WO2005103370A1 - Traitement enzymatique des pates de bois - Google Patents

Traitement enzymatique des pates de bois Download PDF

Info

Publication number
WO2005103370A1
WO2005103370A1 PCT/US2005/013216 US2005013216W WO2005103370A1 WO 2005103370 A1 WO2005103370 A1 WO 2005103370A1 US 2005013216 W US2005013216 W US 2005013216W WO 2005103370 A1 WO2005103370 A1 WO 2005103370A1
Authority
WO
WIPO (PCT)
Prior art keywords
chips
enzyme
lignin
biopulped
fungus
Prior art date
Application number
PCT/US2005/013216
Other languages
English (en)
Inventor
Gary M. Scott
Thomas E. Amidon
Jeremy Bartholomew
Original Assignee
The Research Foundation Of The State University Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Research Foundation Of The State University Of New York filed Critical The Research Foundation Of The State University Of New York
Publication of WO2005103370A1 publication Critical patent/WO2005103370A1/fr
Priority to US11/412,593 priority Critical patent/US8317975B2/en
Priority to US13/683,642 priority patent/US8668806B2/en
Priority to US14/198,754 priority patent/US8940133B2/en
Priority to US14/603,663 priority patent/US9273431B2/en
Priority to US15/051,742 priority patent/US9683329B2/en
Priority to US15/625,545 priority patent/US9945073B2/en
Priority to US15/927,181 priority patent/US20180245285A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C3/00—Pulping cellulose-containing materials

