WO1995033986A1 - Modified siphons for improved metering precision - Google Patents
Modified siphons for improved metering precision Download PDFInfo
- Publication number
- WO1995033986A1 WO1995033986A1 PCT/US1995/007145 US9507145W WO9533986A1 WO 1995033986 A1 WO1995033986 A1 WO 1995033986A1 US 9507145 W US9507145 W US 9507145W WO 9533986 A1 WO9533986 A1 WO 9533986A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- chamber
- rotor
- siphon
- inlet
- liquid
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
Definitions
- the present invention relates generally to devices and methods for analyzing biological fluids.
- it relates to the design and use of improved centrifugal rotors having siphons which allow delivery of a precise volume of liquid to a chamber in the rotor.
- Biological tests of blood plasma and other biological fluids frequently require that fluids be quickly divided into predetermined volumes for analysis in a variety of optical tests or assays. It is also frequently desirable to separate potentially interfering cellular components of the material from the other fluid prior to testing.
- Such measurement and separation steps have previously been typically performed by centrifugation to separate, for instance, blood plasma from the cellular components, followed by manual or automated pipetting of predetermined volumes of the blood plasma into separate test wells.
- centrifugal rotors are designed to measure volumes of a biological fluid, such as blood, remove cellular components, and mix the fluid with an appropriate diluent for analysis, for example by optical testing.
- the rotors provide a plurality of discrete volumes of sample in separate cuvettes in which the sample is optically analyzed. To ensure accurate and consistent results, such rotors require the delivery of precisely measured volumes of liquid to various chambers in the rotor.
- U.S. Patent Nos. 4,894,204, and 5,160,702 disclose siphons for transferring fluids between chambers in a rotor.
- U.S. Patent No. 4,244,916 discloses a rotor comprising a plurality of cuvettes positioned radially outward of a central receptacle. Each cuvette is connected to the central receptacle by a duct and comprises a separate air escape orifice.
- U.S. Patent No. 4,902,479 discloses a multi- cuvette rotor comprising elongated, radially extending cuvettes. Each elongated cuvette comprises a first chamber for receiving a first constituent and a second chamber for receiving a second constituent. A divider structure between the first and second chambers prevents mixing of the constituents before a predetermined time. Mixing occurs as the rotor is spun at a sufficient speed.
- U.S. Patent No. 5,077,013 discloses rotors comprising peripheral cuvettes connected to holding chambers positioned radially inward from the cuvettes.
- the present invention provides centrifugal rotors comprising siphons for delivering a premeasured volume of liquid, typically a biological sample such as plasma, between a first and a second chamber in the rotor.
- the siphons of the invention have an elbow that is radially inward of the radially most inward point of the fluid in the first chamber. As the rotor is spinning the fluid does not flow past the elbow. After the rotor stops, capillary forces "prime" the siphon by pulling fluid just around the elbow.
- the siphons of the invention are designed such that the inlet of the siphon on the first chamber is radially outward of the siphon outlet on the second chamber.
- the positioning of the inlets and outlets of the siphons of the invention provide a number of advantages.
- the inlet of the siphon is always positioned radially outward of the final position of the meniscus of the fluid in the first chamber, after fluid has been transferred to the second chamber.
- inaccuracy in measurement associated with different shaped menisci in different fluids is minimized since the meniscus is minimized.
- all siphons are semi-stable because the train of fluid in a siphon is stable but easily broken if the rotor is perturbed. When the train of fluid is broken, under centrifugal force, the fluid contained in the siphon will flow to the radially most outward point. In prior art siphons this point is the siphon outlet.
- the radially most outward point in the siphon is the siphon inlet.
- the problem of delivering unmetered volumes of fluid is avoided because the fluid flows back into the first chamber when the train of fluid is broken.
- the chambers connected by the siphons of the invention are used to perform any of a number of functions, such as metering liquids, separating solid components from a sample, mixing diluent with the sample, and the like.
- the siphons connect a plasma metering chamber to a mixing chamber for mixing the premeasured volume of plasma with diluent.
- the rotors of the invention comprise unmodified inlet channels connecting a distribution ring to cuvettes comprising reagents for optical analysis of a biological sample.
- the inlet channels are sized such that, as the rotor spins, gas escapes from the cuvette through the inlet channel as the liquid enters the cuvette through the inlet channel.
- an "unmodified inlet channel” as used herein refers to a simple inlet channel, typically having a rectangular cross section, which is not modified (e.g., by altering the cross-sectional shape, surface texture, and the like) to provide a pathway for gas to escape from a cuvette that is not otherwise vented.
- FIGs. 1A-1F are top plan views of a rotor of the invention showing the flow of fluids through the chambers and channels of the rotor as the rotor is spun.
- the present invention provides methods and devices for the delivery of liquids to chambers in an analytical rotor.
- the rotors of the invention comprise siphons which ensure precise delivery of etered volumes of liquid to a desired chamber in the rotor.
