WO1995017938A1

WO1995017938A1 - Device for obtaining sediment and supernatant from structural biological fluids of low cellular structure under the effect of centrifugal forces

Info

Publication number: WO1995017938A1
Application number: PCT/PL1994/000006
Authority: WO
Inventors: Tadeusz Gaszczynski; Pawe$m(D) RÓZYCKI; Dariusz Bednarczyk; Waldemar Pakszys
Original assignee: MECHANIKA PRECYZYJNA SPÓ$m(C)DZIELNIA PRACY
Priority date: 1993-12-28
Filing date: 1994-04-20
Publication date: 1995-07-06
Also published as: PL301657A1; PL173226B1

Abstract

The subject of the invention relates to the device for obtaining the sediment and the supernatant from low cellular structural biological fluids under the effect of the centrifugal forces. The described device is used especially for the testing of morphotic cerebro-spinal fluids. The frame consists of the monolithic upper part (1) with the working channel connected in the lower part (3) with the overflow channel (7), the symmetrical axes of which make up the angle α=15 30, while their narrowing ends (12 and 13) have snap locks (14) and in the lower part, the packing (25); and of the monolithic power part (15) with the recesses (16 and 27), the borders (17 and 28) of which are the basis for the lower surface of the microscopic slide (6).

Description

DEVICE FOR OBTAINING SEDIMENT AND SUPERNATANT FROM STRUCTURAL BIOLOGICAL FLUIDS OF LOW CELLULAR STRUCTURE UNDER THE EFFECT OF CENTRIFUGAL FORCES

The subject of the invention is the device for obtaining the sediment and the supernatant from structural biological fluids of a low cellular structure under the effect of the centrifugal forces. This device permits to separate the fluid -from the elements, which are to be microscopically tested. This device is chiefly used for testing the morphotic cerebro-spinal fluid.

The Czeck patent No. 132 057 describes the device for testing morphotic elements. In this device, in addition to the centrifugal separation, the sedimentation is performed by filtering fluids through a hirsute fissure on the filter paper. The device is very complex and its operation causes considerable quantitative losses in morphotic elements which remain on the used filter paper.

The Polish patent No. 86 464 describes the device for testing structural biological fluids of a low cellular structure. This installation has a frame with one or more tanks that are closed from below by microscopic slides which can be moved in and out through the fissure. The slides are tightened by replaceable packings by pressing the sleeves, which are permanent part of the frame, with thread locking rings.

The Polish patent No. P 293341 describes the device for obtaining a fine sediment and a supernatant from biological and technical fluids. In this device, the bottom of the working tank has an overflow opening which is located on the lowest possible level. Parallel with it, under the working tank, there is an oblong channel with a sliding tank for collecting a supernatant. This channel has an in-hole in the upper wall which is shifted toward the overflow opening and toward the axis of the centrifugal separator. The oblong channel has a protrusion on which a sliding tank -- with its thick bottom and a projecting cork, located in the grooves of the outer surface of the sliding tank -- runs. The device has a complex structure which includes sliding and fitting elements. Due to this complex structure, the assemblage of the device and the protection of its bacteriological purity are considerably complicated procedures.

The aim of the invention is the elimination of the shortcomings of the known devices by creating a device which can be easily assembled and operated. The device for obtaining the sediment and the supernatant from structural biological fluids of a low cellular structure under the effect of the centrifugal forces consists of a monolithic upper part with an axial working channel over the ring-shaped recess close to the upper surface of the microscopic slide. This axial working channel is connected below with the overflow channel, while their axes make the angle © = 15 r 30 . The outer surface of the working channel and the outer surface of the overflow channel have narrowing ends with a snap lock. The monolithic lower part has a circular axial outer recess, the borders of which make the basis for the lower surface of the microscopic slide, while the upper lateral surfaces are connected by elastic elements with spring detents. There are outer ribs and the extended mount around the working channel. Two of these ribs are placed symmetrically and make up lateral closing brackets with upper wavy surfaces. There is a packing in the ring-shaped recess, the lower surface of which is located on a microscopic slide. In the monolithic lower part, there is a rectangular recess, the borders of which are parallel with the edges of the lateral walls and on which the lower surface of the microscopic slide is located. According to the invention, the advantage of the described device is, that it makes possible:

-- to eliminate the quantitative and qualitative losses of the structural biological fluids that are being tested;

-- to withdraw the entire supernatant which had been extracted from the morphotic elements by the centrifugal separation;

-- to obtain the sediment directly on the microscopic slide.

The subject of the invention is depicted on the accompanying drawings.

Figure 1 shows a longitudinal axial section of the device, demonstrating the reciprocal location of the working channel and the overflow channel with the pouring device attached to its outer surface in the monolithic upper part; and the location of the packing and the microscopical slide on the borders of recesses in the monolithic lower part.

