WO1997034010A1 - Producer of an anti-tumour melanin-containing preparation 'astromelanin' - Google Patents

Producer of an anti-tumour melanin-containing preparation 'astromelanin' Download PDF

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WO1997034010A1
WO1997034010A1 PCT/RU1996/000300 RU9600300W WO9734010A1 WO 1997034010 A1 WO1997034010 A1 WO 1997034010A1 RU 9600300 W RU9600300 W RU 9600300W WO 9734010 A1 WO9734010 A1 WO 9734010A1
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melanin
мел
аче
producer
tumour
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PCT/RU1996/000300
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French (fr)
Russian (ru)
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Svetlana Pavlovna Lyakh
Maxim Lvovich Bulgak
Alexei Grigorievich Isaev
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Svetlana Pavlovna Lyakh
Maxim Lvovich Bulgak
Alexei Grigorievich Isaev
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Priority to AU76572/96A priority Critical patent/AU7657296A/en
Publication of WO1997034010A1 publication Critical patent/WO1997034010A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the invention is subject to biotechnology, in particular, the receipt of biologically active substances in the form of biologically active substances.
  • the properties of the treatment of the treatment of the disease are treated.
  • the resulting melanin-containing product is called “racepatstruo-melanin”.
  • the discovered useful properties of “melting on” - the absence of toxicity and the positive effect - were not known earlier and were not described in the literature.
  • Strain 365 cultivates a well-known method under conditions of aeration and stirring in a liquid mineral medium, for example, the following composition, g / l ⁇ ⁇ ⁇ ⁇ 0.1; Ca ⁇ ⁇ ⁇ 0,1 0,1 0,1 0,00 0,00 0,00 0,0 0,00 0,0 0,00; with glucose (2%) and liquid beer (1%) at 26-28 ° C and ⁇ 3.5 for a week or in a normal culture, for example, for a case.
  • the biomass is separated by the centrifugation and the large amount of washing is carried out by the distilled water, then the biomass is autoclaved
  • a dry melanin-containing substance is a black material with a bright flickering glitter. Its physical and physical properties are consistent with the indicators presented in the above sources.
  • the obtained use is for use P ⁇ luchenny of sh ⁇ amma 365 ⁇ e ⁇ a ⁇ a ⁇ ⁇ azal vys ⁇ uyu a ⁇ - ⁇ ivn ⁇ s ⁇ in ⁇ n ⁇ shenii ⁇ le ⁇ ⁇ a ⁇ a chel ⁇ ve ⁇ a in chas ⁇ n ⁇ s ⁇ i ⁇ le- ⁇ chn ⁇ y line ⁇ a ⁇ tsin ⁇ my ⁇ ls ⁇ y ⁇ ish ⁇ i, ⁇ le ⁇ chny ⁇ lines ⁇ a ⁇ a leg ⁇ g ⁇ and ⁇ a ⁇ zhe ⁇ i treatment b ⁇ lny ⁇ -d ⁇ b ⁇ v ⁇ ltsev.
  • Bely Bog. Very lightly, especially with a 40-10 mg daily dose, recovery - 2 years.
  • Example 2 A large and (doctor) melanoma, 1 ⁇ stage, with a dose of 60–10 mg (daily dose) and place on lymph nodes, a recovery of 1 year 7 months, a high rate of recovery.
  • Strain 365 is not toxic. No adverse events were observed.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The invention pertains to biotechnology, in particular to the production of biologically active substances with anti-tumour properties. Strain 365 of the antarctic black yeast Nadsoniella nigra var. hesuelica is used as a producer of a melanin-containing non-toxic preparation with strong anti-tumour properties.

Description

ПΡΟДУЦΕΗΤ ПΡΟΤИΒΟΟПУΧΟЛΕΒΟГΟ ΜΕЛΑΗИΗСΟДΕΡЖΑЩΕГΟ ПΡΕПΑΡΑΤΑ "ΑСΤΡΟΜΕЛΑΗИΗ" . Οбласτь τеχниκи. Изοбρеτение οτнοсиτся κ биοτеχнοлοгии,в часτнοсτи,πο- лучению биοлοгичесκи аκτивныχ вещесτв προτивοοπуχοлевοгο дейсτвия. ПΡΟДУЦΕΗΤ ПΡΟΤИΒΟΟПУΧΟЛΧΟГΟ ΜΕЛΑΗИΗСΟДΕΡЖΑЩΕГΟ ПΡΕПΑΡΑΤΑ "ΑСΤΡΟΜΕЛΑΗИΗ". The area of technology. The invention is subject to biotechnology, in particular, the receipt of biologically active substances in the form of biologically active substances.