Definitions

  • the invention relates generally to the field of producing pulp from wood and, more specifically, to a method for producing pulp using biopulping followed by an enzyme treatment.
  • Pulp is the fibrous slurry that is fed to a paper machine to produce paper.
  • Mechanical, chemical and hybrid methods dominate commercial pulping plants. About 25% of worldwide pulp production is mechanical pulp. It is a high-yield process but suffers from high energy costs and damage to the wood fibers. This damage means lower strength paper.
  • These disadvantages cost and quality
  • Biopulping prior to mechanical pulping overcomes the aforementioned disadvantages. The production of pulp begins with wood chips.
  • the wood chips are 'digested' with one or more fungi types prior to mechanical or chemical pulping.
  • the fungi soften the wood chips by degrading or digesting the lignin components of the wood chips.
  • the wood chips are mechanically or chemically pulped into individual fibers.
  • the fungus and the produced enzymes are destroyed during the thermomechamcal pulping process. Due, in large part, to the biochemical action of the fungi, less energy is now required to convert the chips to fibers. Some investigators claim energy savings of at least 30%.
  • the easier conversion from chip to fiber means less damage to the wood fibers.
  • the paper formed from these fibers is stronger.
  • biopulping there are some drawbacks to biopulping, such as a reduction in the brightness and opacity of the resulting fibers.
  • the production of higher quality papers is desirable.
  • Use of biopulped fibers for this application will require improvements in brightness and opacity.
  • Preliminary bleaching studies with hydrogen peroxide and addition of calcium carbonate to improve both brightness and opacity have met with early success.
  • the present invention provides a method for producing pulp that addresses the above and other issues.
  • the present invention provides a process of producing thermomechanical pulp using a combined fungus/enzyme process in which the enzymes are produced in situ.
  • wood chips are treated with a lignin-degrading fungus using the biopulping process.
  • the enzymes that have been produced by the fungus axe extracted from the wood chips in the form of a crude broth.
  • the enzymes are used to treat the pulp prior to completing the refining process.
  • Biopulping has been shown previously to reduce the energy requirements for mechanical pulping.
  • Enzyme treatments have also been shown to be beneficial.
  • a method for processing wood chips includes biopulping the chips, extracting at least one enzyme from the biopulped chips, performing a further pulping of the biopulped chips, and re-introducing the at least one extracted enzyme to the further pulped chips.
  • Fig. 1 illustrates the lignolytic enzyme activity change for the laccase enzyme, where thermomechanical pulping (TMP) is performed over a six hour treatment time on Picea abies (Norway Spruce) wood chips with fungal treatment using P. subserialis, T. versicolor and C. subvermispora, in accordance with the invention
  • Fig. 2 illustrates the lignolytic enzyme activity change for the manganese peroxidase enzyme, for comparison with the results of Fig. 1, in accordance with the invention
  • Fig. 3 illustrates a method for processing wood chips, in accordance with the invention.
  • the invention employs a fungus treatment and a subsequent enzyme treatment to process wood chips.
  • the wood chips are biopulped by inoculating them with a fungal treatment, such as a liquid mixture comprising a lignin-degrading fungus and a corn steep liquor.
  • a fungal treatment such as a liquid mixture comprising a lignin-degrading fungus and a corn steep liquor.
  • Enzymes which are formed as a result of the fungal inoculation are extracted from the wood chips.
  • the enzymes can be extracted as a crude broth or pressate by applying mechanical pressure to the chips following the fungal treatment. A concentrated broth is then formed.
  • a further pulping is performed, such as a first-stage thermomechanical pulping (TMP), to refine the biopulped chips to a coarse pulp.
  • TMP thermomechanical pulping
  • the resulting pulp is subsequently treated with the concentrated enzyme broth.
  • This process has several advantages. First, the enzymes that are re-introduced into the coarse pulp are incubated on the wood and presumably adapted to reacting with the wood. Also, a separate enzyme production process is not necessary since the enzymes that are produced during the biopulping process are utilized. Finally, although mechanical energy is needed to extract the enzymes, the extraction weakens the wood chip structure, which should also reduce the necessary refiner energy in the first stage.
  • the growth of the fungi on the wood chips is a relatively slow process compared to the normal processing time scales in the paper industry. The treatment of the wood chips with the lignin-degrading fungi can take anywhere from two to six weeks or longer depending on the degree of treatment desired.
  • the treatment time can be shortened by using greater concentrations of fungi initially, but this would come at a higher cost.
  • Previous related work has indicated that the inoculation amounts (5g/ton of chips) and treatment time of 2 weeks are reasonably feasible from an economic standpoint.
  • the use of a biological agent should not cause a concern of contamination or other health concerns relating to concentrated cultures of microorganisms since the organisms used are all naturaUy-occurring and limit their attacks to lignocelMosic materials.
  • Picea abies preparation Picea abies was selected as the softwood for this example.
  • different species of woods including hardwoods and/or softwoods, can also be used.
