophyll in the peel. The conversion of starch to sugars is particularly striking, where starch accounts for 20-25% of the fresh weight of the unripe fruit and depending on the genetic background, can be converted almost entirely to sugars. The triploid nature of the cultivated dessert banana crop has hampered conventional methods of breeding for improved characteristics. As a result of this an enormous pool of genetic resources for enhancing postharvest characteristics of the fruit has remained untapped.
According to the present invention there is provided a method of modulating the ripening or tissue senescence process in pl.ants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID-Nos. 1 -57. regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. The said polynucleotide may be obtained from the cDNA library having the NCIMB Accession Number 40814. Further according to the present invention is a method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. The invention further provides a method as described above wherein the said polynucleotide is capable of modulating the production of pectate lyase and more specifically the polynucleotide comprises at least one of the sequences depicted in the sequence listings as SEQ-ID-Nos. 13-18.
A preferred method for inserting the said polynucleotides into plant material according to the method of the present invention, may be selected from the group comprising the Agrobacteήum, microparticle bombardment, fibre mediated or direct insertion methods. The invention further provides plants, their progeny and seed and material obtained from said plants, produced according to the method of the present invention. It is preferred that the said plants, their progeny and seed and material obtained from said plants are derived from plants of the genus Musa. The present invention also provides a vector functional in plants comprising a promoter region which is operable in plant cells, at least one of the polynucleotide sequences described above and a transcription termination sequence. The promoter may be constitutive, developmentally regulated or switchable. In addition to this the promoter may also be tissue specific or organ specific. Further provided is a banana produced via the preceding method, having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described above. The present invention also provides an improved method of controlling plant pathogens compπsing the application of an anti-pathogenic agent to plants, characterised in that plants to which the said agent is applied, are plants according to the present invention. The gene sequences of the present invention may be synthesised ab initio, using the sequence data in the sequence listing provided herewith, or isolated from a library using the standard techniques know within the art. The sequences depicted in the sequence listing or parts thereof may also be used to create oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins. To assist the isolation of these polynucleotides we have deposited with the National Collection of Industrial & Marine Bacteπa, St. Machar Drive, Aberdeen, UK, a cDNA library of the banana peel ripening related genes. The library was deposited on 9th July 1996 and has the NCIMB Accession Number 40814.
Thus, this invention is based on the identification of genes which encode proteins involved in banana ripening-related processes, specifically within banana pulp. DNA sequences according to the sequence listing or those sequences obtainable from the deposited library, may be used in the process of modifying the plant ripening characteπstics of plants and/or fruit.
By virtue of this invention banana plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characteπstics: improved resistance to damage duπng harvest, packaging and transportation due to slowing of the πpening and over-πpenmg processes; longer shelf life and better storage characteπstics due to reduced activity of degradative pathways (e.g. cell wall hydrolysis): improved processing characteπstics due to changed activity of proteins/enzymes contributing to factors such as: viscosity, solids, pH, elasticity; improved flavour and aroma at the point of sale due to modification of the sugar/acid balance and other flavour and aroma components responsible for characteristics of the πpe fruit; modified colour due to changes m activity of enzymes involved in the pathways of pigment biosynthesis (e.g. lycopene, β-carotene. chalcones and anthocyamns), increased resistance to post-harvest pathogens such as fungi. The activity of the πpemng-related proteins may be either increased or reduced depending on the characteπstics desired for the modified plant part (fruit, leaf, flower, etc). The levels of protein may be increased: for example, by incorporation of additional genes. The additional genes may be designed to give either the same or different spatial and temporal patterns of expression m the fruit "Antisense" or "partial sense" or other techniques may be used to reduce the expression of ripening-related protein. The activity of each ripening-related protein or enzyme may be modified either individually or in combination with modification of the activity of one or more other ripening- related proteins/enzymes. In addition, the activities of the ripening-related proteins/enzymes may be modified in combination with modification of the activity of other enzymes involved in fruit ripening or related processes. DNA constructs according to the invention for gene silencing, may compπse a base sequence at least 10 bases (preferably at least 35 bases) in length for transcπption into RNA. There is no theoretical upper limit to the base sequence, it may be as long as the relevant mRNA produced by the cell but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. When using genomic DNA as the source of a base sequence for transcπption it is possible to use either intron or exon regions or a combination of both.
As a source of the DNA base sequence for transcription, a suitable cDNA or genomic DNA or synthetic polynucleotide may be used. The isolation of suitable npening-related sequences is descπbed above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCIMB in Aberdeen. Sequences coding for the whole. or substantially the whole, of the appropπate npening-related protein may thus be obtained Suitable lengths of this DNA sequence may be cut out for use by means of restπction enzymes. The preparation of such constructs is described in more detail below Constructs suitable for expression of the appropπate ripening-related sequence in banana cells, may be produced using a cDNA sequence selected from the deposited library having the NCIMB Accession Number 40814 or the gene sequence as found in the chromosome of the banana plant. Recombinant DNA constructs may be made using standard techniques. In this specification modulation means an increase or decrease of the desired effect More specifically ''modulation of the npening or tissue senescence process in plants of the genus Musa" means an increase or decrease in production of a πpemng related protein resulting from the method as descπbed above For example, where an increased πpemng related protein is desired, plants may be transformed according to the method as descπbed above and those plants exhibiting the desired effect may be selected from the population of trans formants. Furthermore, it may be desirable to provide a plant with modulated ripening or tissue senescence characteristics by increasing the production of one protein and decreasing the production of another protein in the same plant. For example, a banana fruit, modified using the present method, and having decreased levels of the enzyme pectate lyase would be beneficial because pulp softening would require a substantially longer time when compared with a control. In addition to this and by increasing the levels of another ripening related protein such as an antifungal protein in the same fruit using the present method, would complement the extended life of the banana pulp with increased resistance to disease. Increase in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing a protein and thereby increasing protein levels when compared with a control plant. Decrease in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing an mR A which is capable of interfering with endogenous mRNA to such a degree that the levels of translated protein are reduced when compared with a control plant. Ripening process of plants means the process of maturing or developing . Senescence means the progressive deterioration in function of cells, tissues, organs etc.. related to the period of time since that function commenced..
Control plant means a comparable plant used for the purposes of determining modulation of the πpening or tissue senescence process effect in plants. Specifically, in plant transformations the control pl∑int is usually of the same species and variety as the material used before the transformation process and is grown in the same conditions, (usually with the transformant selection step modified in some way on the part of the control plant), as the transformed plants. More specifically the control plant may comprise an untransformed control plant or a transformed control plant providing it has not already been transformed with the same polynucleotide sequence as the plant material to be transformed. "Plant material" includes plant ceils and any other type of plant regenerable material. The following examples further illustrate the invention but are not to be construed to limit the scope thereof:
TABLE 1. Is a list of clones isolated from banana pulp and the corresponding sequence identity number .as provided in the sequence listing herein. The table also illustrates the approximate clone size, the percentage identity and, where relevant, nucleotide similarity with published sequences, based on the results obtained from comparisons with the EMBL sequence database. The table also provides, where relevant, the gene identity of those published sequences and their database accession numbers. FIGURE 1. Plant transformation vector pUN. containing the UBI polyubiquitin promoter.
FIGURE 2. Plant transformation vector pSHYN, containing hygromycin resistance gene for selection of transformed plants.
FIGURE 3. Plant transformation vector pAN. containing the banana ACC oxidase promoter.
EXAMPLE 1
Construction of a cDNA library of ripening genes 1.1 Isolation of messenger RNA
Total RNA was isolated from ripening (24 hours after ethylene treatment) banana pulp (Musa acuminata cv. Grand Nain) as described by Chang et al. Plant Molecular Biology Reporter. Vol. 1 1(2) 113-1 16 (1993). Messenger RNA was isolated from total RNA by Oligo(dT)- cellulose chromatography according to Bantle et al., Analytical Biochemistry 72, 413-427 (1976).
1.2 Synthesis of cDN A and Cloning into Vector
The first and second strands of the cDNAs were svnthesised from the messenger RNAs using a commercial cDNA synthesis kit (Catalog No. 200450, ZAP Express™ Gold Cloning kit. Stratagene Ltd. Cambridge. Ca bs, UK). Double stranded cDNAs were cloned into the ZAP Express™ vector, packaged, mixed with plating bacteria to determine titre and for library screening, following instructions of the suppliers protocol. 1.3 Screening of the cDNA library from banana pulp.
