WO1994029350A3 - Recombinant disulfide-stabilized polypeptide fragments having binding specificity - Google Patents

Recombinant disulfide-stabilized polypeptide fragments having binding specificity Download PDF

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Publication number
WO1994029350A3
WO1994029350A3 PCT/US1994/006687 US9406687W WO9429350A3 WO 1994029350 A3 WO1994029350 A3 WO 1994029350A3 US 9406687 W US9406687 W US 9406687W WO 9429350 A3 WO9429350 A3 WO 9429350A3
Authority
WO
WIPO (PCT)
Prior art keywords
stabilized
binding specificity
polypeptide fragments
molecules
disulfide
Prior art date
Application number
PCT/US1994/006687
Other languages
French (fr)
Other versions
WO1994029350A2 (en
Inventor
Ira H Pastan
Byungkook Lee
Sun-Hee Jung
Ulrich Brinkmann
Original Assignee
Us Health
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health filed Critical Us Health
Priority to DK94921956T priority Critical patent/DK0703926T3/en
Priority to DE69412614T priority patent/DE69412614T2/en
Priority to JP50217295A priority patent/JP3474574B2/en
Priority to AU72464/94A priority patent/AU682705B2/en
Priority to EP94921956A priority patent/EP0703926B1/en
Priority to CA002164984A priority patent/CA2164984C/en
Publication of WO1994029350A2 publication Critical patent/WO1994029350A2/en
Publication of WO1994029350A3 publication Critical patent/WO1994029350A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • A61K47/6817—Toxins
    • A61K47/6829—Bacterial toxins, e.g. diphteria toxins or Pseudomonas exotoxin A
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/866—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody fragment, e.g. fab', fab, fv, fc, heavy chain or light chain
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/867—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody produced via recombinant dna technology

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The present invention relates to disulfide-stabilized recombinant polypeptide molecules which have the binding ability and specificity for another peptide, such as the variable region of an antibody molecule. Methods of producing these molecules and nucleic acid sequences encoding these molecules are also described. In particular, the invention discloses Fv antibody fragments stabilized by a disulfide bond connecting the VH and VL regions of the Fv fragment. The α and β chains of T cells receptors may be similarly stabilized by means described in the invention.
PCT/US1994/006687 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity WO1994029350A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DK94921956T DK0703926T3 (en) 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments with binding specificity
DE69412614T DE69412614T2 (en) 1993-06-14 1994-06-14 RECOMBINANT DISULFID-STABILIZED POLYPEPTIDES WITH BINDING SPECIFICITY
JP50217295A JP3474574B2 (en) 1993-06-14 1994-06-14 Recombinant disulfide stabilized polypeptide fragments with binding specificity
AU72464/94A AU682705B2 (en) 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity
EP94921956A EP0703926B1 (en) 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity
CA002164984A CA2164984C (en) 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/077,252 US5747654A (en) 1993-06-14 1993-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity
US08/077,252 1993-06-14

Publications (2)

Publication Number Publication Date
WO1994029350A2 WO1994029350A2 (en) 1994-12-22
WO1994029350A3 true WO1994029350A3 (en) 1995-02-02

Family

ID=22136984

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/006687 WO1994029350A2 (en) 1993-06-14 1994-06-14 Recombinant disulfide-stabilized polypeptide fragments having binding specificity

Country Status (10)

Country Link
US (3) US5747654A (en)
EP (1) EP0703926B1 (en)
JP (1) JP3474574B2 (en)
AT (1) ATE169932T1 (en)
AU (1) AU682705B2 (en)
CA (1) CA2164984C (en)
DE (1) DE69412614T2 (en)
DK (1) DK0703926T3 (en)
ES (1) ES2124419T3 (en)
WO (1) WO1994029350A2 (en)