  • the invention can be used with virgin wood or waste wood, including, e.g., kiln dried, air-dried and green wood from industrial, residential, sawmill, construction and demolition sources.
  • logs from a 79- year old tree were debarked with a 36-cm spoke shave, chipped in a Carthage 10-blade chipper, and air dried to approximately 15% moisture by spreading the chips on a tarp.
  • the chips were then screened in a Williams classifier. All fractions were collected and the chips retained on the 15.8, 12.7 and 9.25-mm screens were pooled together and sealed in plastic bags, and stored at room temperature (approximately 24°C) for use throughout this study.
  • TAPPI test method T- 257 cm-97 was followed for all subsequent testing and samples were taken from the pooled material as needed.
  • TAPPI refers to the Technical Association of the Pulp and Paper Industry, Norcross, Georgia.
  • Test Methods consist of a capital T, followed by a space, then a number (assigned sequentially within several Test Method categories), another space, a two-letter designation of classification, a hyphen, and the last two digits of the year published.
  • the reactor was then cooled for approximately two hours until the temperature was below 30°C.
  • the moisture content was brought up to 55% moisture by the addition 200 ml water collected during steaming plus additional distilled makeup water.
  • Fresh fungal inoculum (2.3 ml) and 0.5% (v/v) unsterilized corn steep liquor (CSL) at 50% solids was added to the additional distilled makeup water.
  • the chips can be inoculated with the hgnin-degrading fungus by providing a liquid mixture including the fungal inoculum, and applying the liquid mixture to the chips. The inoculated chips were then incubated under conditions favorable to the propagation of the hgnin-degrading fungus through the chips.
  • the bioreactor was then placed in the incubation chamber at 27°C with forced continuous flow of warm humidified air at a rate of 0.028 cubic meters per minute.
  • House air was measured by a flow meter and humidification was controlled by passing air through two water filled two-liter glass sidearm flasks (in series) through a fritted ground glass sparger. The sidearm flasks were immersed in a 40°C water bath. From the hot water flasks, the warm humidified air passed though a water trap and a final filtering through a 0.2 micron Millipore air filter (for sterilization) before connecting to the individual bioreactors.
  • the TMP produced was sealed in a 40-liter Nalgene® carboy and refrigerated at 4°C until use.
  • Culture Supernatant Purification Purification involved monitoring laccase and manganese peroxidase activity and harvesting the mycelium from P. subserialis (RLG6074-sp), C. subvermispora (L- 14807 SS-3), and T. versicolor (FP-72074) on the first day after peak laccase activity.
  • Mycelium was harvested from the liquid culture by centrifuging for 20 min at 10,000 rpm, followed by treating the crude supernatant with 10% (v/v) acetone and refrigerating for one hour at 4°C to precipitate any extracellular polysaccharide.
  • the broth was centrifuged again for 20 minutes at 10,000 rpm and filtered through a Whatman glass microfiber GF/A 42.5-mm diameter filter.
  • the resulting supernatant was concentrated in a DC-2 ultrafiltration unit (Amicon Corp., Danvers, Mass.) equipped with a 30-kDa molecular weight cutoff hollow fiber filter from an initial volume of 1000 ml to 100 ml.
  • Enzyme activity was monitored at harvest time and after the final concentration.
  • Enzyme Treated TMP First-stage coarse thermomechanical pulp was treated with partially purified culture supernatant from P. subserialis, C. subvermispora, and T.
  • Duplicate reaction vessels contained 2.0 g OD coarse refiner mechanical pulp that was suspended in 5% (w/v) 50-mM sodium acetate buffer (pH 4.5). The pulp in each reaction vessel was mixed with concentrated enzyme broth at a normalized enzyme activity of approximately 1.50 nkatal ml "1 manganese peroxidase. Laccase activity was measured and monitored throughout the experiment. For each fungus, one reaction vessel was setup in duplicate for analysis at 0, 30, 60, 90, 180 and 360-minute intervals in a constant temperature bath of 30°C.
  • Boiling chips were added to the boiling flask with 300 ml of the ethanol-benzene mixture. Samples were extracted for eight hours at brisk boiling with siphoning at approximately ten-minute intervals. After eight hours, the extraction thimbles were removed from the Soxhlet extractors, washed with 100% pure ethanol by placing the thimble in a 100 ml coarse ground glass crucible fitted on a 1000-ml sidearm flask. The thimble was returned to the Soxhlet extractor and extracted for four hours with 100% pure ethanol. The samples were transferred to a Buchner funnel and washed with hot water to remove the ethanol and then allowed to air dry for all subsequent carbohydrate and lignin analyses.
  • Picea abies chips were prepared as previously described, inoculated with Phlebia subserialis, Ceriporiopsis subvermispora, and Trametes versicolor, and incubated for 30 days at 27°C with forced warm humidified air at a rate of 0.028 cubic meters per minute. The chips were thus incubated under conditions favorable to the propagation of the lignin-degrading fungus through the chips.
  • Duplicate 500-g samples were removed from each bioreactor, and double-bagged in 6x9 zip lock bags. One bottom corner of the double bag was cut off with scissors.
  • the stainless steel plates on the top and bottom pressing surfaces of the Williams press (Williams Apparatus Co., Watertown, NY) were cleaned first with soap and water and then dried with ethanol.
  • the press was blocked up at a 45° angle and secured.
