WO1992000039A1 - Procede et instrument pour l'examen cytologique des liquides organiques et des echantillons preleves par aspiration a l'aide d'une aiguille fine - Google Patents

Procede et instrument pour l'examen cytologique des liquides organiques et des echantillons preleves par aspiration a l'aide d'une aiguille fine Download PDF

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Publication number
WO1992000039A1
WO1992000039A1 PCT/SE1991/000468 SE9100468W WO9200039A1 WO 1992000039 A1 WO1992000039 A1 WO 1992000039A1 SE 9100468 W SE9100468 W SE 9100468W WO 9200039 A1 WO9200039 A1 WO 9200039A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
brush
instrument
filter body
cells
Prior art date
Application number
PCT/SE1991/000468
Other languages
English (en)
Inventor
Nils Stormby
Original Assignee
Nils Stormby
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nils Stormby filed Critical Nils Stormby
Publication of WO1992000039A1 publication Critical patent/WO1992000039A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2217/00—General characteristics of surgical instruments
    • A61B2217/002—Auxiliary appliance
    • A61B2217/005—Auxiliary appliance with suction drainage system

Definitions

  • the procedure has two drawbacks: firstly, some of the cell material sucked out by aspiration will often enter the syringe proper and can only with difficulty be removed therefrom, which means loss of cells and risk of reduced diagnostic accuracy, and secondly, the smearing of the aspirate presently must be performed immediately by the physician who has performed the operation, thin and even smearing of the cell material without crushing being important. In inexperienced hands, however, the material often becomes defect due to incorrect treatment. A defect cell material will, of course, influence the diagnostic precision to a great extent.
  • US-A-4366822 discloses a device where bone marrow is aspirated by means of a syringe comprising a filter chamber wherein the bone marrow is filtered through a filter on which particles from the bone marrow are deposited. The particles can then be scraped off by means of a scalpel or a knife to be transfered to a glass slide.
  • the invention eliminates the disadvantage and the risk of post-treating biological fluids and considerably reduces the cost of transport.
  • the purpose of the invention is also to overcome the disadvantages mentioned above.
  • the invention refers to a method and an instrument for collecting and concentrating cells in a fluid.
  • fluid refers to fluids normally occuring in the body (urine, saliva, bronchus secretion, spinal fluid etc), effusions of fluids resulting from disease (effusions
  • the presently applied method of handling fluids containing cells as mentioned above is either to deliver the fluid in its entirety to a laboratory where it is
  • FIG. 1 is a side view of an instrument according to the invention for sampling a tissue region in aspiration cytology
  • FIG. 2 is an enlarged side view of a container forming part of the instrument, having a filter body consisting of a brush inside the container which is parted for removal of the brush therefrom
  • FIG. 3 is a side view of the opened container, the brush being withdrawn therefrom by means of a special tool
  • FIG. 4 is a side view which shows how the material collected in the brush is smeared on a glass slide
  • FIG. 5 is a fragmentary side view, partly an axial cross section of a modified embodiment of the instrument for aspiration of fluid.
  • FIG. 1 shows a syringe 10 which can be quite conventional and has an outlet socket 11 forming a Luer type coupling piece.
  • a cylindrical container 12 is at one end thereof connected to said coupling piece, the container at the other end thereof having a Luer type coupling piece 13 for attachment of a thin cannula 14.
  • the container 12 comprises two parts, a larger one 12A and a smaller one 12B, which can be detachably interconnected or can be permanently joined in such a way that part 12B can be broken or twisted off from part 12B, as illustrated in FIG. 2.
  • a filter body 15 which in this case comprises a brush, suitably a brush of the type used for cervical sampling and including a stem 16 of twisted wires with bristles 17 mounted therein.
  • Such a brush is disclosed and described in US-A-4 759 376.
  • the brush can also be manufactured in another way and can for example consist of a body with velour finished surface according to WO 89/10724, and the brush can also be replaced by a wad of cotton or similar material. It is essential that the filter body substantially fills the container 12 completely.
