WO1996021042B1 - Amorces destinees a l'amplification pcr de sequences metastatiques - Google Patents
Amorces destinees a l'amplification pcr de sequences metastatiquesInfo
- Publication number
- WO1996021042B1 WO1996021042B1 PCT/US1996/000461 US9600461W WO9621042B1 WO 1996021042 B1 WO1996021042 B1 WO 1996021042B1 US 9600461 W US9600461 W US 9600461W WO 9621042 B1 WO9621042 B1 WO 9621042B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- seq
- metastatic
- sequence
- primers
- prostate
- Prior art date
Links
- 206010061289 Metastatic neoplasm Diseases 0.000 title claims abstract 21
- 230000001394 metastastic Effects 0.000 title claims abstract 18
- 230000003321 amplification Effects 0.000 title claims abstract 8
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract 8
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract 14
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 11
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims abstract 10
- 108020004707 nucleic acids Proteins 0.000 claims abstract 8
- 210000001519 tissues Anatomy 0.000 claims abstract 7
- 229920002676 Complementary DNA Polymers 0.000 claims abstract 6
- 206010060862 Prostate cancer Diseases 0.000 claims abstract 6
- 239000012472 biological sample Substances 0.000 claims abstract 6
- 239000002299 complementary DNA Substances 0.000 claims abstract 6
- 238000001514 detection method Methods 0.000 claims abstract 4
- 201000001514 prostate carcinoma Diseases 0.000 claims abstract 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims abstract 3
- 229920001850 Nucleic acid sequence Polymers 0.000 claims abstract 3
- 239000012188 paraffin wax Substances 0.000 claims abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract 2
- 239000000523 sample Substances 0.000 claims 13
- 239000002773 nucleotide Substances 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 102000007066 Prostate-Specific Antigen Human genes 0.000 claims 9
- 210000002307 Prostate Anatomy 0.000 claims 7
- 229920000665 Exon Polymers 0.000 claims 3
- 101710027066 ALB Proteins 0.000 claims 2
- 102100001249 ALB Human genes 0.000 claims 2
- 102000013563 Acid Phosphatase Human genes 0.000 claims 2
- 108010051457 Acid Phosphatase Proteins 0.000 claims 2
- 239000004382 Amylase Substances 0.000 claims 2
- 102000013142 Amylases Human genes 0.000 claims 2
- 108010065511 Amylases Proteins 0.000 claims 2
- 229920002459 Intron Polymers 0.000 claims 2
- 210000003563 Lymphoid Tissue Anatomy 0.000 claims 2
- 102100019730 TP53 Human genes 0.000 claims 2
- 101710026335 TP53 Proteins 0.000 claims 2
- 229940050528 albumin Drugs 0.000 claims 2
- 235000019418 amylase Nutrition 0.000 claims 2
- 102000015694 estrogen receptors Human genes 0.000 claims 2
- 108010038795 estrogen receptors Proteins 0.000 claims 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims 2
- 239000008098 formaldehyde solution Substances 0.000 claims 2
- 238000007710 freezing Methods 0.000 claims 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- 102000003998 progesterone receptors Human genes 0.000 claims 2
- 108090000468 progesterone receptors Proteins 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 210000000481 Breast Anatomy 0.000 claims 1
- 102100013472 CCK Human genes 0.000 claims 1
- 210000003679 Cervix Uteri Anatomy 0.000 claims 1
- 108010048926 Cholecystokinin Proteins 0.000 claims 1
- 229940107137 Cholecystokinin Drugs 0.000 claims 1
- 101700011961 DPOM Proteins 0.000 claims 1
- 238000009007 Diagnostic Kit Methods 0.000 claims 1
- 210000004696 Endometrium Anatomy 0.000 claims 1
- 102100009984 FAP Human genes 0.000 claims 1
- 101710008097 FAP Proteins 0.000 claims 1
- 101700053916 GLMN Proteins 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 102000003746 Insulin Receptor Human genes 0.000 claims 1
- 108010001127 Insulin Receptor Proteins 0.000 claims 1
- 102000023577 LH Receptors Human genes 0.000 claims 1
- 108010011942 LH Receptors Proteins 0.000 claims 1
- 210000004185 Liver Anatomy 0.000 claims 1
- 210000004072 Lung Anatomy 0.000 claims 1
- 102000009151 Luteinizing Hormone Human genes 0.000 claims 1
- 108010073521 Luteinizing Hormone Proteins 0.000 claims 1
- 229940040129 Luteinizing Hormone Drugs 0.000 claims 1
- 101710029649 MDV043 Proteins 0.000 claims 1
- 206010027476 Metastasis Diseases 0.000 claims 1
- 101700061424 POLB Proteins 0.000 claims 1
- 210000000496 Pancreas Anatomy 0.000 claims 1
- 101700054624 RF1 Proteins 0.000 claims 1
- 210000003932 Urinary Bladder Anatomy 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 claims 1
- PKVZBNCYEICAQP-UHFFFAOYSA-N hydron;N,2,2,3-tetramethylbicyclo[2.2.1]heptan-3-amine;chloride Chemical compound Cl.C1CC2C(C)(C)C(NC)(C)C1C2 PKVZBNCYEICAQP-UHFFFAOYSA-N 0.000 claims 1
- 230000000683 nonmetastatic Effects 0.000 claims 1
- 230000001817 pituitary Effects 0.000 claims 1
- 238000002203 pretreatment Methods 0.000 claims 1
- 238000007039 two-step reaction Methods 0.000 claims 1
- 102100011339 KLK3 Human genes 0.000 abstract 2
- 206010006187 Breast cancer Diseases 0.000 abstract 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 abstract 1
- 201000008275 breast carcinoma Diseases 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 201000005296 lung carcinoma Diseases 0.000 abstract 1
Abstract