  • the zip lock bag containing the sample was placed between the pressing surfaces and a clean 20-dram vial was placed under the cut corner of the bag. Pressure was applied (1500 psi) to the sample and the pressate was captured in the glass vial as a crude broth. Laccase and manganese peroxidase enzyme assays were performed on each vial to determine the enzyme present and enzyme concentration.
  • the enzyme concentration was then adjusted to 1.4 nkatal ml and were used to treat l st -stage TMP as a method to reduce the amount of lignin within the pulp, reducing the electrical refining energy and thereby increasing pulp strength.
  • This system can also be used as a first-stage biobleacbing of mechanical pulp.
  • the enzyme activity levels were monitored, followed by a lignin analysis of the TMP.
  • Table 1 lists the laccase and manganese peroxidase enzyme activity levels throughout the pulp treatment. Initial activity was measured from the concentrated production medium before addition to each sample and then the manganese peroxidase enzyme concentration was normalized to approximately 1.50 nkatal ml "1 for the zero-time condition.
  • Laccase from P. subserialis showed a 22% decrease in activity while T. versicolor and C. subvermispora showed much smaller changes in activity, 3.1 and 1.4%, respectively. This difference may not be significant due to the much lower laccase activity in the enzyme broth from P. subserialis.
  • Initial manganese peroxidase activity levels were on the same order of magnitude for all three fungal extract applications. The range in overall manganese peroxidase activity loss was from 15.8% forR. subserialis to 8.9 and 25.7% loss for T. versicolor and C. subvermispora, respectively.
  • Figures 1 and 2 chart the enzyme activity throughout the experiment and show the decrease in activity over the life of the experiment. In particular, Fig.
  • Fig. 1 illustrates the lignolytic enzyme activity change for the laccase enzyme, where thermomechanical pulping (TMP) is performed over a six hour treatment time on Picea abies (Norway Spruce) wood chips with fungal treatment using P. subserialis, T. versicolor and C. subvermispora.
  • Fig. 2 illustrates the lignolytic enzyme activity change for the manganese peroxidase enzyme, for comparison with the results of Fig. 1.
  • the horizontal axis denotes time, in minutes, from 0 to 400 minutes
  • the left hand vertical axis denotes T.v. and C.s. laccase activity
  • the right hand vertical axis denotes P.s. laccase activity.
  • Fig. 1 illustrates the lignolytic enzyme activity change for the laccase enzyme, where thermomechanical pulping (TMP) is performed over a six hour treatment time on Picea abies (Norway Spruce) wood chips with fungal treatment using P. subse
  • the horizontal axis denotes time, in minutes, from 0 to 400 minutes, while the left hand vertical axis denotes manganese peroxidase activity.
  • Table 2 outlines the results from lignin analysis on the TMP, showing that the lignolytic enzyme treatment from C. subvermispora removed up to 3.66% of the lignin in the sample over a six-hour period, while P. subserialis and T. versicolor reduced the lignin content by similar amounts, 2.35 and 2.67%, respectively. P. subserialis showed a significant decrease in lignin content at the 90-minute sample; however, no significant change occurred after that time interval. Both T. versicolor and C.
  • Table 2 Klason lignin analysis of a Picea abies TMP treated with partially purified enzymes from P. subserialis, T. versicolor and C. subvermispora over 6 hours
  • FIG. 3 illustrates a method for processing wood chips, in accordance with the invention.
  • wood chips are biopulped by inoculating the chips with a lignin-degrading fungus, and incubating.
  • an enzyme is extracted from the biopulped chips as a crade broth and/or pressate.
  • a concentrated broth with the enzyme is prepared.
  • a further mechanical and/or chemical pulping of the chips is performed.
  • the extracted enzyme is re-introduced to the chips by mixing the chips with the concentrated broth.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Paper (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de traitement de copeaux de bois, qui consiste à utiliser un processus combiné de traitement par les champignons et les enzymes dans lequel les enzymes sont produits in situ. Dans une première étape (300), les copeaux de bois sont transformés en pâte biologique par traitement avec un champignon de dégradation de la lignine. Dans une deuxième étape (310), les enzymes produites par le champignon sont extraites des copeaux de bois sous la forme d'un bouillon brut. Les enzymes sont ensuite concentrées dans un mélange liquide (320). Après transformation des copeaux de bois en pâte grossière, par exemple par réduction mécanique (330), le mélange enzymatique concentré est utilisé pour traiter la pâte avant la fin du processus d'affinage (340). Les avantages attendus sont l'économie d'énergie et l'amélioration des propriétés.
PCT/US2005/013216 2004-04-20 2005-04-20 Traitement enzymatique des pates de bois WO2005103370A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US11/412,593 US8317975B2 (en) 2004-04-20 2006-04-27 Product and processes from an integrated forest biorefinery
US13/683,642 US8668806B2 (en) 2004-04-20 2012-11-21 Product and processes from an integrated forest biorefinery
US14/198,754 US8940133B2 (en) 2004-04-20 2014-03-06 Product and processes from an integrated forest biorefinery
US14/603,663 US9273431B2 (en) 2004-04-20 2015-01-23 Product and processes from an integrated forest biorefinery
US15/051,742 US9683329B2 (en) 2004-04-20 2016-02-24 Methods of producing a paper product
US15/625,545 US9945073B2 (en) 2004-04-20 2017-06-16 Methods of producing a paper product
US15/927,181 US20180245285A1 (en) 2004-04-20 2018-03-21 Methods of producing a paper product