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US9382323B2 (en) 2009-04-02 2016-07-05 Roche Glycart Ag Multispecific antibodies comprising full length antibodies and single chain fab fragments
US11845783B2 (en) 2010-09-21 2023-12-19 Altor BioScience, LLC. Multimeric IL-15 soluble fusion molecules and methods of making and using same
US11992516B2 (en) 2014-06-30 2024-05-28 Altor BioScience, LLC. Compositions comprising IL-15-based molecules and immune checkpoint inhibitor antibodies
US11993642B2 (en) 2009-04-07 2024-05-28 Hoffmann-La Roche Inc. Trivalent, bispecific antibodies

Families Citing this family (145)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4425115A1 (en) * 1994-07-15 1996-01-18 Boehringer Mannheim Gmbh Method for modifying the stability of antibodies
DE69629580D1 (en) * 1995-10-13 2003-09-25 Us Gov Health & Human Serv IMMUNOTOXIN CONTAINING A DISULFIDE-STABILIZED ANTIBODY FRAGMENT
US5986065A (en) 1997-03-10 1999-11-16 Sunol Molecular Corporation Antibodies for inhibiting blood coagulation and methods of use thereof
ES2300122T3 (en) 1997-03-20 2008-06-01 The Government Of The Usa As Represented By The Secretary Of Department Of Health And Human Services RECOMBINANT AND IMMUNOCATED PLAYING ANTIBODIES AGAINST CD-22 CARRIER CELLS AND TUMORS.
DE19721700C1 (en) * 1997-05-23 1998-11-19 Deutsches Krebsforsch Mutant OKT3 antibody
US6809184B1 (en) * 1997-12-01 2004-10-26 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Antibodies, including FV molecules, and immunoconjugates having high binding affinity for mesothelin and methods for their use
US6519003B1 (en) 1998-03-26 2003-02-11 Eastman Kodak Company Camera with combination four-way directional and mode control interface
DE50013935D1 (en) * 1999-04-13 2007-02-15 Wilex Ag DIAGNOSTIC AND THERAPEUTIC USE OF ANTIBODIES TO THE UROKINASE RECEPTOR
DK1180123T3 (en) 1999-05-27 2008-10-13 Us Gov Health & Human Serv Immune conjugates that have high binding affinity
WO2000075162A1 (en) * 1999-06-07 2000-12-14 Mirus Corporation A compound containing a labile disulfide bond
EP1074563A1 (en) * 1999-08-02 2001-02-07 F. Hoffmann-La Roche Ag Chimeric polypeptides enhancing dimer formation through electrostatic interactions and disulfide bond, method for production and uses thereof
US7118745B1 (en) 1999-09-27 2006-10-10 The Regents Of The University Of California Engineering antibodies that bind irreversibly
JP2003516121A (en) * 1999-09-27 2003-05-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Engineering antibodies that bind irreversibly
ES2367475T3 (en) * 1999-10-15 2011-11-03 Avatar Medical, L.L.C. STABILIZED PROTEINS.
US6492498B1 (en) 1999-11-15 2002-12-10 Regents Of The University Of Minnesota Multimeric immunotoxins
CN101712721A (en) 2000-06-05 2010-05-26 阿尔托生物科学有限公司 T cell receptor fusions and conjugates and methods of use thereof
US7829084B2 (en) 2001-01-17 2010-11-09 Trubion Pharmaceuticals, Inc. Binding constructs and methods for use thereof
US7754208B2 (en) * 2001-01-17 2010-07-13 Trubion Pharmaceuticals, Inc. Binding domain-immunoglobulin fusion proteins
US20030133939A1 (en) * 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
WO2002073193A1 (en) * 2001-03-12 2002-09-19 Board Of Regents, The University Of Texas System Computer-based strategy for peptide and protein conformational ensemble enumeration and ligand affinity analysis
US20030118585A1 (en) * 2001-10-17 2003-06-26 Agy Therapeutics Use of protein biomolecular targets in the treatment and visualization of brain tumors