- the rotors of the invention are suitable for the analysis of any liquid, typically a biological sample such as whole blood or plasma. It is also useful with numerous other biological fluids, such as urine, sputum, semen, saliva, ocular lens fluid, cerebral fluid, spinal fluid, amniotic fluid. Other fluids that can be tested include tissue culture media, food and industrial chemicals, environmental samples and the like.
- the rotors typically provide chambers which can separate cellular components from the biological sample (e.g. whole blood) , measure a precise volume of liquid sample (e.g. plasma) , mix the sample with an appropriate diluent and deliver the diluted sample to cuvettes for optical analysis.
- the fluid delivered to the cuvettes undergoes reaction(s) within the cuvettes, e.g., reaction with a reagent which forms part of an analytical procedure to detect one or more analytes within the fluid.
- the sample may further be optically analyzed while present in the rotor, either with or without prior reaction.
- the apparatus of the present invention comprises an analytical rotor having a rotor body which is capable of being mounted on a conventional laboratory centrifuge of the type which is commercially available from suppliers, such a Beckman Instruments, Inc., Spinco Division, Fullerton, California; Fisher Scientific, Pittsburgh, Pennsylvania; VWR Scientific, San Francisco, California, and the like.
- the centrifugal rotor will include a receptacle or other coupling device suitable for mounting on a vertical drive shaft provided by the centrifuge.
- the particular design of the receptacle or coupling device will depend on the nature of the centrifuge, and it will be appreciated that the centrifugal rotor of the present invention may be adapted for use with all or most types of centrifuges which are now available or which may become available in the future.
- the rotor body comprises a structure which maintains a desired geometric pattern or relationship between a plurality of chambers, interconnecting passages, and vents, as described in more detail below.
- Various specialized chambers and channels suitable for use in the rotors of the invention are disclosed in U.S. Patent Nos. 5,061,381 and 5,122,284, and U.S.S.N. 07/678,762 and 07/783,041 which are incorporated herein by reference.
- the body will be a substantially solid plate or disk with the chambers and passages formed as spaces or voids in the otherwise solid matrix.
- such solid plate structures may be formed by laminating a plurality of separately-formed layers together into a composite structure where the chambers and horizontal passages are generally formed between adjacent layers.
- the vertical passages may be formed through the layers.
- the individual layers may be formed by injection molding, machining, or combinations thereof, and will usually be joined together, typically using a suitable adhesive or by ultrasonic welding. The final enclosed volumes are formed when the layers are brought together.
- centrifugal rotor could also be formed as a plurality of discrete components, such as tubes, vessels, chambers, etc., arranged in a suitable framework.
- the rotor body may be formed from a wide variety of materials and may optionally include two or more materials. Usually, the material(s) will be transparent so that the presence and distribution of the biological fluid, cellular components, and reagents, may be observed within the various internal chambers and passages.
- analytical chambers e.g., cuvettes, or other test wells are formed within the rotor
- suitable optical paths formed within the rotor so that the contents of the cuvettes may be observed spectrophotometrically, fluorometrically, or by other optical assessment instruments.
- suitable cuvettes having particular optical paths formed therethrough is disclosed in U.S. Patent No. 5,173,193, the disclosure of which is incorporated herein by reference.
- the rotor is formed with an acrylic resin having suitable optical properties, at least in those areas which define an optical path.
- the apparatus and method of the present invention are suitable for performing a wide variety of analytic procedures and assays which are beneficially or necessarily performed on blood plasma and other samples.
- the analytic procedures may require that the sample be combined with one or more reagents so that some detectable change occurs which may be related to the presence and/or amount of a particular component (analyte) or characteristic of the sample.
- the sample may undergo a reaction or other change which results in a change in color, fluorescence, luminescence, or the like, which may be measured by conventional spectrophotometers, fluorometers, light detectors, and the like.
- immunoassays and other specific binding assays may be performed within the cell-free fluid collection chamber or within cuvettes which are connected to the collection chamber.
- such assay procedures should be homogeneous and not require a separation step.
- it may be possible to accommodate heterogeneous assay systems by providing a means to separate the sample (e.g., blood plasma) from the collection chamber or another test well or cuvette after the immunological reaction step has occurred.
- the means of analyzing the sample is not an important aspect of the invention. Any of a number of analytical methods can be adapted for use in the rotors of the invention, depending upon the particular sample being analyzed and component being detected.
- assays In the case of blood analyses, conventional blood assays are typically preformed. Examples of assays which may be performed include those designed to detect glucose, lactate dehydrogenase, serum glutamic-oxaloacetic transaminase (SGOT) , serum glutamic-pyruvic transaminase (SGPT) , blood urea nitrogen (BUN) , total protein, alkalinity, phosphatase, bilirubin, calcium, chloride, sodium, potassium, magnesium, and the like. This list is not exhaustive and is intended merely as being exemplary of the assays which may be performed using the apparatus and method of the present invention.
- these tests will require that the blood and plasma be combined with one or more reagents which result in an optically detectable, usually photometrically detectable, change in the plasma.
- the reagents which are required are well known and amply described in the patent and scientific literature.