The unamplified cDNA library from ripening banana pulp was differentially screened using cDNA from unripe and ripening banana peel tissue. A proportion of the library was plated individually at low density and duplicate plaque lifts made onto Hybond N nylon filters (Amersham) according to t e manufacturer's instructions. One filter was hybridised to dCTP radiolabelled cDNA from green fruit and the duplicate filter hybridised to dCTP radiolabelled cDNA from ripening fruit. Hybridization's were at high stringency. Plaques hybridising preferentially with ripening or green radiolabelled cDNA were picked and re- plated for a second round of selection by differential screening. These clones were numbered as ripening up- or down-regulated peel clones. The clones were in-vivo excised from the ZAP express™ vector into the pBK-CMV phagemid vector using the E.xAssist™ interference-resistant helper phage, following instructions from manufacturers protocol.
1.4 Characterisation of the ripening pulp cDNA library and the ripening-related clones. The ripening cDNA library from pulp tissue were prepared with an efficiency of 3.2 x 105 plaque-forming units per microgram of cDNA. The sizes of the inserts in the peel library was 0.4 - 6.7 Kb with a mean size insert of 1.47 Kb.
From the 250 plaques used in the first screen, 73 putative ripening-related clones were obtained. These 73 clones were partially sequenced using the ABI PRISMTM Dye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq® DNA polymerise (Applied Biosystems, Warrington, Cheshire, UK) with forward pπmers specific for the pBK- CMV vector. From these, the following ripening-related clones were selected. Comparisons of these sequences in the EMBL database using GCG ('Wisconsing') software has identified homologies for the clones listed in TABLE 1 below.
EXAMPLE 2
Construction of truncated sense RNA vectors with the maize polyubiquitin promoter. A vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is svnthesised by polymerase chain reaction using synthetic pπmers incorporating BamHI restπction sites suitable for clomng between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology,
18:675-689) and a nopaline synthase 3 'end termination sequences in the vector pUN (Fig.
1.).
The truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase. pSHYN contains hygromycin resistance gene for selection of transformed plants.
After synthesis of the vector, the structure and orientation of the sequences are confirmed by
DNA sequence analysis.
EXAMPLE 3
Construction of truncated sense RNA vectors with a fruit enhanced promoter. The 1386bp Hindlll fragment containing the banana ACC oxidase promoter (UK. Application No. 9607700.3) is cloned the Hindlll site in pMSC2 (Fig. 3.) to give the vector pAN.
A vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3'end termination sequences in the vector pAN. The truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase. pSHYN contains hygromycin resistance gene for selection of transformed plants.
After synthesis of the vector, the structure and orientation of the sequences are confirmed by DNA sequence analysis.
EXAMPLE 4
Construction of an over-expression vector with the maize polyubiquitin promoter. The complete sequence of a ripening -related cDNA containing a full open-reading frame is inserted into the vectors described in EXAMPLE 2.
EXAMPLE 5
Construction of an over-expression vector with a fruit enhanced promoter.
The complete sequence of a ripening -related cDN A containing a full open-reading frame is inserted into the vectors described in EXAMPLE 3.
EXAMPLE 6
Generation of transformed Musa plants Transformed Musa plants containing the vectors are produced by the method described in Sagi et al. (1995) Biotechnology. Vol. 13 pp 481-485. Regenerated transformed plants are identified by their ability to grow on hygromycin and grown to maturity. Ripening fruit are analysed for a modulation in their ripening related or senescence characteristics. Other suitable transformation methods for banana are described in Sagi et al. ( 1994) Plant Cell Reports. Vol. 13. pp 262-266. and May et al. (1995) Biotechnology. Vol. 13 pp 486- 492.
TABLE 1
Sequence Croup Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp
SEQ-ID-NO- 1 Pulp U-U9 Isoflavone Reductase 61 .6 714 Potato (Solanum Upregulated tuberosum), X92075
c SEQ-ID-NO-2 Pulp U-U 17 Isoflavone Reductase 62.9 770 Potato (Solanum
CD C/> Upregulated tuberosum), X92075 H SEQ-ID-NO-3 Pulp U-U66 1.1 Isoflavonoid 60.0 722 Arabidopsis thailiana,
H
C Upregulated Reductase Z49777 H m SEQ-1D-NO-4 Pulp U-U 104 Isoflavonoid 60.6 696 Arabidopsis thailiana cn x Upregulated Reductase Z49777 m SEQ -ID-NO-5 Pulp U-U I 3 1.1 Beta- 1 ,3-Glucanase 58.5 585 Soybean (Glycine max),
5 Upregulated A26451 c SEQ -ID-NO-6 Pulp U-U 136 1.3 Beta-1 ,3-Glucanase 59.8 800 Barley (Hordeum vulgare fn Upregulated M91814
SEQ -ID-NO-7 Pulp U-U21 1.8 Transcriptional 54.3 31 1 Zea mays, LI 3454 σ> Upregulated Activator
Figure imgf000013_0001
Sequence Croup Clone Size Published Sequence % Identity Sequence Published Sequences
Figure imgf000014_0001
Identity no. Kb gene Identity Bp
SEQ -I -NO-8 Pulp U-U31 0.55 S- 67.0 467 Orchid (Phalaenopsis sp.),
I Ipregulated adenosylhomocysteine X79905
Hydrolase
SEQ -ID-NO-9 Pulp U-U 131 1.8 S-adenosyl L- 79.8 699 Wlieat (Triticum aestivum cn c Upregulated homocysteine L 1 1872
CD Hydrolase cn
H SF.Q -ID-NO- 10 Pulp U-U32 0.8 Beta-amylase 54.7 854 Zea mays, Z25871 c H Upregulated
H m SEQ -ID-NO- 1 1 Pulp U-U55 0.8 O-methyl transferases 60.1 223 Zea mays, L 14063 cn Upregulated
-π m m
H SEQ -1D-NO-12 Pulp U-U72 0.8 O-methyl transferase 60.6 226 Zea mays, LI 4063 Upregulated c r m* SEQ -ID-NO-13 Pulp U-U68 1.8 Pectate Lyase 57.9 394 Lilium longifloritm, Z1732 t Upregulated
SEQ -ID-NO- 14 Pulp U-U69 1.7 Pectate Lyase 66.1 516 Zea mays, L20140 Upregulated
SEQ -ID-NO- 15 Pulp U-U84 1.1 Pectate Lyase 65.8 736 Lycopersicon esculenlum, Upregulated X55193
SEQ -ID-NO- 16 Pulp U-U89 1.6 Pectate Lyase 54.8 354 Nicoliana tabacum, X671 Upregulated
Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp
SEQ -1D-N0- 17 Pulp U-U1 15 1.5 Pectate Lyase 67.3 508 Zea mays, L20140 Upregulated
SEQ -ID-NO- 18 Pulp U-U 1 17 1.7 Pectate Lyase 55.0 333 Lilium longiflυrum, Z 173 Upregulated J)
C SEQ -ID-NO- 19 Pulp U-U80 0.6 Vicilin-like storage 53.7 387 Arabidopsis thailiana, CD Upregulated protein T13642
H SEQ -ID-NO-20 Pulp U-U90 1.8 Glutamate 72.5 735 Petunia hybrida, L 16797
H
C Upregulated Descarboxylase H m SEQ -ID-NO-21 Pulp U-U92 1.8 Glutamate 71.8 740 Arabidopsis thailiana, n
X Upregulated Descarboxylase U 10034 m SEQ -ID-NO-22 Pulp U-U91 1.1 Aconitase 76.4 766 Pumpkin (Cucurbita sp.), Upregulated D29629
3D
C SEQ -ID-NO-23 Pulp U-U96 0.9 Express Sequence 69.0 452 Arabidopsis thailiana, r- m Upregulated Tag. H36910 ro SEQ -ID-NO-24 Pulp U-U 103 2 Cell Wall Invertase 66.0 567 Zea mays, V 17695 Upregulated
SEQ -ID-NO-25 Pulp U-U93 3.5 Heat Shock Protein 76.4 71 1 Lycopersicon esculentum, Upregulated cognate X54030
SEQ -ID-NO-26 Pulp U-U 125 1.8 Heat Shock Protein 71.0 662 Spinacia oleracca, L2624 Upregulated
SEQ -ID-NO-27 Pulp U-U105 0.9 Hexaubiquitin Protein 64.1 237 Sunflower (Helianthus Upregulated annuus), X57004
Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp
SEQ -ID-NO-28 Pulp U-U1 16 0.8 Polyubiquitin 81.0 625 Rice (Oryza sativa), X76 Upregulated
SEQ -ID-NO-29 Pulp U-U 120 0.9 Extensin 56.9 576 Almond (Prunus Upregulated amygdalus), X6 l\ % ω c SEQ -1D-NO-30 Pulp U-U 126 1.2 Basic Chitinase 63.2 506 Cowpea (Vigna
09 Upregulated unguiculata), X88801 V)
-i SEQ -1D-NO-31 Pulp U-U129 1.3 Beta-glucosidase 60.9 517 White clover (Trifolium
H C Upregulated repens), X56733 H m SEQ -ID-NO-32 Pulp U-U130 1.3 Chlorophyll aft 52.0 415 Rice (Oryza saliva), D006 cn Upregulated binding protein x m SEQ -ID-NO-33 Pulp U-U43 2.5 Fruit sp pp involved in 53.8 396 Tomato (Lycopersicon m
H Upregulated maturation esculentum), XI 3743
SEQ -ID-NO-34 Pulp U-U70 0.6 Root specific protein 63.6 420 Rice (Oryza sativa), L272 c Upregulated m
SEQ -ID-NO-35 Pulp U-U16 0.6 - - - No published sequence σ> Upregulated similarity
SEQ -ID-NO-36 Pulp U-U30 0.8 - • - - Upregulated
SEQ -ID-NO-37 Pulp U-U40 10 - - - - Upregulated
SEQ -ID-NO-38 Pulp U-U 108 1.1 - - - - Upregulated
Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences
Figure imgf000017_0001
Identity no. Kb gene Identity Bp
SEQ -ID-NO-39 Pulp U-U 128 2.2 - - - - Upregulated
SEQ -ID-NO-40 Pulp U-D4 1.3 Granule Bond Starch 62.3 621 Cassava (Manihol
Down Synthase esculenta), X74160 c regulated
CD α> SEQ -ID-NO-41 Pulp U-D10 1.3 Starch Synthase 68.8 506 Pea (Pisum sativum),
H Down X88789 H regulated m SEQ -ID-NO-42 Pulp U-D13 1.3 Starch Synthase 67.4 454 Pea (Pisum sativum),
(/> Down X88789 x m regulated m
H SEQ -ID-NO-43 Pulp U-D66 1.3 Granule Bond Starch 64.1 669 Cassava (Manihol
Down Synthase esculenta), X74160 c r- regulated m ι SEQ -ID-NO-44 Pulp U-Dl l l 1.3 Starch Synthase 66.4 655 Cassava (Manihol σ>
Down esculenta), X74160 regulated
SEQ -ID-NO-45 Pulp U-D1 12 2.3 Granule Bond Starch 68.4 196 Potato (Solanum
Down Synthase tuberosum), X58453 regulated
Figure imgf000017_0002
Figure imgf000018_0001
Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp
Figure imgf000019_0001
SEQ -ID-NO-53 Pulp U-D65 0.9 Extensin 56.7 556 Cowpea (Vigna
Down unguiculatά), X91836 regulated
SEQ -ID-NO-54 Pulp U-D84 0.8 Extensin 58.3 492 Cowpea (Vigna
CΛ Down unguiculata), X91836
C CD regulated 09 •H SEQ -ID-NO-55 Pulp U-D75 1.2 Wali 7 74.4 703 Wheat (Triticum aestivum
H
C Down L28008 H m regulated cn SEQ -ID-NO-56 Pulp U-D83 1.2 Wali 7 72.8 743 Wheat (Triticum aestivum x Down L28008 regulated
31 SEQ -1D-NO-57 Pulp U-D64 2.3
C r-. Down m regulated
SEQUENCE LISTING
(1) GENERAL INFORMATION:
(i> APPLICANT:
(A) NAME: ZENECA LIMITED
(B) STREET: 15 STANHOPE GATE
(C) CITY: LONDON (D) STATE: LONDON
(E) COUNTRY: UNITED KINGDOM
(F) POSTAL CODE (ZIP): W1Y 6LN
(ii) TITLE OF INVENTION: GENETIC CONTROL OF FRUIT RIPENING (iii) NUMBER OF SEQUENCES: 57
(iv) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible
(C) OPERATING SYSTEM: PC-DOS/MS-DOS
(D) SOFTWARE: Patentln Release #1.0, Version #1.30 (EPO)
(v) CURRENT APPLICATION DATA: APPLICATION NUMBER: GB PPD
(2) INFORMATION FOR SEQ ID NO: 1:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 785 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS : single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U9
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: CGGCACGAGG AAAAACTANG TGAGAANGAG ATAATCGTTG ACCGAGGNAG AGAATGGCGA 60
GCGAGAAGAG CAAAATCCTG ATCATCGGGG GCACCGGGTA CATCGGCAAG TTCATCGTGT 120
TTGCGAGCGC CAGGTTAGGT AACCCTACCT TCGCTCTCGT CCGGAGCACC ACCGCCCCCG 180
CCGGCCAACC CGAGAAGGCC AAGCTCCTGA GCGACTTCCA GGCCGCCGGC GTCACCCTCG 240
TCCAGGGGGA TATNTATAAC CACGAGAGTC TGGTTAAGGC GATCAAGCTG GTGGATGTGG 300 TCATCTCCCC CGTCGGCTTC GGGCANCTGA NTGATCAGAC CAAGATCATC GACGCCATCN 360
AANAAGCCGG AGGACACATC AAGAGGTACC TTCCATCGGA GTTTGGCAAC GACGTANACC 420
GAAGCCATGC TGTGGAGCCA GCAAAGTCTA CCTTTGTCGT CAAGCAACAA ATCANAAGGG 480
CTGTTGAGGC ATCGGGTNTC CCTTACACCT TTGTATCTTC C.AACTTTCTT CGGTGGGTNT 540
TTCCTCCCGG TATTATGACA GGCAGGAGCC ACTGGTCCTC CCACGGACAA GGTTGTCATC 600 TTAGGTNACG GGAACNCAAA ACGATCTTCT CNATGAANAC GACTTCCGGA CATCCCATTT 660
ANTCTTGGAT GATCCATAAC CTGAACCAGG TTCTATTTCT TAAAACTTCC NCCACTTCTT 720
NTCTCTTANC ANCTCNTTTC CCTCTTGGGA AAAAAATGTC NNCTANACTT CNAAAAGGGT 780
TTANT 785 (2) INFORMATION FOR SEQ ID NO: 2:
(l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (11) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U17
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: CGCGCACGAG GAAGAAAACT AGGTGAGAAN GAGATAATCG TTGACCGAGG NAGAGAATGG 60
CGAGCGAGAA GAGCAAAATC CTGATCATCG GGGGCACCGG GTACATCGGC AAGTTCATCG 120
TGTTTGCGAG CGCCAGGTTA GGTAACCCTA CCTTCGCTCT CGTCCGGAGC ACCACCGCCC 180
CCGCCGGCCA ACCCGAGAAG GCCAAGCTCC TGAGCGACTT CCAGGCCGCC GGCGTCACCC 240
TCGTCCAGGG GGATATATAT AACCACGAGA GTCTGGTTAA GGCGATCAAG CTGGTGGATG 300 TGGTCATCTC CCCCGTCGGC TTCGGGCANC TGANTGATCA GACCAAGATC ATCGACGCCA 360
TCAAAGAAGC CGGAGGACAC ATCAAGAGGT ACCTTCCATC GGAGTTTGGC AACGACGTAN 420
