WO1994025159A1 - Dispositif pour examens sanguins - Google Patents

Dispositif pour examens sanguins Download PDF

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Publication number
WO1994025159A1
WO1994025159A1 PCT/CH1994/000079 CH9400079W WO9425159A1 WO 1994025159 A1 WO1994025159 A1 WO 1994025159A1 CH 9400079 W CH9400079 W CH 9400079W WO 9425159 A1 WO9425159 A1 WO 9425159A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
opening
partial
test vessels
test
Prior art date
Application number
PCT/CH1994/000079
Other languages
German (de)
English (en)
Inventor
Johann Leinhardt
Original Assignee
Diag Human Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diag Human Ag filed Critical Diag Human Ag
Priority to AU65011/94A priority Critical patent/AU6501194A/en
Publication of WO1994025159A1 publication Critical patent/WO1994025159A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates

Definitions

  • the invention relates to a device for blood analysis according to the preamble of claim 1.
  • the object of the invention is to further improve a device of the type mentioned.
  • the object is achieved according to the invention by the characterizing features of claim 1.
  • the novel device can be placed on a simple centrifuge without special aids, which on the one hand simplifies the centrifuge design and on the other hand each device can be centrifuged individually immediately without several such devices must be placed in a complicated mounting system of a centrifuge.
  • Advantageous embodiments of the device are described in claims 2 to 9.
  • the device is designed according to claim 2, so that the sample to be examined can be introduced for all test vessels by means of a one-time filling process, which considerably simplifies handling compared to the known devices in which the sample to be examined for each test vessel must be added separately.
  • connection opening between the first chamber and the second chamber can in principle be at any location, but an embodiment according to claim 3 is preferred. This ensures that the sample to be examined does not immediately enter the first chamber from the second chamber. Only after a certain number of revolutions has been reached does the sample move on the inclined chamber wall into the connection opening and thus into the first chamber.
  • An embodiment of the device according to claim 4 is particularly advantageous, in which case two different substances can then be introduced into the second chamber, for example an erythrocyte into the first partial chamber and a serum to be examined into the second partial chamber. With a gentle rotation, the substance then migrates from the second sub-chamber into the first sub-chamber and can react there. The reaction mixture then migrates from the first sub-chamber into the first chamber at a higher speed. With the help of the new device, numerous pipetting steps can be saved, so that investigations can be carried out more efficiently. It can be expedient if the second partial chamber is an annular chamber common to all test vessels.
  • Claim 6 describes an advantageous embodiment of the device, which can be further improved by the development according to claim 7. This improves the system when filling the device. Particularly advantageous is a further development according to claim 8, which prevents unintentional penetration of substances into the first chamber when filling the second chamber.
  • an embodiment of the device according to claim 9 is advantageous.
  • Figure 1 shows a first device with a lid with six test vessels distributed around the circumference, in plan view
  • FIG. 1 shows the device of Figure 1 in
  • FIG. 3 shows another device with 16 am
  • FIGS. 1 and 2 show a device which is suitable, for example, for determining blood groups.
  • the device contains a carrier disk 2 which can be rotated about its axis 4.
  • the carrier disc has a hub 6 with a bore 8 coaxial with the axis 4, by means of which it can be placed, for example, on a pivot pin of a centrifuge.
  • the carrier disk 2 contains six test vessels 10 ⁇ to 10g, which are arranged on the carrier disk 2 lying along a radial beam 11 to the axis 4.
  • Each test vessel has a first chamber 12 with an external tapered end section 14 and is filled with a known density gradient suitable for the respective examination purpose.
  • a second chamber 16 Connected radially inward to the first chamber 12 is a second chamber 16, which communicates with the first chamber 12 via a connecting opening 18 and has an upper filling opening 20, so that the samples can be introduced horizontally.
