WO1997044281A1 - Biological purification of septic tanks by extender effect - Google Patents

Biological purification of septic tanks by extender effect Download PDF

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Publication number
WO1997044281A1
WO1997044281A1 PCT/BE1996/000053 BE9600053W WO9744281A1 WO 1997044281 A1 WO1997044281 A1 WO 1997044281A1 BE 9600053 W BE9600053 W BE 9600053W WO 9744281 A1 WO9744281 A1 WO 9744281A1
Authority
WO
WIPO (PCT)
Prior art keywords
effect
additive
bio
septic tanks
purification system
Prior art date
Application number
PCT/BE1996/000053
Other languages
French (fr)
Inventor
Guy Knapen
Original Assignee
Bevil S.P.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bevil S.P.R.L. filed Critical Bevil S.P.R.L.
Priority to AU59930/96A priority Critical patent/AU5993096A/en
Priority to PCT/BE1996/000053 priority patent/WO1997044281A1/en
Publication of WO1997044281A1 publication Critical patent/WO1997044281A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used

Definitions

  • the biological preparation developed by the firm BEVIL sa allows to continuously inoculate, for a minimum period of 6 months, a sceptual pit with a mixture of spores of non-pathogenic bacteria of the genus "Bacillus" in order to speed up the processes biological degradations of biodegradable organic matter contained in household effluents
  • This preparation is composed of 4 elements all perfectly compatible with the requirements of biodegradability linked to use in the bioepura ⁇ on sector and in accordance with the directives promulgated by the European Commission under numbers 219/90 and 220/90
  • the first element is a mixture of spores produced by 4 different Bacillus species (poiymixa, megate ⁇ um, psychrophilus and pumilus) characterized by their capacity to hydrolyze biopolymers (starch, protein, lipids and cellulose) under temperature conditions between 10 and 45 ° celsius.
  • the second element is a natural polymer of manuronic and guluronic acids approved as a food additive by the FDA (Food and Drug Admirustrauon of the USA) with the particularity of forming a three-dimensional matnec in the presence of polyvalent cations (calcium for example)
  • the third element is a cellulose-laden derivative, carboxymethylcellulose (CMC) which is added as a filler in the manufacture of the material
  • the fourth element (Compound D) consists of a charge in / yma ⁇ quc (amylasc, proteasc and cellulase) which accelerates the germination of spores in the pit
  • This preparation is in the form of dry granules with a relative humidity of less than 7% allowing long-term storage (> 3 years)
  • Bacillus strains Bacillus poiymixa, Bacillus psychrophilus, Bacillus megaterium and Bacillus puminlus
  • Bacillus poiymixa Bacillus poiymixa, Bacillus psychrophilus, Bacillus megaterium and Bacillus puminlus
  • a growth medium comprising yeast extract, peptones, nut ⁇ ent broth (Difco) and mineral salts. Sporulation is complete after 48 to 72 hours of growth, the spores are harvested by continuous cent ⁇ fugation of the cultures.
  • the spores are suspended in a preparation allowing their drying by atomization and obtaining a powder with 10 n spores per gram.
  • Carboxymethyl cellulose (CMC) is marketed by hrme SIGMA Chemical under the label Carboxymethyl cellulose-Sodium sali (Low viscosity)
  • the enzymatic activities (amylase, protease and cellulase), provided by specialized firms are adsorbed on a preparation of chaigé cellulose Compounds A, B, C and D are mixed in the liquid phase at the desired concentrations and then extruded into a solution of Calcium chloride to obtain beads with an average diameter of 2 mm. This material is then dried to a residual relative humidity of less than 7%. The dried preparation is then used as a raw material for the formulation of commercial products.

Abstract

The invention features the preparation of a dry Bacillus spore premix with extender effect to facilitate the long term continuous treatment of domestic effluents.