ACCGAAGCCA TGCTGTGGAG CCAGCAAAGT CGACCTTTGT CGTCAAGCAA CAAATCANAA 480
GGGCTGTTGA GGCATCGGGT ATCCCTTACA CCTTTGTATC TTCCAACTTC TTCGGTGGGT 540
NTTTCCTCCC GGTATTANGA CAGGCAGGAG CCACTGGTCC TCCCACGGAC AAGGTTGTCA 600 TCTTANGTGA CGGGAACACA AAAGCGATCT TTCTCAATGA ANACACATCC GGACNTNCAC 660
NATTAAAGCA GTGGATGATC CGAAAACCTG AACANGTTCT ATATCTGAAA CCTTCCGCCA 720
CTCTTGTCTC ATNACAACTC ATTTCCCTCT GGGAAAAAAA NGTCNGCAAA ACTCCNAAAA 79C
GGTCTACTTC CCGGAAGAAA AATCTGAANC ANA 813
(2) INFORMATION FOR SEQ ID NO: 3: (l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 746 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(li) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U66
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3:
GGCACGAGGG GAAAA.GGAGA TNATCGTTGA CCGAGGAGAG AATGGCGAGC GAGAAGAGCA 60
AGATCCTGAT CATCGGGGGC ACGGGGTACA TCGGCAAGTT CATCGTGTTT GCGAGCGCCA 120 GGTTAGGTAA CCCTACCTTC GCTCTCGTCC GGAGCACCAC CGCCCCCGCC GGCCAACCCG 130 AGAAGGGCAA GCTCCTGAGC GACTTCCAGG CCGCCGGCGT CACCCTGGTC CAGGGGGATA 240
TATATAACCA CNAGAGTCTG GTTAAGGCGA TCAAGCTGGT GGATGTGGTC ATCTCCCCCG 300
TCGGCTTCGG GCAGCTGAGT GATCAGACTA AGATCATCGA CGCCATCAAA GAAAGCCGGA 360
GGACACATCA AGAGGTACCT TCCATCGGGA GTTTGGCAAC GACGTANACC GAAGCCATGC 420
TGTGGAAGCC ANCAAAGTCG ACCTTTGTCG TCAAGCAACA AATTANAAGG GCTGTTGAGG 480
CATCGGGGAT CCCTTACACC TTTGTTATCT TCCAACTTCT TCGGTGGGTA TNTCCTCCCC 540
GTATTGGGAC AGGCANGAAC CACTGGTCCT CCCCACGGAC AAGGTTGTCN TCTTAGGTGA 600 ACGGGAACAC CAANGCGATC TTTCTCAATG AAAGACAACT CGGGACATNC CCNATTTAAC 660
CANTGGATGA TCCNANAACC TGAACAAGGT CTATTTCTGA AAACTTCNCC ATCTTCTTTT 720
TCTCATAACG AACCCNTTTN CCTCTT 746 (2) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 795 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U104
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:
GGCACGAGGA AANGAGATAA TCGTTGACCG AGGCAGAGAA TGGCGAGCGA GAAGAGCAAA 60
ATCCTGATCA TCGGGGGCAC CGGGTACATC GGCAAGTTCA TCGTGTTTGC GAGCGCCAGG 120
TTAGGTAACC CTACCTTCGC TCTCGTCCGG AGCACCACCG CCCCCGCCGG CCAACCCGAG 180 AAGGCCAAGC TCCTGAGCGA CTTCCAGGCC GCCGGCGTCA CCCTCGTCCA GGGGGATATA 240
TATAACCACG AGAGTCTGGT TAAGGCGATC AAGCTGGTGG ATGTGGTCAT CTCCCCCGTC 300
GGCTTCGGGC AGCTGAGTGA TCAGACCAAG ATCATCGACG CCATCAAAGA AGCCGGAGGA 360
CACATCAAGA GGTACCTTCC ATCGGGAGTT TGGCAACGAC GTTANACCGA AGCCATGCTG 420
TGGAGCCAGC AAAGTCNACC TTTGTCGTCA AGCAACAAAT CANAAGGGCT GTTGANGCAT 480 CGGGTNTCCC TTACACCTTT GTATCTTCCA ACTTCTTCGG TGGGTATTTC CTCCCGGTAT 540
TANGACAGGC AGGACCACTG GTCCTCCCCA CNGACAAGGT TGTCNTCTTA GGTGACNGGA 600
ACACAAAANC ATCTTTCTCN ATGAAGACAA CTCCGGACAT ACNCNATTAA AGCNGTGGAT 660
GATCCCAAAA CCTGAACAAG TTCCTATNTC TTGAAAACTT TCCCGCCCAA CCATCCTTTN 720
GTTCTCCCNT TAAACCNAAC CTCCATTTTC CCCTCCTTGG GGAAAAAAAA AAGGTCCGGC " 90 NAANAACTTC CNAAA 795
(2) INFORMATION FOR SEQ ID NO: 5: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 797 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: CDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U13
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:
CGGCACGAGG NAGAACCTTT TGACAGAGTT GTTGTCATGG CAACAAAAGC TTCTCTCTCC 60
ATAAAAGGCT TTGCCTTGCT GGTTTCAGTC CTTGTAGCAG TTCCAACAAG AGTGCAATCG 120 ATTGGTGTCT GCTACGGCAT GCTCGGCAAC AATCTTCCCC CGCCCAGCGA GGTGGTCAGT 180
CTCTACAAAT CCAACAACAT CGCGAGGATG AGACTCTACG ATCCAAACCA AGCCGCCCTG 240
CAAGCCCTCA GGAACTCCAA CATCCAAGTC CTGTTGGATG TCCCCCGATC CGACGTGCAG 300
TCACTGGCCT CCAATCCTTC GGCCGCCGGC GACTGGATCC GGAGGAACGT CGTCGCCTAC 360
TGGCCCAGCG TCTCCTTTCG ATACATAGCT GTCGGAAACG AGCTGATCCC CGGATCGGAT 420 CTGGCGCAGT ACATCCTCCC CGCCATGCGC AACATCTACA ATGCTTTGTC CTCGGCTGGC 480
CTGCAAAACC AGATCAAGGT CTCGACCGCG GTCGACACGG GCGTCCTCGG CACGTCCTAC 540
CCTCCCTCCG CCGGCGCCTT CTCCTCCGCC GCCCAGGCGT TACCTGANCC CCATCGTGCA 600
GTTCTTGGCG ANTAACGGAA CGCCGCTCCT GGTCAATGTG TACCTTATTT TAACTACACC 660
GGCAACCCGG GANAGATCTC GCTGCCTACN CCCTGTTCAC GGCCNCGGGG TCTCNTGCAG 720 GATGGGCGAA TTCCNCTATC ANAANCTGTT CANTCCATCT TCNAAACCGG TCTTCCCGGG 780
CTGGAAAAAA TTGGAAG 797
(2) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 792 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U136
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:
GGCACGAGGA GAACCCTTTT GACAGAGTTG TTGTCATGGC AACAAAAGCT TCTCTCTCCA 60 TAAAAGGCTT TGCCTTGCTG GTTTCAGTCC TTGTAGCAGT TCCAACAAGA GTGCAATCGA 120
TTGGTGTCTG CTACGGAATG CTCGGCAACA ATCTTCCCCC GCCCAGCGAG GTGGTCAGTC 180
TCTACAAATC CAACAACATC GCGAGGATGA GACTCTACGA TCCAAACCAG GCCGCCCTGC 240 AAGCCCTCAG GAACTCCAAC ATCCAAGTCC TGTTGGATGT CCCCCGATCC GACGTGCAGT 30C CACTGGCCTC CAATCCTTCG GCCGCCGGCG ACTGGATCCG GAGGGAACGT CGTCGCCTAC 360
TGGCCCAGCG TCTCCTTTCG ATACATAGCT GTCGGAAACG ANCTGATCCC CGGATCGGAT 420
CTGGCGCAGT ACATCCTCCC CGCCATGCGC AACATCTACA ATGCTTTGTC CTCGGCTGGC 480
CTGCAAAACC AGATCAAGGT CTCCGACCGC GGTCGACACG GGCGTCCTCG GCACGTCCTA 540
CCCTCCCTCC CGCCGGNGCC TTCTCCTCCG CCGCCCAAGC GTNCTGANCC CCATCGTGCA 600
NTTCTTTGGC GAAATAACGG ANCGCCGCTC CCNGGTCCAT GTTTTTCCCT TATTTTAACT 660
ACNCCGGCAA CCCNGGAAAG ATTTCGCTGC CCTTACGCCC CCTGGTTTCC NNGGGGCTTC 720 CCGGGCGTTT CTTCCTTTGC CAGGGANTNG GGGCGAATTC CCNNCTTTTC CANAAACCTT 780
GTTCCAACNC CC 792
(2) INFORMATION FOR SEQ ID NO: 7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 855 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U21
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:
CGGCACGAGT CTCTCTCTGT CTCTCCGTCG TCTCGTTGTC TGTTCGTTAG GGCTTGCGAT 60
CGCCACCGGT CGCGAGGGTT GGAGCCATGG AATTCTTGCG GTTTGGGGCT GAGGTGGCGG 120
CAGGAGAGGA AGCCGCGACG GGGTACTGGA TGCGATGGCA GACGCTCGTC TGCGCTCTGA 180
TCGTCGTCGC CCCGGCGGTC GCCGCGGTGG TTGTCGCGGC GCGGGCGCCG GCGCCGGCGC 240 GACCGCTCAG GGCCGTCGAT CTCTGGGCGC CGTGCTGGGC CGGGATGCAC CCGGCCTGGC 300
TTCTCGCGTA CCGCGGCTTC NTGTTCTTGG CCATGGCGTG GCTACTCTTC CANATGATTC 360
TGTTTCGCGG ATTCTCCGCG TTCTACTTCT ATACTCAGTG GACGTTTGCC TTAGTCATTG 420
TCTATTTTGC GATCGCAACC ATTATATCCG CCCATGGCTG CTGGCTTTAC TCAAAGAGAA 480
GTATTATGCC AGATCAGGAG GTCAACAGAT TCCTAAATGG TGGTTTTGAG CANAATAGTC 540 CTATGACTCT ACCTTTGAGG ACCAACAAAA ATATGAATGT TATAAGATTG CAAAGCTATC 600
NTGAACAGGA NGCTGATGAA NAAAAANCTG GATTTTGGGG TCCTGCTATG CNACTNGTCT 660
ATCAAAACCA TTGCANGTGC TGTAATTGTT GAAANACATT GTATTTTGGG NTCNTNCTAA 720
TACCATATTT GTCTTCTATA AATTTCAGGC TAAATGCTAT AATGGGCTGC ATGCATTCTC 780
TTAATGCTGT GTTTCTTCNA ATCCAACACT TTTCTCAATA ACCTGCCATT CCCNTNTTTC 840 NAATGGCATT TTTGC 855
(2) INFORMATION FOR SEQ ID NO: 8:
Ii) SEQUENCE CHARACTERISTICS: (A) LENGTH: 722 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U31
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:
CGGCACGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 60
GAGAGAGAGC CTAGACCGGC ATCNTCGTGC TTGCCGAGGG GCGGCTTATG ANTCTTGGTT 120
GTGCCACTGG GCATCCCAGC TTCGTCNTGT CCTGCTCCTT CACCANCCAN GTGATNGCAC 180 NACTGGANTT ATGGANGGAA ANGGCGACCG GCNNGTACGA NAAGAANGTC TATGTGCTGC 240
CCANGCNTCT GGATGAGAGG GTGGCNGCGC TCCNCCTCGG CAAGCTGGGT GCCNCGCTCA 300
CCNAGCTTAC NCCNTCGCGG GCTGATTACA TTAGCGTCCC GATCGAGGGA CCCTACANGC 360
CTGCTCACTA CNGGTATTNG GGTTGCTTNT GCNAGAGACG ATGATNATAN NTCGGAGCAC 420
TGGCNTTTTC GACTANNAGT TTGACCGATG GCTATGTTCG TTTTGCTTTT CACCTTTTGT 480 CTTCCCATCT TTGCTGGTTC ACCTATGGAC GTTTGTTCCA TTTGGATGTN NTGAGAAATG 540
CTGATGGCAT TTTCGGAAAN AAAAANATNT AAAATCNCGA GAGTTCTTCT ANAGCGGCCG 600
CGGGCCCNTC NNTTTTCCCC CGGGTGGGGT ACCANGTTTG TTGTNCCCNT TCCCCCTNTT 660
TGAGTCCTTT TACTNCCCCT GGCCGTCGTT TTATACNTCG TTGACTGGGA ANACCCTGCC 720
NT 722 (2) INFORMATION FOR SEQ ID NO: 9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 793 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U131
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9:
GGCACGAGCC TTGCCCAACT CCTTCGATTC GTTCCAACGA TCGGAGAATG GCGCTGCTGG 60 TGGAGAANAC GTCGACGGGA CGCGAGTACA AGGTGAAGGA CCTTTCTCAG GCTGACTTCG 120
GCCGCCTCGA GATCGAGCTG GCGGAGGTGG AGATGCCGGG CCTCATGGCG TGCCGCGCCG 180
AGTTCGGGCC CGCCAAACCT TTCGCCGGCG CCCGCATCTC CGGCTCCCTC CACATGACCA 240
TCCAGACCGC CGTCCTCATC GAGACCCTCA CCGCCCTCGG TGCTGAAGTC CGGTGGTGCT 300
CCTGCAACAT CTTCTCCACC CAGGACCACG CCGCTGCCGC CATTGCCCGC GACTCCGCCG 360 CCGTCTTCGC CTGGAAGGGA GAGACCCTCG CCGAGTACTG GTGGTGCACC GAGCGATGCC 420 TCGACTGGGG CCCCAACGGT GGCCCCGACC TCATCGTCGA CAAAGGGGGT GACGCCACTC 480
TCCTCATCCA CGAAGGCGTC AAGGCTGANG AAGANTACGA AAAAGACCGG CAAGCTGCCC 540
GATCCGGCCT CCACGGAAAA CGCCGAATTC CAAATCGTGC TGGGGATTAT CCGCNANCGG 600
CTCCAGGTCN AACCCCNAAA ATTACCGCCC AGATNAAAGA ACCCTCCTCC GCTTTTTGAA 660
GAAAACANCN CTGGCGTTCA NCGAATCTAC CANATGCCAG GGCCANCNGG GGCCCCTTGC 720
TTCTTTCCCC CCNCCCANTC NAACNTTCCN ACGAATTCCC GTTTTACCCN AAAAACCAAN 780
TTTTGAACAA CCT 793 (2) INFORMATION FOR SEQ ID NO: 10:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 847 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U32
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:
CGGCACGAGG CGACGCCGGC CTTCGCCTCC GCGTCTCCCT CCACCTCCAC TGCCACCGCC 60 GCCCTCGCCT CCCCCTCCCC AAATCGGTCG ACTCCGCCGC CGCTACAGAC CCCGACATCC 120
TATTCACTGA CCGCGCCGGC CGCCGCCGTG CCGACTGCCT CTCCTTCGCT GTCGACGACC 180
TCCCTGTCCT CGACGGCAGG ACCCCGATGG AAGCCTACGA GGAGTTCTTC CGGAGCTTCC 240
GCCTCGCCTT CGCTGACTTC TTTGGTTCCG TCATTACGGA TATCACGATC GGTCTCGGGC 300
CAAATGGCGA GCTCCGTTAC CCTTCGTTTC CTCCCACTGG AAGCAATCGT TTCACCGGTG 360 TAGGAGAATT CCAGTGTTAT GACAAATACA TGCTTGCCGA TCTCAAACGA CACGCGGAGG 420
AAACCGGCAG CCCATTATGG GGCCTCTCTG GTCCTCACGA TGCCCCTGGG TACAACCAGT 480
CTCCGGACTT CGGCAACTTT TTCAAGGACC ATGGCGGCTC CTGGGANACA CCCTACGGGC 540
AATTCTTCCT CTCCTGGTAC ACCGGCAAGC TCCTGTCTCA CGGTGACGGC CTGCTCTCGG 600
TCGCGTCAGA AGTATTGGTG ATTTGCCTGT CGCGCTCTCG GGCAAGGTTC CACTTTCTGC 660 ATTTGTTGGC AACGAAACTC GGTCGCGCCC GTCTTANCTT GAAGGCCGGG TTCTNTNACA 720
CTTGACGGNA NAANAACNGG TTTCAAGGAT GTTGGCTAAN ATCTCCCGAA ANCNTCCTGC 780
ANCATGATTC ATCCCGGGCN TGGACTTCTC NGAAGGGGAA CANTCCTCAG GGTGTTCCGG 840
TCTGCCC 847
(2) INFORMATION FOR SEQ ID NC : 11: u) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 822 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(11) MOLECULE TYPE: cDNA (vu) IMMEDIATE SOURCE: (B) CLONE: U-U55
(xi ) SEQUENCE DESCRIPTION: SEQ ID NO: 11:
GGCACGAGGC ATGGAGAACC CTGTTGCGAT GGGCATCATG GAGGAGCTTG AGGCCCAGAG 60
CGAGGTGTGG AACCACATTT TTAGATTCAT CACCTCCATG TCCGTCAAGT GCGCGGTGGA 120
GCTCAGGGTC CCCGACGCCA TCCACGCCCA CGGCGGCAAC GCAACTCTCC CTCAGCTGGC 180
CGCCGCCCTC AGCCTCCCTC CCGCCAAGCT CGCCGACCTC CGACGCCTCA TGCGCATGCT 240
GGTCCACGCC GGCTGCTTCG CCAAGCAGGA AGACNACGTG TACGCCCTCA CGCCATGGTC 300 GAGGCTCCTG NTGAGCTCCG AGCACACCGC GGTAGCCCCG TTCGTGGTGT GGATGCTCCA 360
CCCGCTCATG GTGCAGTCGT GGCACTCGCT GGGGGCGTGG TTCCACGGGA GGGCGCCCAC 420
CCCCTTCGCC GCAACCCACG GGAAAGGGGA TCTTCGAGAC NACACNCNAA CAGCCGGGGT 480
TCGCGGCCGT TCTTCANCNA NGCNATGGCG AACGACTGTC GGCTGGTGGG AAAGGTGTTG 540
GTAAANAACA TNCGGATGTG TTGGANGGAN CNCNGGTCCA TNGTGGAAGT TGGTGGCGCA 600 CCGGCACCCT TTTNGGCCAT TGTTGGCCGA AGGCTTTCCC GCCAACATNA AATNGCACCG 660
TTCTCCNANT TTGCCCCACT TTTCTGGCCN CNGCCGCCGG GGCCCCCNCN NACCNAAACA 720
ACTTGGATGT TTTTNGGGGG AANACATNTT CCAACATTTT ACANCCGCNC GACNTTTTTT 780
ACNCAATTGG TTCCNGGCNC NAATGGAAAT ANTNCTGNAT TT 822
(2) INFORMATION FOR SEQ ID NO: 12: (l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 743 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U72
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:
GGCACGAAGA GCCCTGTACC GGANAGCATG GAGAACCCTG TTGCGGTGGG CATCATGGAG 60
GAGCTTGAGG CCCAGAGCGA GGTGTGGAAC CACATTTT A GATTCATCAC CTCCATGTCC 120 GTCAAGTGCG CGGTGGAGCT CAGGGTCCCC GACGCCATCC ACGCCCACGG CGGCAACGCA 180
ACTCTCCCTC AGCTGGCCGC CGCCGTCAGC CTCCCTCCCG CCAAGCTCGC CGACCTCCGA 240
CGCCTCATGC GCATGCTGGT CCACGCCGGC TGCTTCACCA AGCAGGAANA CNACGTGTNC 300
GCCCTCACGC CATGGTCGAN GCTCCTGGTG AGCTCCGANC ACACCTCGGT GGCCCCGTTC 360
GTGGTGTGGA TGCTCCACCC GCTCATGGTC CAGT GTGGC ACTCMCTGGG GGCGTGGTTC 420 CACGGGAAGG GCGCCCACNC CCTTCNCCNC NACCCNCGGG AAGGGGATCT TCCANACGAC 480 ACNCGAACAT CCGGGGTTCG CGGGCGTCTT CAACTANGCG ATGGCGAACG ACTGTCNGCT 540
GGTGGGAAAN GTNTTGGTAA AAAAACCTTC CGGAAGTGTT NGANGGAACC CCGTCCAATN 600
GTGGAANTTG GTGGCNGCCG GCACCCTTNC GGGCNATCGT TGTCGAAGNC CTTCCCNCAC 660
