WO1992003559A3 - Testsystem zur aktivitätsüberprüfung viraler proteinasen - Google Patents

Testsystem zur aktivitätsüberprüfung viraler proteinasen Download PDF

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Publication number
WO1992003559A3
WO1992003559A3 PCT/EP1991/001595 EP9101595W WO9203559A3 WO 1992003559 A3 WO1992003559 A3 WO 1992003559A3 EP 9101595 W EP9101595 W EP 9101595W WO 9203559 A3 WO9203559 A3 WO 9203559A3
Authority
WO
WIPO (PCT)
Prior art keywords
checking
activity
test system
viral proteinases
viral
Prior art date
Application number
PCT/EP1991/001595
Other languages
English (en)
French (fr)
Other versions
WO1992003559A2 (de
Inventor
Wolfgang Sommergruber
Hans-Dieter Liebig
Timothy Skern
Marion Luderer
Dieter Blass
Ernst Kuechler
Heinrich Kowalski
Ljerka Frasel
Herbert Auer
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19904027153 external-priority patent/DE4027153A1/de
Priority claimed from DE19914112952 external-priority patent/DE4112952A1/de
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Priority to JP91513892A priority Critical patent/JPH05506999A/ja
Publication of WO1992003559A2 publication Critical patent/WO1992003559A2/de
Publication of WO1992003559A3 publication Critical patent/WO1992003559A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22029—Picornain 2A (3.4.22.29)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Gegenstand der vorliegenden Erfindung ist ein in-vivo Farbtestsystem zur Überprüfung der Aktivität viraler Proteasen.
PCT/EP1991/001595 1990-08-28 1991-08-22 Testsystem zur aktivitätsüberprüfung viraler proteinasen WO1992003559A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP91513892A JPH05506999A (ja) 1990-08-28 1991-08-22 ウイルスプロティナーゼの活性をモニターするためのテスト系

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19904027153 DE4027153A1 (de) 1990-08-28 1990-08-28 Testsystem zur aktivitaetsueberpruefung viraler proteinasen
DEP4027153.6 1990-08-28
DEP4112952.0 1991-04-20
DE19914112952 DE4112952A1 (de) 1991-04-20 1991-04-20 Testsystem zur aktivitaetsueberpruefung viraler proteinasen

Publications (2)

Publication Number Publication Date
WO1992003559A2 WO1992003559A2 (de) 1992-03-05
WO1992003559A3 true WO1992003559A3 (de) 1992-04-30

Family

ID=25896304

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/001595 WO1992003559A2 (de) 1990-08-28 1991-08-22 Testsystem zur aktivitätsüberprüfung viraler proteinasen

Country Status (4)

Country Link
EP (1) EP0497949A1 (de)
JP (1) JPH05506999A (de)
CA (1) CA2072154A1 (de)
WO (1) WO1992003559A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965124A (en) * 1991-12-06 1999-10-12 Whitehead Institute For Biomedical Research Replication-competent recombinant viral vaccines and method of producing same
MX9206981A (es) * 1991-12-06 1994-08-31 Whitehead Biomedical Inst Vacunas de recombinantes y metodo para producirlas.
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
ES2170937T3 (es) * 1996-01-26 2002-08-16 Roche Diagnostics Corp Agentes de reticulacion bis-maleimido.
WO1999006072A1 (en) * 1997-07-30 1999-02-11 Boehringer Mannheim Corporation Cyclized prodrugs
EP1141419A4 (de) * 1998-12-24 2002-03-20 Small Molecule Therapeutics In Verfahren und zusammensetzungen zum identifizieren von modulatoren von protease
WO2007106456A2 (en) 2006-03-13 2007-09-20 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions using a reduced affinity enzyme complementation reporter system
US8101373B2 (en) 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
EP2724158B1 (de) 2011-06-23 2016-03-23 DiscoveRx Corporation Überwachung eines proteinverkehrs mit beta-galactosidase-reporter-fragmentkomplementierung

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2712615A1 (de) * 1977-03-18 1978-09-21 Max Planck Gesellschaft Verfahren zur herstellung von filamentoesen phagen als vektor fuer synthetische rekombinate
EP0321973A2 (de) * 1987-12-23 1989-06-28 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression der viral kodierten Protease P2A des HRV2
DE3819846A1 (de) * 1988-06-10 1989-12-14 Hans Prof Dr Dr Wolf Testsystem fuer die entwicklung von inhibitoren der protease von hiv
WO1990001061A1 (de) * 1988-07-25 1990-02-08 Boehringer Ingelheim International Gmbh In vitro synthese einer infektiösen rna

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2712615A1 (de) * 1977-03-18 1978-09-21 Max Planck Gesellschaft Verfahren zur herstellung von filamentoesen phagen als vektor fuer synthetische rekombinate
EP0321973A2 (de) * 1987-12-23 1989-06-28 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression der viral kodierten Protease P2A des HRV2
DE3819846A1 (de) * 1988-06-10 1989-12-14 Hans Prof Dr Dr Wolf Testsystem fuer die entwicklung von inhibitoren der protease von hiv
WO1990001061A1 (de) * 1988-07-25 1990-02-08 Boehringer Ingelheim International Gmbh In vitro synthese einer infektiösen rna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Gene, Band 60, 1987, Amsterdam (NL), S. Jia et al.: "Carbohydrate-binding protein 35: molecular cloning and expression of a recombinant polypeptide with lectin activity", Seiten 197-204, siehe Zusammenfassung; Seite 201, unter d) *
J. Sambrook et al.: "Molecular cloning", 2. Ausgabe,1989, Cold Spring Harbor Laboratory Press (US), Seiten 18.81 - 18.88, siehe Seite 18.81, 2. Absatz *
Proc. Natl. Acad. Sci., Band 88, Juli 1991, Washington (US), H.-D. Liebig et al.: "Proteinase trapping: Screening for viral proteinase mutants by alpha-complementation" Seiten 5979-5983, siehe das ganze Dokument *
Science, Band 206, 19. Oktober 1979, Lancaster (US), A. Van Ooyen et al.: "Comparison of total sequence of a cloned rabbit beta-globin ene and its flanking regions with a homologous mouse sequence", Seiten 337-344, siehe Abbildung 3, Position 1 (In der Anmeldung erwähnt) *

Also Published As

Publication number Publication date
JPH05506999A (ja) 1993-10-14
CA2072154A1 (en) 1992-03-01
WO1992003559A2 (de) 1992-03-05
EP0497949A1 (de) 1992-08-12

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