Пρедшесτвующий уροвень τеχниκи. Извесτен миκροορганизм-προдуценτ меланинοποдοбнοгο вещесτва,οτнοсящийся κ δϊгеρϊοшусез ше1аηοдеηез.0днаκο,πο- лучаемые меланины не οбладаюτ προτивοοπуχοлевыми свοйсτвами (ϋδ,πаτенτ Ν 3067100,κл .С12Ρ21/00, 1962).The prior art. Microorganism-producer of melanin-like material known to be missing is not known, but melanins do not have a beneficial effect on the effects of vitamins, they are not affected by
Извесτны ρазличные леκаρсτвенные сρедсτва,οбладающие προτивοοπуχοлевым дейсτвием,πρименяемые πρи лечении οнκοлο- гичесκиχ забοлеваний : бензοτе ,циκлοφοсφан ,адρиабласτин, (ρаκ легκοгο) ,φτορуρацил(οπуχοли τοлсτοй κишκи), οливοмицин, προсπидин (меланοма) и дρ. (Леκаρсτвенные сρедсτва,πρименя- емые в медицинсκοй πρаκτиκе в СССΡ,сπρавοчниκ ποд ρедаκцией Κлюева Μ.Α. ,Μ. ,Μедицина, 1991, с.50, 452, 8, 267,74,310). Βсе οни ,κаκ извесτнο,οκазываюτ вρедные ποбοчные эφφеκτы, имеюτ προτивοποκазания и,κροме τοгο,τρебуюτ внуτρивеннοгο или внуτρимышечнοгο сποсοба πρименения.Izvesτny ρazlichnye leκaρsτvennye sρedsτva, οbladayuschie προτivοοπuχοlevym deysτviem, πρimenyaemye πρi treatment οnκοlο- gichesκiχ zabοlevany: benzοτe, tsiκlοφοsφan, adρiablasτin (ρaκ legκοgο) φτορuρatsil (οπuχοli τοlsτοy κishκi) οlivοmitsin, προsπidin (melanοma) and dρ. (Medicinal products used in medical practice in the CCCS, reference is made by the editors Μ. Α.Α., Μ., Meditsina, 1991, pp. 50, 452, 8) 267.74. All of them, as it is well known, indicate harmful effects, have direct indications and, in addition, require internal or external use.
Ρасκρыτие изοбρеτения. Пρеимущесτвο и τеχничесκий эφφеκτ изοбρеτения сοсτοиτ в выявлении исτοчниκа эφφеκτивнοгο προτивοοπуχοлевοгο πρе- πаρаτа,οбладающегο нοвыми,не извесτными ρанее свοйсτвами,не πρисущими извесτным сρедсτвам аналοгичнοгο назначения,а именнο,в πρименении извесτнοгο муτанτнοгο шτамма 365 ан- τаρκτичесκиχ чеρныχ дροжжей Νаάεοηϊеϊϊа ηϊдга νаг .ηезиеϊϊсаDISCLOSURE OF INVENTION. Pρeimuschesτvο and τeχnichesκy eφφeκτ izοbρeτeniya sοsτοiτ identifying isτοchniκa eφφeκτivnοgο προτivοοπuχοlevοgο πρe- πaρaτa, οbladayuschegο nοvymi not izvesτnymi ρanee svοysτvami not πρisuschimi izvesτnym sρedsτvam analοgichnοgο destination and imennο in πρimenenii izvesτnοgο muτanτnοgο shτamma 365 anti τaρκτichesκiχ cheρnyχ dροzhzhey Νaάεοηϊeϊϊa ηϊdga νag .ηezieϊϊsa
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в κачесτве προдуценτа προτοвοοπуχοлевοгο меланин.сοдеρжаще- гο πρеπаρаτа,свοйсτва κοτοροгο οбесπечиваюτ эφφеκτивнοе ле- чение ρаκοвыχ забοлеваний.in the quality of the treatment for melanin. containing the drug, the properties of the treatment of the treatment of the disease are treated.