JP4361786B2 (en) * 2001-06-14 2009-11-11 ザ スクリプス リサーチ インスティチュート Stabilized proteins with engineered disulfide bonds
PL208712B1 (en) * 2001-08-31 2011-05-31 Avidex Ltd Soluble t cell receptor
AU2003209886A1 (en) * 2002-03-14 2003-09-22 Qlt Inc. Cancer associated araf1 protein kinase and its uses
EP1561759B9 (en) * 2002-10-11 2009-08-26 Chugai Seiyaku Kabushiki Kaisha Cell death-inducing agent
US20040101929A1 (en) * 2002-11-22 2004-05-27 Chi-Yao Chang Expressing mammalian protein complexes in fish
US7820787B2 (en) * 2003-01-23 2010-10-26 The Regents Of The University Of California Multi-functional antibodies
GB0304068D0 (en) * 2003-02-22 2003-03-26 Avidex Ltd Substances
US20050054572A1 (en) * 2003-07-03 2005-03-10 Marshall Christopher P. Methods for obtaining molecules with reduced immunogenicity
US7754209B2 (en) * 2003-07-26 2010-07-13 Trubion Pharmaceuticals Binding constructs and methods for use thereof
AU2004293471C1 (en) 2003-11-25 2011-02-24 The Government Of The United States, As Represented By The Secretary Of Health And Human Services Mutated anti-CD22 antibodies and immunoconjugates
GB0328363D0 (en) 2003-12-06 2004-01-14 Imp College Innovations Ltd Therapeutically useful molecules
JP4767016B2 (en) * 2003-12-12 2011-09-07 中外製薬株式会社 Cell death inducer
WO2005056602A1 (en) * 2003-12-12 2005-06-23 Chugai Seiyaku Kabushiki Kaisha Method of screening modified antibody having agonistic activity
US20080206229A1 (en) * 2003-12-12 2008-08-28 Koichiro Ono Modified Antibodies Recognizing Receptor Trimers or Higher Multimers
WO2005089409A2 (en) * 2004-03-17 2005-09-29 University Of Hawaii Sensor constructs and detection methods
WO2005100560A1 (en) * 2004-04-09 2005-10-27 Chugai Seiyaku Kabushiki Kaisha Cell death inducer
JP5563194B2 (en) * 2004-06-29 2014-07-30 イムノコア リミテッド Cells expressing modified T cell receptors
JP5057967B2 (en) * 2005-03-31 2012-10-24 中外製薬株式会社 sc (Fv) 2 structural isomer
EP1927367A4 (en) * 2005-05-18 2009-08-12 Univ Tokushima Novel pharmaceutical using anti-hla antibody
KR101367544B1 (en) * 2005-06-10 2014-02-26 추가이 세이야쿠 가부시키가이샤 Stabilizer for protein preparation comprising meglumine and use thereof
JP5085322B2 (en) * 2005-06-10 2012-11-28 中外製薬株式会社 Pharmaceutical composition containing sc (Fv) 2
RS54088B1 (en) 2005-07-25 2015-10-30 Emergent Products Development Seattle Llc B-cell reduction using cd37-specific and cd20-specific binding molecules
ES2660026T3 (en) 2005-07-29 2018-03-20 The Government Of The United States Of America, As Represented By The Secretary Of Health And Human Services Exotoxins of mutated pseudomonas with reduced antigenicity
JP5374359B2 (en) 2006-03-17 2013-12-25 バイオジェン・アイデック・エムエイ・インコーポレイテッド Stabilized polypeptide compounds
KR101571027B1 (en) 2006-06-12 2015-11-23 이머전트 프로덕트 디벨롭먼트 시애틀, 엘엘씨 Single-chain multivalent binding proteins with effector function
CA2657385A1 (en) * 2006-07-13 2008-01-17 Naoki Kimura Cell death inducer
CL2008000719A1 (en) * 2007-03-12 2008-09-05 Univ Tokushima Chugai Seiyaku THERAPEUTIC AGENT FOR CANCER RESISTANT TO CHEMOTHERAPEUTIC AGENTS THAT UNDERSTAND AN ANTIBODY THAT RECOGNIZES IT CLASS I AS ACTIVE INGREDIENT; PHARMACEUTICAL COMPOSITION THAT INCLUDES SUCH ANTIBODY; AND METHOD TO TREAT CANCER RESISTANT TO
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CA2698357C (en) 2007-09-04 2017-06-06 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Deletions in domain ii of pseudomonas exotoxin a that reduce non-specific toxicity