Description

DESCRIPTION DESCRIPTION
« Bioépuration des fosses septiques par effet retard »"Bio-purification of septic tanks by delay effect"
La préparation biologique mise au point par la firme BEVIL sa permet d'inoculer en continu, pendant une période minimale de 6 mois, une fosse scepUque par un mélange de spores de bactéπes non-pathogènes du genre « Bacillus » afin d'accélérer les processus biologiques de dégradations des matières organiques biodégradables contenues dans les effluents ménagersThe biological preparation developed by the firm BEVIL sa allows to continuously inoculate, for a minimum period of 6 months, a sceptual pit with a mixture of spores of non-pathogenic bacteria of the genus "Bacillus" in order to speed up the processes biological degradations of biodegradable organic matter contained in household effluents
Cette préparation est composée de 4 éléments tous parfaitement compatibles avec les impératifs de biodégradabihté liés a une utilisation dans le secteur de la bioépuraϋon et en conformité avec les directives promulguées par la Commission Européenne sous les numéros 219/90 et 220/90This preparation is composed of 4 elements all perfectly compatible with the requirements of biodegradability linked to use in the bioepuraϋon sector and in accordance with the directives promulgated by the European Commission under numbers 219/90 and 220/90
Le premier élément (Composé A) est un mélange de spores produites par 4 espèces différentes de Bacillus (poiymixa, mégateπum, psychrophilus et pumilus) caractéπsées par leur capacité à hydrolyscr des biopolymères (amidon, protéine, lipides et cellulose) dans des conditions de température comprises entre 10 et 45° celsius.The first element (Compound A) is a mixture of spores produced by 4 different Bacillus species (poiymixa, megateπum, psychrophilus and pumilus) characterized by their capacity to hydrolyze biopolymers (starch, protein, lipids and cellulose) under temperature conditions between 10 and 45 ° celsius.
Le second élément (Composé B) est un polymère naturel d'acides manuronique et guluronique agréé comme additif alimentaire par le FDA (Food and Drug Admirustrauon des USA ) présentant la particularité de former une matnec tridimensionnelle en présence de cations polyvalents (calcium par exemple)The second element (Compound B) is a natural polymer of manuronic and guluronic acids approved as a food additive by the FDA (Food and Drug Admirustrauon of the USA) with the particularity of forming a three-dimensional matnec in the presence of polyvalent cations (calcium for example)
Le troisième élément (Composé C) est un dérivé chargé de la cellulose , le carboxyméthyl- cellulose (CMC) qui est ajoutée en temps que charge dans la fabrication du matérielThe third element (Compound C) is a cellulose-laden derivative, carboxymethylcellulose (CMC) which is added as a filler in the manufacture of the material
Le quatrième élément (Composé D) est constitué d'une charge en/ymaϋquc (amylasc, protéasc et cellulase) qui permet d'accélérer la germination des spores dans la fosseThe fourth element (Compound D) consists of a charge in / ymaϋquc (amylasc, proteasc and cellulase) which accelerates the germination of spores in the pit
Cette préparation se présente sous la forme de granulés sec piésentant une humidité relative inférieure à 7% permettant un stockage à long terme (> 3 ans)This preparation is in the form of dry granules with a relative humidity of less than 7% allowing long-term storage (> 3 years)
Ces granulés peuvent ensuite être incorporés à des préparations sèches permettant une manipulaUon aisée du matériel biologique préparé soit sous la forme de poudres hétérogènes, de comprimés ou de toutes autres formes selon les besoins commerciaux. La concentration des préparations en spores de Bacillus est établie dans le but d'assurer un apport continuel de 100.000 spores par 24 heures et par gramme de préparationThese granules can then be incorporated into dry preparations allowing easy handling of the biological material prepared either in the form of heterogeneous powders, tablets or any other form according to commercial needs. The concentration of Bacillus spore preparations is established in order to ensure a continuous supply of 100,000 spores per 24 hours and per gram of preparation
Composé ACompound A
Les souches de Bacillus suivantes (Bacillus poiymixa, Bacillus psychrophilus, Bacillus megaterium et Bacillus puminlus ) sont propagées en culture immergée dans un milieu de croissance comprenant du yeast extract, des peptones, du nutπent broth (Difco) et des sels minéraux. La sporulation est complète après 48 à 72 heures de croissance, les spores sont récoltées par centπfugation continue des cultures. Dans un deuxième temps les spores sont suspendues dans une préparation permettant leur séchage par atomisation et l'obtention d'une poudre à 10n spores par gramme.The following Bacillus strains (Bacillus poiymixa, Bacillus psychrophilus, Bacillus megaterium and Bacillus puminlus) are propagated in culture immersed in a growth medium comprising yeast extract, peptones, nutπent broth (Difco) and mineral salts. Sporulation is complete after 48 to 72 hours of growth, the spores are harvested by continuous centπfugation of the cultures. In a second step, the spores are suspended in a preparation allowing their drying by atomization and obtaining a powder with 10 n spores per gram.
Composé BCompound B
L'alginate de sodium utilisé est commercialisé par la firme PROTAN sous le label PROTANALThe sodium alginate used is marketed by the firm PROTAN under the label PROTANAL
SF- 120SF- 120
Composé CCompound C
La carboxyméthy] cellulose (CMC) est commercialisée par la hrme SIGMA Chemical sous le label Carboxyméthyl cellulose-Sodium sali (Low viscosity)Carboxymethyl cellulose (CMC) is marketed by hrme SIGMA Chemical under the label Carboxymethyl cellulose-Sodium sali (Low viscosity)
Composé D • Compound D •
Les activités enzymatiques (amylase, protease et cellulase), f ournies par les firmes spécialisées sont adsorbées sur une préparation de cellulose chaigée Les composés A,B,C et D sont mélangés en phase liquide aux concentrations désirées puis cxtrudés dans une solution de chlorure de Calcium pour obtenir des billes d'un diamètre moyen de 2 mm. Ce matériel est ensuite séché jusqu'à une humidité relative résiduelle inférieure à 7% . La préparation séchéc sert ensuite de matière première pour la formulation des produits commerciaux. The enzymatic activities (amylase, protease and cellulase), provided by specialized firms are adsorbed on a preparation of chaigé cellulose Compounds A, B, C and D are mixed in the liquid phase at the desired concentrations and then extruded into a solution of Calcium chloride to obtain beads with an average diameter of 2 mm. This material is then dried to a residual relative humidity of less than 7%. The dried preparation is then used as a raw material for the formulation of commercial products.