  • the chamber wall 22 arranged between the first chamber 12 and the second chamber 16 is inclined on the side 24 facing the second chamber 16 at an angle ⁇ X radially outwards against the connecting opening 18, so that with increasing centrifugal force the content of the second chamber can reach the first chamber 12 via the connection opening 18.
  • the second chamber can now be in one piece or, as in the example shown, can be divided into a first partial chamber 16a and a second partial chamber 16b with corresponding filling openings 20a, 20b by means of a partition wall 26, the first partial chamber 16a of the first chamber 12 is facing.
  • the first partial chamber 16a and the second partial chamber 16b are connected to one another via an overflow 28 which is formed by the upper edge of the partition wall 26.
  • the partition wall 26 is on the side 30 facing the second partial chamber 16b inclined radially outwards at an angle ⁇ , the angle ⁇ being smaller than the angle ot of the chamber wall 22, so that during centrifugation the contents of the second partial chamber 16b first flow into the first partial chamber 16a before penetration of the chamber contents via the Connection opening 18 in the first chamber 12 is possible.
  • the second subchamber 16b can be provided with a serum to be examined and the first subchamber 16a with an erythrocyte with which the serum reacts before it is analyzed in a known manner in the first chamber 12, ie in the density gradient.
  • the second sub-chamber 16b is preferably common to all test vessels, i.e. designed as an annular chamber.
  • the first sub-chamber 16a can be individual for each test vessel or can also be designed as an annular chamber common to all test vessels.
  • the device preferably has a lid 32 which is transparent for observing the test vessels 10 ⁇ to 10 g.
  • the cover 32 can, for example, have an opening 34 which leaves the region of the second sub-chamber 16b, which opening is therefore assigned to the filling opening 20b.
  • the cover 32 can be designed as a fixed cover, wherein the filling opening 20a of each first sub-chamber 16a can be assigned an opening.
  • the lid 32 is preferably designed to be rotatable and has an opening 36 which can be assigned to each filling opening of the first subchambers 16a, the opening 36 moreover from an active position interacting with the test vessels, that is to say the associated filling opening, into an adjacent inactive position Position can be pivoted.
  • the lid can also have a downward-facing cover lug 38 which is arranged in the region of the opening 36 and which covers the connection opening 18 from the second chamber 16 to the first chamber 12 of a test vessel 10 if the opening 36 is assigned to the corresponding test vessel. Only after the lid has been turned further does the cover tab 38 release the connection opening when the opening no longer communicates with the corresponding test vessel.
  • FIG. 3 shows a further device with sixteen radially arranged test vessels 10 to 10 ⁇ , in which the second partial chamber 16b forms an annular chamber common to all test vessels analogous to that of FIG.
  • the first sub-chamber 16a likewise forms a continuous annular chamber, but the bottom and the walls are designed prismatically in such a way that the substance added to the individual test vessels cannot get into the adjacent test vessel.
  • the device shown in FIG. 3 is particularly suitable for antibody identification and for an antibody search test.
  • the inner second partial chamber 16b serves to receive the serum necessary for the examination.
  • the outer partial chamber 16a which is closer to the first chamber 12 and thus to the density gradient, serves to receive erythrocytes.
  • the first sub-chamber 16a simultaneously forms a reaction chamber.
  • the serum to be examined can be introduced into the internal second partial chamber 16b with a single pipetting step.
  • the serum is automatically distributed into the first subchambers 16a by gentle centrifugation, that is to say at low speed, in which the erythrocytes are placed. Only in a second phase of centrifuging at high speed does the reaction mixture pass from the first sub-chamber 16a via the connection opening 18 into the first chamber 12 and thus into the density gradient for the known evaluation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Centrifugal Separators (AREA)