  • the syringe 10 When the cannula 14 has been inserted into the organism from which a sample shall be taken, for example a tissue region 18 in an organ or a pathological trans ⁇ formation in an organ from which a fluid, i.e. liquid and accompanying cells (inclusive cell fragments), shall be aspirated, the syringe 10 is operated to suck up the fluid into the syringe.
  • the fluid passes through the cannula 14 and the container 12 and from there into the syringe 10, the fluid when passing through the container 12 being forced to follow the labyrinth-like paths between the bristles of the brush 15, which implies that cells and cell fragments will be collected in the brush which functions as a filter.
  • a suitable handle 19 can now be attached either by connecting the stem 16 with light forced fit to the handle, or by the handle being provided with a chuck or the like for fastening the stem 16 to the handle.
  • the brush is then withdrawn from the container part 12A, as illustrated in FIG. 3, and the cells and cell fragments collected in the brush can now, according to FIG. 4, be smeared on a glass slide 20 in the manner commonly used for cervical sampling by means of a brush of the kind described above.
  • the fluid can also consist of body fluid or liquid for irrigation as described above, which is sucked directly from the organism or from a vessel in which the liquid has been collected into a syringe or another collecting device through the container 12 receiving the brush 15. Alternatively, during tapping from the organism the liquid may be passed through said container.
  • FIG. 5 discloses an instrument for aspiration of body fluids, liquids for irrigation and the like, wherein a rigid or flexible, preferably transparent tube (hose) 21 at one end thereof is connected to a syringe 10 or a Luer type coupling piece 11, a brush 15 being introduced into the tube from the other end thereof, the stem 16 of the brush suitably projecting from latter end of the tube so as to be gripped when withdrawn from the tube.
  • a rigid or flexible, preferably transparent tube (hose) 21 at one end thereof is connected to a syringe 10 or a Luer type coupling piece 11, a brush 15 being introduced into the tube from the other end thereof, the stem 16 of the brush suitably projecting from latter end of the tube so as to be gripped when withdrawn from the tube.
  • the brush can in all embodiments of the invention comprise a brush with bristles which are mounted to a stem, or with bristles which are applied on the stem by flocculation, but the brush can also be replaced by a wad of cotton or similar material with or without a stem.
  • the container 12 can serve as a storage and transport package for the brush (wad) enclosed therein with cells collected on the brush and separated from the liquid for delivery to the laboratory where the sample obtained is to be examined and diagnosed.
  • the addition of a liquid protein may be appropriate.
  • an antibiotic may be recommended.
  • the addition may take place in the container or in the tube.
  • Another alternative consists in the brush or wad being treated with protein medium or antibiotic.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Filtering Materials (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procédé de rassemblement et de concentration des cellules dans un fluide tel qu'un liquide organique, un liquide d'irrigation ou une matière cytologique aspirée. On fait passer le liquide à travers une brosse ou un tampon d'ouate dans le passage d'écoulement de liquide, afin de rassembler les cellules existantes sur le corps filtrant. Les cellules se déposent sur une lame en verre. On a également prévu un instrument de mise en ÷uvre du procédé, comportant un élément qui forme un passage pour le liquide, et possédant une brosse (15) ou un tampon en ouate placé(e) dans ce passage pour servir de corps filtrant, ledit passage étant raccordé à un dispositif collecteur (10) du liquide afin de filtrer celui-ci lors de son passage vers le dispositif collecteur.
PCT/SE1991/000468 1990-06-29 1991-06-28 Procede et instrument pour l'examen cytologique des liquides organiques et des echantillons preleves par aspiration a l'aide d'une aiguille fine WO1992000039A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9002293-0 1990-06-29
SE9002293A SE466785B (sv) 1990-06-29 1990-06-29 Saett och instrument foer cytologisk undersoekning av kroppsvaetskor och finnaalsaspirat

