WO1997040194A1 - Procede ameliore pour un pretraitement biologique de copeaux de bois - Google Patents

Procede ameliore pour un pretraitement biologique de copeaux de bois Download PDF

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Publication number
WO1997040194A1
WO1997040194A1 PCT/US1997/006974 US9706974W WO9740194A1 WO 1997040194 A1 WO1997040194 A1 WO 1997040194A1 US 9706974 W US9706974 W US 9706974W WO 9740194 A1 WO9740194 A1 WO 9740194A1
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Prior art keywords
wood
medium
biological agent
altering
enzymes
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PCT/US1997/006974
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English (en)
Inventor
Spencer W. Eachus
Bryan J. Kaphammer
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Union Camp Corporation
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Publication of WO1997040194A1 publication Critical patent/WO1997040194A1/fr

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    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms

Definitions

  • the present invention relates to a method for the treatment of wood pulp and other wood materials, and more particularly using fungi, culture products thereof such as enzymes, or substances therefrom to inoculate compressed substrate.
  • the present invention has particular application in the preparation of wood materials, such as wood pulp.
  • Wood is comprised in large part of cellulose and hemicellulose fiber and amorphous, non-fibrous lignin.
  • the lignin functions to hold the fibrous components together.
  • the wood substrate is converted to a fibrous mass by separating the fiber either chemically or mechanically.
  • Such processes are involved in the preparation of paper and paper products in which, generally, the wood chip starting material is transformed into a fibrous mass of wood pulp during the pulping process.
  • the pulping process may be carried out by a variety of mechanical, chemical or biological methods.
  • the present invention alleviates these problems by accelerating the incubation process, or by eliminating the need for growth of the fungi by application of enzymes, thereby speeding up the production of denatured wood; i.e., conversion of wood chips to wood pulp.
  • This is accomplished through recognition that the rate limiting step in the process of fungal or enzymatic degradation of wood substrate is the time required for the fungal culture to grow through or for the enzyme to penetrate the wood chips to reach sufficient portions of the interior of the wood chips.
  • the biological agents such as fungal or bacterial culture, culture products thereof, such as enzymes, substances produced therefrom, and/or enzymes from non-microbial sources, are delivered into the substrate and are not merely applied to the surface.
  • nutrients, growth stimulators, buffers, surfactants and other promoters are likewise deliverable into the substrate with the biological agent as desired.
  • One advantage of the present invention is that it provides an accelerated, more efficient means of converting wood chips to wood pulp.
  • a further advantage is that the process allows for recovery and recycling of the biological product solution or suspension.
  • Another advantage of the invention is the removal of undesirable resins and other extractives which are expressed during the compression step, separated from the wood chip and thereby removed from subsequent steps of the pulp refining and paper manufacturing process.
  • the present invention comprises a method for altering the structure or composition of wood which method comprises adding to compressed wood a medium comprising a structure- altering or composition-altering effective amount of at least one biological agent which may comprise one or more fiingi or bacteria, one or more culture products thereof, such as enzymes, one or more substances obtained therefrom, one or more enzymes from non- microbial sources, or combinations thereof, to produce inoculated or impregnated wood upon decompression.
  • Compression of the wood substrate prior to application of the biological agent introduces fractures in the wood substrate thereby allowing the biological agent to penetrate the wood structure.
  • the biological agent is drawn into the wood substrate as the wood re-expands during decompression.
  • the biological agent may be drawn into fissures in the wood substrate by capillary action after decompression.
  • the wood comprises wood chips and the method is useful in the production of wood pulp.
  • the wood may be compressed by any suitable means, such as, for example, by means of a compression screw device or a roll press device.
  • the wood is subjected to pressure in the range from about 200 to about 10,000 psig; the pressure may be applied for about 0.1 to about 60 seconds.
  • the wood may be subject to a pretreatment step to remove undesirable microorganisms and other contaminants prior to inoculation with the biological agent. This pretreatment step may occur prior to compressing the wood.