US9266967B2 (en) 2007-12-21 2016-02-23 Hoffmann-La Roche, Inc. Bivalent, bispecific antibodies
US20090162359A1 (en) 2007-12-21 2009-06-25 Christian Klein Bivalent, bispecific antibodies
NZ588671A (en) * 2008-04-11 2012-11-30 Emergent Product Dev Seattle Cd37 immunotherapeutic and combination with bifunctional chemotherapeutic thereof
US8268314B2 (en) 2008-10-08 2012-09-18 Hoffmann-La Roche Inc. Bispecific anti-VEGF/anti-ANG-2 antibodies
WO2010112194A1 (en) 2009-04-02 2010-10-07 F. Hoffmann-La Roche Ag Antigen-binding polypeptides and multispecific antibodies comprising them
EP2417164A1 (en) 2009-04-07 2012-02-15 Roche Glycart AG Bispecific anti-erbb-2/anti-c-met antibodies
CN102378768A (en) 2009-04-07 2012-03-14 罗氏格黎卡特股份公司 Bispecific anti-erbb-3/anti-c-met antibodies
KR101431319B1 (en) 2009-05-27 2014-08-20 에프. 호프만-라 로슈 아게 Tri- or tetraspecific antibodies
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
US9050375B2 (en) 2009-07-06 2015-06-09 Hoffmann-La Roche, Inc. Bi-specific digoxigenin binding antibodies
WO2011031441A1 (en) 2009-08-28 2011-03-17 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Therapy with a chimeric molecule and a pro-apoptotic agent
WO2011030107A1 (en) 2009-09-10 2011-03-17 Ucb Pharma S.A. Multivalent antibodies
ES2544805T3 (en) 2009-09-11 2015-09-04 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pseudomonas Exotoxin A enhanced with reduced immunogenicity
SI2477648T1 (en) 2009-09-15 2022-09-30 The Board Of Trustees Of The Leland Stanford Junior University Synergistic anti-cd47 therapy for hematologic cancers
AU2010296018B2 (en) 2009-09-16 2016-05-05 Genentech, Inc. Coiled coil and/or tether containing protein complexes and uses thereof
GB201005063D0 (en) * 2010-03-25 2010-05-12 Ucb Pharma Sa Biological products
GB0920127D0 (en) * 2009-11-17 2009-12-30 Ucb Pharma Sa Antibodies
WO2011100455A1 (en) 2010-02-12 2011-08-18 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inhibition of antibody responses to foreign proteins
GB201005064D0 (en) * 2010-03-25 2010-05-12 Ucb Pharma Sa Biological products
AR080794A1 (en) 2010-03-26 2012-05-09 Hoffmann La Roche BIVING SPECIFIC ANTIBODIES ANTI-VEGF / ANTI-ANG-2
AR080793A1 (en) 2010-03-26 2012-05-09 Roche Glycart Ag BISPECIFIC ANTIBODIES
MX340558B (en) 2010-08-24 2016-07-14 F Hoffmann-La Roche Ag * Bispecific antibodies comprising a disulfide stabilized - fv fragment.
WO2012025525A1 (en) 2010-08-24 2012-03-01 Roche Glycart Ag Activatable bispecific antibodies
US11053299B2 (en) 2010-09-21 2021-07-06 Immunity Bio, Inc. Superkine
US9505826B2 (en) 2010-12-22 2016-11-29 Teva Pharmaceuticals Australia Pty Ltd Modified antibody with improved half-life
EP2655413B1 (en) 2010-12-23 2019-01-16 F.Hoffmann-La Roche Ag Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
BR112013020338A2 (en) 2011-02-28 2016-10-18 Hoffmann La Roche monovalent antigen binding protein, pharmaceutical composition, use of monovalent antigen binding protein, method for treating a patient in need of therapy, method for preparing a monovalent antigen binding protein, nucleic acid, vector and cell hostess
WO2012116926A1 (en) 2011-02-28 2012-09-07 F. Hoffmann-La Roche Ag Antigen binding proteins
JP2014510084A (en) 2011-03-17 2014-04-24 ラモット・アット・テル・アビブ・ユニバーシテイ・リミテッド Bispecific and monospecific asymmetric antibodies and methods for their production
EP2502934B1 (en) 2011-03-24 2018-01-17 Universitätsmedizin der Johannes Gutenberg-Universität Mainz Single chain antigen recognizing constructs (scARCs) stabilized by the introduction of novel disulfide bonds
