WO1991016074A1 - Administration d'anticorps monoclonaux contre des virus respiratoires - Google Patents

Administration d'anticorps monoclonaux contre des virus respiratoires Download PDF

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Publication number
WO1991016074A1
WO1991016074A1 PCT/US1991/002668 US9102668W WO9116074A1 WO 1991016074 A1 WO1991016074 A1 WO 1991016074A1 US 9102668 W US9102668 W US 9102668W WO 9116074 A1 WO9116074 A1 WO 9116074A1
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WO
WIPO (PCT)
Prior art keywords
mab
monoclonal antibody
administered
virus
respiratory
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Application number
PCT/US1991/002668
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English (en)
Inventor
James F. Young
Gregory A. Prince
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Medimmune, Inc.
Henry M. Jackson Foundation For The Advancement Of Military Medicine
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Application filed by Medimmune, Inc., Henry M. Jackson Foundation For The Advancement Of Military Medicine filed Critical Medimmune, Inc.
Publication of WO1991016074A1 publication Critical patent/WO1991016074A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1027—Paramyxoviridae, e.g. respiratory syncytial virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • This invention relates to the prophylaxis and/or treatment of respiratory viruses. More particularly, this invention relates to the prophylaxis and/or treatment of respiratory viruses through the administration of monoclonal antibodies against a respiratory virus .
  • Walsh, et al. , Infect, and Immun. . Vol. 43, pgs. 756-758 discloses the intraperitoneal administration of monoclonal antibodies against glycoproteins of respiratory syncytial virus to cotton rats, followed by an intranasal challenge of respiratory syncytial virus three hours after administration of the monoclonal antibody.
  • Such treatment with the monoclonal antibodies resulted in the reduction of viral titers or in the absence of virus in the lungs of the cotton rats which were given the monoclonal antibodies, while virus was detected in the lungs of all rats in the control group.
  • the treated group of rats also showed a decrease in virus growth in nasal turbinates.
  • USSR Patent No. 745,523 (1978) discloses the intranasal administration of secretory IgA antibodies, isolated from female colostrum, to a group of infants in a children's hospital, prior to an outbreak of respiratory syncytial virus. Those infants who were given the antibodies prior to the outbreak contracted less severe cases of respiratory syncytial virus and had developed fewer complications associated with the virus , than those infants who were not administered the antibodies.
  • U. S . Patent No. 4,800,078, issued to Prince, et al. discloses a method of treating respiratory disease caused by respiratory syncytial virus (RSV) by topically administering RSV antibodies into the lower respiratory tract.
  • RSV respiratory syncytial virus
  • the antibodies are administered as a purified human gamma globulin, and may be administered in an amount of from about 0.025 to 0.05g/kg of body weight.
  • the purified human gamma globulin preparation may contain RSV neutralizing antibodies at a titer greater than 1 :2 ,000.
  • a process for the prophylaxis of at least one respiratory virus in an animal comprising topically administering to the animal at least one monoclonal antibody against the at least one respiratory virus.
  • the at least one monoclonal antibody is administered to the respiratory tract.
  • Such administration may be by intranasal administration or by breathing an aerosol containing said at least one monoclonal antibody.
  • the animal is a human animal.
  • the at least one monoclonal antibody is also a neutralizing antibody.
  • Representative respiratory viruses include respiratory syncytial viruses, or RSV, parainfluenza viruses (types 1, 2, and 3) , influenza A viruses, influenza B viruses, and adenoviruses .
  • the at least one respiratory virus is a respiratory syncytial virus, and the monoclonal antibody or antibodies administered are preferably against a fusion protein of RSV.
  • monoclonal antibodies against a fusion protein of respiratory syncytial virus include MAb 1436C , MAb 1153 , MAb 1142 , MAb 151, MAb 1200, MAb 1214, MAb 1237, MAb 1129, MAb 1121 , MAb 1107, MAb 13-1 , MAb 43-1 , MAb 1112 , MAb 1269, MAb 1243 , MAb 1331H, MAb 1308F, MAb 1302 A.
  • Such antibodies are neutralizing antibodies.
  • Particularly preferred monoclonal antibodies are MAb 1121 , MAb 1129, MAb 1142 , MAb 1153 , MAb 1243, MAb 1302A, MAb 1308F, and MAb 1331H.
