WO1992011092A1 - Fiole centrifuge a goulot incline - Google Patents

Fiole centrifuge a goulot incline Download PDF

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Publication number
WO1992011092A1
WO1992011092A1 PCT/US1991/009180 US9109180W WO9211092A1 WO 1992011092 A1 WO1992011092 A1 WO 1992011092A1 US 9109180 W US9109180 W US 9109180W WO 9211092 A1 WO9211092 A1 WO 9211092A1
Authority
WO
WIPO (PCT)
Prior art keywords
axis
bottle
rotor
cap
angle
Prior art date
Application number
PCT/US1991/009180
Other languages
English (en)
Inventor
John R. Wells
Original Assignee
E.I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Du Pont De Nemours And Company filed Critical E.I. Du Pont De Nemours And Company
Priority to JP50296092A priority Critical patent/JP2002517160A/ja
Priority to KR1019930701904A priority patent/KR930703078A/ko
Publication of WO1992011092A1 publication Critical patent/WO1992011092A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/02—Centrifuges consisting of a plurality of separate bowls rotating round an axis situated between the bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D1/00—Containers having bodies formed in one piece, e.g. by casting metallic material, by moulding plastics, by blowing vitreous material, by throwing ceramic material, by moulding pulped fibrous material, by deep-drawing operations performed on sheet material
    • B65D1/02—Bottles or similar containers with necks or like restricted apertures, designed for pouring contents
    • B65D1/0223—Bottles or similar containers with necks or like restricted apertures, designed for pouring contents characterised by shape
    • B65D1/023—Neck construction

