WO1991007875A1 - Composition for killing the parasitic bee mite varroa jacobsoni - Google Patents

Composition for killing the parasitic bee mite varroa jacobsoni Download PDF

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Publication number
WO1991007875A1
WO1991007875A1 PCT/HU1989/000057 HU8900057W WO9107875A1 WO 1991007875 A1 WO1991007875 A1 WO 1991007875A1 HU 8900057 W HU8900057 W HU 8900057W WO 9107875 A1 WO9107875 A1 WO 9107875A1
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Prior art keywords
killing
linalool
composition
anethole
mite
Prior art date
Application number
PCT/HU1989/000057
Other languages
French (fr)
Inventor
Mária PRIGLI
Jeno^' SUHAYDA
György BÉKÉSSY
Original Assignee
Prigli Maria
Suhayda Jenoe
Bekessy Gyoergy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prigli Maria, Suhayda Jenoe, Bekessy Gyoergy filed Critical Prigli Maria
Priority to PCT/HU1989/000057 priority Critical patent/WO1991007875A1/en
Publication of WO1991007875A1 publication Critical patent/WO1991007875A1/en

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/14Ethers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/16Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/28Myrtaceae [Myrtle family], e.g. teatree or clove

Definitions

  • the invention relates to composition for killing the parasitic bee mite Varroa jacobsoni.
  • the Asian parasitic bee mite (Varroa jacobsoni) is a very serious problem in beekeeping and several methods and substances had been developed for controlling it.
  • a physical method of mite control includes heat treatment of the colonies at temperatures of from 46 to 20 48 °C. But this is a very risky and costly method as the bees may easily be killed if the temperature rises to 9 or 50 °C.
  • Chemical treatments are also known. These use chemical substances in dust or spray form which may 25 easily have access to the final product honey, rendering such honey unfit for human consumption.
  • the object of the present invention is to provide a composition which is effective in killing the mites but will not affect the life and health of the colony and will not affect the quality of honey produced.
  • the invention is based on the recognition that mites can be effectively killed with compositions containing one or more acyclic or cyclic terpenes, preferably linalool linalyl acetate, eugenol and/or anethole or the volatile oil of plants containing one or more acyclic or cyclic terpenes, preferably linalool, linalyl acetate, eucalyptol, eugenol and/or anethole or a mixture of these volatile oils or a mixture of these volatile oils and the terpenes mentioned above.
  • acyclic or cyclic terpenes preferably linalool linalyl acetate, eugenol and/or anethole or the volatile oil of plants containing one or more acyclic or cyclic terpenes, preferably linalool, linalyl acetate, eucalyptol, eugenol and/
  • compositions of-the present invention can be prepared in forms suitable for evaporation, spraying or fumigation.
  • compositions for mite control usually contain from 0.001 to 1 by weight of active ingredient, one or more usual carriers and if desired other known additives.
  • compositions contain as active ingredient anethole, linalool or linalyl acetate or volatile oil of plants containing them or their mixture.
