WO1997034144A1 - Procede permettant de detecter l'effet de substances test a l'aide d'urokinase de poule - Google Patents

Procede permettant de detecter l'effet de substances test a l'aide d'urokinase de poule Download PDF

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Publication number
WO1997034144A1
WO1997034144A1 PCT/EP1997/001219 EP9701219W WO9734144A1 WO 1997034144 A1 WO1997034144 A1 WO 1997034144A1 EP 9701219 W EP9701219 W EP 9701219W WO 9734144 A1 WO9734144 A1 WO 9734144A1
Authority
WO
WIPO (PCT)
Prior art keywords
urokinase
chicken
cells
plasmid
transfected
Prior art date
Application number
PCT/EP1997/001219
Other languages
German (de)
English (en)
Inventor
Gernot Langer
Luisella Toschi
Wolf-Dieter Schleuning
Original Assignee
Schering Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19611780A external-priority patent/DE19611780A1/de
Priority claimed from DE1996154616 external-priority patent/DE19654616A1/de
Application filed by Schering Aktiengesellschaft filed Critical Schering Aktiengesellschaft
Priority to AU22873/97A priority Critical patent/AU2287397A/en
Publication of WO1997034144A1 publication Critical patent/WO1997034144A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

Definitions

  • transfection reagents are, for example, transfection reagents based on liposomal gene transfer.

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé permettant de détecter l'effet de substances test dans des systèmes de culture cellulaire et des substances à l'aide de produits de recombinaison du gène rapporteur du gène urokinase de faible poids moléculaire issu de la poule et de lignées cellulaires recombinées transfectées. L'invention concerne en outre l'utilisation dudit procédé pour analyser des composés et déterminer des récepteurs.
PCT/EP1997/001219 1996-03-14 1997-03-11 Procede permettant de detecter l'effet de substances test a l'aide d'urokinase de poule WO1997034144A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU22873/97A AU2287397A (en) 1996-03-14 1997-03-11 Method of detecting the effect of test substances using hen urokinase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19611780A DE19611780A1 (de) 1996-03-14 1996-03-14 Verfahren zum Nachweis der Wirkung von Testsubstanzen
DE19611780.1 1996-03-14
DE1996154616 DE19654616A1 (de) 1996-12-20 1996-12-20 Verfahren zum Nachweis der Wirkung von Testsubstanzen unter Verwendung von Hühner-Urokinase
DE19654616.8 1996-12-20

Publications (1)

Publication Number Publication Date
WO1997034144A1 true WO1997034144A1 (fr) 1997-09-18

Family

ID=26024119

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/001219 WO1997034144A1 (fr) 1996-03-14 1997-03-11 Procede permettant de detecter l'effet de substances test a l'aide d'urokinase de poule

Country Status (2)

Country Link
AU (1) AU2287397A (fr)
WO (1) WO1997034144A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007488A1 (fr) * 1989-11-16 1991-05-30 The Salk Institute For Biological Studies Elements de reponse de l'acide retinoique beta; compositions et analyses
US5183736A (en) * 1990-03-30 1993-02-02 La Jolla Cancer Research Foundation Methods using estrogen receptor as a constitutive transcriptional activator and a repressor
WO1995006754A1 (fr) * 1993-09-01 1995-03-09 The Regents Of The University Of California Procedes de detection de composes anti-×strogenes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007488A1 (fr) * 1989-11-16 1991-05-30 The Salk Institute For Biological Studies Elements de reponse de l'acide retinoique beta; compositions et analyses
US5183736A (en) * 1990-03-30 1993-02-02 La Jolla Cancer Research Foundation Methods using estrogen receptor as a constitutive transcriptional activator and a repressor
WO1995006754A1 (fr) * 1993-09-01 1995-03-09 The Regents Of The University Of California Procedes de detection de composes anti-×strogenes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHENG, S. M. ET AL: "Tissue plasminogen activator (tPA) as a reporter gene in transient gene expression", GENE (1987), 58(2-3), 299-303 CODEN: GENED6;ISSN: 0378-1119, 1987, XP002034593 *
LANGER, G. ET AL.: "Low-Mr urokinase-type plasminogen activator as a reporter protein", GENE, vol. 161, 1995, pages 287 - 292, XP002034590 *
LESLIE, N.D. ET AL.: "The chicken Urokinase-type Plasminogen Activator gene", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 3, 1990, pages 1339 - 1334, XP002034591 *
STRÄHLE, U. ET AL.: "Synergistic action of the glucocorticoid receptro with transcription factors.", THE EMBO JOURNAL, vol. 7, no. 11, 1988, pages 3389 - 3395, XP002034594 *
YOKOYAMA-KOBAYASHI, MIDORI ET AL: "A signal sequence detection system using secreted protease activity as an indicator", GENE (1995), 163(2), 193-6 CODEN: GENED6;ISSN: 0378-1119, 1995, XP002034592 *

Also Published As

Publication number Publication date
AU2287397A (en) 1997-10-01

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