Definitions

  • the present invention relates to a bottle for use in a centrifuge rotor, and in particular, to a centrifuge bottle having a canted neck.
  • FIG. 1 The bottle generally indicated by the reference character B includes a molded body portion M having a neck N with exterior threads T thereon.
  • the neck N has an annular seat S formed about its rim.
  • An O-ring seal G is receivable on the seat S and is there held in place by *a cap insert I.
  • the insert I is itself receivable within a threaded cap C.
  • the cap C is threadedly engageable with the threads T on the neck N thereby to secure the cap C to the body M.
  • Figure 2 illustrates the bottle B when the same is received in a cavity H of a centrifuge rotor R having an axis of rotation AR extending therethrough.
  • the rotor cavity H is circular in shape and has a predetermined diameter D.
  • the axis AH of the cavity defines a predetermined angle of inclination ⁇ with respect to the axis of rotation AR.
  • the seal G is challenged by the high pressure liquid (pressurized due to centrifugal force). The region of primary challenge is shown by the reference arrow X.
  • the present invention is directed toward a bottle for holding a liquid sample during centrifugation in a centrifuge rotor, the rotor being of the type having a circular rotor cavity therein and an axis of rotation extending centrally therethrough.
  • the rotor cavity has a diameter D.
  • the axis of the rotor cavity defines a predetermined angle of inclination ⁇ with respect to the axis of rotation.
  • the axis of the cavity is spaced a predetermined distance R from the axis of rotation in a plane perpendicular to the axis of rotor.
  • the bottle comprises a circular body portion and a neck portion formed integrally therewith.
  • the body portion has a diameter substantially equal to the diameter D of the cavity, while the neck portion has a predetermined dimension L associated therewith.
  • the body portion and the neck portion each have an axis therethrough with the axis of the neck defining a predetermined angle ⁇ with respect to the axis of the body.
  • the angle ⁇ lies in the range from ten (10) degrees to (100- ⁇ ) degrees.
  • the bottle further comprises a cap engageable with the neck portion, the cap having a predetermined diametrical dimension associated therewith.
  • the diametrical dimension of the cap is less than the dimension of a chord spaced r distance from the axis of rotation and subtended by an angle of (360/N) degrees in the plane perpendicular to the axis of rotor.
  • the distance r is the distance that the diametrical dimension of the cap lies from the axis of rotation when the bottle is received in the rotor. In practice the distance r is defined in accordance with the relationship:
  • the cap has a base and a sidewall thereon, with the sidewall of the cap defining a predetermined angle with respect to the base, the angle being substantially equal to (90 + 180/N) degrees.
  • Figure 1 is an exploded side-elevational view, in section, of a prior art bottle and multi-piece sealing arrangement
  • Figure 2 is an enlarged, fragmentary side elevational view, in section, of the bottle of Figure 1 while in use within a centrifuge rotor;
  • Figure 3 is a side elevational view, in section, illustrating a bottle in accordance with the present invention.
  • Figure 4 is a side elevational view, in section, of the bottle of Figure 3 while disposed within a cavity of a centrifuge rotor, illustrating the location of a liquid meniscus within a bottle of Figure 3 while under centrifugation;
  • Figure 5 is a full plan view of Figure 4; and Figure 6 is a view generally similar to Figure 5 illustrating a modification of the bottle cap in accordance with the present invention.
  • FIG. 3 shown is a side elevational view, in section, of a bottle generally indicated by the reference 10 in accordance with the present invention.
  • the bottle 10 is useful for holding a liquid sample F ( Figure 4) during centnfugation in a centrifuge rotor R of the type discussed in connection with Figure 2.
  • the bottle 10 comprises a circular body portion 12 having an outside diameter 14 substantially equal to the diameter D of the rotor cavity H.
  • the body portion 12 has an axis 16 extending therethrough.
  • a neck portion 18 is formed integrally with the body portion 12.
  • the neck portion 18 has an axis 20 extending therethrough.
  • the axis 20 of the neck 18 intersects with the axis 16 of the body 12 at a point of intersection 22.
  • the axis 20 of the neck 18 defines a predetermined angle ⁇ , indicated by the reference character 24, with respect to the axis 16 of the body 12.
  • the angle ⁇ 24 lies in the range from ten (10) degrees to (100- ⁇ ) degrees.
  • the optimum value of the angle ⁇ 24 is (90- ⁇ ) degrees.
  • the neck 18 has a predetermined length dimension 26 as indicated by the character L.
  • the dimension L 26 is measured along the axis 20 of the neck from the rim 28 of the bottle to the point of intersection 22.
  • the exterior surface of the neck 18 adjacent to the rim 28 is provided with threads 30.
  • a cap generally indicated by the reference character 34 is provided for engagement with the bottle 10.
  • the cap 34 comprises a planar base portion 36 having in interior surface 361 and an exterior surface 36B thereon.
