WO1992022379A1 - Sonde de prelevement par pipette comprenant un entrainement reduit du reactif - Google Patents

Sonde de prelevement par pipette comprenant un entrainement reduit du reactif Download PDF

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Publication number
WO1992022379A1
WO1992022379A1 PCT/US1991/004203 US9104203W WO9222379A1 WO 1992022379 A1 WO1992022379 A1 WO 1992022379A1 US 9104203 W US9104203 W US 9104203W WO 9222379 A1 WO9222379 A1 WO 9222379A1
Authority
WO
WIPO (PCT)
Prior art keywords
liner
sheath
pipetting probe
polymeric
probe
Prior art date
Application number
PCT/US1991/004203
Other languages
English (en)
Inventor
Alfred James Claflin, Jr.
Original Assignee
Applied Research Systems Ars Holding N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems Ars Holding N.V. filed Critical Applied Research Systems Ars Holding N.V.
Priority to JP3514042A priority Critical patent/JPH06508428A/ja
Priority to EP91914994A priority patent/EP0588791A1/fr
Priority to CA002111355A priority patent/CA2111355A1/fr
Priority to PCT/US1991/004203 priority patent/WO1992022379A1/fr
Priority claimed from CA002111355A external-priority patent/CA2111355A1/fr
Publication of WO1992022379A1 publication Critical patent/WO1992022379A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G01N2035/1006—Rinsing only the inside of the tip

