WO1994004272A1 - Dispositif d'essai pour fluides - Google Patents

Dispositif d'essai pour fluides Download PDF

Info

Publication number
WO1994004272A1
WO1994004272A1 PCT/GB1993/001797 GB9301797W WO9404272A1 WO 1994004272 A1 WO1994004272 A1 WO 1994004272A1 GB 9301797 W GB9301797 W GB 9301797W WO 9404272 A1 WO9404272 A1 WO 9404272A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
chamber
receptor means
bore
rim
Prior art date
Application number
PCT/GB1993/001797
Other languages
English (en)
Inventor
Peter Alan Neill
Thomas Lydford
Original Assignee
Hypoguard (Uk) Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hypoguard (Uk) Limited filed Critical Hypoguard (Uk) Limited
Priority to AU49686/93A priority Critical patent/AU4968693A/en
Publication of WO1994004272A1 publication Critical patent/WO1994004272A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the present invention relates to a device, notably to a sample collecting device.
  • an integral fluid sample collector and sample assessment device which comprises: a. a fluid receptor means adapted to receive a sample of a fluid from an external source; b. a substantially closed chamber adapted to receive fluid from the receptor means; c. a member providing at least part of one wall of said chamber, said member carrying one or more reagents adapted to respond to one or more components of the fluid sample and adapted to give an indication of that response which can be detected from the exterior of the chamber; which device is characterised in that it also comprises: d.
  • the chamber has means to vent or accommodate air displaced by the fluid entering the chamber.
  • the device is in the form of a machined or moulded metal, glass or plastic unitary construction body member comprising a cup or recess having an exposed open top into which the sample to be tested is placed.
  • the cup or recess is connected by a bore to a chamber within the body which has one face thereof formed at least in part from a demountable generally planar member.
  • This member carries the reagent for the test to be carried out on or accessible from one face thereof and is adapted to provide a visual display of the response from the reagent to the sample at the other face thereof.
  • the invention is not however limited to visual display of the response. It may be possible for the response to be detected as a response outside the visible spectrum, for example in the infra-red or ultra-violet spectrum.
  • a blood sample applied to the sample collecting recess or cup of such a device did not appear to wet the member carrying the reagent composition, usually a membrane wall of the chamber opposite to the outlet of the capillary bore connection between the chamber and the sample collecting cup or recess. This was considered to be because the droplet formed at the outlet to the capillary bore did not readily detach from the outlet of the bore to fall onto or flow into contact with the membrane forming the opposed wall.
  • Various attempts were made to assist detachment of the droplet from the bore outlet, for example by tapping the device or by configuring the chamber so that the droplet wetted the side walls of the chamber and was drawn by surface tension effects onto the membrane wall.
  • the present invention provides a device for assessing a fluid sample, which device comprises: a. a fluid receptor means adapted to receive a sample of a fluid from an external source; b. a substantially closed chamber adapted to receive fluid from the receptor means; c. a member providing at least part of one wall of said chamber, said member carrying one or more reagents adapted to respond to one or more components of the fluid sample and adapted to give an indication of that response which can be detected from the exterior of the chamber; d.
  • the device is characterised in that at least the exposed rim of the sample receptor means is shaped so as to reduce the surface tension effects thereof with respect to the fluid to be assessed and/or the receptor means is provided with means to allow air to flow to and from the space above the fluid within the receptor means.
  • the receptor means is preferably a cup or recess having an open end having a circumferential rim.
  • a user places his finger carrying blood from a pin prick or other skin puncture in contact with the rim.
  • the user transfers the blood from the finger into the cup or recess by wiping the finger across the rim.
  • the surface tension properties of the rim can be decreased by forming the rim from or coating the rim with a material which is not readily wet by the blood, this may present problems in manufacturing the device. It is therefore preferred to modify the construction of the rim so that its surface tension effects are reduced mechanically. This can be achieved by forming the rim with discontinuities in its perimeter which serve to break the meniscus of the blood or other fluid in the receptor means at one or more points around the rim of the cup or recess.
  • air can be introduced into the cup above the surface of the fluid at or below the rim, for example by means of a vent hole through the wall of the device. This will allow air to flow into the cup or recess to break any vacuum which is drawn between the top surface of the fluid and the finger across the top of the cup or recess. This will allow the fluid to drain more readily from the cup or recess through the bore leading to the chamber of the device.