  • the pretreatment step comprises subjecting the wood to steam immediately prior to the compression step.
  • other pretreatment agents may include hot water, dry heat, gamma, ultraviolet radiation, infrared radiation and gas treatment.
  • the biological agent preferably comprises one or more fungi or bacteria, one or more culture products thereof, such as enzymes, one or more substances obtained therefrom, one or more enzymes from non-microbial sources, one or more chemically modified enzymes, or combinations thereof.
  • the fiingi or bacteria utilized in the process of the invention may comprise lipid-degrading, protein-degrading, lignin-degrading, cellulose-degrading, or hemicellulose-degrading fiingi, bacteria or combinations thereof, either as combinations of species or strains of fungi or bacteria or as individual fungal or bacterial species or strains with multiple functionalities.
  • Suitable fungi may be selected from the group consisting of the genera Ceriporiophsis, Phcmerochaete and Ophiostoma.
  • the biological agent may alternatively or additionally comprise culture products produced by fungi or bacteria cultures, or the substances obtained from such culture products, for example enzymes, such as lipolytic, proteolytic, ligninolytic, cellulolytic and hemicellulolytic enzymes, e.g., lipase, protease, cellulase, hemicellulase, ligninase and laccase.
  • enzymes from non-microbial sources such as porcine pancreatic lipase, could be use in place of or in conjunction with microbial cultures or culture products.
  • the inoculating or impregnating medium of biological agent is preferably maintained at a temperature in the range of about 10° to about 100°C, and a pH in the range from about 2 to about 11.
  • the medium may further comprise additional compounds suitable as nutrients or growth stimulators for the fungi or bacteria, as buffers, salts, or co-factors for the enzymes, or as dispersion agents to promote distribution of the fungi or other biological agent throughout the wood.
  • the wood chips or other wood substrate material is contacted with excess medium for about 5 to about 60 seconds. The excess medium is then removed from the wood chips and either discarded or reused in further inoculation of wood chips.
  • the wood chips or other wood substrate material is contacted with excess medium containing the biological agent for extended periods of time from 60 seconds to two weeks.
  • the excess medium is then removed from the wood chips and either discarded or reused in further inoculation of wood chips.
  • the inoculated wood chips are incubated for a sufficient period of time to alter the structure or composition of the wood to the desired state; preferably the incubation period lasts for about 60 seconds to about 14 days.
  • the duration may be adjusted to accomplish a desired degree of decomposition of lipid, protein, lignin, cellulose or hemicellulose, or combinations thereof.
  • enzymes when enzymes are used, there may be no measurable removal or decomposition of lignin, cellulose or hemicellulose, or combinations thereof; rather, only a small portion of the covalent bonds of these wood constituents may be hydrolyzed.
  • the present invention comprises a method for altering the structure or composition of wood material which method comprises adding to compressed substrate, e.g., wood chips, a medium comprising a structure-altering or composition-altering effective amount of at least one biological agent comprising one or more fungi or bacteria, one or more culture products thereof, such as enzymes, one or more substances obtained therefrom, one or more enzymes from non-microbial sources, or combinations thereof, to produce inoculated or impregnated substrate upon decompression.
  • Compression of the substrate prior to application of the biological agent introduces fractures and fissures in the substrate thereby allowing the biological agent to penetrate the structure of the substrate.
  • Biological agent is drawn into the substrate as the substrate re-expands during decompression as well as by capillary action. If the biological agent is contacted with partially or fully decompressed substrate, the biological agent will, to a lesser or greater extent, be drawn into the substrate through the fractures and fissures by means of capillary action.