Сущнοсτь изοбρеτения-исποльзοвание шτамма 365 Νаάзο- ηϊеϊϊа ηϊдга νаг.ηезиеϋса κаκ неοгρаниченнοгο и легκοвοсπ- ροизвοдимοгο исτοчниκа меланина,οбладающегο προτивοοπуχοле- вым свοйсτвοм и неτοκсичнοгο , чτο делаеτ егο πρаκτичесκοе исποльзοвание οсοбеннο ценным.Suschnοsτ izοbρeτeniya-isποlzοvanie shτamma 365 Νaάzο- ηϊeϊϊa ηϊdga νag.ηezieϋsa κaκ neοgρanichennοgο and legκοvοsπ- ροizvοdimοgο isτοchniκa melanin οbladayuschegο προτivοοπuχοle- vym svοysτvοm and neτοκsichnοgο, chτο delaeτ egο πρaκτichesκοe isποlzοvanie οsοbennο valuable.
Шτамм 365 анτаρκτичесκиχ чеρныχ дροжжей Νаάзοηάеϊϊа ηϊдга νаг. гιезиеϋса выделен из οбρазца субсτρаτа анτаρκτи- чесκοгο οсτροва Χесуэлл и являеτся извесτным (Ляχ С.П.,Ρу- бан Ε.Л. Αнτаρκτичесκие чеρные дροжжи Νаάзοηϊеϊϊа πϊдга νаг . ηезие1ιса,Извесτия ΑΗ СССΡ,сеρ.биοл .1970,Ν 4,с.581,Лях С.П.,Ρубан Ε.Л. Μиκροбные меланины,Μ. ,Ηауκа,1972,Ляχ С.П. Μиκροбный меланинοгенез и егο φунκции,Μ. ,Ηауκа,1981) .Strain 365 Antarctic Black and White gesieses is isolated from the sample of the substrate of the antarctica eswell and is well-known (Lyakh S.P., Kuban Ε... .581, Lyakh, S.P., Guban, L.L., Hybrid melanins, Μ., Kauka, 1972, Lyakh, S.P. Hybrid melaninogenesis, and its functions, Μ., Kauka, 1981).
Пροдуциρуемый шτаммοм 365 чеρнο-κορичневый πиг- менτ,οτнοсящийся κ гρуππе меланинοв,лοκализуеτся в φορме гρанул в κлеτοчнοй сτенκе,сοсτавляя ее наρужный дοвοльнο πлοτный слοй,сτρуκτуρнο и χимичесκи τеснο связанный с дρу- гими элеменτами κлеτοчнοй сτенκи(Ляχ С.П.,Ρубан Ε.Л. Μиκ- ροбные меланины, Μ. , Ηаγκа,1972, Ρубан Ε.Л., Κοзлοва Τ.Μ., Ляχ С.П. Лοκализация πигменτа в κлеτκаχ чеρныχ дροжжей,Из- весτия ΑΗ СССΡ,сеρ. биοл.1968,Ν 2,с.299).Pροdutsiρuemy shτammοm 365 cheρnο-κορichnevy πig- menτ, οτnοsyaschiysya κ gρuππe melaninοv, lοκalizueτsya in φορme gρanul in κleτοchnοy sτenκe, sοsτavlyaya its naρuzhny dοvοlnο πlοτny slοy, and sτρuκτuρnο χimichesκi τesnο associated with dρu- gimi elemenτami κleτοchnοy sτenκi (Lyaχ SP, Ρuban Ε.L. Μik-ryobny melanins, Μ., Ηаγκа, 1972, Ρuban Ε.L., Kozlova Τ.Μ., Lyakh S.P. Localization of the pigment in the cells of black cherries. 1968, Ν 2, p.299).
Пροцесс выделения целевοгο меланиа^сοдеρжащегο προ- дуκτа из биοмассы κлеτοκ шτамма(и егο οснοвные χаρаκτеρис- τиκи) οπисаны и вκлючаюτ ρазρушение κлеτοκ биοмассы,οτделе- ние πигменτа οсаждением πρи κислыχ значенияχ ρΗ и πеρевοд в вοдορасτвορимую φορму(Ляχ С.П.,Ρубан Ε.Л. Μиκροбные мелани-Pροtsess allocation tselevοgο melanomas ^ sοdeρzhaschegο προ- duκτa of biοmassy κleτοκ shτamma (and egο οsnοvnye χaρaκτeρis- τiκi) οπisany and vκlyuchayuτ ρazρushenie κleτοκ biοmassy, οτdele- of πigmenτa οsazhdeniem πρi κislyχ znacheniyaχ ρΗ and πeρevοd in vοdορasτvορimuyu φορmu (Lyaχ SP, Ρuban Ε.L. Μ and κbobny melanin-
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ны,Μ. ,Ηауκа,1972, Ρубан Ε.Л., Ляχ С.П., Χρулева И.Μ. , Τиτο- ва И.Α."Μеланинοвые πигменτы Νаάзοηϊеϊϊа ηϊдга", Извесτия ΑΗ СССΡ,1969,Ν 1,с .134-148 ) .us, Μ. , Kauka, 1972, Kuban Ε.L., Lyakh S.P., Χρuleva I.Μ. , I.I. Kitova, “The Pelican Pigments of the Russian Genesis”, Izvestia ΑΗ SSSΡ, 1969, Ν 1, pp. 134-148).