  • the discontinuities in the rim of the cup or recess may take a wide range of forms, for example a toothed or crenellated configuration to the rim.
  • the pitch of the teeth or crenellations are larger than can be spanned by the fluid under surface tension forces so that the fluid readily detaches from the rim, thus allowing the fluid in the cup or recess to flow into the bore leading to the chamber.
  • the discontinuities can be provided by the crests and troughs of a fluted or ribbed interior surface to the cup or recess which give a saw tooth rim to the cup or recess where the interior surface of the cup or recess intersects with the surface of the body of the device.
  • the projections may be axial as with a crenellated rim, or may be radial as when a fluted cup interior surface intersects with a flush surface of the body of the device.
  • the crenellations or saw tooth formations may project into the body of the device rather than project from it, as for example when radial saw cuts are made across the face of the device in whose plane the open end of the cup or recess lies.
  • the rim with a plurality of upstanding axial projections which serve to raise the user's finger out of direct contact with the rim and to break any meniscus which attempts to form at the rim of the cup or recess.
  • projections can be integrally formed during moulding of the main body of the device or can be in the form of short rods which are set into the rim or into the body of the device around the rim of the cup or recess during moulding of the body of the device.
  • the projections conveniently take the form of short cylindrical rods or studs having rounded exposed tips set into the body of the device adjacent the rim or into the rim of the cup or recess itself.
  • the projections are preferably from 0.5 to 5, typically from 1 to 2.5, mms long so as to keep the users skin clear of the surface of the rim at at least two places around the periphery of the rim.
  • the projections are located symmetrically around the perimeter of the rim, for example at from 15 to 120° intervals.
  • discontinuities in the rim of the cup or recess are present, these also serve the function of allowing air to flow in and out of the space above the fluid meniscus in the cup or recess. This allows the fluid to drain from the cup or recess into the underlying chamber without the formation of a vacuum between the user's finger and the fluid.
  • This venting also has the effect of minimising any pumping effect on the flow of fluid into the chamber when the user varies the pressure he applies in retaining his finger in place upon the rim of the cup or recess. Furthermore, the venting reduces the risk of aeration of the blood sample which may occur when such pumping occurs. Aeration of the sample may lead to inaccurate test results.
  • the admission of air into the space above the fluid in the receptor means may also be achieved by providing one or more vent passages through the side wall of the cup or recess and/or by forming the body of the device surrounding the cup or recess from a foraminous or porous material, for example from a fritted ceramic.
  • a foraminous or porous material for example from a fritted ceramic.
  • the receptor means communicates with the underlying chamber of the device by means of a bore.
  • This can be a conventional circular cross-section bore.
  • the bore may have a squared, triangular, cruciform or stellate cross-section, which may be formed during moulding of the device or by subsequent machining.
  • the interior face of the receptor means for example the cup or recess, and the bore a sand or bead blasted finish using conventional techniques to further reduce surface tension effects.
  • the bore can be of a squared, cruciform or stellate cross-section so as to provide a number of sharp turns in the outlet rim which serve to break the meniscus which forms at the outlet to the bore.
  • the rim can be formed as a sharp rim so as to minimise the contact area between the fluid and the outlet rim.
  • the device can be essentially identical to that described in our co-pending International Application.
  • Figure 1 is an axial cross- section through the device; and Figure 2 is an exploded perspective view of an alternative form of the device of Figure
  • the device of Figure 1 comprises a cylindrical housing member
  • the housing is formed with a sample receiving cup
  • the cup 2 connected to an axial capillary bore 3.
  • the cup 2 has a rim which carries a number of upstanding axial projections 4 about
  • the projections 4 can be lengths of rod inserted into the cup rim.
  • the projections 4 serve to prevent the formation of a continuous contact between the rim and the user's finger as a user draws his fingertip across the open mouth of the cup 2 so as to transfer a drop of blood into the cup 2.
  • a bore 3 connects the base of the cup 2 with a chamber 5 formed in a base of the device. Typically, the bore