Claims

REVENDICATIONS
1° Pπmauté comme additif à effet retard pour un système de bioépuration des fosses septiques.1 ° Pπmauté as a delay effect additive for a bio-purification system for septic tanks.
2° Application simple du produit sec dans des formulations diverses.2 ° Simple application of the dry product in various formulations.
3° L'ensemble des composants du produit sont parfaitement biodégradables3 ° All the components of the product are perfectly biodegradable
4° Produit biologique fourni complet pour formulation.4 ° Biological product supplied complete for formulation.
5° Effet revendiqué effectif dans la gamme de température de 10 à 50°C. f? Effet revediqué de l'effet retard pendant six mois dans les fosses. 5 ° Effect claimed effective in the temperature range from 10 to 50 ° C. f? Revedicated effect of the delay effect for six months in the pits.
REVENDICATIONS MODIFIEESAMENDED CLAIMS
[reçues par le Bureau International le 18 mars 1997 (18.03.97); revendications 1-6 remplacées par les nouvelles revendications 1-4 (1 page)][received by the International Bureau on March 18, 1997 (March 18, 1997); claims 1-6 replaced by new claims 1-4 (1 page)]
Additif à effet retard pour système de bioépuration des fosses septiques. Cet additif est composé de granulés d'alginate de calcium encapsulant de la carboxyméthylcellulose, des micro-organismes sous forme non-végétative et certaines préparations enzymatiques spécifiques au milieu à traiter. Additif à effet retard pour un système de bioépuration des fosses septiques suivant la revendication n° 1 caractérisé par une biodégradabilité des granulés étalée sur une période de 6 mois permettant une inoculation continue de la fosse septique à partir d'une application unique. La fosse septique est ainsi inoculée en permanence par les micro-organismes sous forme non-végétative. Ceux-ci se transforment en cellule végétative au contact de la matière à traiter qui contient les éléments nécessaires au développement bactérien. Additif à effet retard pour un système de bioépuration des fosses septiques suivant la revendication n°1 avec un effet revendiqué dans la gamme de 10°C à 50°C.Additive with delay effect for bio-purification system of septic tanks. This additive is composed of calcium alginate granules encapsulating carboxymethylcellulose, microorganisms in non-vegetative form and certain enzymatic preparations specific to the medium to be treated. Additive with a delay effect for a bio-purification system for septic tanks according to claim 1, characterized by a biodegradability of the granules spread over a period of 6 months allowing continuous inoculation of the septic tank from a single application. The septic tank is thus permanently inoculated by microorganisms in non-vegetative form. These transform into a vegetative cell on contact with the material to be treated which contains the elements necessary for bacterial development. Additive with delay effect for a bio-purification system for septic tanks according to claim 1 with a claimed effect in the range from 10 ° C to 50 ° C.
Additif à effet retard pour un système de bioépuration des fosses septiques suivant la revendication n°1 avec un effet revendiqué après la contamination de la fosse par des antibiotiques ou des produits chimiques grâce au dépôt des granulés dans le fond de la fosse où l'action de ces produits est considérée comme négligeable, mais surtout par l'action de réinoculation continue autonome de la fosse jusqu'à neutralisation complète de l'effet poison. Additive with delay effect for a bio-purification system for septic tanks according to claim 1 with a claimed effect after contamination of the tank by antibiotics or chemicals thanks to the deposition of granules in the bottom of the tank where the action of these products is considered negligible, but above all by the action of autonomous continuous reinoculation of the pit until complete neutralization of the poison effect.
PCT/BE1996/000053 1996-05-21 1996-05-21 Biological purification of septic tanks by extender effect WO1997044281A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU59930/96A AU5993096A (en) 1996-05-21 1996-05-21 Biological purification of septic tanks by extender effect
PCT/BE1996/000053 WO1997044281A1 (en) 1996-05-21 1996-05-21 Biological purification of septic tanks by extender effect