Definitions

  • Pipetting probes are generally used in automatic diagnostic or analytical equipment and serve to extract measured quantities of reagents of various types from the containers which store them and to dispense these reagents as required to conduct the test or analysis which is to be performed. After each extraction and dispensing of a particular reagent, the pipetting probe must be carefully washed, both inside and out, to remove all traces of that reagent in order to prevent contamination of the other reagents and carryover of that reagent into subsequent tests.
  • the present invention embraces an improved pipetting probe wherein a portion of the interior of said probe is etched so as to create fluid turbulence therewithin during use to improve the washing out of residual sample fluid.
  • the pipetting probe of the present invention comprises a rigid tubular sheath tightly circumscibing a tubular polymeric liner, wherein a portion of the interior of said polymeric liner is etched, particularly that portion excluding the sample region portion.
  • the present invention also embraces a method of improving the internal washing efficiency of a pipetting probe by etching a portion of the interior of the pipetting probe.
  • the invention embraces a method of making an improved pipetting probe by pulling a tubular polymeric liner, which has a narrowed liner portion formed by a narrowing die, into a rigid tubular sheath, and allowing the narrowed liner portion to expand against screw threads in the tip portion of the sheath so as to become immovably fixed therein.
  • Fig. 1 is a perspective view of the pipetting probe of the present invention and depicts both the rigid sheath, with its tip portion, and the polymeric liner.
  • Fig. 2a is an enlarged cross-section of the tip portion of the rigid sheath and depicts the threaded section (shown by dashed lines) and the polymeric liner portion.
  • Fig. 2b is an enlarged cross-section of the opposite end of the rigid sheath and depicts the polymeric liner portion tightly circumscribed by the main portion of the rigid sheath and extending out therefrom.
  • Fig. 2c is an even further enlarged cross-section of a fragment of the tip portion and depicts the threaded section therewithin.
  • Fig. 3 is a cross-section of a narrowing die and depicts a portion of polymeric liner being drawn therethrough. Description of the Preferred Embodiments
  • reagent and sample fluid are used interchangeably and are intended to include any fluid material used in a diagnostic or analytical test instrument.
  • fluid materials include the sample being tested, for example patient samples, such as blood or urine or extracts thereof, as well as standardized test samples, and the reagents utilized to conduct the test(s) to be performed, for example chemical, biochemical or biological reagents, including immunological reagents such as solutions of antibodies or antigens.
  • the present invention embraces a pipetting probe comprising a polymeric tube having a discharge end into or from which a quantity of sample fluid may be drawn or discharged, wherein a portion of the interior of said tube is etched so as to create fluid turbulence therewithin during use to improve the washing efficiency thereof.
  • a portion of the polymeric tube interior which is etched excludes at least about the first 20% of said tube at the discharge end, more preferably about the first 30% to 70% of said tube at the discharge end.
  • the pipetting probe will comprise a sample region portion which extends from the discharge end to a predetermined length of the polymeric tube. This is the portion which will hold a predetermined measured volume of sample fluid.
  • etching a portion of the polymeric tube interior substantially increases the washing efficiency of the probe interior. It is theorized that when wash solution is passed through the probe interior, it becomes turbulent as a result of contact with the etched interior wall. This turbulence then carries through the non-etched portion, which includes the sample region portion, and assists in the removal of any traces of sample fluid which otherwise might adhere to the smooth wall of the probe. Without such etching, the interior wall is substantially smooth throughout, which results in a substantially laminar flow of wash fluid through the probe (near-zero fluid velocity at the wall surface) .
  • the washing efficiency of the pipetting probe can be .further improved by altering the inner diameter of the probe in certain sections.
  • the tip portion of the polymeric tube i.e. at the discharge end
  • the polymeric tube will also have an intermediate portion, with an intermediate inner diameter, between the tip portion and the major part of the polymeric tube. This intermediate inner diameter is preferably about 20 to 30% narrower than the major part of the polymeric tube.
  • the pipetting probe 10 in its most practical form, comprises a rigid tubular sheath 11, preferably fabricated of stainless steel, and a tubular polymeric liner 12, preferably fabricated of polyfluoroethylene.
  • the rigid tubular sheath comprises a main portion 13 and a tip portion 14 at one end thereof, said main portion 13 having a first inner diameter and said tip portion 14 having a second inner diameter, with said second inner diameter being equal to or less than said first inner diameter.
  • said second inner diameter is less than said first inner diameter, most preferably about 25% less.
  • the tip portion 14 will have an outer diameter which is less than the outer diameter of the main portion 13 and will be connected to said main portion through angularly inclined neck portion 15, which serves as a transition between the tip portion and the main portion.
  • the tip portion 14 has a discharge end 16, into or from which sample fluid may be drawn or discharged.
  • This discharge end 16 is preferably cut at an angle (or beveled) to facilitate the puncturing of sealed cartridges and the dispensing of fluid. It is also especially preferred that a portion of the tip portion contain internal screw threads 20 for gripping the liner as will be discussed later.
  • the rigid tubular sheath is fabricated from 15 gauge type 304 stainless steel tubing and has the following dimensions:
  • the tubular polymeric liner 12 is the more critical part of the invention since that is the part which holds the sample fluid and which is the most difficult to wash of residual sample fluid after it is dispensed.
  • the polymeric liner is constructed of a flexible, inert, non-wettable material such as polyfluoroethylene. Its length and inner diameter are selected so as to hold a predetermined quantity of sample fluid as a minimum (i.e. the sample region portion) , plus some excess to serve as a buffer between the sample region portion and the operative machinery to which the liner is connected (e.g. Cavro syringe) .