NTTANATTNC ACCGTCCTCC AATTNTCCCC ANTTTCTTTN GCCGCNGGNN NCGGGCCCCN 720
GGTNANCCNA AANCNTTTNT TTT 743 (2) INFORMATION FOR SEQ ID NO: 13:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 806 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U68
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:
TGTTCTCTCC TTCGATCACA TCTTTTTGCT CTGGGAAACG TGAGAGGTGA GACGAGGGCG 60
GCAATGACGG CGGGTTTAAG ATGGATTCCT CCTCTGCTTC TTCTTCTTCT GGGCTTCCTG 120
CTGGTTTTGA ACGGAGGTCG GGGGTGGATT GGAAGCGAGA GGTCCTCTGG CTCGAGGAAT 180 GGCGGAGCAT CGCGGAGGAG CTTGAGAGAG GCCTCCGCGA ACGCGACCAG CGCCGATGCT 240
TCCTTGGAAG AGAGGGCTGT AACCCGGGCA GCAGAAGCCG CAGTCGACGA CCCCGAGGAG 300
GTTGCTTCGA CGGTCCTGAT GACCATAATC AACAGCACGG CTCGCAGATC TCTTGGTTAT 360
CTGTCGTGCG GTTCAGGCAA CCCGATCGAC GACTGCTGGC GGTGCGACCC TGATTGGCAT 420
GTCAACAGAA AAAAGCTCGC TGACTGCGGC ATTGGCTTTG GACGCAACGC NATANGTGGC 480 CGCGACGGGG AATTTGTTCG TTGTGACAGA CTCCGGGGAC NATGATCCCC GTGAATCCTC 540
GCCCGGGAAC ACTTANATAC CCCGTCNTCC AANGAAGTTG CCCCCTCTGG GATCCCCTTT 600
AAACNCNAAN TGGAAATCTC NCTCNANGGA AGAACTCNTT ATGAACAGCT TTAANACNAT 660
CGATGGACNC NGTGTCCTCG TCCACATTGC CAATGGCGCC TGCNTCACCA NCCAATTTCN 720
TCCCCAACNT CTTCTTCCNT NGCCTCCNCT CCCCAATGCA ACCCNCCGGG AATTCCTNGT 780 CCCCNCTCCC CTTCTCCTAT GGATNG 806
(2) INFORMATION FOR SEQ ID NO: 14:
(l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 637 base pairs
(B) TYPE : nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown 'n, MOLECULE TYPE: cONA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U69 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:
GGCACGAGGG AGAACAAAAA TGGCAGCATT CTTGTTCTTC CTCACCATCG CAGCCTTCAC 60
TGCCCCCATC TACTCTTCTC GTGCACCCTT AACGTCGGCA GCTGTCCGCG ACCCTGAATT 120
AGTAGTACAG GAAGTACAAA GAAGCTTGAA CGTGTCGCGG CGGCGACTGG GCTACTTGTC 180 ATGCGGCACC GGCAATCCGA TCGACGACTG CTGGCGGTGC GACCCTGACT GGGCTGACAA 240
CCGGCAGCGG CTCGCGGACT GCGCCATCGG GTTCGGGAAG AACGCGATTG GGGGCAGGGA 300
CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACNAC CCCGTCAATC CGAAGCCGGG 360
CACGCTCCGG TACGCCGTCA TCCANGAGGG AGCCGCTGTG GATCATCTTC AAGCGCGACA 420
TGGTCATCCA GCTGAAGGAA GGAGCTCATC ATGAAACTCC CACANAGACC ATCGACGGCC 480 GGGGCGCCAG CGTCCACATC TCCGGGGGGC CGTGCATCAC CACCAGTNCG TCNCCAACAT 540
CATCATCCAC GGGCGTCCAC TTCCACNAAT GCAANCAGGG CGGGGAACGC CTTACGTTCG 600
CGACTCCCCC AGGGCACTAC GGGTGGCGCA CCGGTGTCCG AACGGCNANG GGGTTTTCAT 660
CTTCCGCGGG AACCACTTCT GGGGTCA 687
(2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 741 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U84
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:
TCCACATTGC CAATGGCGCC TGCATCACCA TNCAGTACAT CACCAACGTC ATCATCCATG 60
GCCTCCACAT CCACGACTGC AAGCCCACCG GGAATGCCAT GGTCCGCAGC TCTCCTTCTC 120 ACTATGGATG GAGAACCATG GCTGATGGGG ATGCCGTTTC CATTTTCGGC TCCAGCCACA 180
TTTGGGTGGA CCACTGCTCT CTGTCCAACT GCGCCGATGG ACTTGTCGAT GCCGTCATGG 240
GCTCCACTGC CATTACGGTC TCCAACAATT ACTTCACCCA CCACAATGAG GTCATGCTTT 300
TGGGACACAC TGATTCTTAT GCAAGGGACA GCATCATGCA AGTAACGATC GCATTTAACC 360
ATTTTGGTGA AGGTCTGATT CAGAGAATGC CCAGGTGCAG GCATGGCTAC TTCCACGTGG 420 TAAACAATGA CTACACGCAC TGGGAGATGT ACGCCATTGG CGGTAGCGCG AATCCAACGA 480
TCAACAGTCA AGGCAACCGA TACCTTGCGC CGACCAATCC ATTTGCAAAG GAAGTAACAA 540
AAAGGGTGGA CACAGATCAA AGCACGTGGA AAAACTGGAA TTGGANGTCN GAAGGTGACC 600
TGCTTCTGAA TGGTGCTTTT TTCACCCTTC CGGTGCANGG GCTTTCACCC ANCTACGCAC 660
GGGCCTCCAC TTTGGGGGCA ACCCTCTTCC TTNGTTGANA CACTGACTCT GATGCTGGGG 720 TCCTGTTTTG CCAATTCGGN C 741 (2) INFORMATION FOR SEQ ID NO: 16:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 727 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: Single
(D) TOPOLOGY: unknown
(ll) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U89
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:
GGCACGAGTT CTCTCCTTCG ATCACATCTT TTTGCTCTGG GAAACGTGAG AGGTGAGAAG 60
AGGGCGGCAA TGACGGCGGG TTTAAGATGG ATTCCTCCTC TGCTTCTTCT TCTTCTGGGC 120
TTCCTGCTGG TTTTGAACGG AAGTCGGGGG TGGATTGGAA GCGAGCGGTC CTCTGGCTCG 180 AGGAATGGCG GAGCATCGCG GAGGAGCTTG AGAGAGGCCT CCGCGAACGC GACCAGCGCC 240
GATGCTTCCT TGGAAGAGAG GGCTGTAACC AGGGCAGCAG AAGCCGCANT CGACGACCCC 300
GAGGAGGTTG CTTCGACGGT CCTGACGACC ATAATCAACA GCACGGCTCG CAGATCTCTT 360
GGTTATCTGT CGTGCGGTTC AGGCAACCCG ATCGACGACT GCTGGCGGTG CGACCCCGAT 420
TGGCATGTCA ACAGAAAAAA GCTCGCTGAC TGCGGCATTG GCTTTGGCCC GCAACGCGAT 480 ANGTGGCCGC CACNGGGANT TGTNCNTTGT TGACAGACTC CGGGGACGAT GATCCCGTGA 540
ATCCTCCCCC GGAACACTTA ATATTCCCCG TCATCCCAGA NCTNCCCCTC TGGATCNCCT 600
TTTAAACACG ACATTGGAAA TCNCCCCCNG GAAGAACTCC TTTNTAACCG CTTTNNAACA 660
TCNATGGACN CGTGTCCACN TTCCACNTTT GCCAATGGCG CCTGCCTCNC CANCCNATTT 720
AATCNCC 727 (2) INFORMATION FOR SEQ ID NO: 17:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 842 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(11) MOLECULE TYPE: cDNA
(v i) IMMEDIATE SOURCE: (B) CLONE: U-U115
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:
GGCACGAGGG AGAACAAAAA TGGCAGCATT CATGTTCTTC CTCACCATCG CAGCCTTCAC 60 TGCCCCCGTC TACTCTTCTC GTGCACCCTT AACGTCAGCA GCTGTCCGCG ACCCTGAATT 120
AGTAGTACAG GAAGTACAAA GAAGCTTGAA CGTGTCGCGG CGGCGACTGG GCTACTTGTC 180
ATGCGGCACC GGCAATCCGA TCGACGACTG CTGGCGGTGC GACCCTGACT GGGCTGACAA 240
CCGGCAGCGG CTCGCTGACT GCGCCATCGG GTTCGGGAAG AACGCGATTT GGGGCAGGGA 300 CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACGAC CCCGTCAATC CGAAAACGGG 360
CACGCTCCGG TACGCCGTCA TCCAGGAGGA GCCGCTGTGG ATCATCTTCA AGCGCGACAT 420
GGTCATCCAG CTGAAGGAGG AGCTCATCAT GAACTCCCAC AAGACCATCG ACGGCCGGGG 480
CGCCAGCGTC CACATCTCCG GCGGGCCGTG CATCACCACC AGTACGTCAC CAACATCATC 540 ATCCACGGCG TCCACATCCA CGAATGCAAG CAGGGCGGGA ACGCGTNCGT TCGCNACTCC 600
CCAAGGGCAC TACGGGTTGG CGCACNGTGT TCNGACGGCG ACGGGTGTCC ATCTTCCGCG 660
GGAACCACTC TGGGTCCAAC CACTGCANCG CTTGTTCCAA CTTGGCCCAC CNAANTGGGC 720
CTTCCNTCCC AACCCCCATT TCCTTGGGAT TNCCCCCCGC CAATTCCCCC ATTTTCCAAC 780
NAACTTNCTT TGAACCCNCC CNTGAANAAG GTCCTTGCTG CTGGGTCCCA CCAACAATTT 840 NA 842
(2) INFORMATION FOR SEQ ID NO: 18:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 724 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U117
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: GGCACGAGTC TCCTTCGATC ACATCTTTTT GCTCTGGGAA ACGTGAGAGG TGAGACGAGG 60
GCGGCAATGA CGGCGGGTTT AAGATGGATT CCTCCTCTGC TTCTTCTTCT TATGGGCTTC 120
CTGCTGGTTT TGAACGGAGG TCGGGGGTGG ATTGGAAGCG AGAGGTCCTC TGGCTCGAGG 180
AATGGCGGAG CATCGCGGAG GAGCTTGAGA NAGGCCTCCG CGAACGCGAC CAGCGCCGAT 240
GCTTCCTTGG AAGACAGGGC TGTAACCAGG GCAGCAGAAG CCGCAGTCGA TGACCCCGAG 300 GAGGTTGCTT CGACGGTCCT GACGACCATA ATCAACAGCA CGGCTCGAAN ATCTCTTGGT 360
TATCTGTCGT GCGGTTCAGG CAACCCGATC GACNACTGCT GGCGGTGCGA CCCCGATTGG 420
CATNTCAACA GAAAAAAGCT CGCTGACTGT GGCATTGGCT TTGGCCGCAA CCCGATANGT 480
GGCCGCGACG GGGANTTGTN CGTTGTGACA GACTCCGGGG ACGATGATCC CCNTGAATCC 540
TCCCCCGGGA ACACTTANAT NCCCGTCNTC CNNGAANTTT CCCCTCTGGA TCNCCTTTAA 600 ACCCACNTGG ANATCCCCCN CNAGGAAGAA CTCCTTATGA ACNGCTTTTT AAAACATCGA 660
TNGGACCCNG TTTCCACTTC CNCATTNGCC MAATTGGGCG CCCTGGCCNT CCNCCCNTCC 72C
CCAA 724
(2) INFORMATION FOR SEQ ID NO: 19:
(1) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 797 base pairs (3) TYPE: nucleic acid
!C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(11) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U80
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:
GGCACGAGGG ACAAGCGATG GCAGCAGAAT TGTCTCCCAC GCTGAGCAAG AGCATCTTTG 60 AAGGAGCCGG TGGATCTTAC TCTACTTGGT CAGGTGCCGA TCTCCCCCTT CTTACTGATG 120
CAAAGCTCGG CGGAGGCAAG CTTGTCCTGA AACCACTGGG CTTGGCGTTG CCTCACTATT 180
CCGACTCATC GAAAGTCGGC TATGTTCTTG AAGGAAGAGC GGTGGTGGGG CTAACACTCT 240
ATGGAGAGAC CGAGCAGAGG ATACTGCTGC TTGAGAAGGG AGATGTGGTA GCGGTGGTCA 300
TGGGGAGCCT CACGTGGTGG TACAACGAGG AGGAGGACTC CGACTTCTCC ATCGCCTTCT 360 TAGGCGATAC CGCGACAGCT GTGCGACCGG GCGACATCGC CTACTTCTTC TTGGCAGGAT 420
CCCTAGGAAG TGCTCCATGG CTTTTCGACG GAAATTCCTC ANCAGGGCCT GCGGTTTAAG 480
GGGATGCGGA AGCTGANGAG CTCTTCGGAA GCCAACCTGG TACTCTAATC ATCACACNGC 540
AGCAAAAGCT GCCTGGCCTC AGANCATCCC GAGCTGACAG CNAAGGGATA TCNTAAACGC 600
TGANCGCGTA NCGGCATATA TCAATGTGAA AAGTGGTGGC TGTTCTGCCT CGGTGACCCT 660 TNATNAACTG GCNGCNCTGG GAAGATCAGG TTCTCCGTCN ACCTCCAAAA ATCCANCCTA 720
ACCCCGTGCC TTGCCGGGGT TCTTCNTTGA ATCNNCTGTT CANCTGAAAT TTTTCCCCAA 780
GGCCNTGGAA CAATTCT 797
(2) INFORMATION FOR SEQ ID NO: 20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 742 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U90
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20:
GGCACGAGAT GACTCTCTCG GCGGTAGCAT CGGATGCCGA TGATTCGGTC GCTTATACAT 60
TCGCTTCGCG ATACGTTCGC GAGGCTCTTC CCCGGTTCAG GATACCGGAG CAGTCGATCC 120
CCAAGGATGC GGCGTACCAG ATCATCAACG ACGAGCTGAT GCTCGACGGG AACCCGCGGT 180 TGAATCTGGC GTCGTTCGTG ACGACGTGGA TGGAGCCGGA GTGCGATCGC CTCATCATGG 240
CGGCCGTCAA CAAGAACTAC GTCGACATGG ACGAGTACCC CGTCACCACC GAGCTCCAGA 300
ATCGCTGCGT AAATATGATA GCCCACCTTT TCAATGCCCC AATTGGGGAA NACGAAACGG 360
CTGTTGGAGT TGGAACTGTG GGTTCCTCCA GAAGCAATCA TGCTTGCAGG ACTTGCATTC 420 AAGAGGAAAT GGCAGAACAA AAGAAAGGCA GAGGANAAGC CTTACGACAA ACCCAACNTT 480
GTTACCGGTG CAAATGTTCA GGTTTGCTGG GANAAATTTG CAAGGTATTT TGAAGTTTGA 540
ACTGAAAGAA ATTGAAGTTG AAAGAAGGAT ATTATGTTAA TGGATCCTGC CAAGGCAGTA 600
NAAATGGTTG ATGAAAAATA CATATGTTTT TGCTGCCATC TTTGGGTTCA ACTCTCCCTG 660 GGAAAATTTG AANATGTTAA CCTTCTAAAT GATCTCCTGA CNGAAAAAAA CCCAGAAACT 720
GGGTTGGACC CCCCNTACTT GT 742
(2) INFORMATION FOR SEQ ID NO: 21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 807 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U92
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:
GGCACGAGCT CTAGCCGATG ACTCTCTCGG CGGTAGCATC TGATGCCGAT GATTCGGTCG 60
CTTATACATT CGCTTCGCGA TACGTTCGCG AGGCTCTTCC CCGGTTCAGG ATACCGGAGC 120
AGTCGATCCC CAAGGATGCG GCGTACCANA TCATCAACGA CGAGCTGATG CTCGACGGGA 180
ACCCGCGGTT GAATCTGGCG TCGTTCGTGA CGACGTGGAT GGAGCCGGAG TGCGATCGCC 240 TCATCATGGC GGCCGTCAAC AAGAACTACG TCGACATGGA CGAGTACCCC GTCACCACCG 300
AGCTCCAGAA TCGCTGCGTA AATATGATAG CCCACCTTTT CAATGCCCCG ATTGGGGAAN 360
ACNAAACGGC TGTTGGAGTT GGAACTGTGG GGTCCTCAAA AGCAATCATG CTTGCAGGGC 420
TTGCATTCAA GAGGAAATGG CANAACAAAA GAAAGGCAGA GGANAAGCCT TACGACAAAC 480
CCAACATTGT TACCGGTGCA AATGTTCNGG TTTTGCTGGG AANAAATTTG CAAGGTATTT 540 TGAAATTTGA ACTGANAGAA NTGAANTTGA AAGAAGGATA TTATGTTTAT GGATCCCNGC 600
CCAAGGCAGT ANAAATGGTT GATGAAAATA CCAATTTNTG TTGCTTGCCC ATCTTTGGGT 660
TCCACTCTCN CTGGGANAAT TTTGAAAAAT GTTNAGCTTC TAAATGATCT CCNGGACCNA 720
AAAAANNCCC NNAANCTNGG TTGGGACCCC CCCNTACCTT TTCNATNCCT GCCANTTNGG 780
GGATTCNNTA NCGCCTTTCC TNCTATC 807 (2) INFORMATION FOR SEQ ID NO: 22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 770 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
( ) MOLECULE TYPE: cDNA
(vi ) IMMEDIATE SOURCE: (B) CLONE : U-U91
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:
GGCACGAGAG AAAAAATTGT AAAGTTTGAC TTTCATGGAC AGCCTGCGGA GCTCAAGCAT 60
GGTAGCCTTG TCATAGCAGC AATTACAAGC TGCACAAACA CATCAAATCC CAATGTTATG 120
CTTGGTGCTG GTCTTGTTGC AAAGAAGGCC TGTGAATTAG GTCTACAGGT TAAGCCTTGG 180
ATCAAAACCA GCCTTGCTCC AGGTTCTGGA GTTGTTACCA AGTATTTGCT TAAGAGTGGC 240 CTACAAGAAT ATTTGAATCA GCAAGGATTT AATATTGTTG GGTATGGCTG CACAACATGT 300
ATCGGAAACT CTGGGGATCT TGATGAGTCT GTGGCTGCTG CAATTTCCGA TAATGATATA 360
GTTGCTGCTG CTGTTCTATC TGGAAATAGG AATTTTGAGG GACGTGTGCA TCCATTGACA 420
CGAGCTAACT ATCTTGCTTC ACCTCCGCTT GTTGTAGCTT ATGCGCTTGC TGGAACAGTT 480
GACATTGATT TTGAAAAAGA ACCCAATTGG AACAGGGGAA GGATGGGAAA AAACATATAC 540 TTCCAGGATA TATGGCCGTC CACTGAANAA ATTGCACAGG TTGTGCAATC CANTGTTTTT 600
GCCTGAAATG TTCAAGAATA CCTATGAAGC AATCACCAAA GCACCCTATG TTGGAACCAA 660
CTGACTGTTC CCNGCCACAC TCTTTATTCA TGGGACCCAA TCCNCNTACA TCTTAACCAC 720
CNTATTTTAA GGANATGACC ATGGCCCNCC TGTCCNCATG GGGTGAAAAA 770
(2) INFORMATION FOR SEQ ID NO: 23: (l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 810 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U96
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:
GGCACGAGAG GGCCGTTCCT TTTTGTGCTC GCTTTGCTGC CTCCTCGTTC GTCTTTTCCG 60