- the reagents are preferably provided in lyophilized form to increase stability. Ideally, they are provided in the form of lyophilized reagent spheres as described in U.S.S.N. 07/747,179, which is incorporated herein by reference.
- Figure 1A shows the position of a blood sample 102 in the blood application chamber 104 after the sample has been loaded in the rotor body 100.
- a diluent container in chamber 106 is opened upon mounting of the rotor on the spindle of the centrifuge as described in copending and commonly assigned application, U.S. Serial No. 07/873,327, which is incorporated herein by reference.
- Fig. IB shows the position of the diluent 108 and blood sample 102 after the rotor is spun at 4,000 rpm.
- the blood sample 102 begins to exit the blood application chamber 104 and enters the plasma metering chamber 110.
- diluent 108 empties from the diluent container into the holding chamber 112.
- the diluent immediately begins to enter the diluent metering chamber 114 through channel 116.
- Fig. 1C shows the position of the liquids as the rotor 100 continues to spin.
- the blood sample 102 has emptied the blood application chamber 104 and overflows the plasma metering chamber 110 into the overflow chamber 118 where it flows to the hemoglobin cuvette 120 and the excess blood dump 122.
- diluent 108 fills the diluent metering chamber 114 and excess flows through channel 124 to diluent-only cuvettes 126 and excess diluent dump 127.
- Fig. ID shows the position of the liquids at the conclusion of the first spin.
- the blood sample 102 has separated into cells 128 and plasma 130.
- the diluent-only cuvettes 126 are filled and a predetermined amount of diluent remains in the diluent metering chamber 114.
- the rotor 100 is then stopped and the siphon 132 from the diluent metering chamber 114, as well as the siphon 134 from the plasma metering chamber 110, are allowed to prime, as described above.
- Siphon 134 is a siphon of the present invention. It is connected to the plasma metering chamber 110 at inlet 138.
- the inlet 138 is position radially outward of the siphon outlet 139, through which the siphon 134 empties into the mixing chamber 136.
- Fig. IE shows the position of the liquids during the second spin of the rotor.
- the diluent metering chamber 114 empties into the mixing chamber 136 through siphon 132.
- a predetermined amount of plasma 130 is metered into the mixing chamber 136 and the two fluids are mixed, thereby forming diluted plasma 131.
- the amount of plasma 130 delivered to the mixing chamber 136 is determined by the position of the outlet 139 on the siphon 134.
- the final level of the plasma 133 in the plasma metering chamber 110 is at the same radial position as the outlet 139.
- the volume of plasma delievered to the mixing chamber 136 is determined by the volume of the plasma metering chamber 110 between the exit to the overflow chamber 129 and the final level of plasma 133.
- Fig. IF shows the position of the diluted plasma 131 as the rotor is spun during the third spin. This figure illustrates the movement of the diluted plasma 131 through the distribution ring 142 and inlet channels 144 to the cuvettes 146 and excess plasma dump 147.
- the resistance to flow in the output siphon 140 is selected to be higher than the resistance to flow in the distribution ring 142 and the inlet channels 144 so that air present in the cuvettes 146 can escape as the cuvettes are filled.
- siphon 140 is dimensioned such that the ratio of the cross sectional area of the inlet channels 144 to the cross sectional area of the liquid in them is greater than 2:1, preferably greater than about 4:1.
- the cross sectional area of the inlet channels 144 is typically the same as or slightly smaller than that of the distribution channel 142 so that gas in the unvented cuvettes escapes through the inlet channels 144 and distribution 142. If the sample is plasma or diluted plasma and the channels are rectangular in cross-section, their dimensions are typically as follows: siphon: 0.150 mm depth, 0.200 mm width; distribution channel 0.300 mm depth, 0.500mm width; inlet channels: 0.150 depth, 0.500 width.
