WO1996007755A3 - Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria - Google Patents

Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria Download PDF

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Publication number
WO1996007755A3
WO1996007755A3 PCT/CZ1995/000020 CZ9500020W WO9607755A3 WO 1996007755 A3 WO1996007755 A3 WO 1996007755A3 CZ 9500020 W CZ9500020 W CZ 9500020W WO 9607755 A3 WO9607755 A3 WO 9607755A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorochrome
indicators
substrates
pathogenic bacteria
potentially pathogenic
Prior art date
Application number
PCT/CZ1995/000020
Other languages
French (fr)
Other versions
WO1996007755A2 (en
Inventor
Bozena Sedlinska
Jin Haeusler
Vlastimil Stetina
Original Assignee
Lachema As
Bozena Sedlinska
Jin Haeusler
Vlastimil Stetina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lachema As, Bozena Sedlinska, Jin Haeusler, Vlastimil Stetina filed Critical Lachema As
Publication of WO1996007755A2 publication Critical patent/WO1996007755A2/en
Publication of WO1996007755A3 publication Critical patent/WO1996007755A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria, based on that onto a moistened adsorption matrix saturated with a fluorochrome substrate there is transfered from a selective culture medium an incubated membrane filter with colonies of microorganisms which upon further cultivation for 1 to 4 hours at an optimal temperature for the microorganisms in question is exposed to U.V. rays of a wave length of 350 to 370 nm.
PCT/CZ1995/000020 1994-09-09 1995-09-11 Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria WO1996007755A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CZ942192A CZ282401B6 (en) 1994-09-09 1994-09-09 Method of making conformity tests by making use of fluorochrome substrata in indicators of faecal contamination or potentially pathogenic bacteria
CZPV2192-94 1994-09-09

Publications (2)

Publication Number Publication Date
WO1996007755A2 WO1996007755A2 (en) 1996-03-14
WO1996007755A3 true WO1996007755A3 (en) 1996-07-25

Family

ID=5464527

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CZ1995/000020 WO1996007755A2 (en) 1994-09-09 1995-09-11 Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria

Country Status (2)

Country Link
CZ (1) CZ282401B6 (en)
WO (1) WO1996007755A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019001357A2 (en) 2016-08-12 2019-04-30 Coloplast As ostomy appliance

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
WO1986001903A1 (en) * 1984-09-19 1986-03-27 Board Of Regents, The University Of Texas System Colony blot assay for enterotoxigenic bacteria
US4591554A (en) * 1979-10-31 1986-05-27 Ajinomoto Co., Inc. Rapid method for detecting microorganisms
GB2178847A (en) * 1985-08-07 1987-02-18 Philips Electronic Associated Testing for the presence of living organisms at the surface of an object
WO1990012888A1 (en) * 1989-04-27 1990-11-01 Biocontrol Systems, Incorporated Precipitate test for microorganisms

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591554A (en) * 1979-10-31 1986-05-27 Ajinomoto Co., Inc. Rapid method for detecting microorganisms
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
WO1986001903A1 (en) * 1984-09-19 1986-03-27 Board Of Regents, The University Of Texas System Colony blot assay for enterotoxigenic bacteria
GB2178847A (en) * 1985-08-07 1987-02-18 Philips Electronic Associated Testing for the presence of living organisms at the surface of an object
WO1990012888A1 (en) * 1989-04-27 1990-11-01 Biocontrol Systems, Incorporated Precipitate test for microorganisms

Also Published As

Publication number Publication date
WO1996007755A2 (en) 1996-03-14
CZ219294A3 (en) 1996-03-13
CZ282401B6 (en) 1997-07-16

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