WO1995002456A1 - Procede et instrument de dispersion d'agregats concasses - Google Patents

Procede et instrument de dispersion d'agregats concasses Download PDF

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Publication number
WO1995002456A1
WO1995002456A1 PCT/JP1994/000603 JP9400603W WO9502456A1 WO 1995002456 A1 WO1995002456 A1 WO 1995002456A1 JP 9400603 W JP9400603 W JP 9400603W WO 9502456 A1 WO9502456 A1 WO 9502456A1
Authority
WO
WIPO (PCT)
Prior art keywords
bag
sample
flat plate
crushing
dispersing
Prior art date
Application number
PCT/JP1994/000603
Other languages
English (en)
Japanese (ja)
Inventor
Masahiro Takemoto
Hiroshige Yamauchi
Original Assignee
Shimakyu Chemical Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimakyu Chemical Co., Ltd. filed Critical Shimakyu Chemical Co., Ltd.
Publication of WO1995002456A1 publication Critical patent/WO1995002456A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/55—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being contained in a flexible bag submitted to periodical deformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising

Definitions

  • the present invention relates to a crushing and dispersing method for mechanically crushing and dispersing various kinds of samples such as food soil and the equipment used therefor. . Background technology
  • the present invention is intended to facilitate and efficiently disintegrate and disperse various kinds of samples; / "o
  • the crushing and dispersing device which has been made to achieve the above object, is a bag made of synthetic resin containing a sample and a dispersing medium.
  • a solid flat plate having a mounting surface on which the mounting surface can be mounted, and having a protruding portion provided on the mounting surface at a predetermined interval; It is characterized in that it has a roller for rubbing the sample stored in the above-mentioned bag placed on a flat plate and rubbing it.
  • the concave portion between the plurality of protrusions provided on the mounting surface of the flat plate is provided on the surface of the roller. It is preferred that the protrusions that can be fitted to the are provided at a predetermined interval.
  • the flat plate of the crushing and dispersing device described above is used.
  • a sealing means for sealing the opening portion of the bag body and a securing means for fixing the bag body on the flat plate is provided.
  • the method of crushing and dispersing according to the present invention is as follows. A sample and a dispersing medium are stored in a synthetic resin bag, the opening of the bag is sealed, and the surface of the bag is sealed in advance. The bags are laid one on top of the other on a solid flat plate with protruding parts at a predetermined interval, and the bags are pressed and massaged. It is unique.
  • the sample and the dispersion medium are stored in a bag formed of a soft synthetic resin or the like, and the opening of the bag is opened. Seal the part. Subsequently, the bag body is laid and fixed on a solid flat plate having a projection on the surface. Here, the bag is pressed with a roller or the like, and the bag is rubbed and handled. At this time, the sample in the bag is sandwiched between the protruding part of the flat plate and the lip, and when the crushed tag or the roller rubs the bag, The violent water flow generated in Disperse evenly.
  • the sample When the solid sample is stored in a synthetic resin bag together with the dispersion medium and sealed, and compressed and rubbed from the outside of the bag, the sample is It is difficult to crush and disperse in + minutes as it slides in the bag, but according to the present invention, it contains and seals the sample and the dispersion medium.
  • the bag is placed on a flat plate with a large number of protrusions on its surface and pressed and squeezed, so that the bag also protrudes inside the bag. Since the part is formed, the sample can be sufficiently crushed and dispersed without catching and sliding on the protrusion.
  • the groove of the protrusion formed in the bag by the protrusion on the flat plate surface is used.
  • the dispersing medium moves violently, causing complicated changes in water pressure.Thus, if the sample is sufficiently crushed and dispersed, micro-organisms and useful components will not be removed. Will be fully extracted from the sample.
  • the present invention has a simple structure, and can crush and disperse the sample efficiently and efficiently.
  • the present invention uses a synthetic resin bag as a container for storing the sample and the dispersing medium, and prepares a large number of such bags. Discard In this way, a large number of samples can be processed efficiently.
  • FIG. 1 is an overall diagram showing the configuration of the crushing and dispersing device of the embodiment according to the present invention
  • Fig. 2 is the crushing and dispersing device shown in Fig. 1.
  • FIG. 3 is a lateral view of the device
  • FIG. 3 is a plan view showing a flat plate having a large number of protrusions. Best modes for carrying out the invention The following describes an embodiment of the present invention together with a drawing.
  • the crushing and dispersing device 1 is a solid flat plate having a large number of elliptic columnar protrusions 5 on the upper surface.
  • a bag 12 made of synthetic resin for storing the dispersion medium and a sealing member for fixing the opening of the bag 12 and fixing it on the flat plate 11.
  • Sealing 'Fixing device 13 is a well-known fan-shaped structure that fits a long groove and a protrusion, and seals and fixes the bag body by sandwiching it between them. It is a so-called polyclip.