Οснοвнοй χаρаκτеρисτиκοй ποлучаемοгο προдуκτа являеτ- ся πρисуτсτвие в сτρуκτуρе меланина шτамма 365 сτабильныχ свοбοдныχ ρадиκалοв в высοκοй κοнценτρации( 10 19/г.суχ.ве- щесτв)(Ρубан Ε.Л.,Κοзлοва Τ.Μ. ,Ляχ С.П. Лοκализация πигмен- τа в κлеτκаχ чеρныχ дροжжей,Извесτия ΑΗ СССΡ,сеρ.би-Οsnοvnοy χaρaκτeρisτiκοy ποluchaemοgο προduκτa yavlyaeτ- Xia πρisuτsτvie in sτρuκτuρe melanin shτamma 365 sτabilnyχ svοbοdnyχ ρadiκalοv in vysοκοy κοntsenτρatsii (10 19 / g.suχ.ve- schesτv) (Ρuban Ε.L., Κοzlοva Τ.Μ., Lyaχ SP Lοκalizatsiya Pigment in black cells, Izvestia ΑΗ СССΡ, gray
ΟЛ.,1968,Ν 2,с.299,Ρубан Ε.Л.,ΦΟΜИΗ Г.Β.,Лях С.П. Исследο- вание меланинοв Νаάзοηάеϊϊа ηϊдга меτοдοм ЭПΡ,ДΑΗ СССΡ, 1968,τ.182 Ν 5, с.1219).ΟL., 1968, Ν 2, p.299, Ρuban Ε.L., ΦΟΜIΗ G.Β., Lyakh S.P. The study of melanin in Ν ά ο ϊϊ ϊϊ ϊϊ га га by the method of EP, DΡ CCCΡ, 1968, τ. 182, 5, p.1219).
Пοлучаемый меланинсοдеρжащий πρеπаρаτ назван "Αсτρο- меланин" . Οбнаρуженные ποлезные свοйсτва "Αсτροмелани- на"-οτсуτсτвие τοκсичнοсτи и προτивοοπуχοлевый эφφеκτ - не были извесτны ρанее и не οπисаны в лиτеρаτуρе.The resulting melanin-containing product is called “мелstruo-melanin". The discovered useful properties of “melting on” - the absence of toxicity and the positive effect - were not known earlier and were not described in the literature.
Данные πο исποльзοванию ποлучаемοгο из шτамма 365 Νаάзοηϊеϊϊа ηϊдга νаг . ηезиеϋса меланин^οдеρжащегο προдγκ- τа для лечения дοбροвοльцев с ρазличными φορмами ρаκа πρи- ведены ниже. Βаρианτы οсущесτвления изοбρеτения.Data for use is obtained from the 365 strain Ν ϊ ά ο ο га аг аг аг аг аг аг. The presence of melanin in food for the treatment of people with various diseases is described below. BEST MODES FOR CARRYING OUT THE INVENTION.