  • monoclonal antibodies as hereinabove described may be employed in the prophylaxis of respiratory syncytial virus.
  • These antibodies have been characterized with regard to their neutralizing potential, both to parental RSV strains used to immunize mice to generate these antibodies, as well as a number of different virus strains isolated from 1956 to 1985 from various geographical locations.
  • These antibodies have also been mapped by competitive binding and reactivity profiles of virus escape mutants to three broad antigenic sites (A, B, and C) containing 16 distinct epitopes . Epitopes within antigenic sites A and C show the least variability in natural isolates.
  • Such monoclonal antibodies are further described in Beeler, et al. , J. Virol. , Vol. 63, No. 7, pgs. 2941-2950 (July, 1989) .
  • non-neutralizing monoclonal antibodies against a fusion protein of respiratory syncytial virus include MAb 1224-1, MAb 1175-40, MAb 1105-1, MAb 1219-22 , MAb 1228-28, and MAb 1113-44. These monoclonal antibodies do not neutralize virus infectivity in vitro; however, these antibodies prevent virus replication in cells in vivo . These antibodies may be obtained from Dr. Judy A. Beeler, of the Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
  • monoclonal antibodies against parainfluenza viruses include MAb 271/7, MAb 170/7, MAb 423/4, MAb 61/5, MAb 403/7 , MAb 77/5 , MAb 447/12 , MAb 101/1, MAb 128/9, MAb 454/11, MAb 149/3, MAb 429/5, MAb 66/4, MAb 68/2, MAb 451/4 and MAb 166/11.
  • Such monoclonal antibodies recognize one of 10 epitopes of the hemagglutinin-neuraminidase (HN) protein of human parainfluenza Type 3 virus.
  • HN hemagglutinin-neuraminidase
  • monoclonal antibodies against influenza A viruses include MAb 22/1, MAb70/l, MAb 110/1, MAb 264/2, MAb W18/1, MAb 14/3, MAb 24/4, MAb 47/8, MAb 198/2 and MAb 215/2. These monoclonal antibodies are further described in Webster, et al. , Virology, Vol. 96 , pgs 258-264 (1979) . Further examples of monoclonal antibodies against influenza A virus include monoclonal antibodies H2/6A5, H3/4C4, H2/6C4, H2/4B3, H9/B20, H2/4B1, and PEG-1. These monoclonal antibodies are further described in Laver, et al. Proc. Nat. Acad. Sci. , Vol 76, No. 3 , pgs. 1425-1429 (March 1979) . The hereinabove described monoclonal antibodies react against the hemagglutinin protein or the neuraminidase protein of influenza A virus.
  • monoclonal antibodies against influenza B viruses inlcude monoclonal antibodies 113/2 , 124/4, 128/2 , 134/1, 146/1 , 152/2 , 160/1 , 162/1, 195/3, 206/2 , 238/4, and 280/2.
  • the monoclonal antibodies react against the hemagglutinin protein of influenza B virus.
  • Such monoclonal antibodies are further described in Oxford, et al. , J ⁇ Gen. Virol. Vol. 64, pgs 2367-2377 (1983) .
  • Monoclonal antibodies against adenoviruses include monoclonal antibodies 5Hx-l, 5Hx-2 , 5Hx-3, 5Hx-4, 5Hx-5, 5.100K-1 , 5PB-1, and 5Fb-l. Monoclonal antibodies 5Hx-l, 5Hx-2 , 5Hx-3 , 5Hx-4 , and 5Hx-5 were found to react against the hexon polypeptide of adenovirus type 5. Monoclonal antibody 5.100K-1 was found to react against the 100K polypeptide of adenovirus type 5.
  • Monoclonal antibody 5PB-1 was found to react against the penton base polypeptide of adenovirus type 5, and monoclonal antibody 5Fb-l was found to react against the fibre polypeptide of adenovirus type 5.
  • monoclonal antibodies are further described in Russell, et al. , J. Gen. Virol. . Vol. 56, pgs. 393-408 (1981) .
  • a mixture of monoclonal antibodies may be administered against more than one type of respiratory virus .
  • one may administer at least one monoclonal antibody against a respiratory syncytial virus and at least one monoclonal antibody against parainfluenza Type 3 virus for the prophylaxis of respiratory syncytial virus and parainfluenza Type 3 virus.
