WO1998002550A3 - Mikrobielle erzeugung von isopren - Google Patents

Mikrobielle erzeugung von isopren Download PDF

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Publication number
WO1998002550A3
WO1998002550A3 PCT/DE1997/001498 DE9701498W WO9802550A3 WO 1998002550 A3 WO1998002550 A3 WO 1998002550A3 DE 9701498 W DE9701498 W DE 9701498W WO 9802550 A3 WO9802550 A3 WO 9802550A3
Authority
WO
WIPO (PCT)
Prior art keywords
micro
organism
isoprene
microbial
synthase gene
Prior art date
Application number
PCT/DE1997/001498
Other languages
English (en)
French (fr)
Other versions
WO1998002550A2 (de
Inventor
Wolfgang Zimmer
Original Assignee
Fraunhofer Ges Forschung
Wolfgang Zimmer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung, Wolfgang Zimmer filed Critical Fraunhofer Ges Forschung
Publication of WO1998002550A2 publication Critical patent/WO1998002550A2/de
Publication of WO1998002550A3 publication Critical patent/WO1998002550A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung bezieht sich auf einen Mikroorganismus, eine Verwendung dieses Mikroorganismus zur Erzeugung von Isopren sowie auf ein Verfahren zu seiner Herstellung. Mit Hilfe eines mit geeigneten Oligonukleotiden amplifizierten Genfragmentes wird das Isoprensynthasegen, beispielsweise der Stieleiche, identifiziert und in einen Mikroorganismus kloniert. Der Mikroorganismus, der ein Isoprensynthasegen enthält, wird kultiviert und das freigesetzte Isopren über fraktionierte Kühlung aus dem Gasraum über der Kultur abgetrennt.
PCT/DE1997/001498 1996-07-15 1997-07-11 Mikrobielle erzeugung von isopren WO1998002550A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19629568.8 1996-07-15
DE19629568A DE19629568C1 (de) 1996-07-15 1996-07-15 Verfahren zur Herstellung von Isopren

Publications (2)

Publication Number Publication Date
WO1998002550A2 WO1998002550A2 (de) 1998-01-22
WO1998002550A3 true WO1998002550A3 (de) 1998-02-19

Family

ID=7800518

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1997/001498 WO1998002550A2 (de) 1996-07-15 1997-07-11 Mikrobielle erzeugung von isopren

Country Status (2)

Country Link
DE (1) DE19629568C1 (de)
WO (1) WO1998002550A2 (de)

Families Citing this family (37)