  • oil of lavender As volatile oil of plants the following are preferred: oil of lavender, bergamot oil, oil of clove, :. anise oil.
  • the main constituents of oil of lavender are linalool and linalyl acetate, while bergamot oil contains linalyl acetate as its major component.
  • Oil of clove contains mainly eugenol, while the major component of anise oil is anethole.
  • the advantage of the compositions of the present invention is that they contain natural substances as active ingredient, by the use of the composition bee mites can be effectively killed and they are harmless to bees and to the quality of honey produced.
  • the substances used and the results obtained are the following: linalool: the mite left the bee after 7 minutes, the. mite died after 12 minutes; oil of clove: the mite left the bee after 15 minutes, the mite died after 18 minutes; anise oil: the mite left the bee after minutes, the mite died after 30 minutes; oil of lavender: the mite left the bee after 5 minutes, the mite died after 20 minutes; linalyl acetate: the mite left the bee after 7 minutes, the mite died after 42 minutes; anethole: the mite left the bee after 6 minutes, the mite died after 40 minutes; 1 to 1 mixture (by volume) of oil of lavender and linaloo the mite left the bee after 9 minutes, the mite died after 10 minutes.
  • Evaporation plates of 20 x 30 cm size were made from the mixture of one part of gypsum and two parts of diatomite. The plates were placed into the hives and 5 ⁇ 5 ml of the substances to be tested were dropped on the plates. After 21 or 23 days the killed mites were counted and the killing effect was calculated. The results are summarized in Table 1.
  • the active ingredient of the Varrescens strip is Amitraz.
  • Test 5 was repeated in -hives, the substances to be tested were used in an amount of 0.1 ml. At the time of the test the outside temperature was 7 °C.
  • compositions of the present invention are illustrated by the following Examples:
  • Example A Evaporation plate a) On a 20 x 30 cm Jerusalem plate (thickness: 2 mm) 5 ml of oil of lavender are applied; b) On a 20 x 30 cm (thickness: 5 mm) plate made by mixing one part of gypsum with 2 parts of diato ite, 5 ml of a l:l/v:v of anethole - ethanol mixture are applied. c) On a 20 x 30 cm (thickness: 5 mm) plate made by mixing one part of gypsum with 2 parts of diatomite, 5 ml of a l:l/v:v of linalool — paraffin oil mixture are applied.
  • a 3 x 10 cm paper strip is impregnated with a 10 % by weight aqueous potassium nitrate solution. On this paper strip 0.15 ml of anethole is applied.
  • Fumigation pill or pellet 10 mg of linalool, 90 mg of cyclodextrin and 5 mg of potassium nitrate are mixed.
  • linalool Two parts (by volume) of linalool are mixed with one part of ethanol; Prior to use this concentrate is diluted with an aqueous sugar syrup up to the desired concentration.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a composition for killing the parasitic bee mite Varroa Jacobsoni containing as active ingredient one or more cyclic or acyclic terpenes, preferably linalool, linalyl acetate, eugenol and/or anethole or the volatile oil of plants containing them or their mixture.