CN103648525B (en) 2011-05-06 2016-08-24 由卫生与公共服务部部长代表的美国政府 The recombinant immunotoxin of targeting mesothelin
BR112013031262A2 (en) 2011-06-09 2016-11-22 Us Health "exotoxins a from pseudomonas with less immunogenic bet cell epitopes, and their use in cancer treatment and prevention, as well as chimeric molecule, nucleic acid, expression vector, cells, pharmaceutical composition and methods to inhibit target cell growth and endotoxin production and pseudomonas and chimeric molecule ".
US9206240B2 (en) 2011-09-16 2015-12-08 The United States of America, as represented by the Secretary, Department of Helath and Human Services Pseudomonas exotoxin A with less immunogenic B cell epitopes
BR112014019579A2 (en) 2012-02-10 2019-10-15 Genentech, Inc SINGLE CHAIN ANTIBODY, POLYNUCLEOTIDE, VECTOR, HOST CELL, METHOD OF PRODUCTION OF A SINGLE CHAIN ANTIBODY, HETEROMULTYMER AND METHOD OF PRODUCTION
EP2867254B1 (en) 2012-06-27 2017-10-25 F. Hoffmann-La Roche AG Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof
WO2014001324A1 (en) 2012-06-27 2014-01-03 Hoffmann-La Roche Ag Method for selection and production of tailor-made highly selective and multi-specific targeting entities containing at least two different binding entities and uses thereof
AU2013288641B2 (en) 2012-07-13 2017-07-06 Roche Glycart Ag Bispecific anti-VEGF/anti-ANG-2 antibodies and their use in the treatment of ocular vascular diseases
US9671405B2 (en) 2012-09-19 2017-06-06 Cornell University Identifying taxane sensitivity in prostate cancer patients
WO2014069647A1 (en) * 2012-11-05 2014-05-08 全薬工業株式会社 Antibody and antibody composition production method
WO2014108854A1 (en) 2013-01-09 2014-07-17 Fusimab Ltd. Monospecific anti-hgf and anti-ang2 antibodies and bispecific anti-hgf/anti-ang2 antibodies
WO2014177459A2 (en) 2013-04-29 2014-11-06 F. Hoffmann-La Roche Ag Fc-receptor binding modified asymmetric antibodies and methods of use
JP2016528167A (en) 2013-04-29 2016-09-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Human FcRn binding modified antibody and method of use
US9890369B2 (en) 2013-06-20 2018-02-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cytolethal distending toxin subunit B conjugated or fused to Bacillus anthracis toxin lethal factor
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BR112016006929A2 (en) 2013-10-11 2017-09-19 Hoffmann La Roche ANTIBODY, NUCLEIC ACID, EXPRESSION VECTOR, HOST CELL, METHODS OF PREPARING ANTIBODY, TREATMENT OF PATIENTS AND GENERATION OF AN ANTIBODY, PHARMACEUTICAL COMPOSITION AND USE OF THE ANTIBODY
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WO2015073878A1 (en) 2013-11-15 2015-05-21 Ur Diet, Llc Real-time satiety biofeedback
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US9738702B2 (en) 2014-03-14 2017-08-22 Janssen Biotech, Inc. Antibodies with improved half-life in ferrets
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US10654908B2 (en) 2014-04-15 2020-05-19 University Of Virginia Patent Foundation Isolated T cell receptors and methods of use therefor
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LT3215528T (en) 2014-11-06 2019-10-25 Hoffmann La Roche Fc-region variants with modified fcrn-binding and methods of use
WO2016087416A1 (en) 2014-12-03 2016-06-09 F. Hoffmann-La Roche Ag Multispecific antibodies
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US20190048055A1 (en) 2017-03-31 2019-02-14 Altor Bioscience Corporation Alt-803 in combination with anti-cd38 antibody for cancer therapies