CGAATTTCAC CGGCCATGAA CAGCCTTCGG AGCCTTCCGA CATCCTCCGC TCCGCCCTCG 120 CCGGTCCCGA GAGGACGTTC GAGTAGGCCA CCGACCTTGA ATCTGGGGGT GATACGACCA 180
CATCATCTTC GTGTAAATGT CGCCAGGAGT GCATGTTCCA AGTCTTCTGC AAGTCCTGAG 240
AAGACTGGTG CTGAAGTGCA AGATCAGAAG TCTGAGATAT ATAGCAGTGA TATGACAAAA 300
GCAATGGGTG CAGTGCTGAC ATATAGGCAT GAGCTTGGAA TGAATTATAA TTTCATCTAC 360
CCAGGATTGA TTGTTGGTTC TTGTCTACAG ACCCCATCAG ATGTTGATAA GCTTCCNAAA 420 GATTGGGGTT GAAGACCATC TTTTGCTTGC AGAAGGACTC GGATCTTTGA ATATTTTGGG 480
GTTGAAATTG GTGCAATTCC NTGAATATGC CATGCAATGT GGTGACATTG AACATCTTCN 540
TGCTGAAATC AGGGATTTTG ATGCATTTGA TTTGAAGATG ANACTTCCTG CNGTANTTAC 600
NAATTATACA AGGCTAATTA ATCCGAATGG TGGAATGACT TATTTTCNCT GTACANCCNG 660 GCTTGGAAAA CGCCTGCANT TGCNTTGGCA TACNTGTTTT TGGGTTNAAG GCTATGAACT 720
GAATGAAAGG GCCCCNCTAC TGCNAAATTA NCCAACTTGC TCCCCNAAAT TTGGTGCTAT 780
AAAAANTGCC ACCNTTGATA TGCTTATNGG 810
(2) INFORMATION FOR SEQ ID NO: 24: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 765 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U103
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:
GGCACGAGCT TGGCTGTAGT CTTCTTCTCA AGTAATCTTC GTCTTCCTGA TTTGCTTGGT 60
TGGTGGTCGG TTGCAGGGCG AAGGGATGGG GATGGGGAGG CCGAGCGGAG CTTGGTCGAC 120 CCCGTGGTTG GTGGTGGTTC TTGTGCATTG GCTCCTCTGG GCCACCGAGA GGAGGCGAGG 180
GGCGGTGGTG GAGGCCTCCC ATGTGGAGTT TGCATCCCTC CAATCTGTTC CTGCCTCCGT 240
CGTCGACAAC AGGCTGAGGA CTGGGTATCA CTTCCAGCCC CCGAGGAACT GGATCAACGA 300
TCCAAATGGA CCCATGTACT TCAATGGCGT CTACCACCTC TTCTACCAGT ACAACCCCAA 360
TGGCTCCGTG TGGGGTAACA TCGTGTGGGC CCACTCGGTG TCGACCGACC TCGTCAACTG 420 GATAGCACTC GACCCGGCCA TCCGCCCCAG CAAGCCATTC CACATCAACG GATGCTGGTC 480
CGGCTCCGCC ACCGTCCTCC CCGGCAACAG GCCTGCGATC TTCTACACCG GCATCNACCC 540
CCANCANAAA CAACTGCANA ACNTTNCNTN CCCAAGGATC TGTCCAACCT TACTCCCCNA 600
NTGGGTCAAC CCCACTNCAA CCCCGTGATC CCCCTGGCGA CGCATCAACN CCACCCCTTC 660
CNCNAACCCN AATACGGNTT GGCGCNGTCC CNGCACCCNC TGGAAACCCC TNGTNGGGAA 720 CNANTTGAAN CNGAAGGGGA AAGGCNTTCT TTNCCGAACA GGGAT 765
(2) INFORMATION FOR SEQ ID NO: 25:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 774 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U93
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25: GGCACGAGCG GAGAANGCAA TCTCGCTCTC TCTCTACTGC ATCGCGGCTC TCGTCCTCGA 60 TTACTTGAGA TATGGCAGGA AAGGGCGAGG GGCCGGCGAT CGGGATCGAT CTCGGCACCA 120
CGTACTCGTG CGTCGGGGTG TGGCAGCACG ACCGGGTGGA GATCATCGCC AACGATCAGG 180
GGAATCGTAC CACCCCCTCC TATGTCGCCT TCACCGACTC CGAGCGCCTT ATCGGCGACG 240
CTGCCAAGAA CCAGGTCGCC ATGAACCCCA TCAACACCGT CTTTGATGCA AAGCGTTTAA 300
TTGGTAGGCG ATTCAGTGAT TCATCTGTCC AGAGTGACAT CAAGCTCTGG CCTTTCAAGG 360
TCATTCCTGG TCCAGGTGAC AAGCCCATGA TTGTTGTCCA ATACAAAGGG GAGGATAAAC 420
AGTTTGCAGC TGAAGATATT TCTTCCATGG TTTTGATAAA GATGAAAGAA ATTGCTGAAC 480 CTACCTGGGG TCTGTTGTCA AGAATGCCGT CCTCACTGTT CCCGCTTACT TCAATGACTC 540
NCAACGCCCA GCCACAAAGG ATGCTGGTGT CATTGCTGGT CTCNATGTTA ATGANAATCA 600
TCAATGANCC CCCAGCACTG CTATTGCTTT ATGGCCTTGA CAAAANGCTA CTATTGTTGG 660
TGAAAAAAAT TTCCTCATCT TCCATCTCCG GTGGGTGGCC TTTTGANTTC NCCTTCCTNA 720
CCATCCAAGA AGGTTTCTTT NAAGTCAAGG CCCTGCTGGT GANACCCANC TTGG 774 (2) INFORMATION FOR SEQ ID NO: 26:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 786 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U125
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:
GGCACGAGCT AGTCTCGAGT TTTTTTTTTT TTTTTTTTAA AATTGAAATT AGATGCGATT 60 TCGAATAGCA AACATAGTTC AGGATAAACC AACAACATTG TACCGAATTC GATAAGCTTA 120
GGAAACTAAA TTGCGTAAAC GAAACACTTG CATGAAAGCC TATATAATAT AAGGCCGACT 180
AGGGACNAAA TAACTTAATC GACCTCCTCG ATCTTTGGAC CAGCGCCGCT GCCACCAGCA 240
GGAGGAGCAT CATCATCCAT ACCACCAGCC ATGTCAGCAC CTGCTCCCTG GTACATCTTG 300
GCGATGATGG GATTGCATAT GCTCTCCAGC TCCTTCATCT TGTCATCGAA CTCGTCGGCT 360 TCTGCCAACT GGTTGCCATC GAGCCATTGG ATGGCCTGCT CGATCGCATC CTCCGATCTT 420
CTTCTTGTCA GCAGCAGCAA GCTTGGAGGC AATCTTGTCG TCGTTAATGG TGTTCCTCAT 480
GTTGTNAGCA TANTTTTCCA GANCATTCTT CCGATTCCAC CTTCCTTTTT ATGCTCCTCC 540
ATCTTCCTGA CTTGTTACTT CTCCGCTTCC CTGCACCATT TTCTCAATCT CCTCCTTGCT 600
GACCTACCTT GTCCTTGGTG AAGGTGATCT TGTTCTCTGT CCAGTGGTCT NTCNTCNGCA 660 GAAANATTCC AGAATACCAT TGGGGTTCCA AATGTTNCAA AACCAAACCC GGTGGATTCT 720
TGAAGGAAAC CCCCCCCTAA GGGCCCCAGG GTGGGAAATC CCCCCAAAAA ACCCCCAAAT 780
TTTCCC 796
(2) INFORMATION FOR SEQ ID NO: 27: (l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 486 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
( i) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U105
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27:
GGCACGAGCG AGTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTCACAAG GNAACCATAG 60 ACATTACACC AAACATAGAT GTCAACAATA CATGCCAACG ATACCATTGC TTATTCCTTC 120
AGACCTCCGA TAGGACTTCC ACGCACACCA ACAGAGAGAA CTAAGACAGC AAAGCAGCGG 180
ACACATACAG CCCAAAAGGG TNNGTGACCC CCGCGGAGGN GGAGCNCCAG GTGAAGGGTG 240
GATTCCCTCT GAATGTTGTA TTCTGNCAGG GTGCGCCCGT CCTCNNTTTG NTTCCCNTCT 300
NANATCNCCC NCTGNTGATC TGGTGGNAAT CCCCCCCTTG TCCTGTGATT CTTGCTCTTG 360 ACNTTTTTCT CCCNTGTCNN ANCTCTCTNC CTNCTCTGGG TNTTGGGTCT TCCCCTGTTG 420
NANCNNTCTN TTNNCNTATC ATCTTGTNCT CCNNCCNCCN CNCCTNTCNT TTCCTCNNNN 480
GGTNNA 486
(2) INFORMATION FOR SEQ ID NO: 28:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 791 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(n) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U116
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:
GGCACGAGTG ACAATGTCAA GGCCAAGATT CAGGATAAGG AGGGCATTCC CCCCGACCAG 60
CAGAGGCTCA TCTTTGCCGG CAAGCAGCTC GAGGATGGCC GCACCTTGGC TGATTACAAC 120
ATCCAGAAGG AGTCCACCCT CCACCTTGTC CTCCGCCTCC GTGGTGGCAT GCAAATCTTC 180 GTCAAGACTT TGACTGGGAA GACCATCACC CTTGAGGTGG ANAGCTCGGA CACCATCGAC 240
AATGTAAAGG CCAAGATTCA GGACAAGGAG GGTNTTCCCC CGGACCAGCA AAGGCTCATC 300
TTTGCCGGCA AGCAGCTTGA GGATGGCCGC ACCCTGGCAG ATTACAACAT TCAGAAGGAG 360
TCTACCCTTC ACCTTGTGCT GANACTTAGG GGTGGCATGC ANATCTTTGT TAAGACNCTC 420
NCAGGGGAAG ACCATTACCT TGGAGGTGGA NANCTCNGAC ACGATTGATA ATGTCNAGGC 480 AAAGATCCAN GACAAGGANG GGATTCCACC GGATCAGCAN ANGCTGATCT TTGCTGGGAA 540 ACACTGGANG AAGGGCGCAC CCTGGCAGAT TNCNACATTC CNAAAGAATC CACCTTCACC 600
TGGTGCTCCC CCTCCGCCGG GGTCCCTAAC CCTTTTGGGC TGTATGTNTC CCCTGCCTTT 660
GCTGTCTTAA TTCNCTCTGT TGGTGTTCGT TGGAAATTCC CTAATCCNGA AGGTTCTTNA 720
AAAGGAAATN AANCCNNTTG GGTTTTCCCC TTTGGCCTTT TTTTTGTTTG AAAATCCTNT 780
GTTTTTGGTG T 791 (2) INFORMATION FOR SEQ ID NO: 29:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 814 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U120
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:
GGCACNANCC CGCTTCGGTA GCCATTATTG GTGCCCGTAG AGCGAGTGCT GAGATGGCTT 60
TGAGGGCCTT CTTCCCCCTC TGCATCGCTC TCGTGGTGAC GCTCTCACCT CTGTGTGATG 120
CCACTTCGCC TTACTACACC ATCACACCGC CCACCCCCGT GGCCAAGCCG CCTTCAGTTG 180 AACCACCACC CTACCACGGC CCTCCGACNA CCCACCCTAA GCCACCGAGT CATGGTGGCC 240
AACCTCCGTC CCACCATCAC CCAACACCAA TCTACGGTGC ACCCCCTCCG CAACACCACC 300
ACCACCACCA ACACCACCAC CAACCTGCAC CACCAACTCA CGCANAACAC CCTCCGTACT 360
ACCACATGCC TTCCCCGCCG CCGCATGGCC AGCACCCGTC ACCACCGTCA CATGATTATC 420
CCGTACCTCC TGCTCACAAG CCCCGAACTC CGCCGCCGGT TTACAAGTCT CCACCACCGA Θ0 NCCACCGTCC TTACCCTCCA TCGACGCCAC CCCACCATCC GAAGCACCCG CCTTCTCAGC 540
CGAAGCCGTC NTACAAGGCC CGCCACCATN CAAGAACATC CCTGANCACT CTCCACCGCC 600
GCGTCACTAT CATTCTCCGT CTTCACCACC ACCACCACCA CCATACAAAA TAATCTCGTT 660
TTGCCATCTT CTCCCGTTTG AAAAAAAANN CAANTGTCCT TTTTATTTAA CCNAATCCAN 720
GGGGTTTTTN TTAANATTTN AANAAANCNA ANTNTTTTCC CCCCCNTNTT GGTTNATGGC 780 CTATGGGTTT GTTTCTCTTC TTGGCTTTTC CCGG 814
(2) INFORMATION FOR SEQ ID NO: 30:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 742 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown Ui) MOLECULE TYPE: cDNA
(vu) IMMEDIATE SOURCE: (B) CLONE: U-U126 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30: GGCACGAGGT TGTGCCTAAC AGAGAGAGAG AGAGACAGAC CGATAGCCTC CTCATTCACT 60
ATGGCGATCC GATCGCCAGC TTCGCTGCTG TTATTTGCGT TTCTGATGCT TGCGCTCACA 120
GGAAGACTGC AGGCCGGGCG CAGCTCATGC ATTGGCGTCT ACTGGGGACA AAACACAGAC 180 GAGGGAAGCT TAGCAGATGC TTGTGCCACA GGCAACTACG AATACGTGAA CATCGCCACC 240
CTTTTCAAGT TTGGCATGGG CCAAACTCCA NANATCAACC TCGCCGGCCA CTGTGACCCT 300
CGGAACAACG GCTGCGCGCG CTTAAGCAGC GAAATCCAGT CCTGCCAGGA GCGTGGAGTC 360
AAGGTGATGC TCTCCATCGG AGGTGGCGGG TCTTATGGCC TGAGTTCCAC CGAANACGCC 420
AAGGACGTGG CGTCATACCT CTGGCACAGT TTCTTGGGTG GTTCTGCTGC TCGCTACTCT 480 CNACCCCTCG GGGATGCGGT TCTGGATGGC ATANACTTCA ACATCCCCGG AGGGAGCACA 540
GAACACTATG ATGAACTTGC CGCTTTCCTC CNAGGGCTAC AACGAACAGG AAGCCGGAAC 600
CAAAAAAGTT TCACTTGAAT TGCTGCTCCC NCANTGTCCT TTCCCCGATT ACTGGCTTTG 660
GCAACCCACT CCAAAACAAA TNTCTTCCNA CTTCCNTGTT GGGTTGCNAA NTTCCNTTCC 720
CAANCAAACC CCTTTCNTTT GC 742 (2) INFORMATION FOR SEQ ID NO: 31:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 794 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U129
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31:
GGCACGAGGG CGTGGCGGAG ATGGGGAGCT GGCGGGCTCT GTTGCAGCGG CGGCTGCTGT 60 TGCTCTCTGC TTTGGCGGTG GCTGTTCGTG TGAAGGCACT CAGCANANAC NATTTCCCCG 120
CCGGCTTCAT TTTTGGCGCA GGCACCTCCG CTTATCAGGT ANAAGGTGCA GCTGCAGAGG 180
GGGGAANAAC ACCCAGCATT TGGGACACGT TTACGCATGC AGGGAGAACT TTCGACCAGA 240
GCACCGGAGA CGTAGCAGCT GATCAGTATC ACAAGTACAA GGAAGATGTG AANCTGATGC 300
ATGANATGGG CTTCGATGCT TACAGATTCT CCATCTCCTG GTCCANAGTT ATCCCCAATG 360 GTCGAGGGCC TGTGAATCCA CAAGGCTTGC GGTACTACAA CAACCTGATC GATGAGCTCN 420
AAAGATATGG AATCCGAGCC TCATGTCACT CTTTACCACT TCGACCTTCC GCAAGCACTG 480
GAANACGAAT NCGCCGGGCA GCTGAACCCA AAGATCGTAN ANGACTTCAC CGCTTACGCC 540
AACGTTTTGC TTCANCNAAT TTGGGGATCN AATCTAGCAC TGGATCNCCC ATCAATGAAC 600
CAACATANAT CCCGTCCTCC GCCACNAATT CCGCATCTTC NCCCCTGGCC CCTGCTCTTT 660 ATCCCTTNCG CCTCCAACTG CNCCAAGGGC NACTCCCCCC CATTTTNAAN CCCATNNNNT 720 CCNCCCCCCC CAATTAACCC NTTCTTGCTC TTCCCCCTTG CCTTCANCCA ACCCGCCCNT 780 TTTCCNAAAA AANA 794 (2) INFORMATION FOR SEQ ID NO: 32:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 799 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U130
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:
GGCACGAGCC GGCCTCAGGC ATATGGTGCG GTCGCTACAC GTGTTCAGGC AAGGCCGGGA 60 GCCGGCCAGG ATCATCAGGG AAGCACTGTC GAAGGCGCTG GTGAAGTACT ACCCCTTCGC 120
GGGGCGGTTC GTGGACNATC CCGAGGGCGG CGGCGAGGTT CGTGTCGCTT GCACTGGCGA 180
GGGCGCTTGG TTCGTCNAGG CCAAGGCGGA CTGCAGCTTG GAGGACGTGA AGTACCTCGA 240
TCTCCCGCTC ATGATCCCTG AGGACGCGCT CCTGCCCAAG CCCTGCCCGG GACTGAACCC 300
CCTCGACCTC CCTCTCATGC TGCAGGTGAC ANANTTCGTG GGCGGCGGAT TCGTGGTCGG 360 CCTCATCTCC GTCCATACCA TCGCCGACGG CCTCGGCGTC NTCCANTTCA TCAACGCCGT 420
CGCCCGAAAT CGCCCGTGGC CTGCCNAANC CCACCGTGGA NCCTGCATGG TCCCGGGANG 480
TCNTACCCAA CCCACCTAAG CTGCCTCCCG GTGGCCCGCC CGTGTTTCCC CTCCTTCAAN 540
CTGCTCCACG CCACCGTCGA ACTATCCCCT GACCACATCC ATCACGTCAA GTCCCCACAC 600
TTTGGANCTC ACCCGGCCAA CGCTGCTCTA CCTTCCACNT CCCCATCCCC AACCTGTTTG 660 NAATCCCGCA CGCCCCGCCN TCNACCTTGG AACCCAAGGC NTTTCCAANC TTTGCAACCC 720
TTTTTTCCTT TCCTTCCCCC CCCAAAANAA CTTCCCCCCA CCCTTGTTTT TGCNCCCAAG 780
TTTTTTCCTT CCTTGCCCC 799
(2) INFORMATION FOR SEQ ID NO: 33:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 759 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
Ul) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (3) CLONE: U-U43
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33: GGAGGTTGTT GTTCATGTCC CTACTTCGGG GTCTCCTGTA CAGATTGAAT TTCAAGTAAC 60 TAATAGCAGT GGCTACTTGG TGCTTCATTG GGGTGCAATT CATAATAGAA GGAATAACTG 120 GTCACTTCCT TCTCGTCATC CTGATGGAAC AAAAGTATAC AAAAACCGAG CTCTCAGGAC 180
ACCTTTTAAA AAATCTGGTT CCACATCATC AGTAAAAATG GAGATTGATG ATCCTGAAAT 240
ACAAGCAGTT GAGTTTCTCA TATTTGACGA GTCAGAAAAC AAATGGTTTA AACATAATGG 300
TCAGAATTTC CATGTCCAGT TATTAAAACA GGGCTATCAA AATCAAAATG TTTTGGCCTC 360 TGTAAATCCA AATGTTTCAC TGCCANAAAG AACTTGTGCA GATTCAAGCT TATCTTAGGT 420
GGGANANAAA GGGTAGGCAA ACATATACAC CTGATCAAGA AAAGGATGAG TNTGAAGCAG 480
CTANAACTGA GTTTGCTANA AAAACTAAGT NNAAGTACTT CTGTANANGA NCTCCGATCT 540