Landscapes
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Centrifugal Separators (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Optical Measuring Cells (AREA)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002192196A CA2192196C (en) | 1994-06-06 | 1995-06-05 | Modified siphons for improved metering precision |
JP8501264A JPH10501340A (ja) | 1994-06-06 | 1995-06-05 | 測定精度を改善するための改良サイホン |
EP95922222A EP0764266A4 (en) | 1994-06-06 | 1995-06-05 | MODIFIED SIPHONE TO IMPROVE DOSING ACCURACY |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25440694A | 1994-06-06 | 1994-06-06 | |
US08/254,406 | 1994-06-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995033986A1 true WO1995033986A1 (en) | 1995-12-14 |
Family
ID=22964194
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1995/007145 WO1995033986A1 (en) | 1994-06-06 | 1995-06-05 | Modified siphons for improved metering precision |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0764266A4 (ja) |
JP (3) | JPH10501340A (ja) |
CA (1) | CA2192196C (ja) |
WO (1) | WO1995033986A1 (ja) |
Cited By (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5627041A (en) * | 1994-09-02 | 1997-05-06 | Biometric Imaging, Inc. | Disposable cartridge for an assay of a biological sample |
US6143247A (en) * | 1996-12-20 | 2000-11-07 | Gamera Bioscience Inc. | Affinity binding-based system for detecting particulates in a fluid |
US6143248A (en) * | 1996-08-12 | 2000-11-07 | Gamera Bioscience Corp. | Capillary microvalve |
US6302134B1 (en) | 1997-05-23 | 2001-10-16 | Tecan Boston | Device and method for using centripetal acceleration to device fluid movement on a microfluidics system |
WO2002043866A2 (en) * | 2000-12-01 | 2002-06-06 | Burstein Technologies, Inc. | Apparatus and methods for separating components of particulate suspension |
US6632399B1 (en) | 1998-05-22 | 2003-10-14 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
US6709869B2 (en) | 1995-12-18 | 2004-03-23 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
US6808633B1 (en) | 1999-06-23 | 2004-10-26 | Hitachi, Ltd. | Centrifugal separator and sample preparation device using the separator |
EP1503209A1 (en) * | 2002-05-08 | 2005-02-02 | Hitachi High-Technologies Corporation | Chemical analyzer and gene diagnosing apparatus |
US6919058B2 (en) | 2001-08-28 | 2005-07-19 | Gyros Ab | Retaining microfluidic microcavity and other microfluidic structures |
EP1600778A1 (en) * | 2003-02-19 | 2005-11-30 | Japan Science and Technology Agency | Blood analysis device and blood analysis method |
US7033747B2 (en) | 2001-04-11 | 2006-04-25 | Nagaoka & Co., Ltd | Multi-parameter assays including analysis discs and methods relating thereto |
US7054258B2 (en) | 2000-12-08 | 2006-05-30 | Nagaoka & Co., Ltd. | Optical disc assemblies for performing assays |
US7079468B2 (en) | 2000-12-08 | 2006-07-18 | Burstein Technologies, Inc. | Optical discs for measuring analytes |
US7091034B2 (en) | 2000-12-15 | 2006-08-15 | Burstein Technologies, Inc. | Detection system for disk-based laboratory and improved optical bio-disc including same |
US7189368B2 (en) | 2001-09-17 | 2007-03-13 | Gyros Patent Ab | Functional unit enabling controlled flow in a microfluidic device |
DE102005048260A1 (de) * | 2005-10-07 | 2007-04-12 | Albert-Ludwigs-Universität Freiburg | Verfahren und Vorrichtung zum Handhaben einer flüssigen Probe unter Verwendung einer Rotation mit einem zeitlich veränderlichen Drehvektor |
DE102005048233A1 (de) * | 2005-10-07 | 2007-04-12 | Albert-Ludwigs-Universität Freiburg | Vorrichtung und Verfahren zum Handhaben einer flüssigen Probe unter Verwendung einer Siphon-Struktur |
US7384602B2 (en) | 2002-05-08 | 2008-06-10 | Hitachi High-Technologies Corporation | Chemical analysis apparatus and genetic diagnostic apparatus |
US7390464B2 (en) | 2003-06-19 | 2008-06-24 | Burstein Technologies, Inc. | Fluidic circuits for sample preparation including bio-discs and methods relating thereto |
US7429354B2 (en) | 2001-03-19 | 2008-09-30 | Gyros Patent Ab | Structural units that define fluidic functions |
EP1985366A3 (en) * | 2007-04-24 | 2009-04-22 | Samsung Electronics Co., Ltd. | Centrifugal microfluidic device having sample distribution structure and centrifugal microfluidic system including the centrifugal microfluidic device |
EP2072131A1 (de) * | 2007-12-13 | 2009-06-24 | Roche Diagnostics GmbH | Mikrofluidisches Element zur Durchmischung einer Flüssigkeit in einer Reagenz |