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56383704P 2004-04-20 2004-04-20
US60/563,837 2004-04-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/412,593 Continuation-In-Part US8317975B2 (en) 2004-04-20 2006-04-27 Product and processes from an integrated forest biorefinery

Publications (1)

Publication Number Publication Date
WO2005103370A1 true WO2005103370A1 (fr) 2005-11-03

Family

ID=35197025

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/013216 WO2005103370A1 (fr) 2004-04-20 2005-04-20 Traitement enzymatique des pates de bois

Country Status (1)

Country Link
WO (1) WO2005103370A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007035481A1 (fr) * 2005-09-16 2007-03-29 Enzymatic Deinking Technologies, L.L.C. Traitement de copeaux de bois au moyen d'enzymes
US20110073264A1 (en) * 2009-08-13 2011-03-31 The Research Foundation Of State University Of New York Kraft-Pulping of Hot Water Extracted Woodchips
US8317975B2 (en) * 2004-04-20 2012-11-27 The Research Foundation Of The State University Of New York Product and processes from an integrated forest biorefinery
CN103159865A (zh) * 2013-02-01 2013-06-19 北京林业大学 半纤维素及其制备方法
CN111454690A (zh) * 2020-05-21 2020-07-28 南宁雄晋生物科技有限公司 一种利用酶法改性制备木质素基胶黏剂的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5055159A (en) * 1990-05-16 1991-10-08 Wisconsin Alumni Research Foundation Biomechanical pulping with C. subvermispora
US5081027A (en) * 1989-03-16 1992-01-14 Kabushiki Kaisha Kobe Seiko Sho Method for producing pulp by treatment using a microorganism, and its related enzymes
US5620564A (en) * 1994-08-11 1997-04-15 Wisconsin Alumni Research Foundation Method of enhancing biopulping efficacy
US5705383A (en) * 1993-03-19 1998-01-06 Clariant Finance (Bvi) Limited Pitch and lignin degradation with white rot fungi

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5081027A (en) * 1989-03-16 1992-01-14 Kabushiki Kaisha Kobe Seiko Sho Method for producing pulp by treatment using a microorganism, and its related enzymes
US5055159A (en) * 1990-05-16 1991-10-08 Wisconsin Alumni Research Foundation Biomechanical pulping with C. subvermispora
US5705383A (en) * 1993-03-19 1998-01-06 Clariant Finance (Bvi) Limited Pitch and lignin degradation with white rot fungi
US5620564A (en) * 1994-08-11 1997-04-15 Wisconsin Alumni Research Foundation Method of enhancing biopulping efficacy