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US11421029B2 (en) 2017-09-01 2022-08-23 Sichuan Kelun-Biotech Biopharmaceutical Co., Ltd. Recombinant bispecific antibodies to PD-L1 and CTLA-4
US11168138B2 (en) 2018-03-26 2021-11-09 Altor Bioscience, Llc Anti-PDL1, IL-15 and TGF-beta receptor combination molecules
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WO2019234580A1 (en) * 2018-06-04 2019-12-12 Diatheva S.R.L. Anti-cd99 diabody or igg antibody and uses thereof
WO2020018715A1 (en) 2018-07-17 2020-01-23 Massachusetts Institute Of Technology Soluble multimeric immunoglobulin-scaffold based fusion proteins and uses thereof
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US20220306989A1 (en) 2019-08-19 2022-09-29 Universität Basel Cell therapy methods
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CA3224180A1 (en) 2021-07-28 2023-02-02 F. Hoffmann-La Roche Ag Methods and compositions for treating cancer
CN114181310B (en) 2022-02-14 2022-07-05 中山康方生物医药有限公司 anti-TIGIT antibody, and pharmaceutical composition and use thereof
CA3208473A1 (en) 2022-06-22 2023-12-22 Akeso Biopharma, Inc. Pharmaceutical composition and use thereof
WO2024077118A2 (en) 2022-10-06 2024-04-11 Bicara Therapeutics Inc. Multispecific proteins and related methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338745A1 (en) * 1988-04-16 1989-10-25 Celltech Limited Method for producing recombinant DNA proteins
WO1993007286A1 (en) * 1991-09-30 1993-04-15 The United States Of America, Represented By The Secretary, Department Of Health And Human Services Recombinant immunotoxins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946778A (en) * 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5091513A (en) * 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5132405A (en) * 1987-05-21 1992-07-21 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
WO1993006217A1 (en) * 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338745A1 (en) * 1988-04-16 1989-10-25 Celltech Limited Method for producing recombinant DNA proteins
WO1993007286A1 (en) * 1991-09-30 1993-04-15 The United States Of America, Represented By The Secretary, Department Of Health And Human Services Recombinant immunotoxins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A.J.CUMBER ET AL.: "COMPARATIVE STABILITIES IN VITRO AND IN VIVO OF A RECOMBINANT MOUSE ANTIBODY FvCys FRAGMENT AND A bisFvCys CONJUGATE", J.IMMUNOL., vol. 149, no. 1, 1992, pages 120 - 126 *
P.PACK ET AL.: "MINIANTIBODIES: USE OF AMPHIPHATIC HELICES TO PRODUCE FUNCTIONAL, FLEXIBLY LINKED DIMERIC Fv FRAGMENTS WITH HIGH ACTIVITY IN E.COLI", BIOCHEMISTRY, vol. 31, no. 6, 1992, pages 1579 - 84 *
R.GLOCKSHUBER ET AL.: "A COMARISION OF STRATEGIES TO STABILIZE IMMUNOGLOBULIN Fv- FRAGMENTS", BIOCHEMISTRY, vol. 29, no. 6, 1990, pages 1362 - 67 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9382323B2 (en) 2009-04-02 2016-07-05 Roche Glycart Ag Multispecific antibodies comprising full length antibodies and single chain fab fragments
US11993642B2 (en) 2009-04-07 2024-05-28 Hoffmann-La Roche Inc. Trivalent, bispecific antibodies
US11845783B2 (en) 2010-09-21 2023-12-19 Altor BioScience, LLC. Multimeric IL-15 soluble fusion molecules and methods of making and using same
US11992516B2 (en) 2014-06-30 2024-05-28 Altor BioScience, LLC. Compositions comprising IL-15-based molecules and immune checkpoint inhibitor antibodies

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