EP2126119A1 (en) * | 2007-03-02 | 2009-12-02 | Corbett Research Pty Ltd | Apparatus and method for nucleic acid amplification |
US7749442B2 (en) | 2001-12-14 | 2010-07-06 | Arkray, Inc. | Sample measuring device |
WO2010077159A1 (en) * | 2008-12-30 | 2010-07-08 | Biosurfit, S.A. | Analytical rotors and methods for analysis of biological fluids |
US7850909B2 (en) | 2002-09-26 | 2010-12-14 | Arkray, Inc. | Analytical tool |
EP2269736A1 (en) | 2001-08-28 | 2011-01-05 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
WO2012055707A1 (de) | 2010-10-28 | 2012-05-03 | Roche Diagnostics Gmbh | Mikrofluidischer testträger zum aufteilen einer flüssigkeitsmenge in teilmengen |
WO2012055861A1 (de) | 2010-10-29 | 2012-05-03 | Roche Diagnostics Gmbh | Mikrofluidisches element zur analyse einer probenflüssigkeit |
US8303908B2 (en) | 2002-10-28 | 2012-11-06 | Arkray, Inc. | Analyzing tool and device |
WO2012158990A1 (en) * | 2011-05-18 | 2012-11-22 | 3M Innovative Properties Company | Systems and methods for volumetric metering on a sample processing device |
US8596150B2 (en) | 2009-03-12 | 2013-12-03 | Panasonic Corporation | Analytical device with mixing cavity |
US8865005B2 (en) | 2008-10-23 | 2014-10-21 | Biosurfit, S.A. | Jet deflection device |
US8916112B2 (en) | 2010-03-29 | 2014-12-23 | Biosurfit, S.A. | Liquid distribution and metering |
US9013704B2 (en) | 2009-12-22 | 2015-04-21 | Biosurfit, S.A. | Surface plasmon resonance detection system |
US9046503B2 (en) | 2008-07-17 | 2015-06-02 | Panasonic Healthcare Co., Ltd. | Analyzing device |
US9067205B2 (en) | 2011-05-18 | 2015-06-30 | 3M Innovative Properties Company | Systems and methods for valving on a sample processing device |
US9145579B2 (en) | 2012-07-24 | 2015-09-29 | Panasonic Healthcare Co., Ltd. | Analyzing device |
US9168523B2 (en) | 2011-05-18 | 2015-10-27 | 3M Innovative Properties Company | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
US9562262B2 (en) | 2011-03-08 | 2017-02-07 | UNIVERSITé LAVAL | Fluidic centripetal device |
USD799715S1 (en) | 2015-10-23 | 2017-10-10 | Gene POC, Inc. | Fluidic centripetal device |
US9933348B2 (en) | 2011-12-08 | 2018-04-03 | Biosurfit, S.A. | Sequential aliqoting and determination of an indicator of sedimentation rate |
US10620194B2 (en) | 2001-03-19 | 2020-04-14 | Gyros Patent Ab | Characterization of reaction variables |
US20200238279A1 (en) * | 2017-03-08 | 2020-07-30 | Northwestern University | Devices, systems, and methods for specimen preparation and analysis using capillary and centrifugal forces |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60035199T2 (de) | 1999-08-11 | 2008-02-14 | Asahi Kasei Kabushiki Kaisha | Analysenkassette und flüssigkeitsförderkontroller |
US7662340B2 (en) * | 2001-12-28 | 2010-02-16 | Hitachi High-Technologies Corporation | Extractor chemical analyzer and chemical analyzing method |
JP4619224B2 (ja) * | 2005-07-27 | 2011-01-26 | パナソニック株式会社 | 回転分析デバイス |
JP4531677B2 (ja) * | 2005-10-24 | 2010-08-25 | パナソニック株式会社 | アッセイ方法、それを実施するためのセンサデバイス、およびセンサデバイス用測定装置 |
US20090169430A1 (en) * | 2006-04-04 | 2009-07-02 | Matsushita Electric Industrial Co., Ltd. | Panel for analyzing sample liquid |
US8277759B2 (en) | 2008-03-04 | 2012-10-02 | The University Of Utah Research Foundation | Microfluidic flow cell |
KR101099495B1 (ko) * | 2008-10-14 | 2011-12-28 | 삼성전자주식회사 | 원심력 기반의 미세유동장치, 이의 제조 방법 및 이를 이용한 시료분석방법 |
ES2755490T3 (es) | 2013-04-15 | 2020-04-22 | Becton Dickinson Co | Dispositivo de extracción de fluidos biológicos y sistema de separación de fluidos biológicos |
MX2018016334A (es) * | 2016-06-27 | 2019-09-18 | Abay Sa | Dispositivo con conductos modificados. |
CN112169853B (zh) * | 2020-12-01 | 2021-02-26 | 南京岚煜生物科技有限公司 | 一种多功能微流控检测芯片 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4743558A (en) * | 1984-10-26 | 1988-05-10 | Jean Guigan | Method of performing medical analysis on a sample of liquid by means of at least one liquid reagent, and apparatus for performing the method |
US4894204A (en) * | 1984-12-21 | 1990-01-16 | Inovelf | Rotor with dynamic pipeting for a centrifuge analysis device |
US4999304A (en) * | 1987-12-28 | 1991-03-12 | Miles Inc. | Dynamic braking centrifuge |
US5256376A (en) * | 1991-09-12 | 1993-10-26 | Medical Laboratory Automation, Inc. | Agglutination detection apparatus |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2579756B1 (fr) * | 1985-03-26 | 1987-05-07 | Guigan Jean | Procede pour realiser des analyses biologiques utilisant des reactions immunologiques et dispositif de mise en oeuvre |
