WO1997008551A1 - Procedes et anticorps permettant la detection de creatine kinase - Google Patents
Procedes et anticorps permettant la detection de creatine kinase Download PDFInfo
- Publication number
- WO1997008551A1 WO1997008551A1 PCT/US1996/013755 US9613755W WO9708551A1 WO 1997008551 A1 WO1997008551 A1 WO 1997008551A1 US 9613755 W US9613755 W US 9613755W WO 9708551 A1 WO9708551 A1 WO 9708551A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- isoenzyme
- assay
- binding substance
- antibody
- isoenzymes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/70—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving creatine or creatinine
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9123—Phosphotransferases in general with a nitrogenous group as acceptor (2.7.3), e.g. histidine kinases
Definitions
- Patent 4,624,961 describes two-site immunoradiometric assays for detecting CK-MB isoenzyme where cross-reactivity is reduced by performing the assay in the presence of pooled human sera.
- the detection of specific isoforms of the MM, BB and MB CK isoenzymes is described in the following U. S. Patent Nos. 4,810,639; 4,900,662; 5,202,234; 5,382,515;
- the antibody will have a substantially equal binding affinity for each of the CK-MM, CK-BB, and CK-MB isoforms, and mitochondrial CK.
- the use of such assays permits detection of total CK isoenzyme using only a single antibody which is cross-reactive with each of the three CK isoenzymes.
- mixtures of antibodies specific for the M subunit and B subunit, respectively can be used for binding total CK isoenzyme in the methods of the present invention.
- the total CK isoenzyme (including all three isoenzymes) and the CK-MB isoenzyme will be measured by reacting the sample with at least one binding substance which binds to the target enzyme, usually an antibody, an antibody fragment, or equivalent molecule, as described in more detail below.
- at least one binding substance which binds to the target enzyme usually an antibody, an antibody fragment, or equivalent molecule, as described in more detail below.
- binding substance and “specific binding substance” will refer to intact antibody molecules (immunoglobulins) as well as to portions of the antibody molecule which retain the ability to bind to epitopes on the target enzyme.
- Such antibody portions include the Fab, Fab', and F(ab') 2 fragments of immunoglobulins.
- numerous techniques have been prepared for recombinantly producing molecules having the binding specificities of antibodies.
- an antibody molecule is prepared in a conventional manner, as described in more detail below, and the gene encoding the antibody molecule isolated. After sequencing the antibody gene, recombinant nucleic acids can be isolated or synthesized and used to prepare binding proteins by a variety of now conventional methods.
- the epitope should be sufficiently sterically removed from the epitope which is bound to the solid phase to permit detection with the labeling reagent.
- a mixture of antibodies specific for the M and B subunits may be employed, where the antibodies are attached to the same label.
- Suitable labels will be of a type which are separately detectable within each of the reaction zones. After the initial steps of binding the CK isoenzyme and labeling the bound CK isoenzyme, it is necessary to separately detect the amount of isoenzyme bound within each reaction zone.
- radio and enzyme labels that do not produce a localized signal will generally not be suitable for use in the present invention. Localized labels, such as fluorescent labels, luminescent labels, and the like will find particular use.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU67641/96A AU6764196A (en) | 1995-08-31 | 1996-08-26 | Methods and antibodies for detecting creatine kinase |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52204895A | 1995-08-31 | 1995-08-31 | |
US08/522,048 | 1995-08-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997008551A1 true WO1997008551A1 (fr) | 1997-03-06 |
Family
ID=24079248
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1996/013755 WO1997008551A1 (fr) | 1995-08-31 | 1996-08-26 | Procedes et anticorps permettant la detection de creatine kinase |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU6764196A (fr) |
WO (1) | WO1997008551A1 (fr) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6136610A (en) * | 1998-11-23 | 2000-10-24 | Praxsys Biosystems, Inc. | Method and apparatus for performing a lateral flow assay |
EP1072889A1 (fr) * | 1999-01-19 | 2001-01-31 | International Reagents Corporation | Procede d'analyse de l'activite de l'isozyme de la creatine kinase et reactif d'analyse |
WO2002004950A2 (fr) * | 2000-07-12 | 2002-01-17 | Werner Naser | Evaluation directe de rapports analyte/molecule de reference |