  • a skirt 38 extends from the base 36.
  • the skirt 38 also has an interior surface 361 and an exterior surface 36E.
  • the interior surface 381 of the skirt 38 is threaded, as at 40, whereby the cap 34 is engageable with the threads 30 on the neck portion 18.
  • the base portion 36 of the cap 34 is generally circular in perimetrical configuration and has a predetermined diametrical dimension 36D associated therewith. A limit on the magnitude of the diametrical dimension 36D is discussed hereinafter.
  • the base 36 is preferably generally circular it should be understood that the base portion 36 may assume any convenient polygonal configuration. In such a case the diametrical dimension 36D corresponds to the diameter of the corresponding circumscribed circle.
  • the exterior surface 38E of the skirt 38 has in the preferred instance a generally cylindrical configuration. However the exterior surface 36E of the skirt 38 may be frustoconical in shape, as is illustrated in Figure 6.
  • the angle 42 defined between the exterior surface 38E of the skirt 38 and the exterior surface 36E of the base 36 is substantially equal to an angle of (90 + 180 N) degrees, where N is the number of cavities H disposed in the rotor R.
  • N is the number of cavities H disposed in the rotor R.
  • the inner surface of the of the cap 34 of the bottle 10 provides a static seal to prevent leakage of a sample from the bottle during normal handling prior to and subsequent to centnfugation.
  • the cap may be fabricated from any material structurally rigid yet sufficiently compliant to provide a static seal against the mouth 28 of the bottle 10.
  • a suitable material is polypropylene.
  • the bottle 10 is also fabricated from polypropylene.
  • the bottle 10 is used within a cavity H in the rotor R.
  • the diametrical dimension 36D of the cap 34 is selected such that when the bottle 10 is disposed within a cavity H the cap 34 lies fully within a sector bounded by radially extending sector boundary lines SBi, SB2.
  • the sector is defined in a plane perpendicular to the axis of rotation AR and subtends an angle of (360/N) degrees.
  • the lines SBi, SB 2 bisect webs W that lie between adjacent cavities H.
  • the magnitude of the diametrical dimension 36D may be expressed in terms of the dimension of a chord CH defined in the plane perpendicular to the axis of rotor and subtended by an angle of (360/N) degrees and spaced a distance r from the axis of rotation.
  • the distance r is defined in accordance with the relationship:
  • the diametrical dimension 36D must be less than or at most equal to the dimension of the chord CH.
  • a sample of liquid is introduced into the bottle 10 and the cap 34 threadedly secured thereto.
  • the bottle is introduced into the cavity H of the rotor R and the cover of the rotor (if one is used) is attached.
  • a normal 36N of the exterior surface 36E of the cap 36 is perpendicular to the axis of rotation AR. Care should be exercised to insure that the exterior surface 36E of the cap 34 is presented toward the axis of rotation AR. In some instances unless this orientation is achieved difficulty will be encountered in securing the cover to the rotor.
  • the volume efficiency of the bottle is one hundred percent (100%). That is the entire available volume of the bottle 10 is occupied by a liquid sample.
  • a 250 mL "Oak Ridge-type" bottle mentioned earlier is used in a rotor such as a GSA rotor sold by the Biotechnology Systems Division of E. I. du Pont de Nemours and Company, (angle ⁇ equal to twenty-eight (28) degrees) then only sixty percent (60%) of the available volume of the bottle may be used if no challenge is to be presented to the seal by the liquid.
  • both the bottle in accordance with the invention and the prior art bottle are equal in terms volume efficiency one hundred percent (100%).
  • seal reliability is improved in a bottle in accordance with the present invention. Owing to the inclination of the neck the component of centrifugal force of the cap that contributes to sealing the bottle is increased. The tendency of the O-ring to become unevenly distributed around the mouth of the bottle in response to centrifugal force is also reduced.
  • the angle oc 24 lies in the range from ten (10) degrees to (100- ⁇ ) degrees with the optimum value of the angle ⁇ 24 being (90- ⁇ ) degrees. Since most rotors have an angle of inclination ⁇ in the range from twenty (20) to forty (40) degrees, the value of the angle oc 24 is in the range ten (10) to eighty (80) degrees.
  • the lower limit of the angle ⁇ 24 is the value at which the component of centrifugal force that contributes to sealing the bottle first becomes significantly greater than the component contributing to sealing a straight neck bottle.
  • the upper limit of the angle ⁇ 24 is the value at which the volume efficiency advantages of the bottle 10 begin to diminish.
  • cap 34 having the frustoconical skirt 38 may be used.
  • a rotor loaded with a full complement of bottles having such caps is shown in Figure 6.
  • the exterior surface 38E of adjacent bottles 10 are disposed in close proximity such that rotation of a bottle about the axis 16 of its body is prevented.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ceramic Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Centrifugal Separators (AREA)

