WO1991009951A3 - Proteine c recombinee a chaine legere tronquee - Google Patents

Proteine c recombinee a chaine legere tronquee Download PDF

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Publication number
WO1991009951A3
WO1991009951A3 PCT/US1990/007617 US9007617W WO9109951A3 WO 1991009951 A3 WO1991009951 A3 WO 1991009951A3 US 9007617 W US9007617 W US 9007617W WO 9109951 A3 WO9109951 A3 WO 9109951A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
light chain
recombinant protein
mammalian host
activated protein
Prior art date
Application number
PCT/US1990/007617
Other languages
English (en)
Other versions
WO1991009951A2 (fr
Inventor
Donald C Foster
Richard D Holly
Masahiko Suzuki
Kenji Wakabayashi
Anur Ashok Kumar
Original Assignee
Zymogenetics Inc
Teijin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc, Teijin Ltd filed Critical Zymogenetics Inc
Publication of WO1991009951A2 publication Critical patent/WO1991009951A2/fr
Publication of WO1991009951A3 publication Critical patent/WO1991009951A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procédés de production d'une protéine présentant l'activité biologique de la protéine C activée humaine. La protéine est produite par des cellules hôtes mammifères auxquelles on a transmis un plasmide capable d'une intégration dans l'ADN des cellules hôtes mammifères. Le plasmide comprend un activateur suivi en aval par une séquence de nucléotides, laquelle code un précurseur de protéine C activée, lequel est traité afin de produire une protéine présentant l'activité de la protéine C activée humaine, la chaîne légère de la protéine active contenant 149 résidus d'acides aminés.
PCT/US1990/007617 1989-12-22 1990-12-21 Proteine c recombinee a chaine legere tronquee WO1991009951A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45609289A 1989-12-22 1989-12-22
US456,092 1989-12-22

Publications (2)

Publication Number Publication Date
WO1991009951A2 WO1991009951A2 (fr) 1991-07-11
WO1991009951A3 true WO1991009951A3 (fr) 1991-08-22

Family

ID=23811386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/007617 WO1991009951A2 (fr) 1989-12-22 1990-12-21 Proteine c recombinee a chaine legere tronquee

Country Status (2)

Country Link
JP (1) JP3153236B2 (fr)
WO (1) WO1991009951A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230046874A (ko) * 2021-09-30 2023-04-06 (주)케어젠 항노화 활성을 갖는 펩타이드 및 이의 용도

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0266190A2 (fr) * 1986-10-29 1988-05-04 Zymogenetics, Inc. Expression de protéine C
WO1988003926A1 (fr) * 1986-11-17 1988-06-02 New England Medical Center Amelioration de la gamma-carboxylation de proteines recombinantes dependantes de la vitamine k
EP0296413A2 (fr) * 1987-06-12 1988-12-28 Hoechst Japan Limited Protéine C hybride et sa méthode de préparation
EP0319944A2 (fr) * 1987-12-08 1989-06-14 Zymogenetics, Inc. Co-expression dans des cellules eucaryotes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0266190A2 (fr) * 1986-10-29 1988-05-04 Zymogenetics, Inc. Expression de protéine C
WO1988003926A1 (fr) * 1986-11-17 1988-06-02 New England Medical Center Amelioration de la gamma-carboxylation de proteines recombinantes dependantes de la vitamine k
EP0296413A2 (fr) * 1987-06-12 1988-12-28 Hoechst Japan Limited Protéine C hybride et sa méthode de préparation
EP0319944A2 (fr) * 1987-12-08 1989-06-14 Zymogenetics, Inc. Co-expression dans des cellules eucaryotes

Also Published As

Publication number Publication date
JP3153236B2 (ja) 2001-04-03
WO1991009951A2 (fr) 1991-07-11
JPH05506353A (ja) 1993-09-22

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