US5160702A (en) * | 1989-01-17 | 1992-11-03 | Molecular Devices Corporation | Analyzer with improved rotor structure |
US5304348A (en) * | 1992-02-11 | 1994-04-19 | Abaxis, Inc. | Reagent container for analytical rotor |
AU4047493A (en) * | 1992-04-02 | 1993-11-08 | Abaxis, Inc. | Analytical rotor with dye mixing chamber |
US5591643A (en) | 1993-09-01 | 1997-01-07 | Abaxis, Inc. | Simplified inlet channels |
-
1995
- 1995-06-05 CA CA002192196A patent/CA2192196C/en not_active Expired - Lifetime
- 1995-06-05 JP JP8501264A patent/JPH10501340A/ja active Pending
- 1995-06-05 WO PCT/US1995/007145 patent/WO1995033986A1/en active Application Filing
- 1995-06-05 EP EP95922222A patent/EP0764266A4/en not_active Withdrawn
-
2006
- 2006-08-17 JP JP2006222178A patent/JP4178169B2/ja not_active Expired - Lifetime
-
2008
- 2008-01-25 JP JP2008014351A patent/JP2008157960A/ja active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4743558A (en) * | 1984-10-26 | 1988-05-10 | Jean Guigan | Method of performing medical analysis on a sample of liquid by means of at least one liquid reagent, and apparatus for performing the method |
US4894204A (en) * | 1984-12-21 | 1990-01-16 | Inovelf | Rotor with dynamic pipeting for a centrifuge analysis device |
US4999304A (en) * | 1987-12-28 | 1991-03-12 | Miles Inc. | Dynamic braking centrifuge |
US5256376A (en) * | 1991-09-12 | 1993-10-26 | Medical Laboratory Automation, Inc. | Agglutination detection apparatus |
Non-Patent Citations (1)
Title |
---|
See also references of EP0764266A4 * |
Cited By (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5627041A (en) * | 1994-09-02 | 1997-05-06 | Biometric Imaging, Inc. | Disposable cartridge for an assay of a biological sample |
US6319468B1 (en) | 1995-06-27 | 2001-11-20 | Tecan Trading Ag | Affinity binding-based system for detecting particulates in a fluid |
US6709869B2 (en) | 1995-12-18 | 2004-03-23 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
US6143248A (en) * | 1996-08-12 | 2000-11-07 | Gamera Bioscience Corp. | Capillary microvalve |
US6143247A (en) * | 1996-12-20 | 2000-11-07 | Gamera Bioscience Inc. | Affinity binding-based system for detecting particulates in a fluid |
US6656430B2 (en) | 1996-12-20 | 2003-12-02 | Tecan Trading Ag | Affinity binding-based system for detecting particulates in a fluid |
US6302134B1 (en) | 1997-05-23 | 2001-10-16 | Tecan Boston | Device and method for using centripetal acceleration to device fluid movement on a microfluidics system |
US6399361B2 (en) | 1997-05-23 | 2002-06-04 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
US6548788B2 (en) | 1997-05-23 | 2003-04-15 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system |
US6632399B1 (en) | 1998-05-22 | 2003-10-14 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
US6808633B1 (en) | 1999-06-23 | 2004-10-26 | Hitachi, Ltd. | Centrifugal separator and sample preparation device using the separator |
WO2002043866A3 (en) * | 2000-12-01 | 2003-03-06 | Burstein Technologies Inc | Apparatus and methods for separating components of particulate suspension |
WO2002043866A2 (en) * | 2000-12-01 | 2002-06-06 | Burstein Technologies, Inc. | Apparatus and methods for separating components of particulate suspension |
US7054258B2 (en) | 2000-12-08 | 2006-05-30 | Nagaoka & Co., Ltd. | Optical disc assemblies for performing assays |
US7889615B2 (en) | 2000-12-08 | 2011-02-15 | Vindur Technologies, Inc. | Optical discs for measuring analytes |
US7599275B2 (en) | 2000-12-08 | 2009-10-06 | Vindur Technologies, Inc. | Optical discs for measuring analytes |
US7542383B2 (en) | 2000-12-08 | 2009-06-02 | Vindur Technologies, Inc. | Optical disc assemblies for performing assays |
US7366063B2 (en) | 2000-12-08 | 2008-04-29 | Burstein Technologies, Inc. | Optical discs for measuring analytes |
US7200100B2 (en) | 2000-12-08 | 2007-04-03 | Nagaoka & Co., Ltd. | Optical disc assemblies for performing assays |
US7079468B2 (en) | 2000-12-08 | 2006-07-18 | Burstein Technologies, Inc. | Optical discs for measuring analytes |
US7091034B2 (en) | 2000-12-15 | 2006-08-15 | Burstein Technologies, Inc. | Detection system for disk-based laboratory and improved optical bio-disc including same |
US7429354B2 (en) | 2001-03-19 | 2008-09-30 | Gyros Patent Ab | Structural units that define fluidic functions |
US10620194B2 (en) | 2001-03-19 | 2020-04-14 | Gyros Patent Ab | Characterization of reaction variables |