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US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
US8609385B2 (en) 2007-05-01 2013-12-17 Zuvachem, Inc. Methods for the direct conversion of carbon dioxide into a hydrocarbon using a metabolically engineered photosynthetic microorganism
MX2010006576A (es) 2007-12-13 2010-10-15 Danisco Us Inc Composiciones y metodos para producir isopreno.
MX2010011706A (es) * 2008-04-23 2011-03-15 Danisco Us Inc Variantes de isopreno-sintasa para produccion microbiana mejorada de isopreno.
MY156256A (en) 2008-07-02 2016-01-29 Danisco Us Inc Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges
ZA201102111B (en) 2008-09-15 2013-10-30 Danisco Us Inc Reduction of carbon dioxide emission during isoprene production by fermentation
US20100184178A1 (en) * 2008-09-15 2010-07-22 Zachary Quinn Beck Increased isoprene production using mevalonate kinase and isoprene synthase
BRPI0918453A2 (pt) * 2008-09-15 2019-12-17 Danisco Us Inc sistemas usando cultura de células para a produção de isopreno
MY169163A (en) 2008-09-15 2019-02-19 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
WO2010031076A2 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Conversion of prenyl derivatives to isoprene
MY161071A (en) 2008-12-30 2017-04-14 Danisco Us Inc Methods of producing isoprene and a co-product
EP2421966A2 (de) 2009-04-23 2012-02-29 Danisco US Inc. Dreidimensionale struktur von isopren-synthase und verwendung davon zur erzeugung von varianten
TWI427149B (zh) 2009-06-17 2014-02-21 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
EP2516657A1 (de) 2009-12-22 2012-10-31 Danisco US Inc. Membranbioreaktor für erhöhte produktion von isoprengas
WO2011079314A2 (en) 2009-12-23 2011-06-30 Danisco Us Inc. Compositions and methods of pgl for the increased production of isoprene
BR112012032276A2 (pt) 2010-06-17 2016-11-16 Danisco Us Inc composições de combustível compreendendo derivados de isopreno
WO2012019169A1 (en) 2010-08-06 2012-02-09 Danisco Us Inc. Production of isoprene under neutral ph conditions
JP2014502148A (ja) 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体
WO2012088450A1 (en) 2010-12-22 2012-06-28 Danisco Us Inc. Biological production of pentose sugars using recombinant cells
BR112013018316A2 (pt) 2010-12-22 2018-09-11 Danisco Us Inc composições e métodos para produção aprimorada de isopreno usando dois tipos de enzimas ispg
ES2699747T3 (es) 2011-04-29 2019-02-12 Danisco Us Inc Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides
EP2702139A1 (de) 2011-04-29 2014-03-05 Danisco US Inc. Herstellung von mevalonat, isopren und isoprenoiden mit für polypeptide mit enzymatischen thiolase-, hmg-coa-synthase- und hmg-coa-reduktase-aktivitäten codierenden genen
SG2014007991A (en) 2011-08-04 2014-03-28 Danisco Us Inc Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase
WO2013022799A1 (en) 2011-08-05 2013-02-14 Danisco Us Inc. PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS
SG11201401249PA (en) 2011-10-07 2014-05-29 Danisco Us Inc Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
WO2013063528A2 (en) 2011-10-27 2013-05-02 Danisco Us Inc. Isoprene synthase variants with improved solubility for production of isoprene
BR112014014947A2 (pt) 2011-12-23 2020-10-27 Danisco Us Inc. produção acentuada de isopreno com o uso de células hospedeiras tendo atividade de ispa diminuida
US8865442B2 (en) 2011-12-23 2014-10-21 Danisco Us Inc. Production of isoprene under reduced oxygen inlet levels
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US8895277B2 (en) 2012-05-02 2014-11-25 Danisco Us Inc. Legume isoprene synthase for production of isoprene
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
US20160002672A1 (en) 2012-12-21 2016-01-07 Danisco Us Inc. Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway
EP2984105B1 (de) 2013-04-10 2019-05-22 Danisco US Inc. Phosphoketolasen zur verbesserten herstellung von aus dem acetylcoenzym a gewonnenen metaboliten, isopren, isoprenoidvorläufern und isoprenoiden
EP3011018B1 (de) 2013-06-21 2019-03-27 Danisco US Inc. Zusammensetzungen und verfahren für clostridiatransformation
DK3107993T3 (da) 2014-02-20 2020-11-23 Danisco Us Inc Rekombinante mikroorganismer til forbedret fremstilling af mevalonat, isopren, isoprenoide forløbere, isoprenoider og acetyl-coa-afledte produkter

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983729A (en) * 1989-10-19 1991-01-08 The Goodyear Tire & Rubber Company DNA fragment encoding a rubber polymerase and its use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983729A (en) * 1989-10-19 1991-01-08 The Goodyear Tire & Rubber Company DNA fragment encoding a rubber polymerase and its use

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KUZMA J. AND FALL R.: "Leaf isoprene emission is dependent on leaf development and the level of isoprene synthase", PLANT PHYSIOLOGY, vol. 101, no. 2, February 1993 (1993-02-01), pages 435 - 440, XP002050256 *
SCHNITZLER J. ET AL.: "Characterization of an isoprene synthase from leaves of Quercus petraea (Mattuschka) Liebl", BOTANICA ACTA, vol. 109, no. 3, 1996, pages 216 - 221, XP002050255 *
SILVER G. AND FALL R.: "Characterization of Aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 22, 2 June 1995 (1995-06-02), pages 13010 - 13016, XP002050254 *
SILVER G. AND FALL R.: "Enzymatic synthesis of isoprene from dimethylallyl diphosphate in Aspen leaf extracts", PLANT PHYSIOLOGY, vol. 97, no. 4, December 1991 (1991-12-01), pages 1588 - 1591, XP002050257 *

Also Published As

Publication number Publication date
WO1998002550A2 (de) 1998-01-22
DE19629568C1 (de) 1998-01-08

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