  • the outer diameter of the polymeric liner should be approximately equal to or slightly greater than the inner diameter of the rigid sheath (particularly the main portion of the sheath) so that, upon insertion, it is tightly circumscribed and, preferably, immovably fixed therein. It will be longer than the rigid sheath and will extend from the discharge end 16, to which it is preferably cut flush, through and beyond the opposite end of the sheath.
  • the sheath to which the liner is inserted is the one previously described.
  • the polymeric liner utilized consists of polyfluoroethylene tubing (FEP tubing manufactured by
  • a portion of the liner is drawn through a narrowing die 17, as illustrated in Fig. 3, to provide a narrowed liner portion 18 of 1.07 mm (0.042 in) outer diameter and 0.41 mm (0.016 in) inner diameter.
  • the outer diameter of the narrowed liner portion 18 is somewhat less than the inner diameter of the tip portion 14, which is 1.13 mm (0.045 in), to facilitate easy insertion of the liner within the sheath. For this reason the length of the narrowed liner portion is advantageously greater than the length of the sheath.
  • the narrowed liner portion Upon insertion of the narrowed liner portion through the sheath, so that it extends through the discharge end thereof, it is pulled with the necessary force so as to draw the liner into the sheath, thereby narrowing that portion of the liner which enters the main portion of the sheath so as to form an intermediate liner portion 19 until that intermediate liner portion abuts against the interior wall of the neck portion.
  • the intermediate liner portion 19 will thus be tightly circumscribed by the main portion 13 of the rigid sheath and will have a length and outer diameter equal to the length and inner diameter of the main portion of the rigid sheath. The inner diameter of the intermediate liner portion is thus reduced to 0.71 mm (0.028 in).
  • the probe is then heated to about 200°F (93°C) for approximately two hours to allow the narrowed liner portion to expand (due to memory characteristics of the material) and become tightly circumscribed by the tip portion.
  • This expansion increases the inner diameter of the liner within the tip portion to 0.46 mm (0.018 in).
  • This expansion also causes the narrowed liner portion to expand against the screw threads 20 and become immovably fixed within the tip portion.
  • This is an important feature of the present invention since it substantially prevents movement of the liner away from the discharge end of the probe, which is a problem with conventionally lined probes.
  • the liner is cut flush with the discharge end of the sheath and to a suitable length for the intended use of the probe, which in this case is 585 mm (23.0 in) .
  • the liner is immovably fixed within the sheath, it is preferred to apply adhesive to either the interior of the sheath or the exterior of the liner prior to pulling the liner into the sheath, then subsequently curing the adhesive. It is most preferred to use an epoxy adhesive, such as Smooth-On MT-13. To insure good bonding of the adhesive to the polyfluoroethylene liner, the exterior surface of the liner which will come in contact with the adhesive should be etched with a chemical etchant, such as Chemgrip Treating Agent (Norton Company) .
  • the pipetting probe described above is intended to measure samples of approximately 100 ⁇ L to 225 ⁇ L volume. Accordingly, the sample region of the probe consists of about the first 335 mm ('"13 in) starting from the discharge end. The remainder of the probe, which is about 250 mm
  • the control probe is very similar to the inventive probe except that the polyfluoroethylene liner is not etched and has an inner diameter of 0.58 mm (0.023 in) at the tip portion and 1.07 mm (0.042 in) throughout the remainder of the liner.
  • the test used to measure sample carryover utilizes a sample well spiked with 1-2 x 10 mlU/ml of human chorionic gonadotropin (hCG) . Diagnostic testing of hCG is used to determine pregnancy. Generally, a measured hCG value above 25 mlU/ml is a positive indication of pregnancy.
  • test protocol for sample carryover used to compare the performance of the inventive probe versus the control probe involved the use of each probe in a conventional SRI diagnostic instrument set up to read hCG levels from several control cartridge wells containing 10 mlU/ml hCG, followed by a spiked well containing 1-2 x 10 mlU/ml hCG, followed by a control well.
  • the instrument was set to run its standard hCG assay under which the pipetting probe draws and dispenses 100 ⁇ l of hCG solution and is washed with about 1.4 ml (about 1.0-1.2 ml internally) of a buffered surfactant wash solution.
  • the hCG carryover from the spiked well is the difference between the control reading after the spike and the control reading before the spike.
  • the actual quantity of hCG carryover is converted to parts per million (that is, parts of hCG carried over per million parts in the spiked well) by dividing this difference by the amount of hCG in the spiked well.
  • control probe produces an hCG carryover in the range of 30-40 ppm.
  • probe of the present invention has been found to reduce the hCG carryover to less than 10 ppm, and generally to about 2-6 ppm. This represents approximately a ten-fold improvement.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Cette invention concerne une sonde améliorée de prélèvement par pipette dont une partie interne est mordancée de manière à créer une turbulence du fluide se trouvant à l'intérieur de ladite sonde pendant son utilisation, afin d'améliorer l'élimination du fluide d'échantillonnage résiduel. Plus spécifiquement, la sonde de prélèvement par pipette de cette invention comprend une enveloppe (11) tubulaire rigide qui entoure de manière serrée une doublure (12) polymère tubulaire dont une partie interne est mordancée, cette même partie ne comprenant pas la partie de la zone du prélèvement. Cette invention concerne également un procédé permettant d'améliorer l'efficacité du nettoyage interne d'une sonde de prélèvement par pipette grâce au mordançage d'une partie située à l'intérieur de ladite sonde. En outre, cette invention concerne un procédé de fabrication d'une sonde de prélèvement par pipette dans lequel on tire une doublure polymère tubulaire comprenant une partie de doublure rétrécie formée par une filière réductrice, dans une enveloppe tubulaire rigide et on laisse se dilater la partie de doublure rétrécie dans la partie d'extrémité de l'enveloppe pour qu'elle s'y fixe de manière définitive.
PCT/US1991/004203 1991-06-13 1991-06-13 Sonde de prelevement par pipette comprenant un entrainement reduit du reactif WO1992022379A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP3514042A JPH06508428A (ja) 1991-06-13 1991-06-13 試薬のキャリィオーバーを低減したピペッティングプローブ
EP91914994A EP0588791A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette comprenant un entrainement reduit du reactif
CA002111355A CA2111355A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette a retention de reactif reduite
PCT/US1991/004203 WO1992022379A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette comprenant un entrainement reduit du reactif