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/BE1996/000053 WO1997044281A1 (en) 1996-05-21 1996-05-21 Biological purification of septic tanks by extender effect

Publications (1)

Publication Number Publication Date
WO1997044281A1 true WO1997044281A1 (en) 1997-11-27

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Application Number Title Priority Date Filing Date
PCT/BE1996/000053 WO1997044281A1 (en) 1996-05-21 1996-05-21 Biological purification of septic tanks by extender effect

Country Status (2)

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AU (1) AU5993096A (en)
WO (1) WO1997044281A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2831080A1 (en) * 2001-10-19 2003-04-25 Bevil Sprl Production of microspheres for wastewater treatment, comprises gelling a mixture of sodium alginate and carboxymethyl cellulose solutions containing bacteria in a bath containing enzymes
EP2407433A1 (en) * 2010-07-15 2012-01-18 Da Volterra Methods for the inactivation of antibiotics

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2482130A1 (en) * 1980-05-12 1981-11-13 Lumer Ste Indle Produits Chimi Inoculum for septic tanks and purificn. digesters - prepd. by drying bacterial cultures with molecular sieve, then adding enzyme and dispersant
EP0077856A1 (en) * 1981-10-27 1983-05-04 Societe Industrielle Des Produits Chimiques Lumer Process for the preparation of an inoculum for the maintenance of septic tanks and the like, comprising dehydrated microorganisms and enzymes, and inoculums prepared by this process
FR2699525A1 (en) * 1992-12-22 1994-06-24 Hecke Jean Claude Van Den Compsn. for purifying contaminated aq. liquids pref. water
WO1995015924A1 (en) * 1993-12-09 1995-06-15 Reckitt & Colman Inc. Composition and method for sewage treatment using fungal and bacterial enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2482130A1 (en) * 1980-05-12 1981-11-13 Lumer Ste Indle Produits Chimi Inoculum for septic tanks and purificn. digesters - prepd. by drying bacterial cultures with molecular sieve, then adding enzyme and dispersant
EP0077856A1 (en) * 1981-10-27 1983-05-04 Societe Industrielle Des Produits Chimiques Lumer Process for the preparation of an inoculum for the maintenance of septic tanks and the like, comprising dehydrated microorganisms and enzymes, and inoculums prepared by this process
FR2699525A1 (en) * 1992-12-22 1994-06-24 Hecke Jean Claude Van Den Compsn. for purifying contaminated aq. liquids pref. water
WO1995015924A1 (en) * 1993-12-09 1995-06-15 Reckitt & Colman Inc. Composition and method for sewage treatment using fungal and bacterial enzymes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2831080A1 (en) * 2001-10-19 2003-04-25 Bevil Sprl Production of microspheres for wastewater treatment, comprises gelling a mixture of sodium alginate and carboxymethyl cellulose solutions containing bacteria in a bath containing enzymes
EP2407433A1 (en) * 2010-07-15 2012-01-18 Da Volterra Methods for the inactivation of antibiotics
WO2012007536A1 (en) * 2010-07-15 2012-01-19 Da Volterra Methods for the inactivation of antibiotics

Also Published As

Publication number Publication date
AU5993096A (en) 1997-12-09

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