WO1996039195A3 - Oligonucleotide chimiquement modifie pour mutagenese dirigee sur site - Google Patents

Oligonucleotide chimiquement modifie pour mutagenese dirigee sur site Download PDF

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Publication number
WO1996039195A3
WO1996039195A3 PCT/US1996/008883 US9608883W WO9639195A3 WO 1996039195 A3 WO1996039195 A3 WO 1996039195A3 US 9608883 W US9608883 W US 9608883W WO 9639195 A3 WO9639195 A3 WO 9639195A3
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WO
WIPO (PCT)
Prior art keywords
chemically modified
site
directed mutagenesis
modified oligonucleotide
molecule
Prior art date
Application number
PCT/US1996/008883
Other languages
English (en)
Other versions
WO1996039195A2 (fr
Inventor
Peter M Glazer
Pamela A Havre
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Yale filed Critical Univ Yale
Priority to AU60432/96A priority Critical patent/AU6043296A/en
Publication of WO1996039195A2 publication Critical patent/WO1996039195A2/fr
Publication of WO1996039195A3 publication Critical patent/WO1996039195A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3511—Conjugate intercalating or cleaving agent

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne un oligonucléotide mutagène formant un triplex et ses procédés d'utilisation. L'oligonucléotide est chimiquement modifié afin de contenir un mutagène incorporé et forme une molécule d'acide nucléique à trois brins avec un segment spécifique d'ADN d'une molécule cible d'ADN. Lorsque le triplex est formé, le mutagène est rapproché de la molécule cible et provoque une mutation sur un site spécifique de celle-ci. La mutation active, désactive ou modifie l'activité et la fonction de la molécule cible.
PCT/US1996/008883 1995-06-06 1996-06-04 Oligonucleotide chimiquement modifie pour mutagenese dirigee sur site WO1996039195A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU60432/96A AU6043296A (en) 1995-06-06 1996-06-04 Chemically modified oligonucleotide for site-directed mutage nesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46351995A 1995-06-06 1995-06-06
US463,519 1995-06-06

Publications (2)

Publication Number Publication Date
WO1996039195A2 WO1996039195A2 (fr) 1996-12-12
WO1996039195A3 true WO1996039195A3 (fr) 1997-01-30

Family

ID=23840376

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/008883 WO1996039195A2 (fr) 1995-06-06 1996-06-04 Oligonucleotide chimiquement modifie pour mutagenese dirigee sur site

Country Status (2)

Country Link
AU (1) AU6043296A (fr)
WO (1) WO1996039195A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
US11918695B2 (en) 2014-05-09 2024-03-05 Yale University Topical formulation of hyperbranched polymer-coated particles
EP3139909A4 (fr) 2014-05-09 2018-01-03 Yale University Formulation topique de particules enrobées de polyglycérol hyperramifié
WO2017143042A2 (fr) 2016-02-16 2017-08-24 Yale University Compositions permettant d'améliorer l'édition ciblée de gènes et leurs procédés d'utilisation
AU2017221424A1 (en) 2016-02-16 2018-09-20 Yale University Compositions and methods for treatment of cystic fibrosis
WO2018187493A1 (fr) 2017-04-04 2018-10-11 Yale University Compositions et procédés d'administration in utero
US20210189431A1 (en) 2018-08-10 2021-06-24 Yale University Compositions and methods for embryonic gene editing in vitro
KR20210054547A (ko) 2018-08-31 2021-05-13 예일 유니버시티 삼중체 및 뉴클레아제 기반 유전자 편집을 향상시키기 위한 조성물 및 방법
WO2020112195A1 (fr) 2018-11-30 2020-06-04 Yale University Compositions, technologies et procédés d'utilisation de plérixafor pour améliorer l'édition de gènes
US20220243211A1 (en) 2019-06-21 2022-08-04 Yale University Peptide nucleic acid compositions with modified hoogsteen binding segments and methods of use thereof
US20220372474A1 (en) 2019-06-21 2022-11-24 Yale University Hydroxymethyl-modified gamma-pna compositions and methods of use thereof
US20220339294A1 (en) 2019-09-09 2022-10-27 Yale University Nanoparticles for selective tissue or cellular uptake

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (fr) * 1986-07-08 1988-01-20 Abbott Laboratories Méthode de mutagénèse par réparation dirigée à l'aide d'oligonucléotides d'une rupture de brin
US5354670A (en) * 1991-12-24 1994-10-11 The President And Fellows Of Harvard College Site-directed mutagenesis of DNA
WO1995001364A1 (fr) * 1993-06-25 1995-01-12 Yale University Oligonucleotide modifie chimiquement en vue d'une mutagenese dirigee vers le site

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (fr) * 1986-07-08 1988-01-20 Abbott Laboratories Méthode de mutagénèse par réparation dirigée à l'aide d'oligonucléotides d'une rupture de brin
US5354670A (en) * 1991-12-24 1994-10-11 The President And Fellows Of Harvard College Site-directed mutagenesis of DNA
WO1995001364A1 (fr) * 1993-06-25 1995-01-12 Yale University Oligonucleotide modifie chimiquement en vue d'une mutagenese dirigee vers le site

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GASPARRO F P ET AL: "Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: Characterization of psoralen monoadduct and crosslink formation.", NUCLEIC ACIDS RESEARCH 22 (14). 1994. 2845-2852. ISSN: 0305-1048, XP002018568 *
HAVRE P A ET AL: "Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.", JOURNAL OF VIROLOGY 67 (12). 1993. 7324-7331. ISSN: 0022-538X, XP002018570 *
HAVRE PA ET AL: "Targeted mutagenesis of DNA using triple helix-forming oligonucleotides linked to psoralen.", PROC NATL ACAD SCI U S A, AUG 15 1993, 90 (16) P7879-83, UNITED STATES, XP002018569 *
RAHA M ET AL: "Mutagenesis by third-strand-directed psoralen adducts in repair-deficient human cells: high frequency and altered spectrum in a xeroderma pigmentosum variant.", PROC NATL ACAD SCI U S A, APR 2 1996, 93 (7) P2941-6, UNITED STATES, XP002018566 *
WANG G ET AL: "Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation.", MOLECULAR AND CELLULAR BIOLOGY 15 (3). 1995. 1759-1768. ISSN: 0270-7306, XP002018567 *

Also Published As

Publication number Publication date
WO1996039195A2 (fr) 1996-12-12
AU6043296A (en) 1996-12-24

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