Figure 2 depicts the longitudinal section of the monolithic upper part of the device with the working channel and the overflow channel, and the ring-shaped recess for the packing; and the profile of the lateral ribs.

Figure 3 is the view of the monolithic upper part from the side of the working channel showing the extended mount and the lateral closing brackets with their upper wavy surfaces .

Figure 4 is the view from the top of the monolithic lower part with the spring detents and the borders of the circular axial and rectangular recesses on which the microscopic slide is located.

Figure 5 is the half-view and the half-section of the

5 lateral projection of the monolithic lower part, showing the height of the borders of the recesses and the connection of the lateral surfaces by elastic elements with the spring detents.

According to the invention, the device consists of

10 the monolithic upper part 1 and the monolithic lower part 15. There is a microscopic slide 6 between them. The monolithic upper part 1 has the axial working channel 2, which is connected in the lower part 3, over the ring- shaped recess 4 and close to the upper surface 5 of the

15 microscopic slide 6, with the overflow channel 7.

Symmetrical axes 8 and 9 of the working channel 2 and the overflow channel 7 make up the angle o = 15 -- 30 . The outer surface 10 of the working channel 2 and the outer surface 11 of the overflow channel 7 have narrowing ends

20 12 and 13 with a snap lock 14. Around the working channel 2, there are the outer ribs 21 on the extended mount 22. Two of these ribs, which are placed symmetrically, make up the lateral closing brackets 23 with their upper wavy surfaces 24. In the ring-shaped recess 4, there is a

25 packing 25, the lower surface 26 of which is placed on the microscopic slide 6. The monolithic lower part 15 has a circular axial outer recess 16 with a border 17 and a rectangular recess 27 with the border 28 with the edges that are parallel with the edges of the lateral walls 18,

30 on which the lower surface of the microscopic slide 6 is located. The outer lateral surfaces 18 are connected by elastic elements 19 with spring detents 20. According to the invention, the device operates as follows:

35 The pouring device 29, which closes the overflow channel 7, is put on the extended mounted device, which has a microscopic slide 6 between the monolithic upper part 1, which is connected with the monolithic lower part 15 by the spring detents 20 on the upper wavy surfaces 24 0 of the closing brackets 23. After that, the structural biological fluid of a low cellular structure flows to the working channel 2 and later, the narrowing end 12 is closed with a snap lock 14. Next, the device is located in the centrifugal separator, so that the centrifugal 5 forces act along the symmetrical axes 8 of the working channel 2. After the structural fluid has undergone the action of the centrifugal separation, the centrifugal separator is stopped and the supernatant autonomously flows through the overflow channel 7 to the pouring device 0 29, which is taken off from the device and corked. This helps to preserve the entire fluid volume of the tested structural fluid. Next, after the spring detents are taken off, the monolithic upper part 1 is removed; the microscopic slide 6 with the sediment is withdrawn for further research.

After the biological fluid has been separated by a centrifugal separator, and when the research does not require the preservation of the fluid part of the tested biological fluid, it is withdrawn from the overflow channel 7 through the opening, which is located at its end 12 and has a snap lock 14.

Claims

Patent claims

1. The device for obtaining the sediment and the supernatant from structural biological fluids of a low cellular structure under the effect of the centrifugal forces, that are chiefly developed under the action of the centrifugal separation, has the frame with the entrance channel with a longitudinal axis, located at the horizontal plane of the action of the centrifugal forces. Over the microscopic slide, there is a sedimentation zone. The device i s c h a r a c t e r i z e d by a monolithic upper part /!/ with the axial working channel /2/ connected in the lower part /3/ over the ring-shaped recess /4/ close to the upper surface /5/ of the microscopic slide /6/ with the overflow channel /!/, the symmetrical axes of which /8 and 9/ make up the angleo = 15 -- 30 ; while .the outer surface /10/ of the working channel /2/ and the outer surface /ll/ of the overflow channel /!/ have narrowing ends /12 and 13/ with snap locks /14/ and the monolithic lower part /15/ with the circular axial outer recess /16/, the borders /17/ of which make up the basis for the lower surface of the microscopic slide /6/, while the outer lateral surfaces /18/ are favorably connected by the elastic elements /19/ with detents /20/.

2. According to the patent claims 1, the device i s c h a r a c t e r i z e d by having the outer ribs /21/ and the extended mount /22 and 17/ around the working channel /2/, while the two ribs that are located symmetrically /21/ make up lateral closing brackets /23/ with the upper wavy surfaces /24/.

3. According to the patent claims 1, the device i s c h a r a c t e r i z e d by having the packing /25/ in the ring-shaped recess /4/, the lower surface /26/ of which rests on the microscopic slide /6/.

4. According to the patent claims 1, the device i s c h a r a c t e r i z e d by having the rectangular recess /27/ in the monolithic lower part /15/, the borders /28/ of which are parallel with the edges of the lateral walls /18/, make up the basis for the lower surface of the microscopic slide /6/.