Description

C0XP0SITI0N FOR KILLING THE PARASITIC BEE MITE VARROA J COBSONI
The invention relates to composition for killing the parasitic bee mite Varroa jacobsoni.
5 Background of the invention
The Asian parasitic bee mite (Varroa jacobsoni) is a very serious problem in beekeeping and several methods and substances had been developed for controlling it.
Among such methods the biological methods of 10 control make use of the knowledge of bee and Varroa biology and attack the mites where they are vulnerable. Such methods are: regularly removing the drone brood from the bee families, removing the brood at regular intervals and keeping it in incubators for the rest of time. These 15 methods of mite control require however the highest skills, sophisticated equipment and both much effort and a highly concentrated working.
A physical method of mite control includes heat treatment of the colonies at temperatures of from 46 to 20 48 °C. But this is a very risky and costly method as the bees may easily be killed if the temperature rises to 9 or 50 °C.
Chemical treatments are also known. These use chemical substances in dust or spray form which may 25 easily have access to the final product honey, rendering such honey unfit for human consumption.
Substances which should be eaten by the bees are usually dissolved in sugar syrup and then sprayed onto bees; they are swallowed by the bees licking each other. One such substance is Perisine (Ritter, ¥. : Tierartzlicher Umschau, 40 /I/, 14-15, 1985) which has very favourable effects but can easily be passed over to the honey. Therefore, Perisine cannot be used when the colony is collecting honey.
Another method of control is fumigation using substances like bromine propylate (Poltev, V.J. - Likhotin, A.K=: Veterinarija, 11, 60-51, 1975) or Amitraz (Kilani et al.: Apidologie, 12 /I/, 31-36, 1981). The use of these substances bears many risks as these chemicals are toxic, the risks are the following: inhaling the gases when the fumigation stripes are being lit is highly dangerous to humans, when using these chemicals" obligatory wait periods are to be kept as determined by foodstuff legislation, these chemicals cannot be used during the 14-day period prior to extracting the honey from the honeycomb, these chemicals should be inserted or introduced preferably in the evening when all bees are again in the hive, during summer bees gst more aggressive after the application of the chemicals.
In some control methods chemicals like formic acid are evaporated. 10 ml of formic acid has to be evapora d daily, but it is difficult to controll the rate of evapc ation. When the rate of the evaporation of formic acid is too high, the bees may be killed very easily.
The object of the present invention is to provide a composition which is effective in killing the mites but will not affect the life and health of the colony and will not affect the quality of honey produced.
Brief description of the invention
The invention is based on the recognition that mites can be effectively killed with compositions containing one or more acyclic or cyclic terpenes, preferably linalool linalyl acetate, eugenol and/or anethole or the volatile oil of plants containing one or more acyclic or cyclic terpenes, preferably linalool, linalyl acetate, eucalyptol, eugenol and/or anethole or a mixture of these volatile oils or a mixture of these volatile oils and the terpenes mentioned above.
Detailed description of the invention
The compositions of-the present invention can be prepared in forms suitable for evaporation, spraying or fumigation.
According to the invention the ready to use compositions for mite control usually contain from 0.001 to 1 by weight of active ingredient, one or more usual carriers and if desired other known additives.
The preferred compositions contain as active ingredient anethole, linalool or linalyl acetate or volatile oil of plants containing them or their mixture.
As volatile oil of plants the following are preferred: oil of lavender, bergamot oil, oil of clove, :. anise oil. The main constituents of oil of lavender are linalool and linalyl acetate, while bergamot oil contains linalyl acetate as its major component. Oil of clove contains mainly eugenol, while the major component of anise oil is anethole. The advantage of the compositions of the present invention is that they contain natural substances as active ingredient, by the use of the composition bee mites can be effectively killed and they are harmless to bees and to the quality of honey produced.
The following tests show the activity of the compositions of the present invention.
Test 1
Laboratory evaporation test In Petri-dishes of 10 cm diameter filter paυers each
2 of 2 cm surface were placed and the substances to be tested were dropped thereon (20 ul on each piece of pape^.
Then in each Petri-dish 3 bees each infested with one
Varroa jacobsoni. mite were-_placed and the behaviour of both bees and mites was observed.