Abstract

Le dispositif comprend un support circulaire (2) disposé horizontalement et tournant autour de son axe (4), sur lequel sont disposées horizontalement des éprouvettes (101, 104) le long d'une ligne radiale. Une première chambre (12) qui présente un gradient de densité est disposée radialement vers l'extérieur. A cette chambre est reliée, par un orifice de liaison (18), une deuxième chambre (16a, 16b) disposée vers l'intérieur. Cette dernière est de préférence subdivisée en une première chambre partielle (16a) et une deuxième chambre partielle (16b) reliées entre elles par un déversoir (28). Le dispositif peut très facilement recevoir des substances à examiner et il peut très facilement être centrifugé.
PCT/CH1994/000079 1993-05-03 1994-04-27 Dispositif pour examens sanguins WO1994025159A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU65011/94A AU6501194A (en) 1993-05-03 1994-04-27 Blood testing device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH134293A CH685312A5 (de) 1993-05-03 1993-05-03 Vorrichtung zur Blutuntersuchung.
CH1342/93-6 1993-05-03

Publications (1)

Publication Number Publication Date
WO1994025159A1 true WO1994025159A1 (fr) 1994-11-10

Family

ID=4208241

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CH1994/000079 WO1994025159A1 (fr) 1993-05-03 1994-04-27 Dispositif pour examens sanguins

Country Status (3)

Country Link
AU (1) AU6501194A (fr)
CH (1) CH685312A5 (fr)
WO (1) WO1994025159A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2773218A1 (fr) * 1997-12-31 1999-07-02 Stago International Dispositif, procede et appareil de mise en oeuvre du procede, pour effectuer un dosage d'au moins un composant particulier dans un echantillon de produit
US11471881B2 (en) 2013-02-07 2022-10-18 Laboratory Corporation Of America Holdings Automated sample processing, fluid distribution, and sedimentation assay
US11714034B2 (en) 2012-11-07 2023-08-01 Laboratory Corporation Of America Holdings Methods and devices for processing samples and counting cells

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2067639A5 (fr) * 1969-11-12 1971-08-20 Guigan Jean
FR2089490A5 (fr) * 1970-04-13 1972-01-07 Union Carbide Corp
EP0106536A2 (fr) * 1982-09-15 1984-04-25 Ortho Diagnostic Systems Inc. Dispositif centrifuge de séparation, de lavage et d'examen de microparticules et méthode d'utilisation
EP0198462A2 (fr) * 1985-04-18 1986-10-22 Opopharma A.G. Séparation de matières d'une dispersion liquide par sédimentation
WO1988010427A1 (fr) * 1987-06-23 1988-12-29 Large Scale Biology Corporation Chromatographe centrifuge rapide
EP0405162A2 (fr) * 1989-06-01 1991-01-02 BEHRINGWERKE Aktiengesellschaft Rotor des cuves
EP0447060A2 (fr) * 1990-03-12 1991-09-18 Beckman Instruments, Inc. Séparation optimalisée par centrifugation

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2067639A5 (fr) * 1969-11-12 1971-08-20 Guigan Jean
FR2089490A5 (fr) * 1970-04-13 1972-01-07 Union Carbide Corp
EP0106536A2 (fr) * 1982-09-15 1984-04-25 Ortho Diagnostic Systems Inc. Dispositif centrifuge de séparation, de lavage et d'examen de microparticules et méthode d'utilisation
EP0198462A2 (fr) * 1985-04-18 1986-10-22 Opopharma A.G. Séparation de matières d'une dispersion liquide par sédimentation
WO1988010427A1 (fr) * 1987-06-23 1988-12-29 Large Scale Biology Corporation Chromatographe centrifuge rapide
EP0405162A2 (fr) * 1989-06-01 1991-01-02 BEHRINGWERKE Aktiengesellschaft Rotor des cuves
EP0447060A2 (fr) * 1990-03-12 1991-09-18 Beckman Instruments, Inc. Séparation optimalisée par centrifugation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LANGER: "zur sedimentationsanalyse wässriger Kunstoffsdispersionen mit der Scheibenzentrifuge", COLLOID AND POLYMER SCIENCE, vol. 257, 1979, pages 522 - 532 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2773218A1 (fr) * 1997-12-31 1999-07-02 Stago International Dispositif, procede et appareil de mise en oeuvre du procede, pour effectuer un dosage d'au moins un composant particulier dans un echantillon de produit
WO1999035483A1 (fr) * 1997-12-31 1999-07-15 Stago International Dispositif, procede et appareil de mise en oeuvre du procede, pour effectuer un dosage d'au moins un composant particulier dans un echantillon de produit
AU737445B2 (en) * 1997-12-31 2001-08-16 Stago International Device, process and apparatus for implementing the process, for dosing at least one particular component in a product sample
US11714034B2 (en) 2012-11-07 2023-08-01 Laboratory Corporation Of America Holdings Methods and devices for processing samples and counting cells
US11471881B2 (en) 2013-02-07 2022-10-18 Laboratory Corporation Of America Holdings Automated sample processing, fluid distribution, and sedimentation assay

Also Published As

Publication number Publication date
CH685312A5 (de) 1995-05-31
AU6501194A (en) 1994-11-21

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