AAATTGACAA AAAACNCCAC TGCTGGAGCG GACAAATCCG TCAAAGTTGA AACTATCAAA 600
AGTGGGATCC GGATGATCTT NTGCAAGTTA CANGCATACT TCCNATNGGA AAAACCGGGA 660 AACCATNTTA TCCCCCNGAA AAACACTNAT GGAATTGAAG AAACCAGGGA AANACTGCCC 720
ATGAATTAAA TAAANGAACN CNCCTNCAAA CTNAGGGAA 759
(2) INFORMATION FOR SEQ ID NO: 34:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 785 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U70
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34:
GGCACGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 60
AGAGTAATCG ATTTAGCGTG CAAAGTACTG CAGCAGAAGC CATGGCATCC ANGGCCTCAG 12C
CNTCGGCTGC CCTCTTCCTT GCTCTCAACC TTGTCTTCTT TGCACTCACC AGTGCTTGTG 180
GCACCACTTG CACCCCCGCC ACTAAGCCCC CACCGAGCTC TTCCCATGGC AAATGCCCCG 240 TCGACACCCT CAAGCTGGCC TCCTGCGCCA ACGTGCTCAA TGGCCTGATC NNGGTCGGCG 300
TCGGCAAGCT CCCGAAACAG CCGTGCGAGT GCTGCNCTCT GCTCGATGGC CTCGTCGACC 360
TCGAGGCCGC CGTGTGCCTT TGCNCTGCTA TCANGGCCNA TGTCCTTGGC ATTCATCTCA 420
ACCTCCCCAT CNNCTTCAGC CNTCTCCTCA ACTACTGCGG TNAGAAGGCT CCCACCGGTT 480
TCCAGTGCCC TTGAGCTCCC CCTGCGGATC ACATCNTCGA TCCTCTTTGC CCGTGGTGAA 540 TTTTMTTGAG CATTCCTTTG TGTCTTTACT TCCCTTTGTT TTTGGCTTTT TGATGACGAA 600
AGTGTTGAGT GTTGATATGA AGGGCCANTG GCGCTTTCNT GTTAATGTTA CCTGTCNTTC 660
CCTTTTTATT TCCACNNCTT GTTGANTTTC CCTCTCGGGT TTGTTAATGA AGATCNATGC 72C
ATTCAATTNT TAATATTTCT GCTGCCTTCC AANAAAAAAT ANNANTMCCT CCNNGAANTC 78C
TTCCT 785 (2) INFORMATION FOR SEQ ID MO: 35: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 784 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U16
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35:
CGGCACGAGG AAANGACNAG CGATGGCAGC AGAATTGTCT CCCACGCTGA GCAAGAGCAT 60
CTTTGAAGGA GCCGGTGGAT CTTACTCTAC TTGGTCAGGT GCCGATCTCC CCCTTCTTAC 120
TGATGCAAAG CTCGGCGGAG GCAAGCTTGT CCTGAAACCA CTGGGCTTGG CGTTGCCTCA 180
CTATTCCGAC TCATCGAAAG TCGGCTATGT TCTTGAAGGA AGAGCGGTGG TGGGGCTAAC 240 ACTCTATGGA GAGACCGAGC AGAGGATACT GCTGCTTGAG AAGGGAGATG TGGTAGCGGT 300
GGTCATGGGG AGCCTCACGT GGTGGTACAA CGAGGAGGAG GACTCCGACT TCTCCATCGC 360
CTTCTTAGGC GATACCGCGA CAGCTGTGCG ACCGGGCGAC ATCGCCTACT TCTTCTTGGC 420
AGGATCCCTA GGAGTGCTCC ATGGCTTTTC GACGGAATTC CTCANCAGGG CCTGCGGTAT 480
AAGGGATGCG GAANCTGAAG AGCTCTTCGG AAGCCAACCT GGTACTCTAA TCATCNCACT 540 GCANCAAAAG CTGCCTGGCC TCANAAGCAT CCCGAGCTTG ACNGCGAAGG GATAGTCNTN 600
AACNCTNANC GCGTTNCGGC ATATATCAAT GTNAANAGTG GTGGCTGTGC TGCGTCCGTG 660
ACACTTNATG AACTGGCCGC GCTGGGAAGA TCTGTTCTCC GTCNAACTCA CCANANTCNA 720
ACCTAACCCC NTNCNCTTCC NGGGTTCTCC TTNATGCACT NTGCNCCTAT TTNTTTCCCA 780
ANGC 784 (2) INFORMATION FOR SEQ ID NO: 36:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 817 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U30
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36:
CGGCACGAGA TATGGAGGTA TGATCGGGAG CTACTGGGAG CTCCGCTTTG GTGAACAACA 60 CGACGGCAAG AAGGGCTATG GATTCAAGGA GTGAAGGCCA TGGTACCGCA NAGGCGGGTC 120
TTCCGGGCGT GCACCGAATT TTGCATCGGA TGAAAACCTT GGTCATCAGC A ATGGGGGC 180
TGGGTTCCAC CAAGGGAAAA GTTCGAATGC AAGTACCAGT GAGTCCCATG AGAGGGACTT 240
GATCATGCAG AGGTATGATC GAAGCAGCTG GAGANTTGGA CTGCTCCANA GCTCATATTC 300 GCTTAAGGGA GCCCGACAAG TCAGAGGACA AGGTCGAGTA AGCGAACGTT GCTACCCAAA 360
ATCAAGCATC AGTTANAATG GAGGTGGACT CANAGGAGTG CCACGGAAGA CATCTCTACT 420
GATTGTGAAG GAAAGGGATA CAGAGGCGAA NCGACGGATA NTANGGCCAT GGGCATGGCA 480
GCGCCATGGT ACCGCANANG CGGGACTTCC GTGCAAGTCA TTGATCCCTT GCTCTCACGG 540 AGGGANANCG CTTGGTCCTG AAAGGGGCCN AAGAAGTGGA CATGCANANG CAATCTCCAA 600
TTACCGAAAC ANGGCTGAAG GGCANAAGCC NANAAACTTC CTNAGAACGG TGTCAACAAT 660
TTCTCATCNA NATANCCGTA ATTAANGACT TCCGGTCATG CNANAATNCC CNACCNANGA 720
ACCAANCAGG CNTACTTGTG CTGTNCTTTG CTACCCNNTG AATNGCGCCN GGTTNATNGA 780
AAAAACGTCC TTCCCCAAGC AACCCTCTNT CAAAAAN 817 (2) INFORMATION FOR SEQ ID NO: 37:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 837 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U40
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37: ττττττχτττ TTTTTTTTGG GTATCAGGCA AAGATGGATA CTTCCAGGTT TGCTATTTAA 60 GAACCATAGT CAGTCTTATG AGGAGAGACC ATACGTAATA CAATCTTTAA GATGACAAAA 120
TCCATTCCTA CCAATACAAT GACCAAGCGA AAGGTACAAG GATAACGGGC AAAAGACTAA 180
ATTGCAACTA TATAAAATTC TGCTGCATTG TAGTAAATTC AACTGTGACG ACACGCCACC 240
AGGATGAAGT CTATCTATAA ACCCACTTAT TGCTAGATGG ACGGTGTAAC AGAGCTAAAA 300
CAGCAAAGTA GAATAAAAGA AACTGACAAC GCTTCCGCAT GCGCTCCACA TATGGAGCCA 360 GGCGGAGGTT GGGCAATTGG AGGAAATGAC NTTTCTGACG GTGTAACATA AAANAAATTT 420
GTAACACCGT CAAGTACGAT TTCCCATCCT TCGGGTTGTG GCGTTCTGCT ACTTGGCATT 480
ACGTTAACAT CGAGTGCCGA CTTGGCAAGA CAGGACCCCA GCATCAGAAG TCAGTGTGTC - 540
AACCAAGGAA GAAGGCTTGG CCCCAAAGCT GGAGGCCCGT GCGTAACTGG CTGAACCCCT 600
GCACCGGAAN GGGTGAAAAA AGCACCATTC AGAACAGGTC NCCTCCGACC TCCCAATTCC 660 AGTTCTTCCA CCNTGCTTTT GATCTGTGTC CACCTTTTTG TTACCTATTC CATCGAAGCN 720
TANGGATTTT TAAAAATCCC GAAGGAAAGG AANTTCCNAT AATTGGTGCN CCAAANAAGG 780
AAGATGGACN GAACTGTTGT TGGTTGCTCC TGTTCCTCCT TTGCAATGGA TTGGTCG 83"
!2) INFORMATION FOR SEQ ID NO: 38:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 799 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U108
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38:
GGCACGAGGC AGCTCAATCG ACCGACTCCT CCTCCTCCTC CTCCTCCTCC TCTTGTTCTT 60 CTCATTCTAA TATAATTATC GCTTTTGGTG TGTACATCTT CTATAATTTG AGAGGATGGA 120
GGCAGGATTG ATGGCGAAAC AAGCAGCAGC GGTTGCGAAG CCGAACGCGT TCCCCGCCCG 180
GAGCCTAGGG TTCGGGAGCT CGGTCAGGGG CGGATCCGGG ACCAGCAGGA TCGGGTTCGA 240
GGCGCCCGCG AGCGTGGCAT GGAGGAAGCG GTCGATTCAG GTGGCCCGCC AAGGAGCCAT 300
TCGGTCGGAG GTGGTCGTGG AANANAAAGC ATCNCCGCCC AAAAAGGATA AGGCCGGTCC 360 GGGTCGGCTC TACGTGGGTC TGCCCTTGGA CGTGGTCTCC GACGGCAACG TCNTCAACCA 420
CGGCAAGGCC ATCGCCGCCG GTCTCCGCGC CCTCCGCCCT CCTCGGCGTT GATGGANTCN 480
AACTCCCCCA TCTCCTGGGG CGTTGGCGAT GGACTCCGGC GAATGGTCCT CCTACCTCCC 540
CCGTCNCCGC CATGGCGCGC GACNCCCGGC CTTCGCCTCC GCGTCTCCCT CCACCTCCAC 600
TGCCANCGCC GCCCTCGCCT CCCCCTCCCC NAATCGGTCG AATCCNCCGC CGCTAACAAA 660 CCCCGAAATC CTATTCNCTG AACGCNCCGG CCGCCNCCNG TTGCCCAAAN TNGCCTTNTT 720
CCTTTCCNCT TTGTTTNAAA AAAAACTCCC CNTTGTTCCT TCCAANCGGG NAGGAACCCC 780
CNAATGGAAA CCTTNCAAA 799
(2) INFORMATION FOR SEQ ID NO: 39:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 786 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-U128
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39:
GGCACGAGGA AACGTCTCTC CCTCTCTCCA CTTCTCACAC CCCCGCCCCC GACTCAGCGA 60
ATCGACATTC TTGGCGTGTC AAGGAAACCT TTCTTTTATT TGCGAAGGAA CATGAGCAAT 120
ACTGTTGGAC ACACCTTACC CCAGCAAGCT CTTTACCGGC CTTCTATCAA AGAAAATCAA 180 AGTAAAGCTC ATCAAGGAGT TTCTGCAAAC TTCTTGTGTG GAGTTCCATT AGGATCTAAG 240
GTGGAAAATG CAGTGTCATA CAGCTCTAGG TCACTGCTTT CAACCGGATC CCTGGGAAAG 300
AAACTCATCA AAGGAATC . ACCAAAGCAA AATCCAAGCA TAGTCACTAT GACCCCTCGT 360
GCTGTGTTGG CTGCANATCC TGCTTCAGAG CTAAGGAGAA AATTCAAGCT TGACACAAAT 420 TCTGAATTGG AGGTTGTTGT TCATGTCCCT ACTTCGGGGT CTCCTGTNCA GATTGAATTT 480
CAAGTNACTA ACAGCAGTGG CTACTTGGTG CTTCNTTGGG GTGCAATTCA TAATANAAGG 540
AATAACTGGT CACTTCCTTC TCGTCATCCT GATGGAACAA AAGTTTNCCN AAACCCGAAC 600
TCTCANGANA CCTTTTAAAA AATCTGGTTC CCCATCNTCC GTNAAAATGG AGATTGATGA 660 TCCTGAAATA CCAGCAGTNG AATTTCTCCT TANTTTTGNA ACAAATNCTC AAAAAAACCN 720
AAATTGGGTT TTTAAAACNA TTAATGGGTC CCNAAATTTC CNTGTTCCNN TTTTTTTANA 780
AACAAG 786
(2) INFORMATION FOR SEQ ID NO: 40:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 804 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D4
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 40:
GGCACGAGAG ATAGCTGAAG TGACGTGAGG TGACTTCGAT TAGATCGAGA GAAATTTCTT 60
TGTGATTTTT GTGGACAATT CTCAATGGCT GCTGTAACAA ANTCACAATT CATCTCGAAA 120
GGCTCATGCG CTGGCTATGG GGGTGTCGTC GACTCGGAGC CAAGGACCTT CCTAAACAGA 180 AGGGTTCTAC ACTTGAGGAA CCAAACCACC GCTTACGAAG GATTGAGATC TCGAAATGTG 240
GTTGATTTGA TTCAGATGCC GTCTAATGCC AAGGTGATTT CAAGGAAAAC TGTGAGGGGA 300
ACTCAGAATC CTAGTCGTAN ACCTTGGGCT GTTGTCATTT GTGGAAAAGG GATGAACATA 360
GTCTTTGTCG GCGCTGANAT GGCTCCCTGG AGTAAAACCG GANGGACTCG GTGATGTTCT 420
TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACAGA NTGATGACTA TAGCTCCACC 480 CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG GCTGAATTAA AAGTTGGAAA 540
TGAAATTGAA ANAATCCGCT TTCTTCCACT GCTATAAAAA AAGAATTGAC AGGGTTTTCA 600
TTGATCACCT TTGTTTCTTG AAAANGTGTT GGGAAAAACT GGAAGAATGA AATTTTGGTC 660
CTNTCCCCGG AACTGATTTT NAAAAACANC CCCTAAANTT TANCCTTTTN TNCCCNGCCN 720
CTTTTGGAAA CTCCCNGGAT NCTGGTTTTT TNCCACCCCC NAAAANTTTC CTNGAACCTN 780 TGGGGGAAAN ττ--"-TTTTTT TTTT 804
(2) INFORMATION FOR SEQ ID NO: 41:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 826 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D10
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41: GGCACGAGAG ATAGCTGAAG TGACGTGAGT GGACAATTCT CAATGGCTGC TGTAACAAAG 60
TCACAATTCA TCTCGAAAGG CTCATGCGCT GGCTATGGGG GTGTCGTCGA CTCTGAGCCA 120
AGGACCTTCC TAAACAGAAG GGTTCTACAC TTGAGGAACC AAACCACCGC TTACGAAGGA 180 TTGAGATCTC GAAATGTGGT TGATTTGATT CAGATGCCGT CTAATGCCAA GGTGATTTCG 240
AGGAAAACTG TGAGGGGAAC TCAGAATCCT AGTCGTANAC CTTGGGCTGT TGTCATTTGT 300
GGAAAAGGGA TGAACATAGT CTTTGTCGGC GCTGANATGG CTCCCTGGAG TAAAACCGGA 360
GGACTCGGTG ATGTTCTTGG AGGACTGCCA CCGGCCATGG CTGCAAATGG ACACAGAAGT 420
GATGACTATA GCTCCACGCT ATGATCAGTA CAAGGATGGG TGGGATACAA ATGTCCTGGC 480 TGANTTAAAA GTTGGAAATG AAATTGAAAG AATCCGCTTC TTCCACTGCT ATNAAANAAG 540
AATTGACAGG GTTTTCATTG ATCATCCTTT GTTTTCTTGA AAANGTGTTG GGAAAAACTG 600
GANGAATGAT ATNTGGTCCT GTCCCNGGAA CGGATTATTN AAAACCACCC NCTTAANATT 660
TACCCTTTTG TGCCCANGCA NCNTTTGGAA NCTCCCAAGG ATTCTGGATN TTTAACNACN 720
NCCNAATACT TTTCCTGGAA CCATTTNGGG GANAANNTTG TTNTTTGTTG CNAATTAATT 780 GGCNCCCTGG TCCTCCTNCC TGCTACTTAA AAATTTTNTT TCNTNT 826
(2) INFORMATION FOR SEQ ID NO: 42:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D13
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42: GGCACGAGCT GAGGGAGTGA GGGAAAGAGA TAGCTGAAGT GACGTGAGGT GACTTCGATT 60
AGATCGAGAG AAATTTCTTT GTGATTTTTG TGGACAATTC TCAATGGCTG CTGTAACAAA 120
GTCACAATTC ATCTCGAAAG GCTCATGCGC TGGCTATGGG GGTGTCGTCG ACTCGGAGCC 180
AAGGACCTTC CTAAACAGAA GGGTTCTACA CTTGAGGAAC CAAACCACCG CTTACGAAGG 240
ATTGAGATCT CGAAATGTGG TTGATTTGAT TCAGATGCCG TCT ATGCCA AGGTGATTTC 300 AAGGGAAAAC TGTGAGGGGA ACTCAGAATC CTAGTCGTAG ACCTTGGGCT GTTGTCATTT 360
GTGGAAAAGG GATGAACATA GTCTTTGTCG GCGCTGANAT GGCTCCCTGG GAGTAAAACC 420
GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGGCC ATGGCTGCAA ATGGACACAG 480
AAGTGATGAC TATAGCTCCA CGCTATGATC AGTACAAGGA TGGGTGGGAT NCAAATGTCC 540 TGGCTGAATT AAAAGTTGGG AAATGAANTT GAAAGAATCC GCTTCTTCCA CTGCTATAAA 600
ANAAGAATTG ACAGGGTTTT CATTGATCAC CTTTGTTCTT GAAAAGGTGT GGGGAAAAAC 660
TGGAAGAATG ATATTTGGTC CTGTCCCNGG ACTGATTTNA AAACACCACC TAAAATTACC 720
TTTTGTNCCN GCNNCTTTGG AACCNCCCNG GATCTNGATN TTANCACCCC CAANTCTTAT 780 CCTGGAACTT TTGGGGNAAA NTTTGTNTTT GTT 813
(2) INFORMATION FOR SEQ ID NO: 43:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 805 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
Cvii) IMMEDIATE SOURCE: (B) CLONE: U-D66
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 43: GGCACGAGAA GAGATAGCTG AAGTGACGTG AGTGGACAAT TCTCAATGGC TGCTGTAACA 60
AAGTCACAAT TCATCTCGAA AGGCTCATGC GCTGGCTATG GGGGTGTCGT CGACTCGGAG 120
CCAAGGACCT TCCTAAACAG AAGGGTTCTA CACTTGAGGA ACCANACCAC CGCTTACGAA 180
GGATTGANAT CTCGAAATGT GGTTGATTTG ATTCAGATGC CGTCTAATGC CAAGGTGATT 240
TCAAGGAAAA CTGTGAGGGG AACTCACAAT CCTAGTCGTA NACCTTGGGC TGTTGTCATT 300 TGTGGAAAAG GGATGAACAT AGTCTTTGTC GGCGCTGAGA TGGCTCCCTG GANTAAAACC 360
GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACACA 420
GTGATGACTA TAGCTCCACG CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG 480
GCTGANTTAA AANTTGGAAA TGAAATTGAA AAGANTCCGC TTCTTCCACT GCTATAAAAN 540
AAGAATTGAC AGGGTTTTCA TTGATCATCC TTTGTTTCTT GAAAAAGTGT GGGGAAAAAC 600 TGGGAAGAAT GATATNTGGT CCTGTCCCNG GAACTGATTA TAAAACACCA GCTAANATTA 660
CCTTTTGTTG CCAGCAACTT TGGAACCCCN AGGATTCTGG ATNTTACAAC ACNAATACTA 720
TTCTGGAACA TTTGGGGAAA AA.TTGTTTTT GTTNCCAATG ANTGGCNCAC TGGTCCCNTC 780
CCTNCCTACT TAAAAANTTT TTTCC 805