The substances used and the results obtained are the following: linalool: the mite left the bee after 7 minutes, the. mite died after 12 minutes; oil of clove: the mite left the bee after 15 minutes, the mite died after 18 minutes; anise oil: the mite left the bee after minutes, the mite died after 30 minutes; oil of lavender: the mite left the bee after 5 minutes, the mite died after 20 minutes; linalyl acetate: the mite left the bee after 7 minutes, the mite died after 42 minutes; anethole: the mite left the bee after 6 minutes, the mite died after 40 minutes; 1 to 1 mixture (by volume) of oil of lavender and linaloo the mite left the bee after 9 minutes, the mite died after 10 minutes.
In all Petri-dishes the bees remained healthy.
Test 2
Evaporation test in modular hives
Evaporation plates of 20 x 30 cm size were made from the mixture of one part of gypsum and two parts of diatomite. The plates were placed into the hives and 5~5 ml of the substances to be tested were dropped on the plates. After 21 or 23 days the killed mites were counted and the killing effect was calculated. The results are summarized in Table 1.
, Table 1 Active ingredient Duration of Mite killing treatment (days) effect (#)
A 21 88 B 21 93 C 21 70 D 23 85
A l:l/v:v mixture of oil of lavender and linalool, B l:l/v:v mixture of anise oil and oil of lavender, C oil of lavender, D anise oil. Remark: After each treatment the colony remained calm and healthy, and the brood was not deformed or otherwise affected, and adult bees hatched from this brood were healthy. Test 3
Laboratory feeding test
1 % and l#o solutions of anethole, linalool and oil of lavender were prepared with 50 by weight aqueous sugar syrup. 20-20 bees infested with mites were fed with these solutions for 15 days. It was observed that after 2 or 3 days mites started to die, all mites died within 15 days. The treatment had no harmful effect on the bees.
Test 4
Spraying test in modular hives
1 solutions of anethole, linalyl acetate and linalool were prepared with 50 by wei^nt aqueous sugar syrup and the solutions were sprayed on mite-infested bees being on the frames or into the spaces between honeycombs. 150 ml of the spray solution were applied to each three storied modular hive. On the day after treatment the dead mites and bees, if any, were counted and the activity was calculated.
The results thus obtained were then compared to the results obtained by using the commercially available "Varrβscens" fumigation strips, the activity of the Varrescens strips was assumed to be 100 . The results are shown in Table 2.
Figure imgf000009_0001
Remark: The active ingredient of the Varrescens strip is Amitraz.
No bee was harmed during these treatments and no robbery activities could be observed.
Test 5
Laboratory fumigation test Mite-infested bees were placed in queen cages and the cages were placed in 5-litre glass containers. On a wringled piece of paper towel (size: 8 x 8 mm) 0.1 ml and 0.05 ml of the substances to be tested were dropped. The paper towel pieces thus treated were placed into a bee smoker and burned. Prom the fumes 10 or 12 blasts were introduced into the glass containers which were then covered with a glass plate. The glass plate cover had been removed after 10 minutes. The number of the dead mites (and bees) was counted after 24 hours. The results are shown in Table 3. Table 3
Active Amount of Number of Dying of Living ingredient the active bees mites the mites bees mite ingredient at the beginning started at the en (ml) of the test after of the tes minutes
Anethole 0.1 14 10 5 0.05 10 8 10
Linalyl 0.1 8 3 10 acetate
0.05 9 9 10 Linalool 0.1 12 9 10
0.05 11 3 10
Figure imgf000010_0001
Test 6
Fumigation test in hives
Test 5 was repeated in -hives, the substances to be tested were used in an amount of 0.1 ml. At the time of the test the outside temperature was 7 °C.
After one fumigation in case of anethole the number of the deed mites was 9.75 this number was 3.0 for linalyl acetate and 5-1 fo linalool. No bee was killed in these tests, the average activity was 90 °> .
These tests were repeated by applying 0.1 ml of each of the above active ingredients. The activity increased to 99 no bee was killed.
The compositions of the present invention are illustrated by the following Examples:
Example A Evaporation plate a) On a 20 x 30 cm fait plate (thickness: 2 mm) 5 ml of oil of lavender are applied; b) On a 20 x 30 cm (thickness: 5 mm) plate made by mixing one part of gypsum with 2 parts of diato ite, 5 ml of a l:l/v:v of anethole - ethanol mixture are applied. c) On a 20 x 30 cm (thickness: 5 mm) plate made by mixing one part of gypsum with 2 parts of diatomite, 5 ml of a l:l/v:v of linalool — paraffin oil mixture are applied.
Example B Fumigation mixture a) Fumigation strip
A 3 x 10 cm paper strip is impregnated with a 10 % by weight aqueous potassium nitrate solution. On this paper strip 0.15 ml of anethole is applied. b) Fumigation pill or pellet 10 mg of linalool, 90 mg of cyclodextrin and 5 mg of potassium nitrate are mixed.
Example C Spraying mixture
To 1 litre of aqueous sugar syrup containing 50 sugar 2 ml of linalool and l.ml of ethanol are added.
Example D
Spray concentrate
Two parts (by volume) of linalool are mixed with one part of ethanol; Prior to use this concentrate is diluted with an aqueous sugar syrup up to the desired concentration.