  • other combinations of monoclonal antibodies may be administered as well, with such combinations depending upon the combination of respiratory viruses one wishes to prevent.
  • the at least one monoclonal antibody when administered to the respiratory tract, either intranasally or by breathing an aerosol containing said at least one monoclonal antibody, is administered in an amount of from about lO ⁇ g to about 250mg, preferably of from about 5mg to about 125mg.
  • a process for the prophylaxis of at least one respiratory virus in an animal which comprises intramuscularly administering to the animal at least one monoclonal antibody against the at least one respiratory virus.
  • the at least one respiratory virus may be selected from those hereinabove described, and the monoclonal antibody or antibodies which may be administered, may be selected from those hereinabove described as well.
  • the at least one monoclonal antibody when administered intramuscularly, is administered in an amount of from about lmg to about 250mg, preferably from about 5mg to about lOOmg.
  • One monoclonal antibody, or a mixture of monoclonal antibodies against one or more respiratory viruses, may be administered.
  • a process for the treatment of at least one respiratory virus in an animal which comprises administering to the animal at least one monoclonal antibody against the at least one respiratory virus.
  • the at least one respiratory virus may be selected from those hereinabove described, and the monoclonal antibody or antibodies which may be administered, may be selected from those hereinabove described as well.
  • One monoclonal antibody, or a mixture of monoclonal antibodies against one or more respiratory viruses may be administered.
  • the at least one monoclonal antibody may be administered topically or systemically. Examples of topical administration include intranasal administration, or administration by breathing an aerosol containing the at least one monoclonal antibody. Examples of systemic administration include intramuscular administration and intravenous administration .
  • the at least one monoclonal antibody when administered topically , is administered to the animal in an amount of from about lO ⁇ g to about 500mg preferably of from about 5mg to about 250mg.
  • the at least one monoclonal antibody is administered in an amount of from about lmg to about 250mg, preferably from about 5mg to about lOOmg.
  • the at least one monoclonal antibody whether administered topically or systemically, and whether administered for the prophylaxis or treatment of at least one respiratory virus, preferably is administered in conjunction with a suitable amount of a pharmaceutical carrier.
  • suitable pharmaceutical carriers include isotonic solutions such as 5% dextrose or 0.9% saline. If a lyopholized preparation is desired , stabilizers such as 0.25M glycine, 10% maltose or 10% sucrose may be included.
  • Example 1 Eight different monoclonal antibodies, each of which is specific for RSV fusion protein (F glycoprotein) , were tested for their ability to block RSV replication when administered to cotton rats by the intranasal route prior to the virus challenge. These antibodies represent seven different epitopes, reacted with at least 13 of 14 virus isolates tested, and retained the greatest neutralizing activity against virus escape mutants selected with the other antibodies. The features of each of the eight antibodies are summarized in Table I below.
  • Cotton rats S. fulviventer
  • Cotton rats in groups of three rats each, average weight 100 grams, were anesthetized with methoxyflurane and given 0.1ml of an antibody solution at a concentration of lOmg/ml or lmg/ml in phosphate buffered saline (PBS) by intranasal (i.n. ) instillation.
  • PBS phosphate buffered saline
  • the antibody was one of the eight monoclonal antibodies hereinabove described, or a pooled mixture of all eight monoclonal antibodies (lOmg/ml total, 1.25mg/ml each or lmg/ml total, 0.125mg/ml each) , or standard human immune globulin (Sandoglobulin, Sandoz, Inc . , East Hanover, N.J. ) .
  • Control rats were given 0.1ml of a bovine serum albumin (BSA) suspension (lOmg/ml or lmg/ml) in PBS .
  • BSA bovine serum albumin
  • Each of the monoclonal antibodies showed a significant reduction in pulmonary virus titer, and were at least as effective and in most cases more effective than the standard immune globulin or the pool of the eight monoclonal antibodies. Five of the eight monoclonal antibodies appear to prevent infection at the high dose (lOmg/ml) and one (1331H) even at the low dose (lmg/ml) .
  • Example 2 Cotton rats (S. fulviventer) in groups of three rats each, average weight 100 grams, were injected with 0.1ml in each flank (0.2ml total) of bovine serum albumin (BSA) , or 0.1ml in each flank (0.2ml total) of either MAb 1129 or MAb 1331H.
  • the monoclonal antibodies are at a concentration of lOmg/ml.