(2) INFORMATION FOR SEQ ID NO: 44: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 790 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-Dlll (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44:
GGCACGAGAA GAGATAGCTG AAGTGACGTG AGTGGACAAT TCTCAATGGC TGCTGTAACA 60
AAGTCACAAT TCATCTCGAA AGGCTCATGC GCTGGCTATG GGGGTGTCGT CGACTCTGAG 120
CCAAGGACCT TCCTAAACAG AAGGGTTCTA CACTTGAGGA ACCAAACCAC CGCTTACGAA 180
GGATTGAGAT CTCGAAATGT GGTTGATTTG ATTCAGATGC CGTCTAATGC CAAGGTGATT 240
TCGAGGAAAA CTGTGAGGGG AACTCAGAAT CCTAGTCGTA GACCTTGGGC TGTTGTCATT 300 TGTGGAAAAG GGATGAACAT AGTCTTTGTC GGCGCTGANA TGGCTCCCTG GAGTAAAACC 360
GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACAGA 420
GTGATGACTA TAGCTCCACG CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG 480
GCTGAGTTAA AAGTTGGAAA TGAAATTGAA AGANTCCGCT TCTTCCACTG CTATAAAAGA 540
AGANTTGACA GGGTTTTCAT TGATCATCCT TTGTTTCTTG ANAAGGTGTG GGGAAAAACT 600 GGAAGAATGA TATATGGTCC CTGTCNCAGG AACNGATTAT GAANACAACC AGCTAAGATT 660
AACCTTTTGT TGCCAGCACT TTGGGAACCN CAAGGATCCT GGATCTTAAC AACNCAANTC 720
TATTCTGGAC CATATGGGGA CAAATTGTTT TGTTGCAAAT GATGGCACCT GGTCCNNTCC 780
ATGCTACTTA 790
(2) INFORMATION FOR SEQ ID NO: 45: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 813 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D112
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45: τττττ,τττττ TTTTTTTTTG GAGCATAATA GCAATTTATT CAGGAGGATC AAACTCAAAC 60
ACTATAACAT AGACAGTAGT CACTTTCAGG GAGTGGCCAC ATTTTCCTTT GCAAGAGGAG 120 CTATTTCATC AGCATCGATG CCGGCTTCAC TACCAGCAGC TCCTAAGCTT AGGANAAATT 180
GCTCCCACTT CTTAGCAGGT CCCTTCCAGG AGAGGTCTTG AGTCATGCAG TTCTGTATCA 240
TCTCAGCAAA TGCAGGTGTG CCGTAAACTT TAAGGGCCCT TTTCACTGTC TGAATGACTT 300
TTTGTACATC ATCTTTATCG ACAACATCAC AATCAGGATT GAAGGGACCC ATATGAAACC 360
CTGTGATGCC TTCTATGACA GTGTCAGCAA GTCCACCAGT TGTTGCGCAC ATGGGGGGAA 420 TTCCATATCG CATGCCCTGA AAGCTGGATA AGGCCACAAG GTTCGAATCT GCTGGTAAGA 480
NCAAGAAGAT CTGCTCCAGC CATGATTTCA TGANCCAAAG GTGCATTAAA CTTCATATGT 540
GCTCTCACTT TATTGGGAAA CATATCTTCA AGTTGTGCAA GCTGACGCTC CAACANCTTC 600
TCCCANTACC AAGCACTATC ACTTGAACAT TTTCATCAAT GAATTCAGGA ATAACTGCNG 660 CGAAAATATC CGAACCTTTC TGCTCTCTAA TCTCCCTATA AAGGNTATAA CAGGGATTTC 720 CNGTCAACAG GTTAACCAAA TTCAACTTGT TAGGNTTCCC TTAATCCAAA GGTTTGCACC 780
CNNCCCATTT GTTGCTCNTA ATTGANAAAA ATT 813
(2) INFORMATION FOR SEQ ID NO: 46: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 808 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D39
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46:
GGCACGAGCG AGTTTTTTTT TTTTTTTTTT GCTGTGATGA TATTATGCAT CTTATTAGTT 60
GGTTGCACCA CTTCTATTAC ATCACTCAAC AGTATTAGCC CAAGANAAGG CTAATACCTT 120 CNCATCACTC TTGACATCTT ATTTGTATTG TAGGANACTA GTATATATAT CTATATGCGT 180
GGTTGCCTGC ACNAGGGCGG CTTATTAGTG GCGTCAGTGA TCTCAAGGGC AGAANACAAC 240
CCTGTANTTG GTGCCGCCGG GGCAGGTGAA GGTGCTCGTC TGATCGTCCT TGGGATANCT 300
GTAGGCGTCG GGGCAGTTTC TCTTGAANAA CCGGGAGTAT TCGGTCGGGC TGCAGCTGCC 360
GGAGTTGCAA CAGTACTGGT CCGTCTTGAA CACGGT.GCAG GGGTTGTTGC ANCCGCCCGG 420 CGCCTTCANC GCCCCGGGGC ACTGCCCGTT GATNTCCGCC GCGCACCGGA TGCCGCGGCA 4 80
NCCGCCTGAC NTGGGGCTNA ATCCCATTGG CACGTTGAAA CCGTCNACCA AGGGAAATTT 540
CAAAAAATCC AAGTTGTTGA ACTGGTTGAA CGCCAACTCC NCCAGGGTNT TGGGCCGGTT 600
GCCNTACCCC GTGCATNACA ACACCCCGCC GCANTCCCCT TCTGGCACCG CCGCNTCCGC 660
TCCCCTCAAA AGAACACCCG TGCGGCCCAA AATCCGGNCC CCCGGTGGTT CCCCCTTTCN 720 CTTTAANGGT CCCCAATGCC CCTGNTTTAA CTGNCTCCCC CCCANGCNCG GCCCCGGCCC 780
ACNCTTTNTT AAAACATCNG TNCCNAAT 808
( 2 ) INFORMATION FOR SEQ ID NO : 47 :
( i ) SEQUENCE CHARACTERISTICS :
(A) LENGTH : 809 base pairs
( B ) TYPE : nucleic acid
( C ) STRANDEDNESS : single ( D ) TOPOLOGY : unknown
( i i ) MOLECULE TYPE : cDNA
( vi i ) IMMEDIATE SOURCE :
( B ) CLONE : U-D50
( xi ) SEQUENCE DESCRI PTION : SEQ I D NO : 47 : GGCACGAGTG TGCCTAACAG AGAAAGAGAG ACCGATAGCC TCCTCATTCA CTATGGCGAT 60
CCGATCGCCA GCTTCGCTGC TGTTATTTGC GTTTCTGATG CTTGCGCTCA CAGGAAGACT 120
GCAGGCCGGG CGCAGCTCGT GCATTGGCGT CTACTGGGGA CAAAACACAG ACGAGGGAAG 180
CTTAGCAGAT GCTTGTGCCA CAGGCAACTA CGAATACGTG AACATCGCCA CCCTTTTCAA 240
GTTTGGCATG GGCCAAACTC CANAGATCAA CCTCGCCGGC CACTGTGACC CTCGGAACAA 300
CGGCTGCGCG CGCTTAAGCA GAGAAATCCA GTCCTGCCAG GAGCGTGGAG TCACGGTGAT 360
GCTCTCCATC GGAGGTGGCG GGTCTTATGG CCTGAGTTCC ACCGAAGACG CCAAGGACGT 420 GGCGTCATAC CTCTGGCACA GTTTCTTGGG TGGTTCTGCT GCTCGCTACT CTCGACCCCT 480
CGGGGATGCG GTTCTGGATG GCATANACTT CAACATCNCC GGAGGGAGCA CAGAACACTA 540
TGATGAACTT GCCGCTTTCC TCAAGGGCTA CNACGAACAG GAAGCCGGAA CGAAAAAANT 600
TTTACTTGAA TGCTGCTCCC NCANTGTCCT TTCCCGGATT ACTGGCTTGG CACCCACTCA 660
NAAAANATCT CTTCCNACTT CCNTGTGGGT TGCANTTCCT CCAANAACCC TTCCTTGCCN 720 TTTCTCCCCA AAACCTATCC ATCTTGCAAT TCCTTTCACA AATTGGGTCN TNTCCNTCCC 780
NGCCCCNAAA ACTTTTTCCC TNGGGCTCC 809
(2) INFORMATION FOR SEQ ID NO: 48:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 809 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D86
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 48:
GGCACGAGCC ACAAGCTTGC CTTTGGTTGT GCCTAACAGA GAGAGAGAGA GAGACAGACC 60
GATAGCCTCC TCATTCACTA TGGCGATCCG ATCGCCAGCT TCGCTGCTGT TATTTGCGTT 120
TCTGATGCTT GCGCTCACAG GAAGACTGCA GGCCCGGCGC AACTCATGCA TTGGCGTCTA 180
CTGGGGACAA AAGACAGACG AGGGAAGCTT AGCAGATGCT TGTGCCACAG GCAACTACGA 240 ATACGTGAAC ATCGCCACCC TTTTCAAGTT TGGCATGGGC CAAACTCCAG AGATCAACCT 300
CGCTAGCCAC TGTGACCCTC AGAACAACGG CTGCGCGCGC TTAAGCAGCG AAATCCAGTC 360
CTGCCAGGAG CGTGGAGTCA AGGTGATGCT CTCCATCGGA GGTGGCGGGT CTTATGGCCT 420
GAGTTCCACC GAAGACGCCA AGGACGTGGC GTCATACCTC TGGCACAGTT TCTTGGGTGG 480
TTCTGCTGCT CGCTACTCTC GACCCCTCGG GGATGCGGTT CTGGATGGCA TAAACTTCAA 540 CATCNCCGGA GGGAGCACAG AACACTATGA TGAACTTGCC GCTTTCCTCA AGGGCTACAA 600
CGANCAGGAA GCCGGAACGA AAAAAGTTCA CTTGAATGCT GCTCCCCANT GTCTTTCCCG 660
GATTACTGGC TTGGCAACGC NCTCCAAAAC AAATCTCTTC CACTTCCTGT GGGTGCANTC 720
CTCCACAAAC CTTCNTGCAT TCTCCCCAAA CCTATCAATC TTGCNAAATG CGTTCAACAA 780 TTNGGGTCTT TTCATCCCCG CCCAAAACT 809
(2) INFORMATION FOR SEQ ID NO: 49:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 785 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single (D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D90
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 49:
GGCACGAGGA GAGACCGATA GCCTCCTCAT TCACTATGGC GATCCGATCG CCAGCTTCGC 60
TGCTGTTATT TGCGTTTCTG ATGCTTGCGC TCACAGGAAG ACTGCAGGCC GGGCGCAGCT 120
CGTGCATTGG CGTCTACTGG GGACAAAACA CAGACGAGGG AAGCTTAGCA GATGCTTGTG 180
CCACAGGCAA CTACGAATAC GTGAACATCG CCACCCTTTT CAAGTTTGGC ATGGGCCAAA 240 CTCCAGAGAT CAACCTCGCC GGCCACTGTG ACCCTCGGAA CAACGGCTGC GCGCGCTTAA 300
GCAGAGAAAT CCAGTCCTGC CAGGAGCGTG GAGTCACGGT GATGCTCTCC ATCGGAGGTG 360
GCGGGTCTTA TGGCCTGAGT TCCACCGAAG ACGCCAAGGA CGTGGCGTCA TACCTCTGGC 420
ACAGTTTCTT GGGTGGTTCT GCTGCTCGCT ACTCTCGACC CCTCGGGGAT GCGGTTCTGG 480
ATGGCATANA CTTCAACATC GCCGGAGGGA GCACAGAACA CTATGATGAA CTTGCCGCTT 540 TCCTCAAGGC CTACAACGAG CAGGAAGCCG GAACGAAAAA AGTTTACTTG AATTGCTGCT 600
CCGCANTATC CTTTCCNGAT TACTGGCTTG GCAACNCNCT CCAAAAAANA TCTCTTCCAC 660
TTCCTGTTGG GTGCAGTTCT TCCAANAACC CNTTCNTGCC ATTTCTCCCC AAAACGCTTT 720
CCATCTTTGC AAATGCCTTT CAACAATTGG GGTCTTGTTC CNNCCCTGCC CCAAAAACTG 780
TTCCT 785 ( 2 ) INFORMATION FOR SEQ ID NO : 50 :
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 813 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D93
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50:
GGCACGAGCG ATAGCCTCCT CATTCACTAT GGCGATCCGA TCGCCAACTT CGCTGCTGTT 60 ATTTGCGTTC CTGATGCTTG CGCTCACGGG AAGACTGCAG GCCCGGCCCA GCTCATGCAT 120 TGGCGTCTAC TGGGGACAAA ACACCGACGA GGGAAGCTTA GCAGATGCTT GTGCCACAGG 180
CAACTACGAT TACGTGAACA TCGCCACCCT TTTCAAGTTT GGCATGGGCC AAACTCCAGA 240
GATCAACCTC GCCGGCCACT GTGACCCTCG GAACAACGGC TGCGCGCGCT TGAGCAGCGA 300
AATCCAGTCC TGCCAGGAGC GTGGCGTCAA GGTGATGCTC TCCATCGGAG GTGGCGGGTC 360
TTATGGCCTG AGTTCCACCG AAGACGCCAA GGGAAGTAGC GTCATACCTC TGGCACAGTT 420
TCTTGGGTGG TTCTGCTGCT CGCTACTCGA GACCCCTCGG GGATGCGGAA CTGGATGGCA 480
TANACTTCAA CATCGCCGGA GGGAGAACAG AACACTATGA TGAACTTGCC GCTTTCCTCA 540 AGGCCTACAA CGANCAGGAA GCCGGAACNA AAAAAGTTCA CTTGAATTGC TGCTCCGCAG 600
TGTCCTTTCC CGGATTACTG GCTTGGCAAC GCACTCAGAA CANATCTCTT CNACTTCCTG 660
TGGGTGCAAT TTCCTCCACA ANCCTTCCNT GCCATTTCTC CCAAAAAGCT ATCNATCTTG 720
CAAATGCGTT CNACAATTGG GTCTTTTCCA NCCTGCNCAA AACTGTTCCT TGGGCTCCCG 780
CTGCCCCTGA AGGTGCNCCA ATTGGTGGCT NCT 813 (2) INFORMATION FOR SEQ ID NO: 51:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 819 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D61
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51:
GGCACTCAGG AACTCCAACA TCCAAGTCCT GTTGGATGTC CCCCGATCCG ACGTGCAGTC 60 ACTGGCCTCC AATCCTTCGG CCGCCGGCGA CTGGATCCGG AGGAACGTCG TCGCCTACTG 120
GCCCAGCGTC TCCTTTCGAT ACATAGCTGT CGGAAACGAG CTGATCCCCG GATCGGATCT 180
GGCGCAGTAC ATCCTCCCCG CCATGCGCAA CATCTACAAT GCTTTGTCCT CGGCTGGCCT 240
GCAAAACCAG ATCAAGGTCT CGACCGCGGT CGACACGGGC GTCCTCGGCA CGTCCTACCC 300
TCCCTCCGCC GGCGCCTTCT CCTCCGCCGC CCAGGCGTAC CTGAGCCCCA TCGTGCAGTT 360 CTTGGCGAGT AACGGAGCGC CGCTCCTGGT CAATGTGTAC CCTTATTTTA GCTACACCGG 420
CAACCCGGGA CAGATCTCGC TGCCCTACGC CCTGTTCACG GCCTCCGGCG TCGTCGTGCA 480
GGATGGGCGA TTCANCTATC AGAACCTGTT CGACGCCATC GTCGACGCGG TCTTCGCGGC 540
GCCTGGAAAA AATGGGAAGG GCGAACGTGG CGGTGGTGGT GTCCGAAAAC GGGTGGCCGT 600
CCGCGGGCGG AAGAACCCNA AACNAACACC ANCAACNCCG CCGGAANTTT CAACCANAAC 660 TTGATCAGGC ATGTTNGGCG GAAGAACCCC AAGGAAAACC AGGGAAGGAA ATCAAGGCTT 720
CNTNTTCCAA AANTTTCCAC CAAAAACCNA AAGGTGGANG GATCCAACAA AACTTTGGCC 780
TGTTTTATCC CAANNAACAN CCCNTCTTAC CNAATAACT 819
(2) INFORMATION FOR SEQ ID NO: 52: (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 794 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D63
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 52:
GGCACGAGGG ATTATAAAAA GAAACTCGCA CTCAGCCAGA TTGACTCAAG CATGAAGGTT 60 GCATCTTTCG AATGTTCCTT TTTTTTCCAA CATCTTTTGA ATGTTACATG ACAATCATGA 120
CGCCGTGCAT ACAATGATAC CTCTGATAGG GTTGAATGCA CAAACCACTC ATCGTGCTAT 180
ATTGCAAGTG CTTATGTTTG TATTCATTTT CTTATAATTT CAATGTGAGA GTTACCTTAG 240
GTTCATATTA NATTTATATA ATATTGGGCA TAGATTTTTT TGTCATTGTT TGTGATCTGC 300
AATTTCATTA GGTCATCGAC CATATTATGG TAAATTTAAT TTACTAAATT ATATCTTGAG 360 TTTATTGCTT TGCATATTTT GTGCTGGAAA CCATGCCAAT CCAACTCCTT TGCAAAACTT 420
AAGATAATTA AAGGAGATAT TGAGGATGCC GACTGCTTTA GGTTATTTGC ATTANACAAT 480
AAACGCCCGT GAAATGGAAA TCATGCAAAG CTTTGCATGG AGAATATGTT NATAGAGATA 540
GACATGAAGA CATGAATATG CTTTCTTGAT GAACAACGAT GTTCACNTGT TTACTGCATG 600
CATGATACAT CCAATGTTCT AGGCTTGTTG GCAATCATTT TATTCNAAAA ATTGTCTGTT 660 TCTCTNCCNC CATAAGGTTA GCTTGTGGAA AATGTTCAAC TTTGGGCNCA NATGATCCGT 720
TTTAGCAAAA TCCCNCCATT CCTATTTTTT TCCGGAATCC NNTTGGNAAA ANATCCTNAC 780
ATACTATTTC CACA 794
(2) INFORMATION FOR SEQ ID NO: 53:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 798 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
( ii) IMMEDIATE SOURCE: (B) CLONE: U-D65
(x ) SEQUENCE DESCRIPTION: SEQ ID NO: 53:
GGCACGAGGC CCGTAGAGCG AGTGCTGAGA TGGCTTTGAG GGCCTTCTTC CCCCTCTGCA 60
TCGCTCTCGT GGTGACGCTC TCACCTCTGT GTGATGCCAC TTCGCCTTAC TACACCATCA 120
CACCGCCCAC CCCCGTGGCC AAGCCGCCTT CAGTTGAACC ACCACCCTAC CACGGCCCTC 180 CGACGACCCA CCCTAAGCCA CCGAGTCATG GTGGCCAACC TCCGTCCCAC CATCACCCAA 240 CACCAATCTA CGGTGCACCC CCTCCGCAAC ACCACCACCA CCACCAACAC CACCACCAAC 300
CTGCACCACC AACTCACGCA GAACACCCTC CGTACTACCA CATGCCTTCC CCGCCGCCGC 360
ATGGCCAGCA CCCGTCACCA CCGTCACATG ATTATCCCGT ACCTCCTGCT CACAAGCCCC 420
GAACTCCGCC GCCGGTTTAC AAGTCTCCAC CACCGACCCA CCGTCCTTAC CCTCCATCGA 480
CGCCACCCCA CCATCCGACG CACCCGCCTT CTCAGCCGAC GCCGTCATAC AAGGCCCCGC 540
CACCATACAA GAACATCCCT GANCATCTCC ACCGCCGCGT CACTATCATT CTCCGTCTTC 600
ACCACCANCA CAACCACCAT NCAAATAGTC TCGTTTGCAT CTCTCCGTTG AANATGAACC 660 AATGTCNTTT AATAACGATC AGGGTTTCAA ATAAAAACNA ATTTCCGCCA TTGTAATGCT 720
ATGGTTGTTC TCTCTGCTTC CCGGGGAAAG TTTCTTGGGT CATNTTAACC NCCTCCTAAT 780
GTTCNGCTCT TNNTANAA 798 (2) INFORMATION FOR SEQ ID NO: 54:
(l) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 824 base pairs (B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D84