Claims

Claims
1. Composition for killing the parasitic bee mite Varroa jacobsoni characterized by containing as active ingredient one or more acyclic or cyclic terpenes , preferably linalool, linalyl acetate, eugenol and/or anethole together with one or more carriers and/or other known additives.
2. Composition for killing the parasitic bee mite Varroa jacobsoni characterized by containing as active ingredient the volatile oil of plants containing one or more acyclic or cyclic terpenes, preferably linalool, linalyl acetate, eugenol and/or anethole or a mixture of these volatile oils together with one or more carriers and/or other known aditives.
3. Composition for 'killing the parasitic bee mite Varroa jacobsoni characterized by containing as active ingredient a mixture of 4one or more acyclic or cyclic terpenes, preferably of linalool, linalyl acetate, eugenol and/or anethole and volatile oils of plants containing them together with one or more carriers and/or other known additives.
4. The composition according to Claim 1, characterized by containing as active ingredient anethole, linalool or linalyl acetate.
5. The composition according to Claim 2, characterized by containing as active ingredient anise oi or lavender oil.
PCT/HU1989/000057 1989-11-27 1989-11-27 Composition for killing the parasitic bee mite varroa jacobsoni WO1991007875A1 (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2695539A1 (en) * 1992-09-17 1994-03-18 Desowag Materialschutz Gmbh Temporary action product to fight termites in the soil.
WO2000021364A2 (en) * 1998-10-09 2000-04-20 Auburn University A natural and safe alternative to fungicides, bacteriocides, nematicides and insecticides for plant protection and against household pests
EP2301357A2 (en) 2009-08-31 2011-03-30 Gilles. M Grosmond Biological composition against Varroa
FR2964302A1 (en) * 2010-09-08 2012-03-09 Gilles Grosmond Biological composition, useful for ensuring a better tolerance of bee products administered in hives, comprises a mixture of oils essential or propolis or synthetic molecules similar to essential oils
RU2496310C1 (en) * 2012-06-22 2013-10-27 Общество с ограниченной ответственностью "УНИФАРМ" (ООО "УНИФАРМ") Agent for prevention and treatment of bees and bee brood
CN104839151A (en) * 2015-04-01 2015-08-19 金华市农业科学研究院 Application of Cis-anethol as drug to prevent and treat bee mites
WO2020132758A1 (en) * 2018-12-28 2020-07-02 Pontificia Universidad Catolica De Chile Organic pesticide composition of native chilean plants for application in beekeeping and agronomy
WO2024102088A1 (en) * 2022-11-10 2024-05-16 İsti̇nye Üni̇versi̇tesi̇ Hive vials containing essential oil for the prevention and treatment of diseases of honey bees

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GB2163651A (en) * 1984-07-26 1986-03-05 Riviana Foods Inc Linalool pesticide
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GB2163651A (en) * 1984-07-26 1986-03-05 Riviana Foods Inc Linalool pesticide
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GB2178315A (en) * 1985-08-02 1987-02-11 Univ Southampton Insect attractant comprising linalool
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Title
CHEMICAL ABSTRACTS, Volume 107, No. 11, 89 361s, issued 14 September 1987, (Columbus, Ohio, USA), D. POPESKOVIC et al., "The Blockade of Hemocyanin Function of the Mite Varroa Jacobsoni as a Specific Physiological Basis for Systemic Treatment of Honeybee Varroatosis"; & C.R. SEANCES SOC. BIOL. SES. FIL. 1986, 180(6), 663-8, see page 27. *
CHEMICAL ABSTRACTS, Volume 107, No. 25, 235 042g, issued 21 December 1987, (Columbus, Ohio, USA), W. STOYA et al., "Lactic Acid for the Treatment of Varroatosis and its Effect on Honey"; & DTSCH. LEBENSM.-RUNDSCH. 1987, 83(9), 283-6, see page 647. *
SOVIET INVENTIONS ILLUSTRATED, Section Ch, Week J51, issued 9 February 1983, by DERWENT PUBLICATION LTD. (London). *

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WO2000021364A3 (en) * 1998-10-09 2000-08-10 Univ Auburn A natural and safe alternative to fungicides, bacteriocides, nematicides and insecticides for plant protection and against household pests
EP2301357A2 (en) 2009-08-31 2011-03-30 Gilles. M Grosmond Biological composition against Varroa
FR2964302A1 (en) * 2010-09-08 2012-03-09 Gilles Grosmond Biological composition, useful for ensuring a better tolerance of bee products administered in hives, comprises a mixture of oils essential or propolis or synthetic molecules similar to essential oils
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