  • the rats were anesthetized with methoxyflurane and challenged by in tra- nasal instillation of 10 5 0 PFU of the Long Strain of RSV.
  • Four days after the virus challenge the rats were sacrificed by carbon dioxide asphyxiation, the lungs were harvested, and the virus content was determined.
  • FIG 2 there was a significant reduction in pulmonary virus titers in rats treated with either of the monoclonal antibodies as compared with the BSA treated rats.
  • Example 3 Cotton rats (S. fulviventer) in groups of three rats each, average weight 100 grams, were anesthetized with methoxyflurane and challenged with 10 5 * 0 PFU of the Long strain of RSV. Three days later, the animals were anesthetized with methoxyflurane and given 0.1ml of a solution of
  • Example 4 The eight monoclonal antibodies described above in Example 1 were further examined for their ability to block RSV replication when administered to cotton rats by the intranasal route prior to virus challenge.
  • Cotton rats S . hispidus, 4 animals per group, average weight 100 grams
  • PBS phosphate-buffered saline
  • Control animals were given 0.1 ml of a bovine serum albumin suspension (1 mg/ml) in PBS .
  • Virus titers were determined using a plaque assay on HEp-2 cell monolayers as disclosed by Prince, et al. , Am. J. Pathol. , Vol. 93, pgs. 771-792 (1978) and are expressed as PFU per gram of tissue.
  • Figure 4 shows that each of the monoclonal antibodies, except MAb 1243, showed a significant reduction in pulmonary virus titer when given at the 0.1 mg or 1 mg doses.
  • Example 5 Neutralizing monoclonal antibodies (MAbs) reactive with the F protein of RSV were examined for effectiveness in blocking RSV replication when combined and administered to cotton rats.
  • MAbs 1121 and 1302 A were mixed in equal amounts.
  • Cotton rats (S. hispidus. 4 animals per group, average weight 100 grams) were anesthetized with methoxyflurane and given 0.1 ml of antibody solution, either the mixture or the individual antibodies at 1 mg/ml, 0.1 mg/ml or 0.01 mg/ml in phosphate -buffered saline (PBS) by intranasal (i.n. ) instillation.
  • Control animals were given 0.1 ml of a bovine serum albumin solution (1 mg/ml) in PBS .
  • Figure 5 shows that MAbs 1121 and 1302A when combined together are more effective in reducing viral titers as compared to their individual effects even at a total concentration of 0.01 mg/ml.
  • Example 6 Six non- neutralizing MAbs, reactive with the F protein of RSV were examined for their ability to block RSV replication when adminsitered to cotton rats by the intranasal route prior to challenge with virus.
  • Cotton rats S . hispidus. 4 animals per group, average weight 100 grams
  • PBS phosphate-buffered saline
  • Control animals were given 0.1 ml of a bovine serum albumin solution (10 mg/ml) in PBS .
  • animals were again anesthetized with methoxyflurane and challenged by i.n.
  • plaque forming units 10 ' plaque forming units (PFU) of the Long strain of RSV.
  • PFU plaque forming units
  • Lungs were harvested and homogenized in 10 parts (wt/vol) of Hanks balanced salt solution supplemented with 0.218 M sucrose, 4.4 mM glutamate, 3.8 mM KH j PO. and 3.2 mM K j HPO, and the resulting suspension was stored at -70°C until assayed for virus content.
  • Virus titers were determined using a plaque assay on HEp-2 cell monolayers as disclosed by Prince et al (1978) and are expressed as PFU per gram of tissue.
  • these non-neutralizing antibodies were effective in significantly decreasing pulmonary viral titers in treated animals even though they do not neutralize virus infectivity in vitro .

Abstract

Procédé de prophylaxie et/ou de traitement de virus respiratoires consistant à administrer localement ou par voie générale, à un animal, au moins un anticorps monoclonal contre au moins un virus respiratoire. L'invention est applicable notamment à la prophylaxie du virus respiratoire syncytial, ou VRS, par administration d'au moins un anticorps monoclonal contre une protéine de fusion du virus respiratoire syncytial.