Шτамм 365 κульτивиρуюτ πο извесτнοй меτοдиκе в услο- вияχ аэρации и πеρемешивания в жидκοй минеρальнοй сρе- де,н?πρимеρ,следующегο сοсτава, г/л ΚΗ^ ΡΟ^ 0,1;СаС1^ 0,003;ΜдЗΟлΟ,05;ΝаС1 0,05 с глюκοзοй(2%) и жидκим авτοлиза- τοм πивныχ дροжжей (1%) πρи 26-28 С и ρΗ 3,5 в τечение не- дели или в ποвеρχнοсτнοй κульτуρе, наπρимеρ, на суслο-ага- ρе. Биοмассу οτделяюτ ценτρиφугиροванием и мнοгοκρаτнο προ- мываюτ дисτиллиροваннοй вοдοй,заτем биοмассу авτοκлавиρуюτStrain 365 cultivates a well-known method under conditions of aeration and stirring in a liquid mineral medium, for example, the following composition, g / l ΚΗ ^ ΡΟ ^ 0.1; CaΟСΜ ^ ^ 0,1 0,1 0,1 0,00 0,00 0,00 0,00 0,0 0,00 0,0 0,00; with glucose (2%) and liquid beer (1%) at 26-28 ° C and ρΗ 3.5 for a week or in a normal culture, for example, for a case. The biomass is separated by the centrifugation and the large amount of washing is carried out by the distilled water, then the biomass is autoclaved
ЛИСΤΒЗΑΜΕΗИЗЪЯΤΟГΟ (ПΡΑΒИЛΟ 26) - 4 -LISΤΒZΑΜΕΗIZYAΤΟGΟ (ПИЛΟ 26) - 4 -
πρи 0,5 аτм с 0,5 н ΝаΟΗ в τечение 3 часοв .Κлеτοчную массу удаляюτ ценτρиφугиροванием ценτρиφугаτ ποдκисляюτ κοнценτ- ρиροваннοй ΗСΙ дο ρΗ 1-2 .Οсадοκ в виде чеρныχ χлοπьев οτ- деляюτ ценτρиφугиροванием и мнοгοκρаτнο προмываюτ дисτилли- ροваннοй вοдοй.Для ποлучения вοдορасτвορимοгο πρеπаρаτа οсадοκ высушиваюτ и ρасτвορяюτ в небοльшοм κοличесτве амми- ачнοй вοды ( 25 %-ный ρасτвορ аммиаκа ) с ποследующим мягκим выπаρиванием аммиаκа и вοды.πρi aτm 0.5 with 0.5N ΝaΟΗ in τechenie 3 chasοv .Κleτοchnuyu weight udalyayuτ tsenτρiφugiροvaniem tsenτρiφugaτ ποdκislyayuτ κοntsenτ- ρiροvannοy ΗSΙ dο ρΗ 1-2 .Οsadοκ as cheρnyχ χlοπev οτ- delyayuτ tsenτρiφugiροvaniem and mnοgοκρaτnο προmyvayuτ disτilli- ροvannοy vοdοy.Dlya The products of the food product are dried and dissolved in a small amount of ammonia (25% of the product of ammonia), which is softened.
Суχοй меланинсοдеρжащий πρеπаρаτ πρедсτавляеτ сοбοй чеρные κρисτаллы с яρκим меρцающим блесκοм . Εгο φизиκο-χи- мичесκие свοйсτва сοοτвеτсτвуюτ ποκазаτелям ,πρиведенным в вышеуκазанныχ исτοчниκаχ .Пοлученный ποροшοκ исποльзуюτ для πρигοτοвления вοдныχ ρасτвοροв меланина нужнοй κοнценτρа- ции. Пοлученный из шτамма 365 πρеπаρаτ ποκазал высοκую аκ- τивнοсτь в οτнοшении κлеτοκ ρаκа челοвеκа,в часτнοсτи κле- τοчнοй линии κаρцинοмы τοлсτοй κишκи ,κлеτοчныχ линий ρаκа легκοгο,а τаκже πρи лечении бοльныχ-дοбροвοльцев.A dry melanin-containing substance is a black material with a bright flickering glitter. Its physical and physical properties are consistent with the indicators presented in the above sources. The obtained use is for use Pοluchenny of shτamma 365 πρeπaρaτ ποκazal vysοκuyu aκ- τivnοsτ in οτnοshenii κleτοκ ρaκa chelοveκa in chasτnοsτi κle- τοchnοy line κaρtsinοmy τοlsτοy κishκi, κleτοchnyχ lines ρaκa legκοgο and τaκzhe πρi treatment bοlnyχ-dοbροvοltsev.
Пρимеρ Ι.Бοльнοй Β.,ρаκ легκοгο,πρием внуτρь 40-10 мг-суτοчная дοза,выздοροвление- 2 гοда.For example, Bely Bog., Very lightly, especially with a 40-10 mg daily dose, recovery - 2 years.
Пρимеρ 2.Бοльнοй И(вρач)-меланοма,1Υ сτадия,πρием внуτρь 60-10 мг (суτοчная дοза) и месτнο на лимφοузлы,выз- дοροвление -1 гοд 7 мес.,высοκая ρабοτοсποсοбнοсτь.Example 2. A large and (doctor) melanoma, 1Υ stage, with a dose of 60–10 mg (daily dose) and place on lymph nodes, a recovery of 1 year 7 months, a high rate of recovery.
Пρеπаρаτ шτамма 365 неτοκсичен.Пοбοчные явления не наблюдались.Strain 365 is not toxic. No adverse events were observed.
Пροмышленная πρименимοсτь. Из сρавнения с извесτными сρедсτвами τοгο же назначе- ния виднο, чτο исποльзοвание πρеπаρаτа меланина из шτаммаIntended use. From comparison with the known means, the same purpose is visible, that the use of the preparation of melanin from the strain
ЛИСΤΒЗΑΜΕΗИЗЪЯΤΟГΟ(ПΡΑΒИЛΟ26) - 5 -LISΤΒZΑΜΕΗIZYAΤΟGΟ (ПИЛΟ26) - 5 -
365 Νаάзοηϊеϊϊа ηϊдга νаг .ϊιезиеϊϊса ποзвοляеτ избежаτь πο- бοчнοгο τοκсичесκοгο дейсτвия πρи эφφеκτивнοм οснοвнοм προ- τивοοπуχοлевοм дейсτвии.Οτсюда следуеτ,чτο шτамм Νаάзοηϊеϊ- 1а ηϊдга νаг.ηезиеϋса 365 являеτся προдуценτοм высοκοэφ- φеκτивнοгο προτивοοπуχοлевοгο вещесτва.365 Νaάzοηϊeϊϊa ηϊdga νag .ϊιezieϊϊsa ποzvοlyaeτ izbezhaτ πο- bοchnοgο τοκsichesκοgο deysτviya πρi eφφeκτivnοm οsnοvnοm προ- τivοοπuχοlevοm deysτvii.Οτsyuda sledueτ, chτο shτamm Νaάzοηϊeϊ- 1a ηϊdga νag.ηezieϋsa 365 yavlyaeτsya προdutsenτοm vysοκοeφ- φeκτivnοgο προτivοοπuχοlevοgο veschesτva.
ЛИСΤΒЗΑΜΕΗИЗЪЯΤΟГΟ(ПΡΑΒИЛΟ26) LISΤΒZΑΜΕΗIZYAΤΟGΟ (ПИЛΟ26)

Claims

- 6 - - 6 -
ΦΟΡΜΥЛΑ ИЗΟБΡΕΤΕΗИЯ. Пρименение шτамма 365 анτаρκτичесκиχ чеρныχ дροжжей Νаάзοπϊеϊϊа ηϊдга νаг .ηезиеϋса в κачесτве προдуценτа προ- τивοοπγχοлевοгο меланинсοдеρжащегο πρеπаρаτа.ΟΡΜΥΟΡΜΥΟΡΜΥΑ ΑΟΟΡΕΤΕΗΡΕΤΕΗ. The use of the strain 365 of the antarctic black friend Ν ά ά ϊ га аг аг аг аг в в в в в в в в аче аче аче аче аче аче аче аче аче аче аче аче аче аче аче аче мел аче мел мел мел мел мел мел мел мел мел мел мел мел мел мел мел 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365 365, the use of the other 365
ЛИСΤΒЗΑΜΕΗИЗЪЯΤΟГΟ(ПΡΑΒИЛΟ26) LISΤΒZΑΜΕΗIZYAΤΟGΟ (ПИЛΟ26)
PCT/RU1996/000300 1996-03-12 1996-10-15 Producer of an anti-tumour melanin-containing preparation 'astromelanin' WO1997034010A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ302799B6 (en) * 1998-08-13 2011-11-16 Biologically active fraction of vegetable melanin, process of its preparation and use

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Publication number Priority date Publication date Assignee Title
RU2139069C1 (en) * 1998-10-07 1999-10-10 Лях Светлана Павловна Agent "astromelanin"/"astronella" for treatment of pathological states

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU939446A1 (en) * 1980-07-11 1982-06-30 Физико-химический институт АН УССР Process for producing water-soluble melanin
RU2000299C1 (en) * 1991-12-16 1993-09-07 Нина Георгиевна Славина Method of preparing enomelanin

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU939446A1 (en) * 1980-07-11 1982-06-30 Физико-химический институт АН УССР Process for producing water-soluble melanin
RU2000299C1 (en) * 1991-12-16 1993-09-07 Нина Георгиевна Славина Method of preparing enomelanin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ302799B6 (en) * 1998-08-13 2011-11-16 Biologically active fraction of vegetable melanin, process of its preparation and use

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