US7033747B2 (en) | 2001-04-11 | 2006-04-25 | Nagaoka & Co., Ltd | Multi-parameter assays including analysis discs and methods relating thereto |
US7300199B2 (en) | 2001-08-28 | 2007-11-27 | Gyros Ab | Retaining microfluidic microcavity and other microfluidic structures |
US7275858B2 (en) | 2001-08-28 | 2007-10-02 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
EP2283924A1 (en) | 2001-08-28 | 2011-02-16 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
US6919058B2 (en) | 2001-08-28 | 2005-07-19 | Gyros Ab | Retaining microfluidic microcavity and other microfluidic structures |
EP2281633A1 (en) | 2001-08-28 | 2011-02-09 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
US7459129B2 (en) | 2001-08-28 | 2008-12-02 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
EP2269736A1 (en) | 2001-08-28 | 2011-01-05 | Gyros Patent Ab | Retaining microfluidic microcavity and other microfluidic structures |
US7189368B2 (en) | 2001-09-17 | 2007-03-13 | Gyros Patent Ab | Functional unit enabling controlled flow in a microfluidic device |
US7749442B2 (en) | 2001-12-14 | 2010-07-06 | Arkray, Inc. | Sample measuring device |
EP1503209A4 (en) * | 2002-05-08 | 2005-07-06 | Hitachi High Tech Corp | CHEMICAL ANALYZER AND GENE DIAGNOSTIC APPARATUS |
EP1503209A1 (en) * | 2002-05-08 | 2005-02-02 | Hitachi High-Technologies Corporation | Chemical analyzer and gene diagnosing apparatus |
US7384602B2 (en) | 2002-05-08 | 2008-06-10 | Hitachi High-Technologies Corporation | Chemical analysis apparatus and genetic diagnostic apparatus |
US7850909B2 (en) | 2002-09-26 | 2010-12-14 | Arkray, Inc. | Analytical tool |
US8303908B2 (en) | 2002-10-28 | 2012-11-06 | Arkray, Inc. | Analyzing tool and device |
EP1600778A4 (en) * | 2003-02-19 | 2012-08-08 | Japan Science & Tech Agency | BLOOD ANALYSIS DEVICE AND BLOOD ANALYSIS METHOD |
EP1600778A1 (en) * | 2003-02-19 | 2005-11-30 | Japan Science and Technology Agency | Blood analysis device and blood analysis method |
US7390464B2 (en) | 2003-06-19 | 2008-06-24 | Burstein Technologies, Inc. | Fluidic circuits for sample preparation including bio-discs and methods relating thereto |
DE102005048233A1 (de) * | 2005-10-07 | 2007-04-12 | Albert-Ludwigs-Universität Freiburg | Vorrichtung und Verfahren zum Handhaben einer flüssigen Probe unter Verwendung einer Siphon-Struktur |
DE102005048260A1 (de) * | 2005-10-07 | 2007-04-12 | Albert-Ludwigs-Universität Freiburg | Verfahren und Vorrichtung zum Handhaben einer flüssigen Probe unter Verwendung einer Rotation mit einem zeitlich veränderlichen Drehvektor |
EP2126119A4 (en) * | 2007-03-02 | 2014-07-16 | Corbett Res Pty Ltd | APPARATUS AND METHOD FOR NUCLEIC ACID AMPLIFICATION |
EP2126119A1 (en) * | 2007-03-02 | 2009-12-02 | Corbett Research Pty Ltd | Apparatus and method for nucleic acid amplification |
US8048387B2 (en) | 2007-04-24 | 2011-11-01 | Samsung Electronics Co., Ltd. | Centrifugal microfluidic device having sample distribution structure and centrifugal microfluidic system including the centrifugal microfluidic device |
EP2420319A1 (en) * | 2007-04-24 | 2012-02-22 | Samsung Electronics Co., Ltd | Centrifugal microfluidic device having sample distribution structure and centrifugal microfluidic system including the centrifugal microfluidic device |
EP1985366A3 (en) * | 2007-04-24 | 2009-04-22 | Samsung Electronics Co., Ltd. | Centrifugal microfluidic device having sample distribution structure and centrifugal microfluidic system including the centrifugal microfluidic device |
US10031053B2 (en) | 2007-12-13 | 2018-07-24 | Roche Diagnostics Operations, Inc | Microfluidic element for thoroughly mixing a liquid with a reagent |
US9417164B2 (en) | 2007-12-13 | 2016-08-16 | Roche Diagnostics Operations, Inc. | Microfluidic element for thoroughly mixing a liquid with a reagent |
EP2072131A1 (de) * | 2007-12-13 | 2009-06-24 | Roche Diagnostics GmbH | Mikrofluidisches Element zur Durchmischung einer Flüssigkeit in einer Reagenz |
US9962700B2 (en) | 2008-07-17 | 2018-05-08 | Panasonic Healthcare Holdings Co., Ltd. | Analyzing device |
US9046503B2 (en) | 2008-07-17 | 2015-06-02 | Panasonic Healthcare Co., Ltd. | Analyzing device |
US8865005B2 (en) | 2008-10-23 | 2014-10-21 | Biosurfit, S.A. | Jet deflection device |
WO2010077159A1 (en) * | 2008-12-30 | 2010-07-08 | Biosurfit, S.A. | Analytical rotors and methods for analysis of biological fluids |
US8440147B2 (en) | 2008-12-30 | 2013-05-14 | Biosurfit, S.A. | Analytical rotors and methods for analysis of biological fluids |