PCT/US1991/002668 1990-04-19 1991-04-18 Administration d'anticorps monoclonaux contre des virus respiratoires WO1991016074A1 (fr)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0539522A1 (fr) * 1990-07-19 1993-05-05 THE UNITED STATES OF AMERICA as represented by the Secretary United States Department of Commerce Procede immunotherapeutique ameliore pour la prevention ou le traitement des maladies virales des voies respiratoires
EP0671927A1 (fr) * 1992-09-16 1995-09-20 The Scripps Research Institute Anticorps monoclonaux neutralisateurs humains contre le virus respiratoire syncytial
US5534411A (en) * 1993-07-30 1996-07-09 Oravax, Inc. Monoclonal IgA antibody specific for respiratory syncytial virus, a hybridoma cell line that produces this antibody amd methods of using the antibody to diagnose RSV infection
EP1534327A2 (fr) * 2002-07-25 2005-06-01 Medimmune, Inc. Methodes de traitement et de prevention de rsv, hmpv, et piv a l'aide d'anticorps anti-rsv, anti-mpvh, et anti-piv
WO2007002543A3 (fr) * 2005-06-23 2007-07-19 Medimmume Inc Formulations d'anticorps possedant des profils d'agregation et de fragmentation optimises
US7364737B2 (en) 1992-09-16 2008-04-29 The Scripps Research Institute Human neutralizing monoclonal antibodies to respiratory syncytial virus
US7910332B2 (en) 2007-05-25 2011-03-22 Symphogen A/S Method for manufacturing a recombinant polyclonal protein
JP2012233013A (ja) * 2005-05-11 2012-11-29 Alexion Pharmaceuticals Inc 肺疾患を処置するためのモノクローナル抗体の霧状化
WO2015120474A1 (fr) * 2014-02-10 2015-08-13 Igm Biosciences, Inc. Molécules de liaison multispécifiques iga
US9163232B2 (en) 2003-01-07 2015-10-20 Symphogen A/S Method for manufacturing recombinant polyclonal proteins

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4625015A (en) * 1982-08-23 1986-11-25 Scripps Clinic & Research Foundation Broad spectrum influenza antisera
US4719235A (en) * 1984-10-16 1988-01-12 Gerald N. Kern Methods and compositions for treating viral infection
US4800078A (en) * 1987-05-28 1989-01-24 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Immunotherapeutic method of treating respiratory disease by intranasal administration of Igb
US4933169A (en) * 1988-11-23 1990-06-12 Edward Shanbrom Antiviral inhalation therapy
US4965069A (en) * 1985-11-25 1990-10-23 Quash Gerard A Oxidized viruses or viral antigens and utilization for diagnostic prophylactic and/or therapeutic applications

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2631767B2 (ja) * 1990-07-19 1997-07-16 アメリカ合衆国 ウイルス性呼吸器系疾患の予防または治療のためのエーロゾル

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4625015A (en) * 1982-08-23 1986-11-25 Scripps Clinic & Research Foundation Broad spectrum influenza antisera
US4719235A (en) * 1984-10-16 1988-01-12 Gerald N. Kern Methods and compositions for treating viral infection
US4965069A (en) * 1985-11-25 1990-10-23 Quash Gerard A Oxidized viruses or viral antigens and utilization for diagnostic prophylactic and/or therapeutic applications
US4800078A (en) * 1987-05-28 1989-01-24 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Immunotherapeutic method of treating respiratory disease by intranasal administration of Igb
US4933169A (en) * 1988-11-23 1990-06-12 Edward Shanbrom Antiviral inhalation therapy

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
Immunology, Volume 52, issued 1984, G. TAYLOR et al., "Monoclonal antibodies protect against respiratory syncytial virus infection in mice", pages 137-142. See entire document. *
Journal of Virology, Volume 55, No. 3, issued September 1985, G.A. PRIRCE et al., "Quantitative aspects of Passive Immunity to Respiratory Syncytial Virus Infection in Infant Cotton Rats", pages 517-520. See entire document. *
Journal of Virology, Volume 61, No. 6, issued June 1987, G.A. PRINCE et al., Effectiveness of Topically Administered Neutralizing Antibodies in Experimental Immunotherapy of Respiratory Syncytial Virus Infection in Cotton Rats", pages 1851-1854. See entire document. *