  • the chamber 3 has a diameter of from 0.25 to 2.5 mms and the chamber has an axial depth of from 0.5 to 5 mms.
  • the bottom end of the bore terminates in a tapered edge 15 which helps the blood to pass from the bore into the chamber 5.
  • the base 11 is a second plastics moulding which has a floor 13 and side walls 14 and into which the housing 1 can be fitted, to form the chamber 5.
  • the floor 13 has an aperture 12.
  • a reagent pad 9 is mounted in the chamber 5 so that it extends across the aperture 12 and is clamped in place by the action of push-fitting the housing 1 into the base.
  • the chamber 5 is vented to the atmosphere by a labyrinthine vent passage indicated at 8.
  • the housing 1 and the base 11 are formed symmetrically about the longitudinal axis of the housing .
  • the reagent pad 9 can be in the form of a disc coated with a reagent on one face, or the reagent can be impregnated into the material of the disc.
  • the internal face of the cup 2 is fluted or carries radial ribs on its surface which extend to the rim.
  • the ribs or flutes form a saw toothed rim to the cup having a series of axial projections 4a as shown in Figure 2. It may be desired to blunten the crowns of the saw teeth to minimise the risk of injury to a user.
  • a user wipes his finger across the rim of cup 2 to transfer a drop of blood into the cup. Due to the projections 4 or 4a on the rim, a continuous circle of contact between the blood and the rim of the cup 2 is not formed. As a result, surface tension effects at the rim do not retain the droplet of blood and the blood readily travels along the bore 3 due to capillary action and/or under the influence of gravity to form a droplet at the outlet end of bore 3, as shown dotted in Figure 1.
  • the drop can readily detach from the outlet of bore 3 to fall onto the reagent pad 9 without the need to tap or "flick" the device, as might have been required where the rim does not carry the projections 4.
  • the blood sample is contained within chamber 5 and there is little risk of escape of the blood to contaminate the user, other samples or any test machine in which the response of the reagent is assessed.
  • the reagent responds to one or more of the components in the blood in the usual manner and this response can then be observed through the circular viewing aperture 12 from outside the container. Again, this response can be viewed without the need to remove the blood from chamber 5, further reducing the risk of cross-contamination.
  • the device can readily be mounted in a suitable receptacle in a response monitoring device so that the outer face of disc 9 can be observed.
  • the device can thus readily be handled mechanically where large numbers of samples are to be processed, or the device can be readily handled by a blind or infirm person to locate it in a monitoring device.
  • the invention thus also provides a method for testing a fluid sample for the presence of a component or property therein, which method is characterised in that the sample of fluid is applied to the receptor of a device of the invention, the fluid is allowed to flow through the bore to the chamber and to contact the reagent(s) carried by the wall thereof; and the response of the reagent(s) to the fluid is observed externally through the wall of the chamber.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention se rapporte à un dispositif servant à analyser un échantillon de fluide, et comprenant: a) un élément (2) destiné à recevoir un échantillon d'un fluide à partir d'une source externe; b) une chambre (5) pratiquement fermée et conçue pour recevoir un fluide à partir de l'élément de réception de fluide; c) un élément (9) constituant au moins une partie d'une paroi de ladite chambre, ledit élément (9) comportant un ou plusieurs réactifs conçus pour produire une indication de toute réponse pouvant être détectée de l'extérieur de la chambre (5); d) un passage (3) reliant l'élément de réception (2) de fluide et la chambre (5), et comprenant un orifice de sortie (15) au niveau de son extrémité contiguë à la chambre (5), le diamètre du passage étant choisi de façon que le fluide qui s'y trouve forme une gouttelette ou un ménisque au niveau de l'orifice de sortie (15) du passage. Cette goutelette ou ce ménisque va entrer en contact avec l'élément portant un réactif, le passage (3) étant conçu pour conduire le fluide de l'élément de réception (2) vers la chambre (5), et pour déposer le fluide sur une région prédéterminée de l'élément (9) portant le réactif. Le dispositif se caractérise en ce que le bord exposé de l'élément de réception d'échantillon, tout au moins, est modifié de façon à en réduire les effets de tension de surface par rapport au fluide devant être analysé, et/ou en ce que l'élément de réception est pourvu de moyens (4, 4a) permettant à l'air de s'écouler dans l'espace au-dessus du ménisque, ou à partir de cet espace, dans l'élément de réception (2).
PCT/GB1993/001797 1992-08-26 1993-08-24 Dispositif d'essai pour fluides WO1994004272A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU49686/93A AU4968693A (en) 1992-08-26 1993-08-24 Fluid testing device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929218118A GB9218118D0 (en) 1992-08-26 1992-08-26 Device
GB9218118.9 1992-08-26

