WO1997047766A1 - Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry - Google Patents

Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry Download PDF

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Publication number
WO1997047766A1
WO1997047766A1 PCT/US1997/008518 US9708518W WO9747766A1 WO 1997047766 A1 WO1997047766 A1 WO 1997047766A1 US 9708518 W US9708518 W US 9708518W WO 9747766 A1 WO9747766 A1 WO 9747766A1
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Prior art keywords
mass
amplified dna
mass spectrometry
electrospray ionization
desalting
Prior art date
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PCT/US1997/008518
Other languages
English (en)
French (fr)
Inventor
Chris E. Hopkins
Raymond F. Gesteland
Andy Peiffer
Dora Stauffer
Mark Leppert
Pamela F. Crain
James A. Mccloskey
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University Of Utah Research Foundation
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Application filed by University Of Utah Research Foundation filed Critical University Of Utah Research Foundation
Priority to JP10501607A priority Critical patent/JP2000512497A/ja
Priority to EP97930985A priority patent/EP0954611A1/en
Publication of WO1997047766A1 publication Critical patent/WO1997047766A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
PCT/US1997/008518 1996-06-10 1997-06-10 Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry WO1997047766A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP10501607A JP2000512497A (ja) 1996-06-10 1997-06-10 エレクトロスプレー質量分析法による迅速で正確な変異体ｄｎａ配列の同定
EP97930985A EP0954611A1 (en) 1996-06-10 1997-06-10 Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1970296P 1996-06-10 1996-06-10
US60/019,702 1996-06-10

Publications (1)

Publication Number Publication Date
WO1997047766A1 true WO1997047766A1 (en) 1997-12-18

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ID=21794595

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/008518 WO1997047766A1 (en) 1996-06-10 1997-06-10 Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry

Country Status (4)

Country Link
EP (1) EP0954611A1 (ja)
JP (1) JP2000512497A (ja)
CA (1) CA2257866A1 (ja)
WO (1) WO1997047766A1 (ja)

Cited By (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2336206A (en) * 1998-01-27 1999-10-13 Bruker Daltonik Gmbh Method of enzymatic replication of genetic material in preparation for mass spectrometric analysis
GB2339279A (en) * 1998-05-29 2000-01-19 Bruker Daltonik Gmbh Mutation analysis using mass spectrometry
EP1176212A1 (en) * 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
EP1252336A1 (en) * 2000-02-02 2002-10-30 Advion BioSciences, Inc. Detection of single nucleotide polymorphisms
WO2004009849A1 (en) * 2002-07-19 2004-01-29 Isis Pharmaceuticals, Inc. Methods for mass spectrometry analysis utilizing an integrated microfluidics sample platform
EP2088209A1 (en) * 1999-10-13 2009-08-12 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
US7956175B2 (en) 2003-09-11 2011-06-07 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US20110137022A1 (en) * 2008-08-20 2011-06-09 Dennis Paul Michaud Method for the analysis of oligonucleotides
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8017358B2 (en) 2001-03-02 2011-09-13 Ibis Biosciences, Inc. Method for rapid detection and identification of bioagents
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US8173957B2 (en) 2004-05-24 2012-05-08 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
US8187814B2 (en) 2004-02-18 2012-05-29 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8214154B2 (en) 2001-03-02 2012-07-03 Ibis Biosciences, Inc. Systems for rapid identification of pathogens in humans and animals
US8268565B2 (en) 2001-03-02 2012-09-18 Ibis Biosciences, Inc. Methods for identifying bioagents
US8298760B2 (en) 2001-06-26 2012-10-30 Ibis Bioscience, Inc. Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby
US8407010B2 (en) 2004-05-25 2013-03-26 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US8563250B2 (en) 2001-03-02 2013-10-22 Ibis Biosciences, Inc. Methods for identifying bioagents
US8822156B2 (en) 2002-12-06 2014-09-02 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US8871471B2 (en) 2007-02-23 2014-10-28 Ibis Biosciences, Inc. Methods for rapid forensic DNA analysis
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US9873906B2 (en) 2004-07-14 2018-01-23 Ibis Biosciences, Inc. Methods for repairing degraded DNA
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
CN113881810A (zh) * 2021-11-02 2022-01-04 南方科技大学 一种新型冠状病毒病原微生物检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221518A (en) * 1984-12-14 1993-06-22 Mills Randell L DNA sequencing apparatus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221518A (en) * 1984-12-14 1993-06-22 Mills Randell L DNA sequencing apparatus

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CARCINOGENESIS, 1996, Vol. 17, No. 5, CHAUDHARY et al., "Characterization of an N6-Oxopropenyl-2'-Deoxyadenosine Adduct in Malondialdehyde-Modified DNA Using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry", pages 1167-1170. *
EPILEPSIA, 1994, Vol. 35, Supplement 1, DELGADO-ESCUETA et al., "Progress in Mapping Epilepsy Genes", pages S29-S40. *
EUROPEAN JOURNAL OF CANCER, 1994, Vol. 30A, No. 11, DOBBIE et al., "Mutational Analysis of the First 14 Exons of the Adenomatous Polyposis Coli (APC) Gene", pages 1709-1713. *
PROCEEDINGS OF THE 41ST ASMA CONFERENCE ON MASS SPECTROMETRY AND ALLIED TOPICS, June 1993, HETTICH et al., "MALDI-FTMS for the Characterization of Ultraviolet and X-Ray Damage to Nucleic Acid Constituents", pages 250A and 250B. *
PROCEEDINGS OF THE 43RD ASMS CONFERENCE ON MASS SPECTROMETRY AND ALLIED TOPICS, 1995, ZHAO et al., "Capillary Isotachophoresis-Mass Spectrometric Determination of DNA Damage", page 592. *