Journal of Virology, Volume 62, No. 11, issued November 1988, L.J. ANDERSON et al., "Neutralization of Respiratory Syncytial virus by Individual and Mixtures of F and G Protein monoclonal antibodies", pages 4232-4238. See entire document. *
Journal of Virology, Volume 63, No. 1, issued January 1989, K. VAN WYKE COELINGH et al., "Antigenic and Functional Organization of Human Parainfluenza Virus Type 3 Fusion Glycoprotein", pages 375-382. See entire document. *
Journal of Virology, Volume 63, No. 2, issued January 1989, B. GARCIA-BARRENO et al., "Market Differences in the antigenic structure of Human Respiratory syncytial Virus F and G Glycoproteins", pages 925-932. See entire document. *
See also references of EP0530216A4 *
The Journal of Infectious Diseases, Volume 152, No. 5, issued November 1985, V.G. HEMMING, "Studies of Passive Immunotherapy for Infections of Respiratory Syncytial Virus in the Respiratory Tract of a Primate Model", pages 1083-1087. See entire document. *

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0539522A1 (fr) * 1990-07-19 1993-05-05 THE UNITED STATES OF AMERICA as represented by the Secretary United States Department of Commerce Procede immunotherapeutique ameliore pour la prevention ou le traitement des maladies virales des voies respiratoires
EP0539522A4 (en) * 1990-07-19 1993-09-08 Us Commerce Improved immunotherapeutic method of preventing or treating viral respiratory tract disease
EP0671927A1 (fr) * 1992-09-16 1995-09-20 The Scripps Research Institute Anticorps monoclonaux neutralisateurs humains contre le virus respiratoire syncytial
EP0671927A4 (fr) * 1992-09-16 1997-05-21 Scripps Research Inst Anticorps monoclonaux neutralisateurs humains contre le virus respiratoire syncytial.
US7364737B2 (en) 1992-09-16 2008-04-29 The Scripps Research Institute Human neutralizing monoclonal antibodies to respiratory syncytial virus
US5534411A (en) * 1993-07-30 1996-07-09 Oravax, Inc. Monoclonal IgA antibody specific for respiratory syncytial virus, a hybridoma cell line that produces this antibody amd methods of using the antibody to diagnose RSV infection
EP0724602A1 (fr) * 1993-07-30 1996-08-07 Oravax, Inc. ANTICORPS IgA MONOCLONAL DIRIGE CONTRE LE VIRUS RESPIRATOIRE SYNCYTIAL
EP0724602A4 (fr) * 1993-07-30 1998-12-23 Oravax Inc ANTICORPS IgA MONOCLONAL DIRIGE CONTRE LE VIRUS RESPIRATOIRE SYNCYTIAL
EP1534327A4 (fr) * 2002-07-25 2006-08-23 Medimmune Inc Methodes de traitement et de prevention de rsv, hmpv, et piv a l'aide d'anticorps anti-rsv, anti-mpvh, et anti-piv
EP1534327A2 (fr) * 2002-07-25 2005-06-01 Medimmune, Inc. Methodes de traitement et de prevention de rsv, hmpv, et piv a l'aide d'anticorps anti-rsv, anti-mpvh, et anti-piv
AU2003256823B2 (en) * 2002-07-25 2008-11-06 Medimmune, Llc Methods of treating and preventing RSV, hMPV, and PIV using anti-RSV, anti-hMPV, and anti-PIV antibodies
AU2003256823B9 (en) * 2002-07-25 2009-01-08 Medimmune, Llc Methods of treating and preventing RSV, hMPV, and PIV using anti-RSV, anti-hMPV, and anti-PIV antibodies
US9163232B2 (en) 2003-01-07 2015-10-20 Symphogen A/S Method for manufacturing recombinant polyclonal proteins
JP2012233013A (ja) * 2005-05-11 2012-11-29 Alexion Pharmaceuticals Inc 肺疾患を処置するためのモノクローナル抗体の霧状化
WO2007002543A3 (fr) * 2005-06-23 2007-07-19 Medimmume Inc Formulations d'anticorps possedant des profils d'agregation et de fragmentation optimises
US7910332B2 (en) 2007-05-25 2011-03-22 Symphogen A/S Method for manufacturing a recombinant polyclonal protein
WO2015120474A1 (fr) * 2014-02-10 2015-08-13 Igm Biosciences, Inc. Molécules de liaison multispécifiques iga
US10822399B2 (en) 2014-02-10 2020-11-03 Igm Biosciences, Inc. IgA multi-specific binding molecules

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