Publications (1)

Publication Number Publication Date
WO1992000039A1 true WO1992000039A1 (fr) 1992-01-09

Family

ID=20379899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1991/000468 WO1992000039A1 (fr) 1990-06-29 1991-06-28 Procede et instrument pour l'examen cytologique des liquides organiques et des echantillons preleves par aspiration a l'aide d'une aiguille fine

Country Status (3)

Country Link
AU (1) AU8209291A (fr)
SE (1) SE466785B (fr)
WO (1) WO1992000039A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008561A1 (fr) * 1996-08-29 1998-03-05 Rafael Tovar Gutierrez Nouvelle aiguille pour produits injectables
WO2008020439A2 (fr) 2006-08-17 2008-02-21 Sialo Technology Israel Ltd Manche microscopique optique intégral
WO2013128177A1 (fr) * 2012-02-27 2013-09-06 Exmoor Innovations Ltd Dispositif et procédé
US8968210B2 (en) 2008-10-01 2015-03-03 Covidien LLP Device for needle biopsy with integrated needle protection
US9186128B2 (en) 2008-10-01 2015-11-17 Covidien Lp Needle biopsy device
US9782565B2 (en) 2008-10-01 2017-10-10 Covidien Lp Endoscopic ultrasound-guided biliary access system
US11298113B2 (en) 2008-10-01 2022-04-12 Covidien Lp Device for needle biopsy with integrated needle protection

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3224434A (en) * 1962-11-06 1965-12-21 Waldemar Medical Res Foundatio Cell collector
US4366822A (en) * 1979-12-03 1983-01-04 Applied Medical Devices, Inc. Method and apparatus for bone marrow cell separation and analysis
US4620548A (en) * 1980-04-21 1986-11-04 Accupap, Inc. Pap smear T-zone sampler

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3224434A (en) * 1962-11-06 1965-12-21 Waldemar Medical Res Foundatio Cell collector
US4366822A (en) * 1979-12-03 1983-01-04 Applied Medical Devices, Inc. Method and apparatus for bone marrow cell separation and analysis
US4620548A (en) * 1980-04-21 1986-11-04 Accupap, Inc. Pap smear T-zone sampler

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008561A1 (fr) * 1996-08-29 1998-03-05 Rafael Tovar Gutierrez Nouvelle aiguille pour produits injectables
WO2008020439A2 (fr) 2006-08-17 2008-02-21 Sialo Technology Israel Ltd Manche microscopique optique intégral
US8968210B2 (en) 2008-10-01 2015-03-03 Covidien LLP Device for needle biopsy with integrated needle protection
US9186128B2 (en) 2008-10-01 2015-11-17 Covidien Lp Needle biopsy device
US9782565B2 (en) 2008-10-01 2017-10-10 Covidien Lp Endoscopic ultrasound-guided biliary access system
US9913630B2 (en) 2008-10-01 2018-03-13 Covidien Lp Device for needle biopsy with integrated needle protection
US10076316B2 (en) 2008-10-01 2018-09-18 Covidien Lp Needle biopsy device
US10888689B2 (en) 2008-10-01 2021-01-12 Covidien Lp Endoscopic ultrasound-guided biliary access system
US11298113B2 (en) 2008-10-01 2022-04-12 Covidien Lp Device for needle biopsy with integrated needle protection
WO2013128177A1 (fr) * 2012-02-27 2013-09-06 Exmoor Innovations Ltd Dispositif et procédé
US20150050683A1 (en) * 2012-02-27 2015-02-19 Exmoor Innovations Ltd. Device And Method
CN104487003A (zh) * 2012-02-27 2015-04-01 埃克斯穆尔创新有限公司 装置和方法

Also Published As

Publication number Publication date
SE466785B (sv) 1992-04-06
SE9002293D0 (sv) 1990-06-29
AU8209291A (en) 1992-01-23
SE9002293L (sv) 1991-12-30

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