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54:
GGCACGAGCG CCCACCCCCG TGGCCAAGCC GCCTTCAGTT GAACCACCAC CCTACCACGG 60
CCCTCCGACG ACCCACCCTA AGCCACCGAG TCATGGTGGC CAACCTCCGT CCCACCATCA 120
CCCAACACCA ATCTACGGTG CACCCCCTCC GCAACACCAC CACCACCACC AACACCACCA 180 CCAACCTGCA CCACCAACTC ACGCAGAACA CCCTCCGTAC TACCACATGC CTTCCCCGCC 240
GCCGCATGGC CAGCACCCGT CACCACCGTC ACATGATTAT CCCGTACCTC CTGCTCACAA 300
GCCCCGAACT CCGCCGCCGG TTTACAAGTC TCCACCACCG ACCCACCGTC CTTACCCTCC 360
ATCGACGCCA CCCCACCATC CGACGCACCC GCCTTCTCAG CCGACGCCGT CATACAAGGC 420
CCCGCCANCA TNCNAGACCA TCCCTTGAGC ACTTCTCCAN CGNTTGCGTT TATTATCATT 480 CTCCGTCTTC ACCACCACCA CCACCACCAT ACAAATAGTC TCGTTTGCCA TCTCTCCGTT 540
GAAGATGACG CAGTGTCGTT TTAGTAGCGA TCAGGGTTAC CAATAAGAAC GATGTTGCCG 600
CCATTGTAAT GGCTATGGTT GTTCTCTCTG CTTTTCCGGG GGAAGGTTCT TTGGGTTCAT 660
GTTAAACCTC TCTCTTAAAT GTTCATGCAT CTTATTATAA ACNAAAATTG GCCATTTNNN 720
NNNTNTNNTN NNNNNTTNNN NNNNNAAAAC TCNAAAAATA TTTTTAAAAA CGGGCGGGGG 780 GCCCATCNAT TTTCCNNCCC GGGTNGGGGN TCCCAGNTTA TTNT 824
(2) INFORMATION FOR SEQ ID NO: 55:
(l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 797 base pairs
(B) TYPE: nucleic acid (C) STRANDEDNESS: single
(D) TOPOLOGY: unknown
(ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D75
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55:
GGCACGAGGG TTACGATCGT CATGTTGGGG GTGTTCAGCG GGGNAGGTGG TGGAGGTGCC 60
GGCGGAGCTG GTGGCCGCCG GCAGCAGGAC GCCGTCCCCT AAGACACGAG CGTCGGAGCT 120
GATCAACCGA TTCCTCGGGA GCTTCGCTCC CGCGGTGTCG ATCCAGATCG GGGACTTGGG 180 ACACCTCGCC TACTCCCACG CCAACCAGTC CCCCTTCGCT CCCAGGTTGT TTGCAGCGAA 240
GGACGANATT TACTGCCTCT TCAAGGGAGT GCTGACCAAC CTGGGCAGCT TGAGGCAGCA 300
GTATGGGCTT TCCAAGAGTG CCGACNAGGT GGTGCTGGTC ATCGAAGCCT ACAAGGCCCT 360
CCGTGACCGA GCTCCCTATC CTCCCAGCTT CATGCTCGCA CACCTTACTG GCAACTTCGC 420
CTTCGTGCTC TTCGACAAGT CCACATCATC CATCCTTGTT GCATCTGACC CANATGGAAN 480 ANTACCCTTG TTCTGGGGGA TCACTGCANA TGGATGCCTT GCCTTTGCTG ACNATCTANA 540
CTTGCTGAAG GGATCGTGCG GGAAGTCACT TGCACCATTC CCTGAAGGAT GTTACTATTC 600
CAATGCCTTG GGGGGCCTGA AAANCTATGA AAACCCCAAC ACAAGGTGAA CTGCTGTTTC 660
TTGAANATGA AGAANAATTN TTTGTGCCNC TTTCAAGGTG GAANGATCTG CCNTTCTTGC 720
GGNAACCCCC TAATCAAGGA ACATCTTCCA ATGTTTGGGG CNAATGATCC TCTTAAAATC 780 CTAAAATTCT TGGAATT 797
(2) INFORMATION FOR SEQ ID NO: 56:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 804 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D83
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56: GGCACGAGCT TCCACACAGG TGAGGGTAAG TCGGTTACGA TCGTCATGTT GGGGGTGTTC 60
AGCGGGGAGG TGGTGGAGGT GCCGGCGGAG CTGGTGGCCG CCGGCAGCAG GACGCCGTCC 120
CCTAAGACAC GGGCGTCGGA GCTGATCAAC CGATTCCTCG GGAGCTTCGC TCCCGCGGTG 180
TCGATCCAGA TCGGGGACTT GGGACACCTC GCCTACTCCC ACGCCAACCA GTCCCCCTTC 2 40
GCTCCCAGGT TGTTTGCAGC GAAGGACGAN ATTTACTGCC TCTTCAAGGG AGTGCTGACC 300 AACCTGGGCA GCTTGAGGCA GCAGTATGGG CTTTCCAAGA GTGCCGACNA GGTGGTGCTG 3 60 GTCATCGAAG CCTACAAGGC CCTCCGTGAC CGANCTCCCT ATCCTCCCAG CTTCATGCTC 420
GCACACCTTA TTGGCAACTT CGCCTTCGTG CTCTTCGACA AGTCCACATC ATCCATCCTT 480
GTTTGCATCT GACCCANATG GAAAAATGCC CTTGTTCTGG GGGATCACTG CANATGGATG 540
CCTTGCCTTT GCTGACNATC TANACTTGCT GAAGGGATCG TGCGGGAANT CACTTGCACA 600
TTCCCTGAAN GATGTTACTA TTCCAATGCC TTGGGGGGGC TGAAAANCTA TGAAAACCCA 660
ANCACAAGGT GACTGCTGTC TTGAANATAA AGAAAAATTT TTTGTGCCCC TTTCAAGGTT 720
GAANGATCTG CATTCTTGCG GCACCCCCTN ATCNAGGAAC NTCNTNCCAA TGTTGGGGCA 780 AATATCCTCT TTAAAANCAT AAAA 804
(2) INFORMATION FOR SEQ ID NO: 57:
(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 803 base pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE: (B) CLONE: U-D64
(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 57: GGCACGAGGG ATTATAAAAA GAAACTCGCA CTCAGCCAGA TTGACTCAAG CATGAAGGTT 60
GCATCTTTCG AATGTTCCTT TTTTTTCCAA CATCTTTTGA ATGTTACATG ACAATCATGA 120
CGCCGTGCAT ACAATGATAC CTCTGATAGG GTTGAATGCA CAAACCACTC ATCGTGCTAT 180
ATTGCAAGTG CTTATGTTTG TATTCATTTT CTTATAATTT CAATGTGAGA GTTACCTTAG 240
GTTCATATTA GATTTATATA ATATTGGGCA TAGATTTTTT TGTCATTGTT TGTGATCTGC 300 AATTTCATTA GGTCATCGAC CATATTATGG TAAATTTAAT TTACTAAATT ATATCTTGAG 360
TTTATTGCTT TGCATATTTT GTGCTGGAAA CCATGCCAAT CCAACTCCTT TGCAAAACTT 420
AAGATAATTA AAGGAGATAT TGAGGATGCC GACTGCTTTA GGTTATTTGC ATTANACAAT 480
AAACGCCCGT GAAATGGAAA TCATGCNAAG CTTTGCATGG AGAATATGTT NATAGANATA 540
GACATGAAGA CATGAATATG CTTTCTTGAT GAACAACGAA GTTCACNTGT TTACTGCATG 600 CATGATACAT CCAATGTTCT AGGCTTGTTA GGCAACCTTT ATCCAAAAAA TTGTCTGTAC 660
TCTCCCCCAT AAGGTAAGCC TGTGGAAAAT GTTCACTTTG GGCCNNATGA TCAGTTTANC 720
CGAAAATCCC CCTTCNTTAT TTGTTTTCTG AAACNCNTTG GAAANANATT CCTTACATAC 780
CTTTTTCACN NANATNTTGA ACC 803

Claims

1. A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID Nos 1 -57, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
2. A method according to claim 1 wherein the polynucleotide is obtained from the cDNA library having the NCIMB Accession Number 40814.
3. A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics.
4. A method according to claim 1 or 2 or 3, characterised in that the said polynucleotide modulates the production of pectate lyase.
5. A method according to claim 4 in which the polynucleotide sequence comprises at least one of the sequences depicted in the sequence listings as SEQ ID Nos. 13-18.
6. A method according to any preceding claim wherein the plant material is transformed using the Agrobacteήum, microparticle bombardment, fibre mediated or direct insertion method.
7. Plants, their progeny and seed and material obtained from said plants, produced according to a method as claimed in claims 1 to 6.
8. A vector functional in plants comprising a promoter region which is operable in plant cells, a polynucleotide sequence defined in claims 1 to 3 and a transcription termination sequence.
9. A vector according to claim 8 wherein the promoter is constitutive, developmentally regulated or switchable.
10. A vector according to claim 9 wherein the promoter is tissue specific or organ specific.
1 1. A genetically modified banana produced via the method according to claims 1 to 6 having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described in claims 1 to
3.
12. A method of controlling plant pathogens comprising the application of an anti- pathogenic agent to plants, characterised in that plants to which the said agent is applied are plants according to claim 7.
13. A polynucleotide associated with fruit ripening selected from SEQ ID Nos. 1 -57.
14. A fruit of a plant of the genus Musa having a retarded ripening phenotype generated by inserting into the genome of the said plant at least one of the polynucleotide(s) as claimed in claim 13.
15. A method, polynucleotide, plant, its progeny, seed and material obtained from said plants and a banana substantially as hereinbefore described with reference to the accompanying drawings and figures.
PCT/GB1997/002424 1996-09-10 1997-09-08 Genetic control of fruit ripening WO1998011228A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP09533834A JP2000517164A (en) 1996-09-10 1997-09-08 Genetic control of fruit ripeness
AU41291/97A AU4129197A (en) 1996-09-10 1997-09-08 Genetic control of fruit ripening
EP97939069A EP0931150A2 (en) 1996-09-10 1997-09-08 Genetic control of fruit ripening
CA002262505A CA2262505A1 (en) 1996-09-10 1997-09-08 Genetic control of fruit ripening

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9618862.8A GB9618862D0 (en) 1996-09-10 1996-09-10 Genetic control of fruit ripening
GBGB9708366.1A GB9708366D0 (en) 1997-04-25 1997-04-25 Genetic control of fruit ripening
GB9618862.8 1997-04-25
GB9708366.1 1997-04-25

Related Child Applications (2)

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US09/966,881 Continuation US20020120960A1 (en) 1996-09-10 2001-09-28 Genetic control of fruit ripening

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WO1998011228A3 WO1998011228A3 (en) 1998-04-23

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AU (1) AU4129197A (en)
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999007857A1 (en) * 1997-08-07 1999-02-18 Plant Bioscience Limited Pectin degrading enzymes
WO1999015668A2 (en) * 1997-09-25 1999-04-01 Boyce Thompson Institute For Plant Research, Inc. Banana proteins, dna, and dna regulatory elements associated with fruit development
WO2001029230A2 (en) * 1999-10-15 2001-04-26 Carnegie Institution Of Washington Engineering disease resistance with pectate lyase-like genes
WO2013022757A2 (en) * 2011-08-05 2013-02-14 The United States Of America, As Represented By The Secretary Of Agriculture Banana mads-box genes for banana ripening control

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994021794A1 (en) * 1993-03-22 1994-09-29 Zeneca Limited Dna, dna constructs, cells and plants derived therefrom
WO1995007993A1 (en) * 1993-09-13 1995-03-23 Zeneca Limited Regulation of senescence
WO1995015678A1 (en) * 1993-12-09 1995-06-15 The Texas A & M University System Agrobacterium tumefaciens transformation of musa species

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994021794A1 (en) * 1993-03-22 1994-09-29 Zeneca Limited Dna, dna constructs, cells and plants derived therefrom
WO1995007993A1 (en) * 1993-09-13 1995-03-23 Zeneca Limited Regulation of senescence
WO1995015678A1 (en) * 1993-12-09 1995-06-15 The Texas A & M University System Agrobacterium tumefaciens transformation of musa species

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CLENDENNEN S. AND MAY G.: "Differential gene expression in ripening banana fruit" PLANT PHYSIOLOGY, vol. 115, no. 2, October 1997, pages 463-469, XP002049417 *
CLENDENNEN S. ET AL.: "Isolation and identification of genes differentially expressed during banana fruit ripening" PLANT PHYSIOLOGY, vol. 111, no. 2,suppl., June 1996, page 34 XP002049413 *
DOMINGUEZ-PUIGJANER E. ET AL.: "Differential protein accumulation in banana fruit ripening" PLANT PHYSIOLOGY, vol. 98, no. 1, January 1992, pages 157-162, XP002049414 *
MEDINA-SUAREZ R. ET AL.: "Gene expression in banana peel and pulp during ripening" PLANT PHYSIOLOGY, vol. 111, no. 2, Suppl., June 1996, page 122 XP002049412 *
MEDINA-SUAREZ R. ET AL.: "Gene expression in the pulp of ripening bananas" PLANT PHYSIOLOGY, vol. 115, no. 2, October 1997, pages 453-461, XP002049416 *
SEYMOUR B.: "Banana" BIOCHEMISTRY OF FRUIT RIPENING, 1993, pages 83-106, XP002049415 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999007857A1 (en) * 1997-08-07 1999-02-18 Plant Bioscience Limited Pectin degrading enzymes
WO1999015668A2 (en) * 1997-09-25 1999-04-01 Boyce Thompson Institute For Plant Research, Inc. Banana proteins, dna, and dna regulatory elements associated with fruit development
WO1999015668A3 (en) * 1997-09-25 1999-10-07 Thompson Boyce Plant Res Banana proteins, dna, and dna regulatory elements associated with fruit development
US6284946B1 (en) 1997-09-25 2001-09-04 Boyce Thompson Institute For Plant Research Inc. Banana DNA associated with fruit development
WO2001029230A2 (en) * 1999-10-15 2001-04-26 Carnegie Institution Of Washington Engineering disease resistance with pectate lyase-like genes
WO2001029230A3 (en) * 1999-10-15 2002-01-17 Carnegie Inst Of Washington Engineering disease resistance with pectate lyase-like genes
WO2013022757A2 (en) * 2011-08-05 2013-02-14 The United States Of America, As Represented By The Secretary Of Agriculture Banana mads-box genes for banana ripening control
WO2013022757A3 (en) * 2011-08-05 2013-05-16 The United States Of America, As Represented By The Secretary Of Agriculture Banana mads-box genes for banana ripening control

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AU4129197A (en) 1998-04-02
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WO1998011228A3 (en) 1998-04-23

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