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8317975B2 (en) * 2004-04-20 2012-11-27 The Research Foundation Of The State University Of New York Product and processes from an integrated forest biorefinery
US8668806B2 (en) 2004-04-20 2014-03-11 The Research Foundation Of The State University Of New York Product and processes from an integrated forest biorefinery
US8940133B2 (en) 2004-04-20 2015-01-27 The Research Foundation For The State University Of New York Product and processes from an integrated forest biorefinery
US9273431B2 (en) 2004-04-20 2016-03-01 The Research Foundation For The State University Of New York Product and processes from an integrated forest biorefinery
US9683329B2 (en) 2004-04-20 2017-06-20 The Research Foundation For The State University Of New York Methods of producing a paper product
US9945073B2 (en) 2004-04-20 2018-04-17 The Research Foundation For The State University Of New York Methods of producing a paper product
WO2007035481A1 (fr) * 2005-09-16 2007-03-29 Enzymatic Deinking Technologies, L.L.C. Traitement de copeaux de bois au moyen d'enzymes
US20110073264A1 (en) * 2009-08-13 2011-03-31 The Research Foundation Of State University Of New York Kraft-Pulping of Hot Water Extracted Woodchips
CN103159865A (zh) * 2013-02-01 2013-06-19 北京林业大学 半纤维素及其制备方法
CN111454690A (zh) * 2020-05-21 2020-07-28 南宁雄晋生物科技有限公司 一种利用酶法改性制备木质素基胶黏剂的方法

Similar Documents

Publication Publication Date Title
US5055159A (en) Biomechanical pulping with C. subvermispora
Worrall et al. Comparison of wood decay among diverse lignicolous fungi
US20170306557A1 (en) Methods of producing a paper product
Kirk et al. Potential applications of bio-ligninolytic systems
Fonseca et al. Biopulping of wood chips with Phlebia brevispora BAFC 633 reduces lignin content and improves pulp quality
CN101597575A (zh) 生物制浆用复合菌种微生物干粉及环保节能型复合菌种生物制浆工艺
US6402887B1 (en) Biopulping industrial wood waste
JPH11505299A (ja) バクテリアで材を処理するための方法
US6958110B2 (en) Apparatus for the production of cellulose paper pulps by biodelignification of vegetative masses
JP4793781B2 (ja) リグノセルロース分解作用を有する白色腐朽菌及びその利用
WO2005103370A1 (fr) Traitement enzymatique des pates de bois
CA1266014A (fr) Blanchiment biologique direct de la pate kraft a l'aide d'un champignon coriolus versicolor
US5460697A (en) Method of pulping wood chips with a fungi using sulfite salt-treated wood chips
CN110453519B (zh) 一种食用菌菌渣的制浆方法
WO2002099183A1 (fr) Procede biomecanique servant a effectuer la reduction en pate d'eucalyptus
CN1291102C (zh) 非木材纤维植物的生物制浆法
WO2002081816A1 (fr) Traitement de copeaux de bois
AU2010201811B2 (en) Isolation and Use of Decay Fungi
Kumar et al. Enzyme cocktail: a greener approach for biobleaching in paper and pulp industry
CN1202944A (zh) 通过用白色腐朽菌预处理来提高化学制浆工艺的效率的方法
WO1997040194A1 (fr) Procede ameliore pour un pretraitement biologique de copeaux de bois
TW200416323A (en) Biopulping method for plant fiber
FI112248B (fi) Uusi valkolahottajasieni ja sen käyttö puun esikäsittelyssä
Mohamad Hasnul et al. Investigation of oil palm empty fruit bunches in biosoda pulping by tropical white-rot fungi, Ganoderma australe (Fr.) Pat.
JP5381119B2 (ja) 樹皮原料から糖類を製造する方法

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 11412593

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

WWP Wipo information: published in national office

Ref document number: 11412593

Country of ref document: US

122 Ep: pct application non-entry in european phase