Cited By (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2336206A (en) * 1998-01-27 1999-10-13 Bruker Daltonik Gmbh Method of enzymatic replication of genetic material in preparation for mass spectrometric analysis
GB2339279A (en) * 1998-05-29 2000-01-19 Bruker Daltonik Gmbh Mutation analysis using mass spectrometry
US6503710B2 (en) 1998-05-29 2003-01-07 Ivo Glynne Gut Mutation analysis using mass spectrometry
GB2339279B (en) * 1998-05-29 2003-11-19 Bruker Daltonik Gmbh Mutation analysis using mass spectrometry
EP2088209A1 (en) * 1999-10-13 2009-08-12 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
EP1252336A1 (en) * 2000-02-02 2002-10-30 Advion BioSciences, Inc. Detection of single nucleotide polymorphisms
EP1252336A4 (en) * 2000-02-02 2005-02-09 Advion Biosciences Inc DETECTION OF INDIVIDUAL NUCLEOTIDE POLYMORPHISMS
EP1176212A1 (en) * 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
WO2002008462A1 (en) * 2000-07-24 2002-01-31 Consortium National De Recherche En Genomique (Cnrg) Method for haplotyping by mass spectrometry
US9416424B2 (en) 2001-03-02 2016-08-16 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US8815513B2 (en) 2001-03-02 2014-08-26 Ibis Biosciences, Inc. Method for rapid detection and identification of bioagents in epidemiological and forensic investigations
US8265878B2 (en) 2001-03-02 2012-09-11 Ibis Bioscience, Inc. Method for rapid detection and identification of bioagents
US8214154B2 (en) 2001-03-02 2012-07-03 Ibis Biosciences, Inc. Systems for rapid identification of pathogens in humans and animals
US9752184B2 (en) 2001-03-02 2017-09-05 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US8017358B2 (en) 2001-03-02 2011-09-13 Ibis Biosciences, Inc. Method for rapid detection and identification of bioagents
US8268565B2 (en) 2001-03-02 2012-09-18 Ibis Biosciences, Inc. Methods for identifying bioagents
US8563250B2 (en) 2001-03-02 2013-10-22 Ibis Biosciences, Inc. Methods for identifying bioagents
US8802372B2 (en) 2001-03-02 2014-08-12 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US8921047B2 (en) 2001-06-26 2014-12-30 Ibis Biosciences, Inc. Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US8380442B2 (en) 2001-06-26 2013-02-19 Ibis Bioscience, Inc. Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby
US8298760B2 (en) 2001-06-26 2012-10-30 Ibis Bioscience, Inc. Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby
WO2004009849A1 (en) * 2002-07-19 2004-01-29 Isis Pharmaceuticals, Inc. Methods for mass spectrometry analysis utilizing an integrated microfluidics sample platform
US8822156B2 (en) 2002-12-06 2014-09-02 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US9725771B2 (en) 2002-12-06 2017-08-08 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8476415B2 (en) 2003-05-13 2013-07-02 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US8013142B2 (en) 2003-09-11 2011-09-06 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7956175B2 (en) 2003-09-11 2011-06-07 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US9447462B2 (en) 2004-02-18 2016-09-20 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8187814B2 (en) 2004-02-18 2012-05-29 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US8987660B2 (en) 2004-05-24 2015-03-24 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US9449802B2 (en) 2004-05-24 2016-09-20 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US8173957B2 (en) 2004-05-24 2012-05-08 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US8407010B2 (en) 2004-05-25 2013-03-26 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA
US9873906B2 (en) 2004-07-14 2018-01-23 Ibis Biosciences, Inc. Methods for repairing degraded DNA
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US8551738B2 (en) 2005-07-21 2013-10-08 Ibis Biosciences, Inc. Systems and methods for rapid identification of nucleic acid variants
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
US8871471B2 (en) 2007-02-23 2014-10-28 Ibis Biosciences, Inc. Methods for rapid forensic DNA analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US20110137022A1 (en) * 2008-08-20 2011-06-09 Dennis Paul Michaud Method for the analysis of oligonucleotides
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8609430B2 (en) 2008-09-16 2013-12-17 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8252599B2 (en) 2008-09-16 2012-08-28 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US9027730B2 (en) 2008-09-16 2015-05-12 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US9023655B2 (en) 2008-09-16 2015-05-05 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US9165740B2 (en) 2009-02-12 2015-10-20 Ibis Biosciences, Inc. Ionization probe assemblies
US8796617B2 (en) 2009-02-12 2014-08-05 Ibis Biosciences, Inc. Ionization probe assemblies
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
CN113881810A (zh) * 2021-11-02 2022-01-04 南方科技大学 一种新型冠状病毒病原微生物检测方法

Also Published As

Publication number Publication date
JP2000512497A (ja) 2000-09-26
CA2257866A1 (en) 1997-12-18
EP0954611A1 (en) 1999-11-10

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