Abstract

L'invention concerne une fiole (10) destinée à contenir un échantillon de liquide pendant une centrifugation dans un rotor centrifuge (R) présentant une cavité rotorique (H) définissant un angle prédéterminé d'inclinaison β par rapport à l'axe de rotation (AR). La fiole (10) comprend une partie de corps (12) présentant une partie de goulot (18) solidaire, un axe (20) à travers le goulot définissant un angle prédéterminé α par rapport à un axe (16) du corps.
PCT/US1991/009180 1990-12-21 1991-12-18 Fiole centrifuge a goulot incline WO1992011092A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP50296092A JP2002517160A (ja) 1990-12-21 1991-12-18 傾斜した首部を有する遠心分離用ボトル
KR1019930701904A KR930703078A (ko) 1990-12-21 1991-12-18 경사진 목부를 갖는 원심분리기용 병

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63143790A 1990-12-21 1990-12-21
US631,437 1990-12-21

Publications (1)

Publication Number Publication Date
WO1992011092A1 true WO1992011092A1 (fr) 1992-07-09

Family

ID=24531200

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/009180 WO1992011092A1 (fr) 1990-12-21 1991-12-18 Fiole centrifuge a goulot incline

Country Status (5)

Country Link
EP (1) EP0562010A4 (fr)
JP (1) JP2002517160A (fr)
KR (1) KR930703078A (fr)
IE (1) IE914479A1 (fr)
WO (1) WO1992011092A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5558616A (en) * 1995-09-07 1996-09-24 E. I. Du Pont De Nemours And Company Centrifuge rotor cover having container supports thereon
WO1998048941A1 (fr) * 1997-04-25 1998-11-05 Beckman Coulter, Inc. Ensemble capsule et fermeture monobloc solidaire autoclave charge par centrifugation
US6666346B2 (en) * 2002-01-15 2003-12-23 Plastipak Packaging, Inc. Blow molded plastic container with inclined mouth and method and apparatus for obtaining same
US9114407B2 (en) 2009-06-30 2015-08-25 Hitachi Koki Co., Ltd. Centrifugal separator with rotor having plurality of triangular-shaped holding cavities and rotor for use in centrifugal separator

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4941881B2 (ja) * 2006-02-23 2012-05-30 日立工機株式会社 遠心分離用ロ−タおよび遠心機
EP2190581B1 (fr) 2007-08-21 2012-12-05 Nalge Nunc International Corporation Fermeture de flacon à centrifuger et ensemble associé
JP5333760B2 (ja) * 2009-06-30 2013-11-06 日立工機株式会社 遠心分離機用試料容器

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2958461A (en) * 1959-02-19 1960-11-01 Dorr Oliver Inc Centrifuge machine
US4572412A (en) * 1983-08-09 1986-02-25 Zeller Plastik Koehn, Graebner & Co. Container made of plastic which can be elastically deformed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3366320A (en) * 1965-07-21 1968-01-30 Atomic Energy Commission Usa Centrifuge sample holder

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2958461A (en) * 1959-02-19 1960-11-01 Dorr Oliver Inc Centrifuge machine
US4572412A (en) * 1983-08-09 1986-02-25 Zeller Plastik Koehn, Graebner & Co. Container made of plastic which can be elastically deformed

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0562010A4 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5558616A (en) * 1995-09-07 1996-09-24 E. I. Du Pont De Nemours And Company Centrifuge rotor cover having container supports thereon
WO1997009118A1 (fr) * 1995-09-07 1997-03-13 Sorvall Products, L.P. Couvercle de rotor de centrifugeuse avec supports de recipients
WO1998048941A1 (fr) * 1997-04-25 1998-11-05 Beckman Coulter, Inc. Ensemble capsule et fermeture monobloc solidaire autoclave charge par centrifugation
US6666346B2 (en) * 2002-01-15 2003-12-23 Plastipak Packaging, Inc. Blow molded plastic container with inclined mouth and method and apparatus for obtaining same
US9114407B2 (en) 2009-06-30 2015-08-25 Hitachi Koki Co., Ltd. Centrifugal separator with rotor having plurality of triangular-shaped holding cavities and rotor for use in centrifugal separator

Also Published As

Publication number Publication date
EP0562010A4 (en) 1994-08-10
IE914479A1 (en) 1992-07-01
JP2002517160A (ja) 2002-06-11
KR930703078A (ko) 1993-11-29
EP0562010A1 (fr) 1993-09-29

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