L'invention se rapporte à des procédés de détection de maladies métastatiques telles que les cancers métastatiques de la prostate, du sein et du poumon, dans des échantillons biologiques frais ou fixés sur un support. Des acides nucléiques sont purifiés à partir d'échantillons fixés d'un fluide ou tissu d'un patient que l'on soupçonne de contenir des tissus métastatiques, et subissent une transcription inverse en ADNc. Des séquences spécifiques des métastases dans l'ADNc sont amplifiées par réaction d'amplification par polymérase. Des amorces destinées à l'amplification PCR comprennent des séquences provenant des régions exoniques d'un produit d'expression spécifique des métastases qui couvrent des régions d'ARN exprimé qui ne sont pas dégradées, même après le stockage de longue durée dans la paraffine. Les produits d'expression spécifiques des métastases comprennent, par exemple, des acides nucléiques qui contiennent des séquences du gène de l'antigène spécifique de la prostate pouvant être utilisées pour détecter les cancers métastatiques de la prostate. Les séquences nucléotidiques amplifiées obtenues comprendront des séquences spécifiques des métastases qui peuvent aisément être détectées. L'invention se rapporte également à des amorces d'acide nucléique qui peuvent être utilisées pour l'amplification PCR des séquences spécifiques des métastases telles que des séquences qui correspondent aux régions exoniques du gène PSA, et à des trousses comprenant ces amorces et d'autres réactifs convenant à la détection rapide des maladies métastatiques.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU48978/96A AU4897896A (en) | 1995-01-04 | 1996-01-04 | Primers for the pcr amplification of metastatic sequences |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36870695A | 1995-01-04 | 1995-01-04 | |
| US08/368,706 | 1995-01-04 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| WO1996021042A2 WO1996021042A2 (fr) | 1996-07-11 |
| WO1996021042A3 WO1996021042A3 (fr) | 1996-09-12 |
| WO1996021042B1 true WO1996021042B1 (fr) | 1996-10-17 |
| WO1996021042A9 WO1996021042A9 (fr) | 1996-11-28 |
Family
ID=23452406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/000461 WO1996021042A2 (fr) | 1995-01-04 | 1996-01-04 | Amorces destinees a l'amplification pcr de sequences metastatiques |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU4897896A (fr) |
| WO (1) | WO1996021042A2 (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103237A (en) * | 1993-07-22 | 2000-08-15 | Hybritech Incorporated | Stable variant hK2 polypeptide |
| WO1995030758A1 (fr) * | 1994-05-10 | 1995-11-16 | Mayo Foundation For Medical Education And Research | Polypeptide hk2 recombine |
| US5919638A (en) * | 1996-10-08 | 1999-07-06 | Abbott Laboratories | Reagents and methods useful for detecting prostate tumors |
| ATE375403T1 (de) * | 1996-11-06 | 2007-10-15 | Sequenom Inc | Dna-diagnostik mittels massenspektrometrie |
| EP0941368A2 (fr) * | 1996-11-14 | 1999-09-15 | Mayo Foundation For Medical Education And Research | Procede de detection du cancer de la prostate metastatique |
| US6479263B1 (en) * | 1996-11-14 | 2002-11-12 | Baylor College Of Medicine | Method for detection of micrometastatic prostate cancer |
| US6235486B1 (en) | 1997-06-20 | 2001-05-22 | Mayo Foundation For Medical Education & Research | Method for detection of breast cancer |
| US5972615A (en) | 1998-01-21 | 1999-10-26 | Urocor, Inc. | Biomarkers and targets for diagnosis, prognosis and management of prostate disease |
| WO1999037811A1 (fr) * | 1998-01-21 | 1999-07-29 | Urocor, Inc. | Marqueurs biochimiques et cibles pour le diagnostic, le pronostic et le traitement des affections de la prostate |
| ATE391176T1 (de) * | 1998-10-07 | 2008-04-15 | Roche Diagnostics Gmbh | Neues gen mit induzierter expression in menschlichen metastatischen tumorzellen |
| WO2000044940A2 (fr) * | 1999-01-28 | 2000-08-03 | Gen-Probe Incorporated | Sequences d'acide nucleique permettant de detecter des marqueurs genetiques pour le cancer dans un echantillon biologique |
| WO2002081656A2 (fr) * | 2001-04-05 | 2002-10-17 | The Henry M. Jackson Foundation | Potentiel diagnostique ameliore de cellules exprimant l'antigene specifique de la prostate |
| EP1572105B1 (fr) * | 2002-11-14 | 2011-08-31 | John Wayne Cancer Institute | Detection de micro metastases de melanomes et de cancer du sein dans des ganglions lymphatiques drainant des tumeurs incorporees dans de la paraffine par rt-pcr quantitative a l'aide de marqueurs multiples |
| CN113549689B (zh) * | 2021-09-23 | 2021-12-24 | 默禾医疗科技（上海）有限公司 | 一种检测pros1基因外显子的试剂盒及方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05506151A (ja) * | 1990-02-07 | 1993-09-16 | エフ．ホフマン ― ラ ロシュ アーゲー | Ｇｔｐ結合蛋白質をコードする遺伝子における点突然変異の検出 |
| AU662906B2 (en) * | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
| EP0667920B1 (fr) * | 1992-10-29 | 2003-01-15 | Thomas Jefferson University | Procedes de detection de micrometastase du cancer de la prostate |
| GB9309966D0 (en) * | 1993-05-14 | 1993-06-30 | Nordion Int Inc | Detection of prostrate-specific antigen in breast tumors |
-
1996
- 1996-01-04 WO PCT/US1996/000461 patent/WO1996021042A2/fr active Application Filing
- 1996-01-04 AU AU48978/96A patent/AU4897896A/en not_active Abandoned
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