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002111355A CA2111355A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette a retention de reactif reduite
PCT/US1991/004203 WO1992022379A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette comprenant un entrainement reduit du reactif

Publications (1)

Publication Number Publication Date
WO1992022379A1 true WO1992022379A1 (fr) 1992-12-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/004203 WO1992022379A1 (fr) 1991-06-13 1991-06-13 Sonde de prelevement par pipette comprenant un entrainement reduit du reactif

Country Status (1)

Country Link
WO (1) WO1992022379A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0670497A1 (fr) * 1994-02-23 1995-09-06 Bayer Corporation Pipette avec détection de niveau de fluide
WO2001068258A1 (fr) * 2000-03-15 2001-09-20 American Cyanamid Company Pointe de pipette
EP1143181A3 (fr) * 2000-04-08 2003-10-01 Möller Feinmechanik GmbH & Co. KG Tuyau métallique avec recouvrement interne en matière plastique et son procédé de production
FR2874841A1 (fr) * 2004-09-07 2006-03-10 Commissariat Energie Atomique Dispositif de transfert d'elements contenus dans un liquide
EP2027446A2 (fr) * 2006-06-14 2009-02-25 Dionex Corporation Aiguille d'echantillonage et procedes de moulage qui y recourent

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2440725A (en) * 1944-09-04 1948-05-04 American Pipe & Constr Co Method of making composite plastic lined metal pipe
US3093162A (en) * 1959-06-29 1963-06-11 Duriron Co Plastic lined perforated metal tube
US3369426A (en) * 1964-11-30 1968-02-20 Teleflex Inc Plastic-lined conduit
US4017582A (en) * 1973-01-12 1977-04-12 Naumann John R Process for making a probe sheet
US4569165A (en) * 1982-12-21 1986-02-11 University Of Surrey Structural connections

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2440725A (en) * 1944-09-04 1948-05-04 American Pipe & Constr Co Method of making composite plastic lined metal pipe
US3093162A (en) * 1959-06-29 1963-06-11 Duriron Co Plastic lined perforated metal tube
US3369426A (en) * 1964-11-30 1968-02-20 Teleflex Inc Plastic-lined conduit
US4017582A (en) * 1973-01-12 1977-04-12 Naumann John R Process for making a probe sheet
US4569165A (en) * 1982-12-21 1986-02-11 University Of Surrey Structural connections

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0588791A4 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0670497A1 (fr) * 1994-02-23 1995-09-06 Bayer Corporation Pipette avec détection de niveau de fluide
WO2001068258A1 (fr) * 2000-03-15 2001-09-20 American Cyanamid Company Pointe de pipette
EP1143181A3 (fr) * 2000-04-08 2003-10-01 Möller Feinmechanik GmbH & Co. KG Tuyau métallique avec recouvrement interne en matière plastique et son procédé de production
FR2874841A1 (fr) * 2004-09-07 2006-03-10 Commissariat Energie Atomique Dispositif de transfert d'elements contenus dans un liquide
WO2006027532A1 (fr) * 2004-09-07 2006-03-16 Commissariat A L'energie Atomique Dispositif de transfert d'elements contenus dans un liquide
EP2027446A2 (fr) * 2006-06-14 2009-02-25 Dionex Corporation Aiguille d'echantillonage et procedes de moulage qui y recourent
EP2027446A4 (fr) * 2006-06-14 2011-03-09 Dionex Corp Aiguille d'echantillonage et procedes de moulage qui y recourent
US8313476B2 (en) 2006-06-14 2012-11-20 Dionex Corporation Sampling needle and methods of forming and using same

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