  • the process of the present invention will be described primarily in the context of converting wood chips to wood pulp for use, for example, in the paper manufacturing industry. However, it is recognized and understood that the process of the present invention is applicable as well to a wide variety of applications in which biological agents, including, but not limited to, fiingi and bacteria, or their culture products or substances obtained therefrom, such as enzymes, or enzymes from non-microbial sources, are the active agents in the desirable degradation of wood materials. Likewise, the process of the present invention will be described primarily with regard to use of biological agent comprising fungi or bacteria, culture products thereof, and substances obtained therefrom, such as enzymes, or enzymes from non- microbial sources, or combinations thereof
  • the process of the present invention provides a method for altering the composition and structure of wood chips and other wood material substrates using a medium comprising one or more biological agent which agent comprises one or more naturally-occurring or bioengineered strain of fungi or bacterial, culture products of such fungi or bacteria, or active substances produced in and extracted from such biological cultures, such as enzymes, or enzymes from non-microbial sources, or combinations thereof.
  • the medium comprising biological agent is applied to the wood material substrate, for example wood chips, after the substrate has been compressed to a sufficient degree to introduce fractures and microfissures or the like in the substrate material.
  • the substrate is decompressed during or after inoculation or impregnation of the substrate with biological agent.
  • the inoculation or impregnation step occurs after decompression of the substrate and the medium comprising biological agent is drawn in by capillary action to the fractures and microfissures introduced during the compression step. Capillary action will occur in the inoculation/impregnation process in the former embodiment as well.
  • the inoculated or impregnated substrate is incubated for a sufficient period of time for the biological agent to effect the desired degree of degradation of the substrate's composition and/or structure.
  • stimulation and “impregnation” are used to refer to contacting substrate with living biological agents and non-living compositions respectively; e.g., fungi cultures and enzyme solutions, respectively.
  • the terms are otherwise interchangeable when describing the process of the invention.
  • Compression of the wood substrate to form compressed wood may be accomplished by any suitable means known or developed in the art. Compression of the wood is intended to provide a substrate which, after addition of the biological agent and when allowed to decompress and/or by capillary action, will draw the medium comprising biological agent into the interior of the wood, thereby enhancing distribution of the active biological agent throughout the wood substrate.
  • the degree of compression is sufficient to disrupt the structure of the wood to introduce fractures or microfissures in the wood. Such fractures or microfissures serve to further enable distribution of the biological agent uniformly throughout the wood.
  • the wood is subject to pressure in the range of 200 to 10,000 psig; preferably 400 to 8,000 psig.
  • the pressure is applied for a sufficient time to compress the wood and create the desired fissures and fractures in the wood as described previously; typically, at least about 0.1 seconds, preferably 0.1 to 60 seconds.
  • the pressure employed is sufficient to induce cracks and fissures in the wood chips and may collapse airspaces in the wood chip and the individual fibers. This allows for direct access of the impregnation solution into the chips and fibers after release of the pressure and during the resulting mechanical expansion of the chips and fibers.
  • the impregnation solution is drawn into the voids created in the inner chip and fiber structure upon release of the pressure.
  • the fissures may also serve to draw in the impregnation solution by capillary action.
  • the maximum pressure employed is adequate to produce the results described above, but not such as to collapse the entire structure of the wood chips and damage the fibers. Determination of suitable compression pressures for various wood chip substrates is within the ability of the skilled practitioner in the art.
  • the wood chip substrate may be subjected to pressure, and hence compressed, by means of a roll press device or a screw compression device.
  • An example of the latter is the lMPRESSIFlNER® compression device which is currently used in the industry to press wood chips to affect pulp quality and which may reach pressures in excess of 6000 psig using a 4: 1 compression ratio.
  • the lMPRESSIFlNER apparatus is particularly suitable for use in the process of the present invention because it is readily adaptable for application of the biological agent to the compressed wood chips as they are discharged from the barrel of the device.
  • the medium comprising biological agent may be added to the compressed wood chips in lieu of the water or chemical solutions which may typically be applied at that point in the pulping process. See, Leask, R.A., "Groundwood - Chip", pp.190-119, in Britt, K.W., Ed., Handbook of Pulp and Paper Technology. 2d ed., Van Nostrand Reinhold Company, New York (1970).
  • the process of the present invention is not limited to any particular compression device; the practitioner in the art may employ any suitable means for subjecting the wood substrate to pressure.
  • the compressed substrate is relaxed to produce fully or partially decompressed wood prior to contacting with medium comprising biological agent.
  • the medium is drawn into the substrate by means of capillary action into the fractures and microfissures in the substrate.
  • the medium comprising biological agent may comprise a solution or suspension of one or more strain of fungi or bacteria, culture products of such fungi or bacteria, such as enzymes, active substances produced in and extracted from such fungal or bacterial cultures, enzymes from non-microbial sources, or combinations thereof.
  • the medium is preferably maintained at a temperature in the range of 10° to 100°C and at a pH in the range of 2 to 11. Selection of suitable temperature and pH within those ranges depends in rum on the selection of biological agent used in the medium; such determination is within the ability of the skilled practitioner.
  • the compression step in the process of the invention provides another advantage of the present invention over the prior art.
  • resins and other extractives may be expressed from the wood substrate. These compounds are undesirable in the pulping process and are separated from the wood chips prior to the inoculation step according to the process of the invention. They are, thereby, removed from subsequent steps of the pulp refining and paper manufacturing process.
  • the wood is preferably contacted with the medium for sufficient time and in the presence of sufficient quantity of biological agent, such that a structure-altering and/or composition-altering effective amount of biological agent is added to the wood; i.e. is absorbed into the wood substrate at least in part by being drawn into the fractures and microfissures in wood as the wood decompresses or by capillary action.
  • the compressed wood is contacted with the medium of biological agent for at least about 5 seconds, more preferably about 5 to about 60 seconds. This forms the inoculated wood.
  • the biological agent is preferably added in excess. This may be accomplished, for example, by adding the compressed wood or the decompressed wood to a solution of biological agent or by pouring or spraying copious amounts of biological agent on to the compressed wood.
  • the compressed wood chips may be delivered from the compression device into a vat or similar container which contains, or to which is subsequently added, the medium comprising active biological agent.
  • the inoculated wood is incubated to convert the wood to wood pulp.
  • an excess of a structure-altering and/or composition-altering effective amount of biological agent is preferably used.
  • the excess of biological agent is separated from the inoculated wood at the beginning of, at the end of, or during, the incubation period. This may be accomplished by removing the inoculated wood from the solution of biological agent, or by draining and collecting the biological agent from the inoculated wood. In either embodiment, the recovered excess biological agent may be discarded or recycled for subsequent use in the process of the invention.
  • the wood is treated prior to the addition of the biological agent to remove, destroy or disable unwanted microorganisms and other non- biologjcal agents and contaminates which be present in or on the wood.
  • This pretreatment step may be performed before or during the compression step.
  • the pretreatment step comprises subjecting the wood substrate to steam; preferably low pressure steam, approximately 60 psig, applied for about 1 to 2 minutes at atmospheric pressure.
  • Other agents may be used in the pretreatment step which have the function of removing, destroying or disabling such microorganisms and other non-biological agents and contaminants; provided, however, that care should be taken to ensure that any such pretreatment agents will not interfere with the subsequent biological agent treatment process or the later pulp refining and paper manufacturing processes.
  • Such alternative agents may include hot water, dry heat, gamma, ultraviolet and infrared radiation, and gas-treatment, for example with ethylene oxide.
  • the inoculated wood is then incubated for sufficient time and under suitable conditions to allow for the desired structural and/or compositional alterations in the wood to occur.
  • the incubation period is about 60 seconds to about 14 days; more preferably 1 to 10 days; still more preferably 1 to 7 days.
  • the biological agent comprises enzymes
  • the precise time, temperature and other parameters of the incubation period will vary depending on the nature of the biological agent used in the process of the invention.
  • the incubation parameters will also vary depending on the nature and degree of structure and composition alteration desired; i.e., the final percentages of lignin, cellulose, hemicellulose and/or resins in the wood to be degraded or the consistency of the pulp desired. Determination of the desired parameters are within the ability of the skilled practitioner in the art.
  • the medium of biological agent comprises a solution or suspension comprising one or more strain of fungi or bacteria, culture products from such fungi or bacteria, such as enzymes, substances obtained from such cultures, enzymes from non-microbial sources, or combinations thereof.
  • the fungi or bacteria suitable for use in the process of the present invention may be any now known or subsequently discovered in nature or bioengineered which have activity in the degradation or alteration of wood material substrates, for example wood chips and the like. As is particularly applicable to the paper manufacturing industry, this includes genera, species and strains of fiingi and bacteria which have lipolytic, proteolytic, ligninolytic, cellulolytic and hemicellulolytic activity. Other genera, species or strains of fungi, bacteria, or other microorganisms with other composition or structure altering activity would be useful in the process of the invention as applied to other wood substrates for conversion to products useful in other applications.
  • Suitable genera of fungi include, by way of example, Ceriporiophsis, Phcmerochaete and Ophiostoma.
  • Other examples of suitable microorganisms for use in the present invention are described in the following publications which are incorporated by reference herein: T. K. Kirk et al., "Enzymatic "Combustion”: The Microbial Degradation of Lignin", Ann.Rev.Microbiol.. 41, 465-505 (1987); L. Jurasek et al., Biological Treatments of Pulps", Biomass. 15, 103-108 (1988); K.-E.
  • the biological agent may comprise culture products and/or substances obtained therefrom, comprising the various enzymes and other substances produced by the fungi or bacteria or resultmg from the interaction and reaction of such enzymes and other substances with components in or added to the culture media
  • These culture products and substances obtained therefrom may comprise, for example, lipolytic, proteolytic, ligninolytic, cellulolytic and hemicellulolytic enzymes, e g , lipase, protease, cellulase, hemicellulase, ligninase and laccase
  • Such enzymes may be present in the medium of biological agent as culture products of the fungal or bacterial culture employed Alternatively or additionally, they may be present therein as a result of extraction from biological culture media or from non-microbial sources In the latter circumstance, they may be added to a solution of fungi or bacteria to augment the naturally produced culture products or they may be used individually or in combination in solution as the "biological agent" in the "medium comprising biological agent".
  • the medium of biological agent used in the process of the invention further comprises additional components to aid in the composition or structure altering process.
  • additional components may include, for example, nutrients, growth stimulators, buffers, surfactants and other promoters which may promote the growth, activity or penetration of the biological agents employed or may improve their distribution throughout the wood substrate Examples of such components would include minerals or sugars as nutrients.
  • such components are delivered into the substrate together with the biological agent as desired
  • the preparation of the medium comprising biological agent is within the ability of the skilled practitioner in the art
  • the nature of the medium, whether aqueous or otherwise, and the concentration or level or activity of biological agent in the medium will varying depending on the biological agent employed and the extent of structural and/or compositional alteration of the substrate desired Again, determination of those parameters is within the ability of the skilled practitioner in the art
  • Mill chipped southern Loblolly pine chips are sprayed with an aqueous suspension of lxl 0 9 fungal spores per 50g of o d chips
  • the fungus employed is a strain of
  • the inoculated chips are made into mechanical pulp by refining first in a Model 418 Andritz Sprout-Bauer primary stage pressurized refiner to 600 ml freeness and then in a Model 401 secondary atmospheric pressure refiner to levels in the range of
  • Example 2 200 o.d. pounds of untreated wood chips from the source in Example 1 are presteamed at atmospheric pressure for 2 minutes using 60 psig steam.
  • the inoculated chips are made into mechanical pulp by refining first in a Model 418 Andritz Sprout-Bauer primary stage pressurized refiner to 600 ml freeness and then in a Model 401 secondary stage atmospheric pressure refiner to three levels in the range of 100 to 200 ml freeness.
  • Example 3 400 o. d. pounds of mill chipped southern loblolly pine chips are presteamed at atmospheric pressure for 2 minutes and separated into four samples of 100 o. d. pounds each. Sample 1 Chips are submerged in 30 liters of lOmM sodium acetate buffer, pH 4.5.
  • Sample 2 Chips are submerged in 30 liters of lOmM sodium acetate buffer, pH 4.5 to which 50,000 units of Clariant Cartazyme HSTM (5 grams at 10,000 units per gram) has been added.
  • Sample 3 Chips are processed through an Andritz Sprout-Bauer Model 560GS lMPRESSIFlNER employing a 4: 1 compression ratio. After the impregnation zone, the destructured compressed chips are allowed to expand into 30 liters of lOmM sodium acetate buffer, pH 4.5. The chips are then submerged in 30 liters of lOmM sodium acetate buffer, pH 4.5.
  • Sample 4 Chips are processed through an Andritz Sprout-Bauer Model 560GS lMPRESSIFlNER employing a 4: 1 compression ratio. After the impregnation zone, the destructured compressed chips are allowed to expand into 30 liters of lOmM sodium acetate buffer, pH 4.5 to which 50,000 units of Clariant Cartazyme HSTM (5 grams at 10,000 units per gram) has been added. The chips are then submerged in 30 liters of lOmM sodium acetate buffer, pH 4.5 containing the same concentration of enzyme.
  • Example 4 400 o. d. pounds of mill chipped southern loblolly pine chips are presteamed at atmospheric pressure for 2 minutes and separated into four samples of 100 o. d. pounds each.
  • Sample 2 Chips are submerged in 30 liters of lOmM sodium phosphate buffer, pH 7.5 to which 50,000 units of Clariant Cartazyme NSTM (50 grams at 1000 units per gram) and 350,000 units of Sigma porcine pancreas Lipase L-3126 (5 grams at 70,000 units per gram) have been added.
  • Sample 3 Chips are processed through an Andritz Sprout-Bauer Model 560GS lMPRESSIFlNER employing a 4:1 compression ratio. After the impregnation zone, the destructured compressed chips are allowed to expand into 30 liters of 1 OmM sodium phosphate buffer, pH 7.5. The chips are then submerged in 30 liters of lOmM sodium phosphate buffer, pH 7.5.
  • Sample 4 Chips are processed through an Andritz Sprout-Bauer Model 560GS lMPRESSIFlNER employing a 4: 1 compression ratio. After the impregnation zone, the destructured compressed chips are allowed to expand into a solution of 1 OmM sodium phosphate buffer, pH 7.5 to which 50,000 units of Clariant Cartazyme NSTM (50 grams at 1000 units per gram) and 350,000 units of Sigma porcine pancreas Lipase L-3126 (5 grams at 70,000 units per gram) have been added. The chips are then submerged in 30 liters of lOmM sodium phosphate buffer, pH 7.5 containing the same concentration of enzymes. All four samples are incubated at 42°C for 48 hours.
  • the liquid is drained from the chips and the chips are made into mechanical pulp by refining first in a Model 418 Andritz Sprout-Bauer primary stage pressurized refiner to 600 ml freeness and then in a Model 401 secondary stage atmospheric pressure refiner to three levels in the range of 100 to 200 ml freeness.

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Abstract

Procédé pour modifier la structure ou la composition du bois, qui consiste à ajouter au bois comprimé un milieu de culture contenant en quantité suffisante pour provoquer une modification de la structure ou de la composition, au moins un mycète ou une bactérie, un ou plusieurs produits de culture de ces derniers, comme par exemple des enzymes, une ou plusieurs substances obtenues avec ces mycètes ou bactéries, une ou plusieurs enzymes provenant de sources non microbiennes, ou des combinaisons de ces diverses substances, pour obtenir du bois inoculé après décompression. La compression du substrat de bois avant ou pendant l'application des agents biologiques provoque dans ce substrat des fractures permettant à l'agent biologique de pénétrer la structure du bois. En outre l'agent biologique est attiré dans le substrat de bois lorsque le bois se redilate au moment de la décompression.
PCT/US1997/006974 1996-04-25 1997-04-25 Procede ameliore pour un pretraitement biologique de copeaux de bois WO1997040194A1 (fr)

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US08/637,397 1996-04-25

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056915A1 (fr) * 2003-12-11 2005-06-23 Valtion Teknillinen Tutkimuskeskus Procede de preparation de pate de bois mecanique
WO2007035481A1 (fr) * 2005-09-16 2007-03-29 Enzymatic Deinking Technologies, L.L.C. Traitement de copeaux de bois au moyen d'enzymes
US8282773B2 (en) * 2007-12-14 2012-10-09 Andritz Inc. Method and system to enhance fiber development by addition of treatment agent during mechanical pulping
US8945347B2 (en) 2004-05-03 2015-02-03 Centre Technique De L'industrie Des Papiers, Cartons Et Celluloses Method for mechanical pulp production
US9127401B2 (en) 2013-01-31 2015-09-08 University Of New Brunswick Wood pulp treatment
US9145640B2 (en) 2013-01-31 2015-09-29 University Of New Brunswick Enzymatic treatment of wood chips
US20210245392A1 (en) * 2020-02-11 2021-08-12 RF Kettle Company, LLC Ethanol-Based Extraction of Soluble Wood Components

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US3486969A (en) * 1965-07-20 1969-12-30 Mo Och Domsjoe Ab Process for the treating of wood chips with fungi to enhance enzymatic hydrolysis of the resinous components
US3962033A (en) * 1973-04-16 1976-06-08 Svenska Traforskningsinstitutet And Skogshogskolan Method for producing cellulose pulp
JPS57143591A (en) * 1981-02-27 1982-09-04 Hokuetsu Paper Mills Treatment of wood chip with high content of alkali soluble extracting component
EP0430915A1 (fr) * 1989-11-27 1991-06-05 Enso-Gutzeit Oy Procédé de fabrication de pâte de bois
US5374555A (en) * 1991-11-26 1994-12-20 The Mead Corporation Protease catalyzed treatments of lignocellulose materials

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3486969A (en) * 1965-07-20 1969-12-30 Mo Och Domsjoe Ab Process for the treating of wood chips with fungi to enhance enzymatic hydrolysis of the resinous components
US3471365A (en) * 1967-08-07 1969-10-07 Asplund Arne J A Apparatus for impregnating fibrous material
US3962033A (en) * 1973-04-16 1976-06-08 Svenska Traforskningsinstitutet And Skogshogskolan Method for producing cellulose pulp
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WO2005056915A1 (fr) * 2003-12-11 2005-06-23 Valtion Teknillinen Tutkimuskeskus Procede de preparation de pate de bois mecanique
US8945347B2 (en) 2004-05-03 2015-02-03 Centre Technique De L'industrie Des Papiers, Cartons Et Celluloses Method for mechanical pulp production
WO2007035481A1 (fr) * 2005-09-16 2007-03-29 Enzymatic Deinking Technologies, L.L.C. Traitement de copeaux de bois au moyen d'enzymes
US8282773B2 (en) * 2007-12-14 2012-10-09 Andritz Inc. Method and system to enhance fiber development by addition of treatment agent during mechanical pulping
US9127401B2 (en) 2013-01-31 2015-09-08 University Of New Brunswick Wood pulp treatment
US9145640B2 (en) 2013-01-31 2015-09-29 University Of New Brunswick Enzymatic treatment of wood chips
US20210245392A1 (en) * 2020-02-11 2021-08-12 RF Kettle Company, LLC Ethanol-Based Extraction of Soluble Wood Components

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