US8596150B2 (en) | 2009-03-12 | 2013-12-03 | Panasonic Corporation | Analytical device with mixing cavity |
US9013704B2 (en) | 2009-12-22 | 2015-04-21 | Biosurfit, S.A. | Surface plasmon resonance detection system |
US8916112B2 (en) | 2010-03-29 | 2014-12-23 | Biosurfit, S.A. | Liquid distribution and metering |
US9186671B2 (en) | 2010-10-28 | 2015-11-17 | Roche Diagnostics Operations, Inc. | Microfluidic test carrier for apportioning a liquid quantity into subquantities |
WO2012055707A1 (de) | 2010-10-28 | 2012-05-03 | Roche Diagnostics Gmbh | Mikrofluidischer testträger zum aufteilen einer flüssigkeitsmenge in teilmengen |
EP2486978A1 (de) | 2010-10-28 | 2012-08-15 | Roche Diagnostics GmbH | Mikrofluidischer Testträger zum Aufteilen einer Flüssigkeitsmenge in Teilmengen |
US9221051B2 (en) | 2010-10-29 | 2015-12-29 | Roche Diagnostics Operations, Inc. | Microfluidic element for analysis of a sample liquid |
WO2012055861A1 (de) | 2010-10-29 | 2012-05-03 | Roche Diagnostics Gmbh | Mikrofluidisches element zur analyse einer probenflüssigkeit |
EP2455162A1 (de) | 2010-10-29 | 2012-05-23 | Roche Diagnostics GmbH | Mikrofluidisches Element zur Analyse einer Probenflüssigkeit |
US9562262B2 (en) | 2011-03-08 | 2017-02-07 | UNIVERSITé LAVAL | Fluidic centripetal device |
US11123730B2 (en) | 2011-03-08 | 2021-09-21 | Universite Laval | Fluidic centripetal device |
US10427158B2 (en) | 2011-03-08 | 2019-10-01 | UNIVERSITé LAVAL | Fluidic centripetal device |
US8931331B2 (en) | 2011-05-18 | 2015-01-13 | 3M Innovative Properties Company | Systems and methods for volumetric metering on a sample processing device |
US9725762B2 (en) | 2011-05-18 | 2017-08-08 | Diasorin S.P.A. | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
WO2012158990A1 (en) * | 2011-05-18 | 2012-11-22 | 3M Innovative Properties Company | Systems and methods for volumetric metering on a sample processing device |
US9067205B2 (en) | 2011-05-18 | 2015-06-30 | 3M Innovative Properties Company | Systems and methods for valving on a sample processing device |
CN103547370A (zh) * | 2011-05-18 | 2014-01-29 | 3M创新有限公司 | 用于样品处理装置上体积计量的系统和方法 |
US9168523B2 (en) | 2011-05-18 | 2015-10-27 | 3M Innovative Properties Company | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
US9933348B2 (en) | 2011-12-08 | 2018-04-03 | Biosurfit, S.A. | Sequential aliqoting and determination of an indicator of sedimentation rate |
US9145579B2 (en) | 2012-07-24 | 2015-09-29 | Panasonic Healthcare Co., Ltd. | Analyzing device |
USD799715S1 (en) | 2015-10-23 | 2017-10-10 | Gene POC, Inc. | Fluidic centripetal device |
US20200238279A1 (en) * | 2017-03-08 | 2020-07-30 | Northwestern University | Devices, systems, and methods for specimen preparation and analysis using capillary and centrifugal forces |
Also Published As
Publication number | Publication date |
---|---|
EP0764266A4 (en) | 1998-08-05 |
CA2192196C (en) | 2004-11-23 |
JPH10501340A (ja) | 1998-02-03 |
JP2008157960A (ja) | 2008-07-10 |
JP4178169B2 (ja) | 2008-11-12 |
CA2192196A1 (en) | 1995-12-14 |
EP0764266A1 (en) | 1997-03-26 |
JP2006317467A (ja) | 2006-11-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6235531B1 (en) | Modified siphons for improved metering precision | |
CA2192196C (en) | Modified siphons for improved metering precision | |
US5591643A (en) | Simplified inlet channels | |
US5409665A (en) | Simultaneous cuvette filling with means to isolate cuvettes | |
US5173193A (en) | Centrifugal rotor having flow partition | |
CA2129967C (en) | Reagent container for analytical rotor | |
US5186844A (en) | Apparatus and method for continuous centrifugal blood cell separation | |
US5122284A (en) | Apparatus and method for optically analyzing biological fluids | |
CA2082827C (en) | Analytical rotors and methods for analysis of biological fluids | |
US5472603A (en) | Analytical rotor with dye mixing chamber | |
US5061381A (en) | Apparatus and method for separating cells from biological fluids | |
US5242606A (en) | Sample metering port for analytical rotor having overflow chamber | |
JP4707035B2 (ja) | マイクロ流体装置における混合 | |
CA2347669C (en) | Analytical rotors and methods for analysis of biological fluids | |
NZ749465A (en) | Devices with modified conduits |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): CA JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2192196 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1995922222 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1995922222 Country of ref document: EP |