Publications (1)

Publication Number Publication Date
WO1994004272A1 true WO1994004272A1 (fr) 1994-03-03

Family

ID=10720973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1993/001797 WO1994004272A1 (fr) 1992-08-26 1993-08-24 Dispositif d'essai pour fluides

Country Status (3)

Country Link
AU (1) AU4968693A (fr)
GB (1) GB9218118D0 (fr)
WO (1) WO1994004272A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018176412A (ja) * 2017-04-19 2018-11-15 天亮醫療器材股▲ふん▼有限公司ＳＫＹＬＡ Ｃｏｒｐｏｒａｔｉｏｎ 検出装置およびその注入口構造
US20220331797A1 (en) * 2017-02-06 2022-10-20 Efa - Engineering For All Ltd. Micromixer
US12023669B2 (en) * 2022-06-28 2024-07-02 Efa—Engineering For All Ltd. Micromixer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0010456A1 (fr) * 1978-10-25 1980-04-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Dispositif de transport d'un liquide
GB2139519A (en) * 1983-04-29 1984-11-14 Kenneth Dawson Bagshawe Reaction-chamber and filter for chemical analysis
EP0348006A2 (fr) * 1988-06-23 1989-12-27 Behring Diagnostics Inc. Appareil pour transférer des liquides et appareil d'analyse diagnostique
WO1992007655A1 (fr) * 1990-10-30 1992-05-14 Hypoguard (Uk) Limited Dispositif de recueil et de presentation
EP0503379A2 (fr) * 1991-03-11 1992-09-16 Bayer Corporation Dispositif d'essai avec un système de distribution d'un échantillon

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0010456A1 (fr) * 1978-10-25 1980-04-30 EASTMAN KODAK COMPANY (a New Jersey corporation) Dispositif de transport d'un liquide
GB2139519A (en) * 1983-04-29 1984-11-14 Kenneth Dawson Bagshawe Reaction-chamber and filter for chemical analysis
EP0348006A2 (fr) * 1988-06-23 1989-12-27 Behring Diagnostics Inc. Appareil pour transférer des liquides et appareil d'analyse diagnostique
WO1992007655A1 (fr) * 1990-10-30 1992-05-14 Hypoguard (Uk) Limited Dispositif de recueil et de presentation
EP0503379A2 (fr) * 1991-03-11 1992-09-16 Bayer Corporation Dispositif d'essai avec un système de distribution d'un échantillon

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
COLUMBUS: "architextured fluid management of biological liquids", CLINICAL CHEMISTRY, vol. 33, no. 9, 1987, US, pages 1531 - 1537 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220331797A1 (en) * 2017-02-06 2022-10-20 Efa - Engineering For All Ltd. Micromixer
US11865537B2 (en) 2017-02-06 2024-01-09 E.F.A. Engineering For All Ltd. Portable digital diagnostic device
JP2018176412A (ja) * 2017-04-19 2018-11-15 天亮醫療器材股▲ふん▼有限公司ＳＫＹＬＡ Ｃｏｒｐｏｒａｔｉｏｎ 検出装置およびその注入口構造
US12023669B2 (en) * 2022-06-28 2024-07-02 Efa—Engineering For All Ltd. Micromixer

Also Published As

Publication number Publication date
AU4968693A (en) 1994-03-15
GB9218118D0 (en) 1992-10-14

Similar Documents

Publication Publication Date Title
US4024857A (en) Micro blood collection device
JP3387649B2 (ja) 点着チップ
US5100620A (en) Capillary tube/gap reagent format
US5833630A (en) Sample collection device
US5366902A (en) Collection and display device
US4635488A (en) Nonintrusive body fluid samplers and methods of using same
US4660569A (en) Venting, automatic-stopping, aspirating plungers for syringes
US20030206828A1 (en) Whole blood sampling device
US6056925A (en) Sample vessel for taking blood samples
US4396024A (en) Device for the extraction of capillary blood
AU2358299A (en) Process for the production of analytical devices
DK157263B (da) Reaktionskuvette og reaktionsbakke bestaaende af et antal af saadanne reaktionskuvetter
US20220016617A1 (en) Sample collection and dispensing device
WO2008012566A2 (fr) Dispositif, système et procédé de collecte de liquide
JP3243619B2 (ja) 貯蔵タンクから血液を安全に取出す装置
JPS61284235A (ja) 微少量収集容器用血液収集装置
US8460617B2 (en) Pipette tip and a method for pipetting a congealed blood sample utilizing the pipette tip
JPH07284674A (ja) ピペットチップ
WO1994004272A1 (fr) Dispositif d'essai pour fluides
US3873449A (en) Filter skimming apparatus
CA2448803A1 (fr) Limitation de l'evaporation d'une reserve de fluide
JPH05184949A (ja) 微量定容ピペット
JPH02171Y2 (fr)
JPH06210187A (ja) 微量定容ピペット
JP6389870B2 (ja) 流体試料の収集用装置、装置を受けるレセプタクル、流体試料の収集用アセンブリ、及び流体試料を収集する方法

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AT AU BB BG BR CA CH CZ DE DK ES FI GB HU JP KP KR KZ LK LU MG MN MW NL NO NZ PL PT RO RU SD SE SK UA US VN

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA