WO1998007821A1 - Compositions detergentes possedant une activite cellulolytique regulee par anticorps - Google Patents

Compositions detergentes possedant une activite cellulolytique regulee par anticorps Download PDF

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Publication number
WO1998007821A1
WO1998007821A1 PCT/US1996/013370 US9613370W WO9807821A1 WO 1998007821 A1 WO1998007821 A1 WO 1998007821A1 US 9613370 W US9613370 W US 9613370W WO 9807821 A1 WO9807821 A1 WO 9807821A1
Authority
WO
WIPO (PCT)
Prior art keywords
cellulase
detergent
alkyl
antibody
compositions
Prior art date
Application number
PCT/US1996/013370
Other languages
English (en)
Inventor
Ivan Maurice Alfons Jan Herbots
Jean Wevers
Tim Maria Joris Van Hauwermeiren
Original Assignee
The Procter & Gamble Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Procter & Gamble Company filed Critical The Procter & Gamble Company
Priority to AU69537/96A priority Critical patent/AU6953796A/en
Priority to PCT/US1996/013370 priority patent/WO1998007821A1/fr
Priority to EP97936031A priority patent/EP0918839A1/fr
Priority to CN97198595.2A priority patent/CN1232492A/zh
Priority to BR9711626-2A priority patent/BR9711626A/pt
Priority to JP10510727A priority patent/JP2000500818A/ja
Priority to CA002263430A priority patent/CA2263430A1/fr
Priority to PCT/US1997/011544 priority patent/WO1998007823A1/fr
Priority to ARP970103732A priority patent/AR009261A1/es
Publication of WO1998007821A1 publication Critical patent/WO1998007821A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/384—Animal products
    • C11D3/3845—Antibodies
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase

Definitions

  • the present invention relates to laundry detergent compositions comprising a cellulase-directed antibody and a cellulase in order to prevent potential tensile strength loss related to the hydrolytic activity of cellulase on cellulose substrates while maintaining the desired benefits from the use of cellulase.
  • Heavy and light chains are indeed composed of constant and variable domains.
  • the constant domains are very important for a number of functions, but for many applications in industrial processes and products their variable domains are sufficient. Consequently many methods have been described to produce antibody fragments.
  • the antibodies and release agents can be contained in a granulate.
  • Said antibody granulate can suitably contain various granulation aids, binders, fillers, plasticizers, lubricants, cores and the like.
  • granulation aids include cellulose (e.g. cellulosic fibers or in microcrystalline form) , cellulose derivatives (CMC, MC, HPC, HPMC) , gelatin, starch, dextrins, sugars, polyvinylpyrrolidone, PVA, PEG, salts (e.g. sodium sulfate, calcium sulfate) , titanium dioxide, talc, clays (kaolin or bentonite) and nonionic surfactants.
  • Other materials of relevance for incorporation in the granulate are described in EP 304 331.
  • the cellulases having a non-degrading index (NDI) of not less than 500 and being alkalophilic cellulases having an optimum pH not less than 7 or whose relative activity at a pH of not less than 8 is 50% or over of the activity under optimum conditions when carboxy methyl cellulose (CMC) is used as a substrate, or an endoglucanase component which is immunoreactive with an antibody raised against a highly purified ⁇ 43kD endoglucanase derived from Humicola insolens, DSM 1800, or which is a homologue or derivative of the ⁇ 43kD endoglucanase exhibiting cellulase activity; a preferred endoglucanase component has the amino acid sequence disclosed in PCT Patent Application No.
  • y is 2-4, preferably 3. whereby R2 is H or a C1-C3 alkyl, whereby x is 0-4, preferably 0-2, most preferably 0, whereby R3 , R4 and R5 are either the same or different and can be either a short chain alkyl (C1-C3) or alkoxylated alkyl of the formula III,
  • R2R3 and R4 are methyl and hydroxyethyl groups and the anion X may be selected from halide, methosulphate , acetate and phosphate ions.
  • suitable quaternary ammonium compounds of formulae (i) for use herein are : coconut trimethyl ammonium chloride or bromide; coconut methyl dihydroxyethyl ammonium chloride or bromide ; decyl triethyl ammonium chloride; decyl dimethyl hydroxyethyl ammonium chloride or bromide;
  • Preferred amines according to the formula herein above are n-alkyl amines. Suitable amines for use herein may be selected from 1-hexylamine, 1-octylamine, 1-decylamine and laurylamine . Other preferred primary amines include C8-C10 oxypropylamine , octyloxypropylamine, 2 -ethylhexyl- oxypropylamine, lauryl amido propylamine and amido propylamine .
  • Preferred tertiary amines are R1R2R3N where Rl is a C6-C12 alkyl chain, R2 and R3 are C1-C3 alkyl or
  • the most preferred amines for use in the compositions herein are 1-hexylamine, 1-octylamine, 1-decylamine, 1- dodecylamine .
  • Especially desirable are n- dodecyldimethylamine and bishydroxyethylcoconutalkylamine and oleylamine 7 times ethoxylated, lauryl amido propylamine and cocoamido propylamine .
  • amylases include Purafact Ox Am R described in WO 94/18314, published August 18, 1994; WO96/05295, Genencor, published February 22, 1995 and amylase variants having additional modification in the immediate parent available from Novo Nordisk A/S, disclosed in WO 95/10603, published April 95.
  • Preferred builder systems include a mixture of a water- insoluble aluminosilicate builder such as zeolite A, and a watersoluble carboxylate chelating agent such as citric acid.
  • compositions can comprise a silicone/silica mixture in combination with fumed nonporous silica such as Aerosil R .
  • polyethylene glycols particularly those of molecular weight 1000-10000, more particularly 2000 to 8000 and most preferably about 4000. These are used at levels of from 0.20% to 5% more preferably from 0.25% to 2.5% by weight. These polymers and the previously mentioned homo- or co-polymeric polycarboxylate salts are valuable for improving whiteness maintenance, fabric ash deposition, and cleaning performance on clay, proteinaceous and oxidizable soils in the presence of transition metal impurities.
  • modified polyesters as random copolymers of dimethyl terephthalate, dimethyl sulfoisophthalate, ethylene glycol and 1-2 propane diol, the end groups consisting primarily of sulphobenzoate and secondarily of mono esters of ethylene glycol and/or propane-diol .
  • the target is to obtain a polymer capped at both end by sulphobenzoate groups, "primarily", in the present context most of said copolymers herein will be end- capped by sulphobenzoate groups.
  • Alkoxylated polycarboxylates such as those prepared from polyacrylates are useful herein to provide additional grease removal performance. Such materials are described in WO 91/08281 and PCT 90/01815 at p. 4 et seq. , incorporated herein by reference. Chemically, these materials comprise polyacrylates having one ethoxy side- chain per every 7-8 acrylate units.
  • the side-chains are of the formula - (CH2CH2O) m (CH2) n CH 3 wherein m is 2-3 and n is 6-12.
  • the side-chains are ester- linked to the polyacrylate "backbone” to provide a "comb" polymer type structure.
  • the molecular weight can vary, but is typically in the range of about 2000 to about 50,000.
  • Levels of smectite clay are normally in the range from 2% to 20%, more preferably from 5% to 15% by weight, with the material being added as a dry mixed component to the remainder of the formulation.
  • Organic fabric softening agents such as the water-insoluble tertiary amines or dilong chain amide materials are incorporated at levels of from 0.5% to 5% by weight, normally from 1% to 3% by weight whilst the high molecular weight polyethylene oxide materials and the water soluble cationic materials are added at levels of from 0.1% to 2%, normally from 0.15% to 1.5% by weight.
  • These materials are normally added to the spray dried portion of the composition, although in some instances it may be more convenient to add them as a dry mixed particulate, or spray them as molten liquid on to other solid components of the composition.
  • the detergent composition of the present invention can also contain dispersants : Suitable water-soluble organic salts are the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms .
  • copolymer of acrylate and methylacrylate such as the 480N having a molecular weight of 4000, at a level from 0.5-20% by weight of composition can be added in the detergent compositions of the present invention.
  • R wherein P is a polymerisable unit, whereto the R-N-0 group can be attached to or wherein the R-N-0 group forms part of the polymerisable unit or a combination of both.
  • Silicone Polydimethylsiloxane foam controller antifoam with siloxane-oxyalkylene copolymer as dispersing agent with a ratio of said foam controller to said dispersing agent Of 10 :1 to 100:1.
  • the Carezyme solution was diluted in the Tris buffer to a final concentration of 0.6 CEVU/ml .
  • the carezyme preparation was incubated with the antibody solution (0.18 mg/ml) during 60 minutes at 30°C, 40°C and 50°C.
  • the detergent solution already contained Carezyme at an initial level of 0.6 CEVU/ml and the antibody was subsequently thereto added.
  • laundry detergent compositions were prepared in accord with the invention:
  • Brightener 1 0.08 0.19 0.19 Brightener 2 0.04 0.04 Encapsulated perfume 0.3 0.3 0.3 particles Silicone antifoam 0.5 2.4 2.4 Minors/misc to 100%
  • Syndet bar fabric cleaning compositions were prepared in accord with the present invention :
  • Detergent additives were prepared in accord with the present invention :

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Detergent Compositions (AREA)

Abstract

La présente invention se rapporte à des compositions détergentes comportant un anticorps dirigé contre la cellulase et de la cellulase. Ces compositions permettent d'empêcher la perte de résistance à la traction éventuelle liée à l'activité hydrolytique de la cellulase sur des substrats de cellulose et elles préservent simultanément les avantages souhaités liés à l'utilisation de la cellulase.
PCT/US1996/013370 1996-08-16 1996-08-16 Compositions detergentes possedant une activite cellulolytique regulee par anticorps WO1998007821A1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU69537/96A AU6953796A (en) 1996-08-16 1996-08-16 Detergent compositions comprising antibody controlled cellulolytic activity
PCT/US1996/013370 WO1998007821A1 (fr) 1996-08-16 1996-08-16 Compositions detergentes possedant une activite cellulolytique regulee par anticorps
EP97936031A EP0918839A1 (fr) 1996-08-16 1997-08-13 Compositions detergentes ayant une activite cellulolytique regulee par un anticorps
CN97198595.2A CN1232492A (zh) 1996-08-16 1997-08-13 含有抗体的控制纤维素活性的洗涤剂组合物
BR9711626-2A BR9711626A (pt) 1996-08-16 1997-08-13 "composições detergentes compreendendo atividade celulolìtica controlada por anticorpo"
JP10510727A JP2000500818A (ja) 1996-08-16 1997-08-13 抗体で制御されるセルロース分解活性を有する洗剤組成物
CA002263430A CA2263430A1 (fr) 1996-08-16 1997-08-13 Compositions detergentes ayant une activite cellulolytique regulee par un anticorps
PCT/US1997/011544 WO1998007823A1 (fr) 1996-08-16 1997-08-13 Compositions detergentes ayant une activite cellulolytique regulee par un anticorps
ARP970103732A AR009261A1 (es) 1996-08-16 1997-08-15 Composicion detergente que comprende una celulasa y un anticuerpo direccional contra la celulasa, aditivo detergente y su uso para controlarla actividad celulitico-enzimatica

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1996/013370 WO1998007821A1 (fr) 1996-08-16 1996-08-16 Compositions detergentes possedant une activite cellulolytique regulee par anticorps

Publications (1)

Publication Number Publication Date
WO1998007821A1 true WO1998007821A1 (fr) 1998-02-26

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1996/013370 WO1998007821A1 (fr) 1996-08-16 1996-08-16 Compositions detergentes possedant une activite cellulolytique regulee par anticorps
PCT/US1997/011544 WO1998007823A1 (fr) 1996-08-16 1997-08-13 Compositions detergentes ayant une activite cellulolytique regulee par un anticorps

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US1997/011544 WO1998007823A1 (fr) 1996-08-16 1997-08-13 Compositions detergentes ayant une activite cellulolytique regulee par un anticorps

Country Status (8)

Country Link
EP (1) EP0918839A1 (fr)
JP (1) JP2000500818A (fr)
CN (1) CN1232492A (fr)
AR (1) AR009261A1 (fr)
AU (1) AU6953796A (fr)
BR (1) BR9711626A (fr)
CA (1) CA2263430A1 (fr)
WO (2) WO1998007821A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066696A1 (fr) * 1999-04-29 2000-11-09 Genencor International, Inc. Matrice detergente a base de cellulase
WO2001036577A1 (fr) * 1999-11-16 2001-05-25 Unilever N.V. Procede de rinçage de textiles

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010063457A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Cellulase

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298654A2 (fr) * 1987-07-07 1989-01-11 Hybrisens Ltd. Utilisation des interactions entre anticorps et antigènes pour protéger ou moduler l'activité biologique
WO1991006574A1 (fr) * 1989-11-02 1991-05-16 Novo Nordisk A/S Procede de preparation d'acides peroxycarboxyliques
WO1991017243A1 (fr) * 1990-05-09 1991-11-14 Novo Nordisk A/S Preparation de cellulase comprenant un enzyme d'endoglucanase
WO1994007998A1 (fr) * 1992-10-06 1994-04-14 Novo Nordisk A/S Variantes de cellulase
WO1994021801A2 (fr) * 1993-03-17 1994-09-29 Genencor International, Inc. Purification et clonage moleculaire de la cellulase eg iii
WO1995024471A1 (fr) * 1994-03-08 1995-09-14 Novo Nordisk A/S Nouvelles cellulases alcalines

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298654A2 (fr) * 1987-07-07 1989-01-11 Hybrisens Ltd. Utilisation des interactions entre anticorps et antigènes pour protéger ou moduler l'activité biologique
WO1991006574A1 (fr) * 1989-11-02 1991-05-16 Novo Nordisk A/S Procede de preparation d'acides peroxycarboxyliques
WO1991017243A1 (fr) * 1990-05-09 1991-11-14 Novo Nordisk A/S Preparation de cellulase comprenant un enzyme d'endoglucanase
WO1994007998A1 (fr) * 1992-10-06 1994-04-14 Novo Nordisk A/S Variantes de cellulase
WO1994021801A2 (fr) * 1993-03-17 1994-09-29 Genencor International, Inc. Purification et clonage moleculaire de la cellulase eg iii
WO1995024471A1 (fr) * 1994-03-08 1995-09-14 Novo Nordisk A/S Nouvelles cellulases alcalines

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066696A1 (fr) * 1999-04-29 2000-11-09 Genencor International, Inc. Matrice detergente a base de cellulase
US6565613B1 (en) 1999-04-29 2003-05-20 Genencor International, Inc. Cellulase detergent matrix
WO2001036577A1 (fr) * 1999-11-16 2001-05-25 Unilever N.V. Procede de rinçage de textiles

Also Published As

Publication number Publication date
CA2263430A1 (fr) 1998-02-26
AU6953796A (en) 1998-03-06
AR009261A1 (es) 2000-04-12
CN1232492A (zh) 1999-10-20
JP2000500818A (ja) 2000-01-25
EP0918839A1 (fr) 1999-06-02
WO1998007823A1 (fr) 1998-02-26
BR9711626A (pt) 2000-10-17

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