  • the crushing and dispersing device 1 configured as described above contains a sample to be crushed and dispersed and a dispersion medium in a synthetic resin bag 12 and stores the crushing and dispersing medium.
  • the opening of the bag body is sealed by using the sealing device 13 provided on the plate 11 and fixed on the flat plate 11. Then, by squeezing and rubbing the bag body 12 with the mouth-line 14, the sample can be uniformly crushed and dispersed in the dispersion medium. .
  • the size of the synthetic resin bag body 12 constituting the crushing and dispersing equipment is not particularly limited, but the sample 5 to 5 When 10 g of the dispersion medium is 10 times the amount of the sample, a width of 6 to 15 cm and a length of about 10 to 25 cm is easy to handle.
  • the thickness of the synthetic resin film constituting the bag body is not particularly limited, and depends on the material of the film. Although it may be different, it is good if it has a strength that is soft when rubbed and handled with a mouth-lur, etc., and that it does not break further, for example, Pollier In the case of a titanium film, a thickness of about 0.04 to 0.12 mm is desired.
  • the size of the flat plate 11 is not particularly limited, the bag 12 containing the sample and the dispersion medium is not limited to the size. It should be large enough to be completely overlapped and arranged without protruding.
  • the thickness is not particularly limited, but in consideration of the handling convenience and strength, it is hard. In the case of salted pinyl, about 10 to 20 mm is desirable.
  • the size of the protruding portion 15 in the shape of an oval column is not particularly limited, but the major axis is 4 to 2 ⁇ mm and the minor axis is 2 mm. The sample can be sufficiently smashed if it is ⁇ 10 mm and the distance between the protrusions is 2 ⁇ 10 mm.
  • the present invention is not limited to the above embodiment.
  • the bag body 12 is pressed and rubbed with one roller 14, but a large number of small mouth pieces are used.
  • the bag 12 may be pressed and rubbed using a racket, or the finger may be used when the sample is small and the bag 12 is small. You can use pressure and rub and rub.
  • the bag body 12 is pressed and rubbed with the roller 14 having a flat surface, and the mouth is used. To the projections 15 of the flat plate 11 on the surface of By setting up protrusions, the sample can be broken and dispersed more efficiently.
  • a large number of oblong protrusions 15 are formed on the surface of the flat plate 11, but the protrusions are formed.
  • the shape of the raised part is not limited to an oval column, but is not limited to a rectangular column, a triangular column, an oval cone, a square pyramid, or a triangular pyramid. It doesn't matter which shape you choose.
  • the opening of the bag body 12 is sealed by using a sealing device 13 provided at one end of the flat plate 11.
  • the sealing device at the opening may be configured separately from the flat plate 11, for example, by fitting a groove and a protrusion near the opening of the bag body 12.
  • the sealing part of the so-called "political" of the structure to be formed may be integrally formed.
  • the bag body 12 is made of a polystyrene having a thickness of 0.08 mm, a width of 100 mm and a length of 180 mm, and a large number of bags are provided on the upper surface.
  • the plate 11 is made of a hard salted vinyl plate with a length of 220 mm x width of 140 mm and a thickness of 15 mm, and an elliptical protrusion with a width of 50 mm x length of 15 mm.
  • the bag body 12 containing the sample and the dispersion medium was sterilized by irradiating r-rays, and then each sample shown in Table 1 was scissored. Shred and store 5.0 g each in bags 12 with a large number of preparations ⁇ Bags 12 containing samples further kill bacteria After injecting 45.0 ml of saline, place it on top of the flat plate 11 and place it in place.The opening is sealed.'Seal with the fixing device 13 and fix it. did. Next, remove the bag 12 containing the sample and the dispersion medium. Apply 1 to 2 minutes of pressure with a roller 14 or handle it to make a sample milk powder ⁇
  • Comparative Example 1 A sample (10.0 g) was stored in a dry-heat-killed homogenizer cup, and 90 ml of sterilized physiological saline was further injected. After that, the homogenize is performed as a cup-type homogenizer for 1 minute by rotating it at 100,000 times. (Trade name: Nihon Seiki Seisakusho Co., Ltd.)
  • Comparative Example 2 10.0 g of the sample was stored in a polystyrene bag for sterilization after sterilization by r-ray sterilization, and further sterilization and physiological saline 90 After injecting 0 mI, perform homogenization (stomatching) for 1 minute, and use a stomaker as a stomaker. 0 (Product name: GUNZE INDUSTRIAL CO., LTD.)
  • the number of micro-organisms in a sample milk powder homogenized by each method is measured by a conventional method, the extraction efficiency of micro-organisms is examined, and fco micro-organisms are examined. Table 1 shows the measurement results.
  • Comparative Example 1 was used in Example 2 of the present invention.
  • the extraction efficiency of the cup-type homogenizer method and the extraction efficiency almost the same as that of the sampler of Comparative Example 2 were not obtained. It has been proved that crushing and dispersion can be sufficiently performed. Thus, the crushing and scattering instrument 1 of this embodiment requires complicated equipment. It was proved that the sample could be easily crushed, dispersed and milked.
  • crushing and dispersing samples of foods, living organisms, etc. in a very simple and extremely inexpensive manner. Can be obtained.