WO2002008722A2 (fr) * | 2000-07-12 | 2002-01-31 | Werner Naser | Evaluation directe de concentrations relatives de variants d'un epitope sur une molecule dimere |
US6528323B1 (en) | 1999-06-14 | 2003-03-04 | Praxsys Biosystems, Inc. | Bidirectional lateral flow test strip and method |
EP1359162A2 (fr) * | 2002-04-30 | 2003-11-05 | International Reagents Corporation | Anticorps anti-créatine kinase mitochondrienne |
US7605004B2 (en) | 2001-07-18 | 2009-10-20 | Relia Diagnostic Systems Llc | Test strip for a lateral flow assay for a sample containing whole cells |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116731185B (zh) * | 2023-06-27 | 2023-11-10 | 安徽千诚生物技术有限公司 | 一种肌酸激酶同工酶抗体及线粒体ck检测试剂盒 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4353982A (en) * | 1980-04-10 | 1982-10-12 | Hoffmann-La Roche Inc. | Immunochemical assay for creatine kinase-MB isoenzyme |
US5017687A (en) * | 1988-03-10 | 1991-05-21 | Virovahl, S.A. | Peptides for the detection of HTLV-1 infection |
US5210039A (en) * | 1989-02-09 | 1993-05-11 | Eastman Kodak Company | Wash composition, test kit and their use to determine a herpes simplex viral antigen |
US5290678A (en) * | 1990-10-12 | 1994-03-01 | Spectral Diagnostics Inc. | Diagnostic kit for diagnosing and distinguishing chest pain in early onset thereof |
US5298406A (en) * | 1992-09-14 | 1994-03-29 | E. I. Du Pont De Nemours And Company | Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes |
-
1996
- 1996-08-26 AU AU67641/96A patent/AU6764196A/en not_active Abandoned
- 1996-08-26 WO PCT/US1996/013755 patent/WO1997008551A1/fr active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4353982A (en) * | 1980-04-10 | 1982-10-12 | Hoffmann-La Roche Inc. | Immunochemical assay for creatine kinase-MB isoenzyme |
US5017687A (en) * | 1988-03-10 | 1991-05-21 | Virovahl, S.A. | Peptides for the detection of HTLV-1 infection |
US5210039A (en) * | 1989-02-09 | 1993-05-11 | Eastman Kodak Company | Wash composition, test kit and their use to determine a herpes simplex viral antigen |
US5290678A (en) * | 1990-10-12 | 1994-03-01 | Spectral Diagnostics Inc. | Diagnostic kit for diagnosing and distinguishing chest pain in early onset thereof |
US5298406A (en) * | 1992-09-14 | 1994-03-29 | E. I. Du Pont De Nemours And Company | Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes |
Non-Patent Citations (3)
Title |
---|
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 14 November 1986, Vol. 140, No. 3, PERRYMAN et al., "Isolation and Sequence Analysis of a Full-Length cDNA for Human M Creatine Kinase", pages 981-989. * |
MOLECULAR AND CELLULAR BIOCHEMISTRY, April/May 1994, Vols. 133/134, MUHLEBACH et al., "IV-2 Sequence Homology and Structure Predictions of the Creatine Kinase Isoenzymes", pages 245-262. * |
N. TIETZ, "Textbook of Clinical Chemistry", published 1986, by W.B. SAUNDERS COMPANY (PHILADELPHIA), see pages 686-689, 698-699, 1820. * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6136610A (en) * | 1998-11-23 | 2000-10-24 | Praxsys Biosystems, Inc. | Method and apparatus for performing a lateral flow assay |
EP1072889A1 (fr) * | 1999-01-19 | 2001-01-31 | International Reagents Corporation | Procede d'analyse de l'activite de l'isozyme de la creatine kinase et reactif d'analyse |
EP1072889A4 (fr) * | 1999-01-19 | 2005-10-19 | Int Reagents Corp | Procede d'analyse de l'activite de l'isozyme de la creatine kinase et reactif d'analyse |
US6586194B1 (en) * | 1999-01-19 | 2003-07-01 | International Reagents Corporation | Method for assaying creatine kinase isozyme activity and assay reagent |
US6528323B1 (en) | 1999-06-14 | 2003-03-04 | Praxsys Biosystems, Inc. | Bidirectional lateral flow test strip and method |
US7229839B2 (en) | 1999-06-14 | 2007-06-12 | Relia Diagnostic Systems, Llc | Bidirectional lateral flow test strip and method |
WO2002008722A2 (fr) * | 2000-07-12 | 2002-01-31 | Werner Naser | Evaluation directe de concentrations relatives de variants d'un epitope sur une molecule dimere |
WO2002008722A3 (fr) * | 2000-07-12 | 2002-05-16 | Werner Naser | Evaluation directe de concentrations relatives de variants d'un epitope sur une molecule dimere |
WO2002004950A3 (fr) * | 2000-07-12 | 2002-04-18 | Werner Naser | Evaluation directe de rapports analyte/molecule de reference |
WO2002004950A2 (fr) * | 2000-07-12 | 2002-01-17 | Werner Naser | Evaluation directe de rapports analyte/molecule de reference |
US7605004B2 (en) | 2001-07-18 | 2009-10-20 | Relia Diagnostic Systems Llc | Test strip for a lateral flow assay for a sample containing whole cells |
EP1359162A2 (fr) * | 2002-04-30 | 2003-11-05 | International Reagents Corporation | Anticorps anti-créatine kinase mitochondrienne |
EP1359162A3 (fr) * | 2002-04-30 | 2004-01-14 | International Reagents Corporation | Anticorps anti-créatine kinase mitochondrienne |
Also Published As
Publication number | Publication date |
---|---|
AU6764196A (en) | 1997-03-19 |
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