Abstract

La présente invention se rapporte à la dispersion selon un procédé simple, efficace et excellent de divers échantillons d'agrégats concassés sans mise en ÷uvre d'un appareil coûteux. Un échantillon et un dispositif de dispersion sont placés et enfermés dans un sac (12) de résine synthétique, et ce sac est placé et fixé sur un plateau dur (11) présentant plusieurs saillies (15) sur sa surface extérieure. Le sac est comprimé par un rouleau (14) de manière à être frotté et écrasé; ce faisant, l'échantillon se trouvant dans le sac (12) est pincé par les saillies (15) du plateau (11) et du rouleau (14) et écorché et broyé. L'échantillon est agité par un violent courant d'eau qui survient au moment où le sac (12) est frotté et écrasé par le rouleau (14), de sorte que l'échantillon est uniformément dispersé dans le dispositif de dispersion.
PCT/JP1994/000603 1993-07-14 1994-04-08 Procede et instrument de dispersion d'agregats concasses WO1995002456A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP5196864A JPH0727686A (ja) 1993-07-14 1993-07-14 破砕分散用器具
JP5/196864 1993-07-14

Publications (1)

Publication Number Publication Date
WO1995002456A1 true WO1995002456A1 (fr) 1995-01-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1994/000603 WO1995002456A1 (fr) 1993-07-14 1994-04-08 Procede et instrument de dispersion d'agregats concasses

Country Status (2)

Country Link
JP (1) JPH0727686A (fr)
WO (1) WO1995002456A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1531936A2 (fr) * 2002-07-26 2005-05-25 Applera Corporation Element de commande pour vannes deformables dans un dispositif microfluidique et procede correspondant
WO2005056748A1 (fr) * 2003-12-08 2005-06-23 Covaris, Inc. Appareil et procedes de preparation d'echantillon
US7686500B2 (en) 1998-10-28 2010-03-30 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US7687039B2 (en) 1998-10-28 2010-03-30 Covaris, Inc. Methods and systems for modulating acoustic energy delivery
US7757561B2 (en) 2005-08-01 2010-07-20 Covaris, Inc. Methods and systems for processing samples using acoustic energy
US8435462B2 (en) 2000-06-28 2013-05-07 3M Innovative Properties Company Sample processing devices

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2957670A1 (fr) * 2010-03-18 2011-09-23 Sas Laboratoire Machine a preparer les echantillons et a les reduire en doses
JP5889822B2 (ja) * 2013-03-28 2016-03-22 Ｄｉｃプラスチック株式会社 検体抽出キット、および、検体抽出方法
CN104117401B (zh) * 2014-06-30 2016-04-27 张家港市兰航机械有限公司 辊式散料装置
US10422728B2 (en) 2014-09-26 2019-09-24 Denka Seiken Co. Sample extraction kit and sample extraction method
CN107881100B (zh) * 2017-12-08 2021-04-09 中国环境科学研究院 仿生干式厌氧发酵系统和方法
CN111940040B (zh) * 2020-06-22 2022-06-07 许秋梅 一种熏香制作用柏籽碾碎装置

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Publication number Priority date Publication date Assignee Title
JPS4622297Y1 (fr) * 1968-08-08 1971-08-02
JPS63138017U (fr) * 1987-03-05 1988-09-12

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4622297Y1 (fr) * 1968-08-08 1971-08-02
JPS63138017U (fr) * 1987-03-05 1988-09-12

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MICROFILM OF THE SPECIFICATION AND DRAWINGS ANNEXED TO THE WRITTEN APPLICATION OF JAPANESE UTILITY MODEL, Application No. 135590/1987, (Laid-open No. 041300/1989) (RYOTA KATAHIRA), 13 March 1989, pages 12-13, 14-17. *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7686500B2 (en) 1998-10-28 2010-03-30 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US7687039B2 (en) 1998-10-28 2010-03-30 Covaris, Inc. Methods and systems for modulating acoustic energy delivery
US8435462B2 (en) 2000-06-28 2013-05-07 3M Innovative Properties Company Sample processing devices
EP1531936A2 (fr) * 2002-07-26 2005-05-25 Applera Corporation Element de commande pour vannes deformables dans un dispositif microfluidique et procede correspondant
EP1531936A4 (fr) * 2002-07-26 2005-09-07 Applera Corp Element de commande pour vannes deformables dans un dispositif microfluidique et procede correspondant
US7201881B2 (en) 2002-07-26 2007-04-10 Applera Corporation Actuator for deformable valves in a microfluidic device, and method
WO2005056748A1 (fr) * 2003-12-08 2005-06-23 Covaris, Inc. Appareil et procedes de preparation d'echantillon
US7328628B2 (en) 2003-12-08 2008-02-12 Covaris, Inc. Apparatus and methods for sample preparation
US7677120B2 (en) 2003-12-08 2010-03-16 Covaris, Inc. Apparatus for sample preparation
US7757561B2 (en) 2005-08-01 2010-07-20 Covaris, Inc. Methods and systems for processing samples using acoustic energy

Also Published As

Publication number Publication date
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